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The map label for this gene is proP [H]

Identifier: 221636235

GI number: 221636235

Start: 815354

End: 816724

Strand: Reverse

Name: proP [H]

Synonym: trd_A0829

Alternate gene names: 221636235

Gene position: 816724-815354 (Counterclockwise)

Preceding gene: 221636236

Following gene: 221636234

Centisome position: 88.99

GC content: 63.38

Gene sequence:

>1371_bases
GTGTTCGGGAAGGGAGAGGGCACGATGCGTGCGGTGAACAACGATCGGCGCTATCTGGCCTTCGTGATCGGCGCCTCGAC
GGTCGGGTCGGCGATCGAGTGGTATGACTTCTACATTTTCGGGAGCTTGGCGGCGATTCTGGCGACGCATTTCTTCGCCA
AGGACCACCCGGTCGCGGCGCTCCTGAATACCATCGCGATCTTTACCATCGGGTTCCTGTTCCGGCCACTCGGGGCGATC
ATCTTCGGCTGGTACGGGGACAAGATCGGGCGCAAGCGGGTCTTCATCCTGACCTTGCTCGGGATGGGTTTGGGGACGGC
ACTGATCGGCGTGATCCCGAGCTACGCGACGATCGGGGTGCTCGCTCCCATCTTGGTCTTTTTGTTGCGGGTGATCCAGG
GGTTGAGTGTCGGGGGTGAGTGGGGAGGTGCGCTGACCTACGTGGCCGAGCACGCGCCGGACGGGAAGCGTGGGCTGTAC
ACGGGTCTTTTGAGCCCGGCGGCGATCTACGGTCTGATCATCTCGTTCTTCGTGGTGCTGGGTGTGCGGACGGCGCTCGG
TGAGGAGGCGTTTCGAGCCTGGGGCTGGCGCATACCGTTCCTGCTTTCGCTTGTGCTGGTCCTGATCTCGCTCTACGTGC
GGCTGCGTCTCCAGGAGACGCCGATGTTCGCTGAACTGAAGGCGCGGCGTGCAGTGGTGCAGAATCCCTTGAAGGTGACC
TTCACGGGCGAGAACTTGCGGCGGGTACTCATTGCGGCAGCCGTCTCGTTCGGTCAAGCGGTGGCCTGGTATACCGCGAT
TTTCTGGGCGCTCTTCTTCCTGCAGACCGCGCAGAAGCTCGATCTCATCACGACGTACAAGGTCCTTCTGCCGGGTCTGC
TGGTGGCGCCAGTGCTGCATATCGTCATGGGCTGGCTCTCCGACTCGATCGGCCGCAAGCCGATCGTCCTCACTGGCTTC
GTCCTGGCGATCGTCGGCTTCTACCCACTCTATACGCTGCTCGGGCGGTTCGCGCAGAAGGACAATCTGCAGTACTGGCC
GGCCGCCCTGACCGTGATGGGCCTGATGCTGATCGCGGCGATGGTGTGGGGACCGCTGCCAGCTCTGTTGGCCGAGTACT
TCCCCACGCAGGTCCGTTATACCTCCCTGGGCGTCGTCCAGAACGCGGCCAATGGCTGGGGCGGTGGACTGGTCCCGTAC
GTCACGACGTGGATCCAGACTGCGACCGGAAACTTGATGTGGGCGCTGGTCTATCCGATCGCCTTGCCGCTCCTGGGGTT
GATCGTCGGTCTCCTGTTCTTGCCCGAGACGGCGCGCCACCGCTTGTGGGAGCAGCCAGGGGCGGCGATGGCGACCGGCG
ACGGGGAATAA

Upstream 100 bases:

>100_bases
ATTCGATGCATCACTGATGTGTGCAGCCGCTGTGACTCGTTGTAATGCTGTGATGAACGCGTGATAGGGTCAGACGTCGG
CAAGGAGGAGATAGAGTCGA

Downstream 100 bases:

>100_bases
TTCGGATACTAGGACGACGATGCGATTGGCCGGTTCAGCGAGTTGTCGCCCCGGCCGGTCGCTATCCGCTCGCAAGAGGC
GGAGGGAGATGATGATGCAG

Product: major facilitator superfamily transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MFGKGEGTMRAVNNDRRYLAFVIGASTVGSAIEWYDFYIFGSLAAILATHFFAKDHPVAALLNTIAIFTIGFLFRPLGAI
IFGWYGDKIGRKRVFILTLLGMGLGTALIGVIPSYATIGVLAPILVFLLRVIQGLSVGGEWGGALTYVAEHAPDGKRGLY
TGLLSPAAIYGLIISFFVVLGVRTALGEEAFRAWGWRIPFLLSLVLVLISLYVRLRLQETPMFAELKARRAVVQNPLKVT
FTGENLRRVLIAAAVSFGQAVAWYTAIFWALFFLQTAQKLDLITTYKVLLPGLLVAPVLHIVMGWLSDSIGRKPIVLTGF
VLAIVGFYPLYTLLGRFAQKDNLQYWPAALTVMGLMLIAAMVWGPLPALLAEYFPTQVRYTSLGVVQNAANGWGGGLVPY
VTTWIQTATGNLMWALVYPIALPLLGLIVGLLFLPETARHRLWEQPGAAMATGDGE

