Click here to switch to the map view.

The map label for this gene is thcD [H]

Identifier: 221636242

GI number: 221636242

Start: 821086

End: 822300

Strand: Reverse

Name: thcD [H]

Synonym: trd_A0836

Alternate gene names: 221636242

Gene position: 822300-821086 (Counterclockwise)

Preceding gene: 221636244

Following gene: 221636236

Centisome position: 89.6

GC content: 67.16

Gene sequence:

>1215_bases
ATGGTCGAGCACTGCCTCGTCGTGGGAGCTGGAGCGGCAGCCTTCGGGTTCGCGAGTGCGATCCGCGAGGCGGGGTACCA
GGGACGGGTCACTGTGGTGGGCGCCGAGCCGGTGCCACCATACGAGCGACCACCCCTTTCCAAGCAGTATCTCCTGGGGA
AGAAAACGGAGAGCGAGATACTCTTCCGGCCGGTCGCGTTCTACGAGGAACGGACGATCGAGCTGGTTCTGGGTGATCCG
GTCGAGGAGATCGATCTCGAGCGCCGGGTGGCCCGCACGCGTGCCGCGAGGGAGATCGGCTTCGATCAGTTGGTGCTGGC
AACTGGGGCGGTACCGAACCGGCTGGCGGTACCGGGGGCAGAACTCCCGGGGGTCTTCGTGCTTCGCTCGCTCGAGGACG
CACGAGGGGTGCGAGCGGCGCTGTCGTCTGCGCAGCGCGTCGTCGTGATCGGTGGCGGGTTCATCGGGTGCGAAGTCGCG
GCGAGCGCGCGGACATTGGGGAAGCAGGTTGCGCTGGTCGAAACGTTGCCAGTTCTGCTCGGTCGAGCGCTCGGCGAGAC
GATCGGAGCCGCGATCACGCGGGTGCACGAGCGAGCTGGTGTGGAACTCCACCTGGGGCGGACGGTGATCGCATTGGAGG
GGCGAGAGCGGGTGGAGCGGGTTCTCCTGGACGACGGCACGAGCCTCCCCGCCGAGACCGTCATCGTCGGGATCGGCGTC
CGGCCAGCCGTGCCGGCGATCAGGGGCGAGCTGACGATAGAGGACGGTGTGGTCGTCGATGCGACCTGCGCGGCGAGTGT
CCCGGGCGTCTGGGCCGCCGGTGACGTGGCGCGCTGGTGGCATCCGGTGATCGAGCGCTCGATTCGGGTCGAGCACTTCG
ACAACGCGTTGGCGCAAGGTGCGTGCGTCGCCAAGGGAGTCGCTGGGCGTCCCGAGACGTACGCGCCGGTACCGTCTTTC
TGGTCGGATCAGTACGATCTGACCATCCAGCAGTACGGTTACCCGATCGAATGGGATGAACTCGTCGTGCGTGGCGATCT
GGACGCTCCGTCCTTCACAGCCTTCTACTTAAAAGACGGGCGTGTTTGCGGGGCAGTGATCGTGAAGCGGCCGCGCGAGA
TGCGACCAGCACGGCGCCTGGTGGAGGCGATGGCGCGAGTCGATCGCGCGCTCCTCGCGGACGAGCGTGTCGATCTGCGT
TCGCTCGCGCCTTGA

Upstream 100 bases:

>100_bases
GCATGCGGGGGCCGCTGGCGTCGTCGACCGGGGAACGACGGTCGCGACGAGGAAGAAGCGGGTGCTCGGGCGCTGATCCG
GTGCGCTGAAGGGGGTTGCC

Downstream 100 bases:

>100_bases
ATCGAAATCCTGGTCCCGTCGGAAGCCGGCGAGGAGGGGAAACGGGGACCAGCGAGCGGTGAGTCAGCTTGGGTCTCGGC
GGCGAGAGCGCCGAGCTTCC

Product: putidaredoxin reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MVEHCLVVGAGAAAFGFASAIREAGYQGRVTVVGAEPVPPYERPPLSKQYLLGKKTESEILFRPVAFYEERTIELVLGDP
VEEIDLERRVARTRAAREIGFDQLVLATGAVPNRLAVPGAELPGVFVLRSLEDARGVRAALSSAQRVVVIGGGFIGCEVA
ASARTLGKQVALVETLPVLLGRALGETIGAAITRVHERAGVELHLGRTVIALEGRERVERVLLDDGTSLPAETVIVGIGV
RPAVPAIRGELTIEDGVVVDATCAASVPGVWAAGDVARWWHPVIERSIRVEHFDNALAQGACVAKGVAGRPETYAPVPSF
WSDQYDLTIQQYGYPIEWDELVVRGDLDAPSFTAFYLKDGRVCGAVIVKRPREMRPARRLVEAMARVDRALLADERVDLR
SLAP

Sequences:

