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The map label for this gene is mdeA [H]

Identifier: 221636081

GI number: 221636081

Start: 666240

End: 667433

Strand: Reverse

Name: mdeA [H]

Synonym: trd_A0675

Alternate gene names: 221636081

Gene position: 667433-666240 (Counterclockwise)

Preceding gene: 221636083

Following gene: 221636074

Centisome position: 72.73

GC content: 66.58

Gene sequence:

>1194_bases
ATGCCCGAGCAGAAACCGGCGACCAGGCCAGAAACGATCTGCACGCAGGCAGGACTGCACCAGCTGGAGAGCCGAACAGT
ACCGCTCGCTCCACCGATCTGGCAGACGAGCGCTTTTGCCTATCCGGATATGGAGGCGATGGACGAGATCGCCCGCGGTG
AGGAGCCGGGGTTCATCTATGCCCGCTACGGGTTGCCGAACCACCGGCAGCTCGAGCTGGCACTGGCGAGCCTGGAAGGG
GCCGAAGACGCGGTCGTCACCGCGAGCGGCATGAGCGCGATCGCCGCCACGCTCTTCGCGCTCACCATACCCGGCGAGCG
AGTAGTCGCCGACACGCACGTCTATCGCGGTACGAGACTGCTCTTGGAGCGAGACCTCGCGCGCTTTGGGGTGATCGTCG
ACTTCGTCGATTGTGGTGATCTCGAGGCGGTGGCAGGTGCACTGGAGCGTCCGGCTCGACTGCTGTGGGTGGACACGCTG
ACCAACCCGACCCTCCGGGTGCGCAATCTTCCGAGACTGGCGGCGCTCGCCCGAGCTGCCGGGGCACTGATGGTCGTGGA
CAACACGTTCGCGACGCCGGTGCATTGCCGGCCGTTGTCCTGGGGAGCGGTGCTGGTCGTGCATTCCACCACCAAGTTGA
TCGGTGGGCATCACGATGCGAGTGGCGGCGTCGTTGCCGGACCAGCGGCCGTGGTCGAGACGATCCGAGGACAGGTGGTG
CGCTTCGGTGGGCGAGGTGGAGCGTTCGACAGCTGGTTGATCCAGCGTGGTCTGGTCACGCTGCCCCTGCGGGCAGCACA
GAGCAGTCAGAGCGCGCTCCTCCTGGCGGAACGCCTGGCCGCTCATCCGATGGTGCAGGCGGTGCTCTATCCGGGCCTGC
CGGGTCATCCGGAACACCGGCTGGCTGAACGACTGTTGCGTGACGGCTTTGGGGCCGTGGTCGCTTTCGAGTTCGCTGGT
GGAGAGCCGGCGGTGCGGCGATTCGTGCGTGCTCTCCGCTTGGTGCGCTTCGCTGAAACGCTGGGTGGTGCCGTGACGAC
ACTCGTGCACCCGGCGACCACCTCGCACCGTTCGCTGCCCGTGAGCGAGCGTGAGCGACTGGGGATCAGCGATGGGCTCC
TGCGGCTCTCGGTCGGACTGGAACACCCGGAGGAACTCTGGCAGGACCTGGAACAGGCACTGGGGCACATCTGA

Upstream 100 bases:

>100_bases
CGGCTCATCGCGTTCCCTCGCTTTCCGTGTCACGTCCACTCTTCGAGCGGAAGTATGCTGCGCTCGGGTGGTCGGAAACG
AGAGCGAGAGGAGTCCGGCG

Downstream 100 bases:

>100_bases
CGGTTCACGGCTCCTCAGCGAGCCGACGCGCGAGTTCTCGCCAATCGTCGAGCGCTGCTGCGCGGTCGTGCGGATCCTCA
TGCTCCGCGTAGCGAGCGAC

Product: methionine gamma-lyase (L-methioninase)

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MPEQKPATRPETICTQAGLHQLESRTVPLAPPIWQTSAFAYPDMEAMDEIARGEEPGFIYARYGLPNHRQLELALASLEG
AEDAVVTASGMSAIAATLFALTIPGERVVADTHVYRGTRLLLERDLARFGVIVDFVDCGDLEAVAGALERPARLLWVDTL
TNPTLRVRNLPRLAALARAAGALMVVDNTFATPVHCRPLSWGAVLVVHSTTKLIGGHHDASGGVVAGPAAVVETIRGQVV
RFGGRGGAFDSWLIQRGLVTLPLRAAQSSQSALLLAERLAAHPMVQAVLYPGLPGHPEHRLAERLLRDGFGAVVAFEFAG
GEPAVRRFVRALRLVRFAETLGGAVTTLVHPATTSHRSLPVSERERLGISDGLLRLSVGLEHPEELWQDLEQALGHI

Sequences:

