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The map label for this gene is pmrA [H]

Identifier: 221636097

GI number: 221636097

Start: 679025

End: 680299

Strand: Reverse

Name: pmrA [H]

Synonym: trd_A0691

Alternate gene names: 221636097

Gene position: 680299-679025 (Counterclockwise)

Preceding gene: 221636098

Following gene: 221636096

Centisome position: 74.13

GC content: 69.41

Gene sequence:

>1275_bases
GTGCGACCAGGCGCGAAGGCGCCAGTAGCTGGTGCTGCACTGGGCAGCGCTCCGAACTGGCAGCGAACACTCGGCGCACT
CTGGCTCGCCCAAACGCTGACGATCATCGGGTTCAGCCTCCGGACGCCCTTCTTGCCGTTCTTCGTCGCCGAACTCGGCG
CGAGGAACCTCGAAACGCAGGTCCTCTGGGCCGGAGCGATCAACGCTGGCGGAGCCGCGGTCATGGCGATCACCGCGCCG
CTGTGGGGCGCTCTGGCCGACCGCTACGGGCGCAAGTTGATGGTGCTGCGCGCGATGGTCGCCGGCTCGCTCACGATCGG
TCTGATGGCGCTGGCCACCAGCCCCTGGCAGCTCTTCGTCCTGCGGCTCGTCGAGGGCGGGTTCACCGGCACAGTCACTG
CGTCGACGACGCTGGCTGCCTCGACCGTTCCCAGGGAGCGGCTCGGCTTCGGGCTGGGTCTCATGCAGATGGCAGTCTTC
TCGGGTTCGGCCGTGGGCCCGCTGATCGGCGGGGTACTGGCGGACCAGATCGGCTACCGGGCGACGTTCGTTTTGGCTGG
GAGTCTCCTCCTCGTCTCGGCGCTGCTCGTCGCGTTCCTGGTCGAAGAGCGTTTCGAGCCGCCGCCGCGACGAGCCGGCC
ACGGTGAGACAGGTGGCGAGACCTGGCAGCTGTTGCTGGCGCCGGCGCTGCTCGGAATCGTGGTCAGCCTGTTCGCCTTG
CGGGCAGCTAGCATGGCGCTGCAGCCGATCGTCCCGCTCCTCGTCGCGCAGCTGGCACGCGGTTCCTCGGCGGTCAATTC
CCTCGCCGGGCTGGCGATGGGGGTGAGCGGGATGACCAGCGCGGTCGCATCGGTCGGACTGGGCCGACTGAGCGACCGGA
TCGGCCAGCGACCGTTGCTGATCGCCAGCGGCGCGCTGGCGGCCGCCACCTTCCTCCCGCTGGGGCTGACGACTCAGGTG
TGGCAGGTCATCGTGTTGCAGGCGCTCTTCGGCATCGCCTCCGGCGGCATACTGCCGACCGCGAACGCCCTGGTCGCGCG
GCTTACGCCCGAAGGGCGGCGCGGGACCATTTACGGGTTCACGACAACGGCATCATCGCTGGGAGCCTTCGTCGGTCCGT
TAGTGGGAACAGCGCTCGCGGCCGCGCTCGGGCTTCACGCACCTTTCCTCGTGATCGGTCTTGCGCTGGCGGTGACGGTC
GCGTGGGTCGCGCGGACGCTTTCGCCACTCGAGCGAGCCGAGCGACGAGCTGTCCTCGAGTCCGGGCGCGACTGA

Upstream 100 bases:

>100_bases
CCCCGGTGGACGGTAGCGGACGCGGCTGAAGCGGCGTAGACTGTCCCCTGGCCGACCTGGCAGAGAGTCTGTCGGGTCGG
CGGAGGGGAGTACGGGGACA

Downstream 100 bases:

>100_bases
GGGACGAGCGTCCCGTCGTTCCCACCAGGGAGCCGACTCGGAAACTCCACGCAAGCGACGAGGAGTGACCCACGTCGCCC
ACCCTGTGCTGTCGTGGATC

Product: major facilitator superfamily MFS_1

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MRPGAKAPVAGAALGSAPNWQRTLGALWLAQTLTIIGFSLRTPFLPFFVAELGARNLETQVLWAGAINAGGAAVMAITAP
LWGALADRYGRKLMVLRAMVAGSLTIGLMALATSPWQLFVLRLVEGGFTGTVTASTTLAASTVPRERLGFGLGLMQMAVF
SGSAVGPLIGGVLADQIGYRATFVLAGSLLLVSALLVAFLVEERFEPPPRRAGHGETGGETWQLLLAPALLGIVVSLFAL
RAASMALQPIVPLLVAQLARGSSAVNSLAGLAMGVSGMTSAVASVGLGRLSDRIGQRPLLIASGALAAATFLPLGLTTQV
WQVIVLQALFGIASGGILPTANALVARLTPEGRRGTIYGFTTTASSLGAFVGPLVGTALAAALGLHAPFLVIGLALAVTV
AWVARTLSPLERAERRAVLESGRD

