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The map label for this gene is dhaAF [H]

Identifier: 221636143

GI number: 221636143

Start: 726810

End: 727688

Strand: Reverse

Name: dhaAF [H]

Synonym: trd_A0736

Alternate gene names: 221636143

Gene position: 727688-726810 (Counterclockwise)

Preceding gene: 221636146

Following gene: 221636137

Centisome position: 79.29

GC content: 66.33

Gene sequence:

>879_bases
ATGGCTGAAGTGCGGACGGCCGACGGAGCTTTCGTTTATGTGGAGGGATGGCGCTGGTTCTTCCGCGAGAGCGGTGAGGG
AGAGCCGGTCGTGCTCCTGCACGGCATCCCGACTTCGAGCTTTTTGTGGCGCAAGGTCCTGCCGGTGCTGGCACGAGAGC
GGCGTGTCATCGCCCCGGATCTTCTCGGGTTCGGTCGCTCGGACAAGCCGCGGCATGGTGCCGAGACGGTGGCGCAACTG
GCGGAGCGTCTGAGTGCCTTGCTGGATCGCTTGCGGGTGGAGCGCTGTGCTCTGGTTGGCCATGACTTTGGCGTGCTCGT
CGCCGCAGCGCTGGTCGAGCGGTGGCCGGATCGGGTCACCCATCTGGTGGTGACCAACACGAGCTTCCGAATCGAGCGGT
GGCGGGGTGGGAGCCTCTCGCCCTTGCAACTCTTGCGGATACCGGTGCTCGGGGAGATCGCCCTGGCGTTGGCCCGACCG
TGGATGCTGGCAGCCGCGCTGCGTCGGTATCTGAACGATCAGAGCGTGCTGGATCGCGCGATGTTGGCTAGGTACTGGGA
ACCGTTCGAGTTGGGATACCGCCGGACGCTCATCCGCTCGATGCGGGTGCCCCCGTTCTCGGCTGACGACCTGGCACGTT
GGCGGGCAACGCTCGTCGAGCGTGGGAGGGGCGGACTCCCGCTTCTGTTGGCCTGGGGGGCGCGCGATCCCCAATTCGGC
GTGGACGAAGCGCGCGAACTGGCCAGTGCTATCCCTGGAGCTCGCTTCCTCAGCTTCCAGCAGGCGAGTCACTTTCTCCC
CGAAGAGCGACCGCGGGCACTCGGTCGCGTGATCGCCGTCTTCCTCGAACGTCCGGCGGCACTTCCTGGTCTGGTCTAG

Upstream 100 bases:

>100_bases
GGAGGGCGCTGGCGTCGTTTCACGGGCTCGCGCGGCGCGGATGGCGGCCTGGTCGAGTGAAGTCGACCGAAGACGGGGTG
CACCGGGTGAGGAACAACGG

Downstream 100 bases:

>100_bases
GACGTCTGCTCGAGCGCACCGATCACTTCGGCAGTGAAGGCGAGCGCATCGATGTAGGCTTCGGTCACGACCGATGCGGC
CAGCGGGAGATCGGCGAGAT

Product: haloalkane dehalogenase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MAEVRTADGAFVYVEGWRWFFRESGEGEPVVLLHGIPTSSFLWRKVLPVLARERRVIAPDLLGFGRSDKPRHGAETVAQL
AERLSALLDRLRVERCALVGHDFGVLVAAALVERWPDRVTHLVVTNTSFRIERWRGGSLSPLQLLRIPVLGEIALALARP
WMLAAALRRYLNDQSVLDRAMLARYWEPFELGYRRTLIRSMRVPPFSADDLARWRATLVERGRGGLPLLLAWGARDPQFG
VDEARELASAIPGARFLSFQQASHFLPEERPRALGRVIAVFLERPAALPGLV

Sequences:

>Translated_292_residues
MAEVRTADGAFVYVEGWRWFFRESGEGEPVVLLHGIPTSSFLWRKVLPVLARERRVIAPDLLGFGRSDKPRHGAETVAQL
AERLSALLDRLRVERCALVGHDFGVLVAAALVERWPDRVTHLVVTNTSFRIERWRGGSLSPLQLLRIPVLGEIALALARP
WMLAAALRRYLNDQSVLDRAMLARYWEPFELGYRRTLIRSMRVPPFSADDLARWRATLVERGRGGLPLLLAWGARDPQFG
VDEARELASAIPGARFLSFQQASHFLPEERPRALGRVIAVFLERPAALPGLV
>Mature_291_residues
AEVRTADGAFVYVEGWRWFFRESGEGEPVVLLHGIPTSSFLWRKVLPVLARERRVIAPDLLGFGRSDKPRHGAETVAQLA
ERLSALLDRLRVERCALVGHDFGVLVAAALVERWPDRVTHLVVTNTSFRIERWRGGSLSPLQLLRIPVLGEIALALARPW
MLAAALRRYLNDQSVLDRAMLARYWEPFELGYRRTLIRSMRVPPFSADDLARWRATLVERGRGGLPLLLAWGARDPQFGV
DEARELASAIPGARFLSFQQASHFLPEERPRALGRVIAVFLERPAALPGLV

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes mono- and di- chlorinated

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI29294637, Length=159, Percent_Identity=37.1069182389937, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI29294635, Length=159, Percent_Identity=37.1069182389937, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI29294639, Length=159, Percent_Identity=37.1069182389937, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI218777837, Length=293, Percent_Identity=25.938566552901, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI27597073, Length=157, Percent_Identity=29.9363057324841, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI32566936, Length=101, Percent_Identity=32.6732673267327, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17558492, Length=101, Percent_Identity=32.6732673267327, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324392, Length=286, Percent_Identity=26.9230769230769, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.5 [H]

Molecular weight: Translated: 32895; Mature: 32763

Theoretical pI: Translated: 11.20; Mature: 11.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVRTADGAFVYVEGWRWFFRESGEGEPVVLLHGIPTSSFLWRKVLPVLARERRVIAPD
CCCCEECCCCEEEEECHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCHH
LLGFGRSDKPRHGAETVAQLAERLSALLDRLRVERCALVGHDFGVLVAAALVERWPDRVT
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHE
HLVVTNTSFRIERWRGGSLSPLQLLRIPVLGEIALALARPWMLAAALRRYLNDQSVLDRA
EEEEECCCEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
MLARYWEPFELGYRRTLIRSMRVPPFSADDLARWRATLVERGRGGLPLLLAWGARDPQFG
HHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
VDEARELASAIPGARFLSFQQASHFLPEERPRALGRVIAVFLERPAALPGLV
HHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AEVRTADGAFVYVEGWRWFFRESGEGEPVVLLHGIPTSSFLWRKVLPVLARERRVIAPD
CCCEECCCCEEEEECHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCHH
LLGFGRSDKPRHGAETVAQLAERLSALLDRLRVERCALVGHDFGVLVAAALVERWPDRVT
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHE
HLVVTNTSFRIERWRGGSLSPLQLLRIPVLGEIALALARPWMLAAALRRYLNDQSVLDRA
EEEEECCCEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
MLARYWEPFELGYRRTLIRSMRVPPFSADDLARWRATLVERGRGGLPLLLAWGARDPQFG
HHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
VDEARELASAIPGARFLSFQQASHFLPEERPRALGRVIAVFLERPAALPGLV
HHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10094681; 10735862 [H]