Sequences:

>Translated_456_residues
MFGKGEGTMRAVNNDRRYLAFVIGASTVGSAIEWYDFYIFGSLAAILATHFFAKDHPVAALLNTIAIFTIGFLFRPLGAI
IFGWYGDKIGRKRVFILTLLGMGLGTALIGVIPSYATIGVLAPILVFLLRVIQGLSVGGEWGGALTYVAEHAPDGKRGLY
TGLLSPAAIYGLIISFFVVLGVRTALGEEAFRAWGWRIPFLLSLVLVLISLYVRLRLQETPMFAELKARRAVVQNPLKVT
FTGENLRRVLIAAAVSFGQAVAWYTAIFWALFFLQTAQKLDLITTYKVLLPGLLVAPVLHIVMGWLSDSIGRKPIVLTGF
VLAIVGFYPLYTLLGRFAQKDNLQYWPAALTVMGLMLIAAMVWGPLPALLAEYFPTQVRYTSLGVVQNAANGWGGGLVPY
VTTWIQTATGNLMWALVYPIALPLLGLIVGLLFLPETARHRLWEQPGAAMATGDGE
>Mature_456_residues
MFGKGEGTMRAVNNDRRYLAFVIGASTVGSAIEWYDFYIFGSLAAILATHFFAKDHPVAALLNTIAIFTIGFLFRPLGAI
IFGWYGDKIGRKRVFILTLLGMGLGTALIGVIPSYATIGVLAPILVFLLRVIQGLSVGGEWGGALTYVAEHAPDGKRGLY
TGLLSPAAIYGLIISFFVVLGVRTALGEEAFRAWGWRIPFLLSLVLVLISLYVRLRLQETPMFAELKARRAVVQNPLKVT
FTGENLRRVLIAAAVSFGQAVAWYTAIFWALFFLQTAQKLDLITTYKVLLPGLLVAPVLHIVMGWLSDSIGRKPIVLTGF
VLAIVGFYPLYTLLGRFAQKDNLQYWPAALTVMGLMLIAAMVWGPLPALLAEYFPTQVRYTSLGVVQNAANGWGGGLVPY
VTTWIQTATGNLMWALVYPIALPLLGLIVGLLFLPETARHRLWEQPGAAMATGDGE

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=463, Percent_Identity=33.9092872570194, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1788292, Length=444, Percent_Identity=33.1081081081081, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1789941, Length=416, Percent_Identity=34.1346153846154, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788942, Length=389, Percent_Identity=32.3907455012853, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI87082404, Length=399, Percent_Identity=23.5588972431078, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87082231, Length=440, Percent_Identity=23.1818181818182, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49760; Mature: 49760

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGKGEGTMRAVNNDRRYLAFVIGASTVGSAIEWYDFYIFGSLAAILATHFFAKDHPVAA
CCCCCCCCCEEECCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LLNTIAIFTIGFLFRPLGAIIFGWYGDKIGRKRVFILTLLGMGLGTALIGVIPSYATIGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAPILVFLLRVIQGLSVGGEWGGALTYVAEHAPDGKRGLYTGLLSPAAIYGLIISFFVVL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GVRTALGEEAFRAWGWRIPFLLSLVLVLISLYVRLRLQETPMFAELKARRAVVQNPLKVT
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEE
FTGENLRRVLIAAAVSFGQAVAWYTAIFWALFFLQTAQKLDLITTYKVLLPGLLVAPVLH
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVMGWLSDSIGRKPIVLTGFVLAIVGFYPLYTLLGRFAQKDNLQYWPAALTVMGLMLIAA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
MVWGPLPALLAEYFPTQVRYTSLGVVQNAANGWGGGLVPYVTTWIQTATGNLMWALVYPI
HHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
ALPLLGLIVGLLFLPETARHRLWEQPGAAMATGDGE
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MFGKGEGTMRAVNNDRRYLAFVIGASTVGSAIEWYDFYIFGSLAAILATHFFAKDHPVAA
CCCCCCCCCEEECCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LLNTIAIFTIGFLFRPLGAIIFGWYGDKIGRKRVFILTLLGMGLGTALIGVIPSYATIGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAPILVFLLRVIQGLSVGGEWGGALTYVAEHAPDGKRGLYTGLLSPAAIYGLIISFFVVL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GVRTALGEEAFRAWGWRIPFLLSLVLVLISLYVRLRLQETPMFAELKARRAVVQNPLKVT
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEE
FTGENLRRVLIAAAVSFGQAVAWYTAIFWALFFLQTAQKLDLITTYKVLLPGLLVAPVLH
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVMGWLSDSIGRKPIVLTGFVLAIVGFYPLYTLLGRFAQKDNLQYWPAALTVMGLMLIAA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
MVWGPLPALLAEYFPTQVRYTSLGVVQNAANGWGGGLVPYVTTWIQTATGNLMWALVYPI
HHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
ALPLLGLIVGLLFLPETARHRLWEQPGAAMATGDGE
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]