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The map label for this gene is yvkC [H]

Identifier: 221636186

GI number: 221636186

Start: 762832

End: 764646

Strand: Reverse

Name: yvkC [H]

Synonym: trd_A0780

Alternate gene names: 221636186

Gene position: 764646-762832 (Counterclockwise)

Preceding gene: 221636187

Following gene: 221636185

Centisome position: 83.32

GC content: 61.27

Gene sequence:

>1815_bases
ATGAAGAGCACGTTGCGGTTTCCGAGCCCGTTCGAGGTCGGCACACCACCTGGAGCCGAGGGTTGGGAACAGCTCTATCC
CTACTATCTCCTGTTCAGTGACGATCGCCGGCAAGAGGAAGAGCGTCGTCTCTGGTTCTTCAATGGGATGCACTTCCCTG
AGGCGATGACGCCCTTCGATATCATCACTGCCGAGGCAGGTTACCTCGCGATCGGCGAAATGAGCAGCCGGATGTTCTGC
ATCCCGCCAGCGATGGGGATCGATTTCCGCGTACTGAACGGCTACGTTTACATCAGCCCGACGCCGGTCACTGACCCCGC
GCAGATCGAACAGCGGGCAGTCTGGTTCCAAGAGCGAGCTGGTTACTACTACGCGAATTGGCGAGCGTTCTACGAGGAGT
GGAAGCAGCGACTGAGTGCGGAGGTCGAGCGACTCGTCGCGATCCGCTTCCCGGAACTCCCGGCAGTCGAACCTGCCGAC
TACGTCTTCCAGCGTCGCGGTTACGGTGCGTCGATGGATAATCTCGCGGCTTATCATGACCTGATCGACAGTATCTTCCG
CGTGTGGCAGGTCCACATGGAAATCGTGATGATCGGCTTTGCCGCCTACTTCACGTTTTACGATTTCTGCAAGAAAGCGT
TTCCGGAGATCTCTGACCAGCAGGTGACTCGGATGGTCGGGGCGATCGACGTCAGCATGTTCCGGCCGAACGACGAGCTG
AAGCGGTTGGCCCGTCGGGCGCTCGAACTCGGTATCGCGGATCTTTTCCTCGCGAACGGTTCGGGTGCAGCAGTCTTCGC
GCGGCTGCGCGAGAATGAGGCCGGACGGGCTTGGCTCAGGGAATGGGAGACCAGTGCGGATCCCTGGTTCTACATGAATA
CGGGCGATGGTTTCCAACACCATCACCGGGCGTGGATCGATGATCCGACGCCGGTGGTGAGTGTACTGGCCGACTACATT
CAGCGGCTGCGACGCGGGGAGAATCTCGAGCGAGACACCGCCGCGCTCCGCGAGGAACGCGACCGGATCACCCAGGGCTA
CCGCGAACTCTTGGCGACAGAGGAGGAACGACAAGCCTTCGACCAGATCCTGGCACTGACGCGCGAGGTCTACTACTCGA
TCGAGGACCACAAGTTCTACGTCGAGCACTGGTATCAGAGTCGGTTCTGGAACAAAGTCCGCGAACTCAGCCGGTTGTTC
GTTGCCGGTGGCTTTTTCCGCGACGTGGAAGATATTTTCTTGTTGCACTATACCGAGGTCGAGCGAGCGTTGATGGATCT
CCTGCTCAGCTGGAGCATCGGGACGACGCCGCGTGGTCCGGCCTACTGGCCGGACGAGATCGCCCGGCGCCAACGCATCA
TGGACCGTCTGCGAGCCTGGTCTCCGCCACCAGCACTCGGCGTGGTTCCGGAAGTCATCGGGGATCCAGCGATCATCATG
CTGTGGGGAATCACACCGGAGCGCCTGCGTGCCTGGCTGAGTCCGGAGGAAGGGAACGTCCTCCGTGGGTTCGCTGCCGC
ACCCGGCGTGGCCGAAGGGCCAGCACGGGTCGTGCTGAGCATCGAGGAACTTCAAGAGGTACGGGATGGTGAGATCCTCG
TGTGCTCGGTGACCGAGCCCAGCTGGGCTCCAGTACTGGCCCGCGTTCGTGGGATGGTCTCCGATATCGGGGGCGTGATG
TCGCACGCGGCGATCGTCGCGCGCGAGTACCGGATTCCGGCGGTGCTCGGAACTGGCGTGGCGACCAAAAGGATCCGGAC
AGGGCAGTACCTGCGAGTCGATGGCGAGAACGGAGTCGTCACCATTCTCGACTGA