>Translated_397_residues
MPEQKPATRPETICTQAGLHQLESRTVPLAPPIWQTSAFAYPDMEAMDEIARGEEPGFIYARYGLPNHRQLELALASLEG
AEDAVVTASGMSAIAATLFALTIPGERVVADTHVYRGTRLLLERDLARFGVIVDFVDCGDLEAVAGALERPARLLWVDTL
TNPTLRVRNLPRLAALARAAGALMVVDNTFATPVHCRPLSWGAVLVVHSTTKLIGGHHDASGGVVAGPAAVVETIRGQVV
RFGGRGGAFDSWLIQRGLVTLPLRAAQSSQSALLLAERLAAHPMVQAVLYPGLPGHPEHRLAERLLRDGFGAVVAFEFAG
GEPAVRRFVRALRLVRFAETLGGAVTTLVHPATTSHRSLPVSERERLGISDGLLRLSVGLEHPEELWQDLEQALGHI
>Mature_396_residues
PEQKPATRPETICTQAGLHQLESRTVPLAPPIWQTSAFAYPDMEAMDEIARGEEPGFIYARYGLPNHRQLELALASLEGA
EDAVVTASGMSAIAATLFALTIPGERVVADTHVYRGTRLLLERDLARFGVIVDFVDCGDLEAVAGALERPARLLWVDTLT
NPTLRVRNLPRLAALARAAGALMVVDNTFATPVHCRPLSWGAVLVVHSTTKLIGGHHDASGGVVAGPAAVVETIRGQVVR
FGGRGGAFDSWLIQRGLVTLPLRAAQSSQSALLLAERLAAHPMVQAVLYPGLPGHPEHRLAERLLRDGFGAVVAFEFAGG
EPAVRRFVRALRLVRFAETLGGAVTTLVHPATTSHRSLPVSERERLGISDGLLRLSVGLEHPEELWQDLEQALGHI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=361, Percent_Identity=34.3490304709141, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI299473759, Length=361, Percent_Identity=31.5789473684211, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI25453487, Length=360, Percent_Identity=30.2777777777778, Blast_Score=151, Evalue=9e-37,
Organism=Escherichia coli, GI1790375, Length=387, Percent_Identity=37.2093023255814, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789383, Length=372, Percent_Identity=25.8064516129032, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71980708, Length=376, Percent_Identity=31.9148936170213, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17560056, Length=374, Percent_Identity=34.4919786096257, Blast_Score=201, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI17538055, Length=370, Percent_Identity=34.0540540540541, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=346, Percent_Identity=37.8612716763006, Blast_Score=199, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=410, Percent_Identity=28.0487804878049, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321254, Length=414, Percent_Identity=26.0869565217391, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=317, Percent_Identity=26.813880126183, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=390, Percent_Identity=26.1538461538462, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922554, Length=373, Percent_Identity=33.7801608579088, Blast_Score=217, Evalue=9e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 42705; Mature: 42573

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEQKPATRPETICTQAGLHQLESRTVPLAPPIWQTSAFAYPDMEAMDEIARGEEPGFIY
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEE
ARYGLPNHRQLELALASLEGAEDAVVTASGMSAIAATLFALTIPGERVVADTHVYRGTRL
EECCCCCCCCCEEEEECCCCCCCEEEEECCHHHHHHHHHHEECCCCEEEECHHHHHHHHH
LLERDLARFGVIVDFVDCGDLEAVAGALERPARLLWVDTLTNPTLRVRNLPRLAALARAA
HHHHHHHHHHHEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCEEECCCHHHHHHHHHC
GALMVVDNTFATPVHCRPLSWGAVLVVHSTTKLIGGHHDASGGVVAGPAAVVETIRGQVV
CEEEEEECCCCCCEEEEECCCCEEEEEECHHHHHCCCCCCCCCEEECHHHHHHHHCCCEE
RFGGRGGAFDSWLIQRGLVTLPLRAAQSSQSALLLAERLAAHPMVQAVLYPGLPGHPEHR
EECCCCCCHHHHHHHCCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LAERLLRDGFGAVVAFEFAGGEPAVRRFVRALRLVRFAETLGGAVTTLVHPATTSHRSLP
HHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCC
VSERERLGISDGLLRLSVGLEHPEELWQDLEQALGHI
CCHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PEQKPATRPETICTQAGLHQLESRTVPLAPPIWQTSAFAYPDMEAMDEIARGEEPGFIY
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEE
ARYGLPNHRQLELALASLEGAEDAVVTASGMSAIAATLFALTIPGERVVADTHVYRGTRL
EECCCCCCCCCEEEEECCCCCCCEEEEECCHHHHHHHHHHEECCCCEEEECHHHHHHHHH
LLERDLARFGVIVDFVDCGDLEAVAGALERPARLLWVDTLTNPTLRVRNLPRLAALARAA
HHHHHHHHHHHEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCEEECCCHHHHHHHHHC
GALMVVDNTFATPVHCRPLSWGAVLVVHSTTKLIGGHHDASGGVVAGPAAVVETIRGQVV
CEEEEEECCCCCCEEEEECCCCEEEEEECHHHHHCCCCCCCCCEEECHHHHHHHHCCCEE
RFGGRGGAFDSWLIQRGLVTLPLRAAQSSQSALLLAERLAAHPMVQAVLYPGLPGHPEHR
EECCCCCCHHHHHHHCCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LAERLLRDGFGAVVAFEFAGGEPAVRRFVRALRLVRFAETLGGAVTTLVHPATTSHRSLP
HHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCC
VSERERLGISDGLLRLSVGLEHPEELWQDLEQALGHI
CCHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]