Sequences:

>Translated_424_residues
MRPGAKAPVAGAALGSAPNWQRTLGALWLAQTLTIIGFSLRTPFLPFFVAELGARNLETQVLWAGAINAGGAAVMAITAP
LWGALADRYGRKLMVLRAMVAGSLTIGLMALATSPWQLFVLRLVEGGFTGTVTASTTLAASTVPRERLGFGLGLMQMAVF
SGSAVGPLIGGVLADQIGYRATFVLAGSLLLVSALLVAFLVEERFEPPPRRAGHGETGGETWQLLLAPALLGIVVSLFAL
RAASMALQPIVPLLVAQLARGSSAVNSLAGLAMGVSGMTSAVASVGLGRLSDRIGQRPLLIASGALAAATFLPLGLTTQV
WQVIVLQALFGIASGGILPTANALVARLTPEGRRGTIYGFTTTASSLGAFVGPLVGTALAAALGLHAPFLVIGLALAVTV
AWVARTLSPLERAERRAVLESGRD
>Mature_424_residues
MRPGAKAPVAGAALGSAPNWQRTLGALWLAQTLTIIGFSLRTPFLPFFVAELGARNLETQVLWAGAINAGGAAVMAITAP
LWGALADRYGRKLMVLRAMVAGSLTIGLMALATSPWQLFVLRLVEGGFTGTVTASTTLAASTVPRERLGFGLGLMQMAVF
SGSAVGPLIGGVLADQIGYRATFVLAGSLLLVSALLVAFLVEERFEPPPRRAGHGETGGETWQLLLAPALLGIVVSLFAL
RAASMALQPIVPLLVAQLARGSSAVNSLAGLAMGVSGMTSAVASVGLGRLSDRIGQRPLLIASGALAAATFLPLGLTTQV
WQVIVLQALFGIASGGILPTANALVARLTPEGRRGTIYGFTTTASSLGAFVGPLVGTALAAALGLHAPFLVIGLALAVTV
AWVARTLSPLERAERRAVLESGRD

Specific function: Efflux pump for various substrates [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=375, Percent_Identity=33.3333333333333, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87081738, Length=159, Percent_Identity=32.0754716981132, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082404, Length=396, Percent_Identity=22.4747474747475, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25146993, Length=388, Percent_Identity=24.2268041237113, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193210343, Length=388, Percent_Identity=24.2268041237113, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43467; Mature: 43467

Theoretical pI: Translated: 11.97; Mature: 11.97

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPGAKAPVAGAALGSAPNWQRTLGALWLAQTLTIIGFSLRTPFLPFFVAELGARNLETQ
CCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEE
VLWAGAINAGGAAVMAITAPLWGALADRYGRKLMVLRAMVAGSLTIGLMALATSPWQLFV
EEEEECCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LRLVEGGFTGTVTASTTLAASTVPRERLGFGLGLMQMAVFSGSAVGPLIGGVLADQIGYR
HHHHHCCCCEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHH
ATFVLAGSLLLVSALLVAFLVEERFEPPPRRAGHGETGGETWQLLLAPALLGIVVSLFAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RAASMALQPIVPLLVAQLARGSSAVNSLAGLAMGVSGMTSAVASVGLGRLSDRIGQRPLL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IASGALAAATFLPLGLTTQVWQVIVLQALFGIASGGILPTANALVARLTPEGRRGTIYGF
EECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEEE
TTTASSLGAFVGPLVGTALAAALGLHAPFLVIGLALAVTVAWVARTLSPLERAERRAVLE
ECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGRD
CCCC
>Mature Secondary Structure
MRPGAKAPVAGAALGSAPNWQRTLGALWLAQTLTIIGFSLRTPFLPFFVAELGARNLETQ
CCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEE
VLWAGAINAGGAAVMAITAPLWGALADRYGRKLMVLRAMVAGSLTIGLMALATSPWQLFV
EEEEECCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LRLVEGGFTGTVTASTTLAASTVPRERLGFGLGLMQMAVFSGSAVGPLIGGVLADQIGYR
HHHHHCCCCEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHH
ATFVLAGSLLLVSALLVAFLVEERFEPPPRRAGHGETGGETWQLLLAPALLGIVVSLFAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RAASMALQPIVPLLVAQLARGSSAVNSLAGLAMGVSGMTSAVASVGLGRLSDRIGQRPLL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IASGALAAATFLPLGLTTQVWQVIVLQALFGIASGGILPTANALVARLTPEGRRGTIYGF
EECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEEE
TTTASSLGAFVGPLVGTALAAALGLHAPFLVIGLALAVTVAWVARTLSPLERAERRAVLE
ECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGRD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]