Upstream 100 bases:

>100_bases
CGTTCGGCATTGTGGAACGACTGTCTTGCGTTCTGCAACACGATTTTCATCATTAGAAATGAAGTCACGCTATATCGCCA
CAACGCGATCGGGGGAGCGG

Downstream 100 bases:

>100_bases
CCAATGGCGAGGTCAGGAAGGAGAACGGTTTCCGATGCGCCGATCACCATTCGTCCGCGAGCTCACTACCCTCGGCCATG
CCGATCGGCCCCTGGTGGGG

Product: hypothetical protein

Products: AMP; phosphoenolpyruvate; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MKSTLRFPSPFEVGTPPGAEGWEQLYPYYLLFSDDRRQEEERRLWFFNGMHFPEAMTPFDIITAEAGYLAIGEMSSRMFC
IPPAMGIDFRVLNGYVYISPTPVTDPAQIEQRAVWFQERAGYYYANWRAFYEEWKQRLSAEVERLVAIRFPELPAVEPAD
YVFQRRGYGASMDNLAAYHDLIDSIFRVWQVHMEIVMIGFAAYFTFYDFCKKAFPEISDQQVTRMVGAIDVSMFRPNDEL
KRLARRALELGIADLFLANGSGAAVFARLRENEAGRAWLREWETSADPWFYMNTGDGFQHHHRAWIDDPTPVVSVLADYI
QRLRRGENLERDTAALREERDRITQGYRELLATEEERQAFDQILALTREVYYSIEDHKFYVEHWYQSRFWNKVRELSRLF
VAGGFFRDVEDIFLLHYTEVERALMDLLLSWSIGTTPRGPAYWPDEIARRQRIMDRLRAWSPPPALGVVPEVIGDPAIIM
LWGITPERLRAWLSPEEGNVLRGFAAAPGVAEGPARVVLSIEELQEVRDGEILVCSVTEPSWAPVLARVRGMVSDIGGVM
SHAAIVAREYRIPAVLGTGVATKRIRTGQYLRVDGENGVVTILD

Sequences:

>Translated_604_residues
MKSTLRFPSPFEVGTPPGAEGWEQLYPYYLLFSDDRRQEEERRLWFFNGMHFPEAMTPFDIITAEAGYLAIGEMSSRMFC
IPPAMGIDFRVLNGYVYISPTPVTDPAQIEQRAVWFQERAGYYYANWRAFYEEWKQRLSAEVERLVAIRFPELPAVEPAD
YVFQRRGYGASMDNLAAYHDLIDSIFRVWQVHMEIVMIGFAAYFTFYDFCKKAFPEISDQQVTRMVGAIDVSMFRPNDEL
KRLARRALELGIADLFLANGSGAAVFARLRENEAGRAWLREWETSADPWFYMNTGDGFQHHHRAWIDDPTPVVSVLADYI
QRLRRGENLERDTAALREERDRITQGYRELLATEEERQAFDQILALTREVYYSIEDHKFYVEHWYQSRFWNKVRELSRLF
VAGGFFRDVEDIFLLHYTEVERALMDLLLSWSIGTTPRGPAYWPDEIARRQRIMDRLRAWSPPPALGVVPEVIGDPAIIM
LWGITPERLRAWLSPEEGNVLRGFAAAPGVAEGPARVVLSIEELQEVRDGEILVCSVTEPSWAPVLARVRGMVSDIGGVM
SHAAIVAREYRIPAVLGTGVATKRIRTGQYLRVDGENGVVTILD
>Mature_604_residues
MKSTLRFPSPFEVGTPPGAEGWEQLYPYYLLFSDDRRQEEERRLWFFNGMHFPEAMTPFDIITAEAGYLAIGEMSSRMFC
IPPAMGIDFRVLNGYVYISPTPVTDPAQIEQRAVWFQERAGYYYANWRAFYEEWKQRLSAEVERLVAIRFPELPAVEPAD
YVFQRRGYGASMDNLAAYHDLIDSIFRVWQVHMEIVMIGFAAYFTFYDFCKKAFPEISDQQVTRMVGAIDVSMFRPNDEL
KRLARRALELGIADLFLANGSGAAVFARLRENEAGRAWLREWETSADPWFYMNTGDGFQHHHRAWIDDPTPVVSVLADYI
QRLRRGENLERDTAALREERDRITQGYRELLATEEERQAFDQILALTREVYYSIEDHKFYVEHWYQSRFWNKVRELSRLF
VAGGFFRDVEDIFLLHYTEVERALMDLLLSWSIGTTPRGPAYWPDEIARRQRIMDRLRAWSPPPALGVVPEVIGDPAIIM
LWGITPERLRAWLSPEEGNVLRGFAAAPGVAEGPARVVLSIEELQEVRDGEILVCSVTEPSWAPVLARVRGMVSDIGGVM
SHAAIVAREYRIPAVLGTGVATKRIRTGQYLRVDGENGVVTILD