>Translated_404_residues
MVEHCLVVGAGAAAFGFASAIREAGYQGRVTVVGAEPVPPYERPPLSKQYLLGKKTESEILFRPVAFYEERTIELVLGDP
VEEIDLERRVARTRAAREIGFDQLVLATGAVPNRLAVPGAELPGVFVLRSLEDARGVRAALSSAQRVVVIGGGFIGCEVA
ASARTLGKQVALVETLPVLLGRALGETIGAAITRVHERAGVELHLGRTVIALEGRERVERVLLDDGTSLPAETVIVGIGV
RPAVPAIRGELTIEDGVVVDATCAASVPGVWAAGDVARWWHPVIERSIRVEHFDNALAQGACVAKGVAGRPETYAPVPSF
WSDQYDLTIQQYGYPIEWDELVVRGDLDAPSFTAFYLKDGRVCGAVIVKRPREMRPARRLVEAMARVDRALLADERVDLR
SLAP
>Mature_404_residues
MVEHCLVVGAGAAAFGFASAIREAGYQGRVTVVGAEPVPPYERPPLSKQYLLGKKTESEILFRPVAFYEERTIELVLGDP
VEEIDLERRVARTRAAREIGFDQLVLATGAVPNRLAVPGAELPGVFVLRSLEDARGVRAALSSAQRVVVIGGGFIGCEVA
ASARTLGKQVALVETLPVLLGRALGETIGAAITRVHERAGVELHLGRTVIALEGRERVERVLLDDGTSLPAETVIVGIGV
RPAVPAIRGELTIEDGVVVDATCAASVPGVWAAGDVARWWHPVIERSIRVEHFDNALAQGACVAKGVAGRPETYAPVPSF
WSDQYDLTIQQYGYPIEWDELVVRGDLDAPSFTAFYLKDGRVCGAVIVKRPREMRPARRLVEAMARVDRALLADERVDLR
SLAP

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=366, Percent_Identity=30.6010928961749, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI226437568, Length=366, Percent_Identity=30.6010928961749, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI65787454, Length=366, Percent_Identity=30.6010928961749, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI22202629, Length=339, Percent_Identity=26.2536873156342, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4757732, Length=339, Percent_Identity=26.2536873156342, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1788892, Length=403, Percent_Identity=32.0099255583127, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1789065, Length=280, Percent_Identity=30, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1789765, Length=265, Percent_Identity=27.1698113207547, Blast_Score=102, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17559934, Length=364, Percent_Identity=28.5714285714286, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24639257, Length=367, Percent_Identity=26.7029972752044, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI281359715, Length=367, Percent_Identity=26.7029972752044, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI281359713, Length=367, Percent_Identity=26.7029972752044, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24639250, Length=367, Percent_Identity=26.7029972752044, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI18543267, Length=367, Percent_Identity=26.7029972752044, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24639252, Length=367, Percent_Identity=26.7029972752044, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24585130, Length=362, Percent_Identity=25.9668508287293, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24581020, Length=357, Percent_Identity=26.0504201680672, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI28573993, Length=357, Percent_Identity=26.0504201680672, Blast_Score=108, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43544; Mature: 43544

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEHCLVVGAGAAAFGFASAIREAGYQGRVTVVGAEPVPPYERPPLSKQYLLGKKTESEI
CCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHCCCCCCCHH
LFRPVAFYEERTIELVLGDPVEEIDLERRVARTRAAREIGFDQLVLATGAVPNRLAVPGA
EEEHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCCCC
ELPGVFVLRSLEDARGVRAALSSAQRVVVIGGGFIGCEVAASARTLGKQVALVETLPVLL
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHH
GRALGETIGAAITRVHERAGVELHLGRTVIALEGRERVERVLLDDGTSLPAETVIVGIGV
HHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCHHHHHHHHHCCCCCCCCCCEEEECCC
RPAVPAIRGELTIEDGVVVDATCAASVPGVWAAGDVARWWHPVIERSIRVEHFDNALAQG
CCCCHHHCCCEEEECCEEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHEEHHHHHHHHHCC
ACVAKGVAGRPETYAPVPSFWSDQYDLTIQQYGYPIEWDELVVRGDLDAPSFTAFYLKDG
CHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCCCCHHHEEEEECCCCCCEEEEEEECC
RVCGAVIVKRPREMRPARRLVEAMARVDRALLADERVDLRSLAP
CEEEHHHHCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MVEHCLVVGAGAAAFGFASAIREAGYQGRVTVVGAEPVPPYERPPLSKQYLLGKKTESEI
CCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHCCCCCCCHH
LFRPVAFYEERTIELVLGDPVEEIDLERRVARTRAAREIGFDQLVLATGAVPNRLAVPGA
EEEHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCCCC
ELPGVFVLRSLEDARGVRAALSSAQRVVVIGGGFIGCEVAASARTLGKQVALVETLPVLL
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHH
GRALGETIGAAITRVHERAGVELHLGRTVIALEGRERVERVLLDDGTSLPAETVIVGIGV
HHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCHHHHHHHHHCCCCCCCCCCEEEECCC
RPAVPAIRGELTIEDGVVVDATCAASVPGVWAAGDVARWWHPVIERSIRVEHFDNALAQG
CCCCHHHCCCEEEECCEEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHEEHHHHHHHHHCC
ACVAKGVAGRPETYAPVPSFWSDQYDLTIQQYGYPIEWDELVVRGDLDAPSFTAFYLKDG
CHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCCCCHHHEEEEECCCCCCEEEEEEECC
RVCGAVIVKRPREMRPARRLVEAMARVDRALLADERVDLRSLAP
CEEEHHHHCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]