Specific function: ESSENTIAL STEP IN GLUCONEOGENESIS WHEN PYRUVATE AND LACTATE ARE USED AS A CARBON SOURCE. [C]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=104, Percent_Identity=36.5384615384615, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17564524, Length=107, Percent_Identity=37.3831775700935, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2

Molecular weight: Translated: 69508; Mature: 69508

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTLRFPSPFEVGTPPGAEGWEQLYPYYLLFSDDRRQEEERRLWFFNGMHFPEAMTPFD
CCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEECCCCCCCHHCCEEEECCCCCCCCCCCHH
IITAEAGYLAIGEMSSRMFCIPPAMGIDFRVLNGYVYISPTPVTDPAQIEQRAVWFQERA
HEECCCCEEEEECCCCCEEEECCCCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHC
GYYYANWRAFYEEWKQRLSAEVERLVAIRFPELPAVEPADYVFQRRGYGASMDNLAAYHD
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHH
LIDSIFRVWQVHMEIVMIGFAAYFTFYDFCKKAFPEISDQQVTRMVGAIDVSMFRPNDEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCHHH
KRLARRALELGIADLFLANGSGAAVFARLRENEAGRAWLREWETSADPWFYMNTGDGFQH
HHHHHHHHHHCHHHEEEECCCCCEEEHHHHCCCCHHHHHHHHCCCCCCEEEEECCCCHHH
HHRAWIDDPTPVVSVLADYIQRLRRGENLERDTAALREERDRITQGYRELLATEEERQAF
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
DQILALTREVYYSIEDHKFYVEHWYQSRFWNKVRELSRLFVAGGFFRDVEDIFLLHYTEV
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ERALMDLLLSWSIGTTPRGPAYWPDEIARRQRIMDRLRAWSPPPALGVVPEVIGDPAIIM
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEE
LWGITPERLRAWLSPEEGNVLRGFAAAPGVAEGPARVVLSIEELQEVRDGEILVCSVTEP
EECCCHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHEEEEHHHHHHCCCCCEEEEEECCC
SWAPVLARVRGMVSDIGGVMSHAAIVAREYRIPAVLGTGVATKRIRTGQYLRVDGENGVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCEEEEECCCCEE
TILD
EEEC
>Mature Secondary Structure
MKSTLRFPSPFEVGTPPGAEGWEQLYPYYLLFSDDRRQEEERRLWFFNGMHFPEAMTPFD
CCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEECCCCCCCHHCCEEEECCCCCCCCCCCHH
IITAEAGYLAIGEMSSRMFCIPPAMGIDFRVLNGYVYISPTPVTDPAQIEQRAVWFQERA
HEECCCCEEEEECCCCCEEEECCCCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHC
GYYYANWRAFYEEWKQRLSAEVERLVAIRFPELPAVEPADYVFQRRGYGASMDNLAAYHD
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHH
LIDSIFRVWQVHMEIVMIGFAAYFTFYDFCKKAFPEISDQQVTRMVGAIDVSMFRPNDEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCHHH
KRLARRALELGIADLFLANGSGAAVFARLRENEAGRAWLREWETSADPWFYMNTGDGFQH
HHHHHHHHHHCHHHEEEECCCCCEEEHHHHCCCCHHHHHHHHCCCCCCEEEEECCCCHHH
HHRAWIDDPTPVVSVLADYIQRLRRGENLERDTAALREERDRITQGYRELLATEEERQAF
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
DQILALTREVYYSIEDHKFYVEHWYQSRFWNKVRELSRLFVAGGFFRDVEDIFLLHYTEV
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ERALMDLLLSWSIGTTPRGPAYWPDEIARRQRIMDRLRAWSPPPALGVVPEVIGDPAIIM
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEE
LWGITPERLRAWLSPEEGNVLRGFAAAPGVAEGPARVVLSIEELQEVRDGEILVCSVTEP
EECCCHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHEEEEHHHHHHCCCCCEEEEEECCC
SWAPVLARVRGMVSDIGGVMSHAAIVAREYRIPAVLGTGVATKRIRTGQYLRVDGENGVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCEEEEECCCCEE
TILD
EEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.083 {pyruvate}} 10.5 {phosphate}} 0.028 {ATP}} [C]

Substrates: ATP; pyruvate; H2O

Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]