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The map label for this gene is glnA [H]

Identifier: 221635836

GI number: 221635836

Start: 416018

End: 417403

Strand: Reverse

Name: glnA [H]

Synonym: trd_A0430

Alternate gene names: 221635836

Gene position: 417403-416018 (Counterclockwise)

Preceding gene: 221635840

Following gene: 221635835

Centisome position: 45.48

GC content: 64.79

Gene sequence:

>1386_bases
ATGAGCGAGCATACGACGGGTCTCTTGACCCTGGACGAACTGGAGCACCTGGTCGCCAGTGGCGAGATCGATACGGTCAT
CAACGCGCTTTGCGACATGCAGGGACGCCTCATGGGGAAGCGCGTCCGCGCCGATTTCTTCCTCGAGCACGTCCGCGCCC
ACGGCACCCACTTCTGCACCTACCTCCTCGGTACCGACATGGAGATGACCACACCGGGCGGTTACCGGTTGATGAATTGG
GAGACCGGCTACGGTGACTGGGTCGCCCGTCCCGACTTCGCCACGCTGCGGCGTATCCCCTGGCTGGAGGGAACCGCGCT
CGTCTTGGCCGACGCGGTCGACGAGGAAACCGGTGAGCTGATCCCGGTCGCCCCCCGGACGATCCTCCGCCGCCAGGTCG
AGCGCGCCGCAGCCATGGGCTTCCGCATCATGATGGCGAGCGAGCTGGAGTTCTACCTCCTCAAGGACTCCTATGACGAG
GCGTACGAGAAGGGGTACCGTGGTCTGCACCCCTACGGCTGGTACAACGAGGACTATCACCTCTTCCAGGGAACCAAAGC
GGAACCGATCTACCGGCAGATCCGCAACCTGATGACGGCTGCTGGGATCCCGATCGAGTTCTCGAAGGGGGAGGCAGCGG
CTGGCCAGCACGAGATCAACATCCACTACGCGGAGGCGCTCGAATCGGCCGATCGTGCGGTGCTCTTCAAGCACGGCGTC
AAGGAGATCGCCTACCTCAATGGGGTGGCAGTCACCTTCATGGCCAAGCCCCATCACACCTGGACCGGCTCCAGTTCCCA
CATCCACTTGAGCATCTGGGACAGCGCGGGCGAGCGGAACCTCTTCTACGAGGCAGACGGGCAACCCTACCACATGAGTA
AGCTGATGCAGCACGCACTCGCCGGACTCATCGCCCACGCCCGGGAACTTTCGCTCTTCGTCGCCTCCAACGTCAACTCG
TACAAGCGCTACGCCGTCGCTAGCTGGGCACCGGTCAACGTCGTCTGGGGACGCGACAACCGCACCTGTGGCTTTCGCAT
CGTCGGCCACGGCCAGTCATTGCGCGTCGAGAGCCGCCTTCCGGGCGCCGACGCCAACCCGTACCTGGCTTATGCCGCGA
TGATCGCCGCCGCGCTGGACGGGATCGAGCGAGAACTTCCCCTGCCGCCCGAGTACCGCGGCAACGCCTACGAAGCCCGC
GACGTCGTCCGTGTTCCGCGCACCCTCGCCGAGGCGATGGTCGAGTTCGAGCGCAGCGAACTGGCCCGCCGCGCTTTCGG
CGAGCTCGTCGTCGAGCACTACGTCAACATGGCGCGGGTCGAGCAGGAGACGTTCGACGCCGTGGTGACCGATTGGGAGC
TGTACCGGTACTTCGAGCGCGGCTGA

Upstream 100 bases:

>100_bases
CAGTGCTGCTGGTTAGCCGGCGGTAGCCGTCACCCCCCGCCCGCTGACTCACCTCCGGCCGATCGGTGAGGCAACGAGCG
AAGGGAGTTGGAGCGCATCG

Downstream 100 bases:

>100_bases
GGGAGGGGGTAACGATGGGCAACCGGCTGGCTGGCAAAGTGTGCATCATCACTGGAGCAGGATCGGGAATCGGGCGCGAG
GCGGCCATCCTCTTCGCCCA

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSEHTTGLLTLDELEHLVASGEIDTVINALCDMQGRLMGKRVRADFFLEHVRAHGTHFCTYLLGTDMEMTTPGGYRLMNW
ETGYGDWVARPDFATLRRIPWLEGTALVLADAVDEETGELIPVAPRTILRRQVERAAAMGFRIMMASELEFYLLKDSYDE
AYEKGYRGLHPYGWYNEDYHLFQGTKAEPIYRQIRNLMTAAGIPIEFSKGEAAAGQHEINIHYAEALESADRAVLFKHGV
KEIAYLNGVAVTFMAKPHHTWTGSSSHIHLSIWDSAGERNLFYEADGQPYHMSKLMQHALAGLIAHARELSLFVASNVNS
YKRYAVASWAPVNVVWGRDNRTCGFRIVGHGQSLRVESRLPGADANPYLAYAAMIAAALDGIERELPLPPEYRGNAYEAR
DVVRVPRTLAEAMVEFERSELARRAFGELVVEHYVNMARVEQETFDAVVTDWELYRYFERG

Sequences:

>Translated_461_residues
MSEHTTGLLTLDELEHLVASGEIDTVINALCDMQGRLMGKRVRADFFLEHVRAHGTHFCTYLLGTDMEMTTPGGYRLMNW
ETGYGDWVARPDFATLRRIPWLEGTALVLADAVDEETGELIPVAPRTILRRQVERAAAMGFRIMMASELEFYLLKDSYDE
AYEKGYRGLHPYGWYNEDYHLFQGTKAEPIYRQIRNLMTAAGIPIEFSKGEAAAGQHEINIHYAEALESADRAVLFKHGV
KEIAYLNGVAVTFMAKPHHTWTGSSSHIHLSIWDSAGERNLFYEADGQPYHMSKLMQHALAGLIAHARELSLFVASNVNS
YKRYAVASWAPVNVVWGRDNRTCGFRIVGHGQSLRVESRLPGADANPYLAYAAMIAAALDGIERELPLPPEYRGNAYEAR
DVVRVPRTLAEAMVEFERSELARRAFGELVVEHYVNMARVEQETFDAVVTDWELYRYFERG
>Mature_460_residues
SEHTTGLLTLDELEHLVASGEIDTVINALCDMQGRLMGKRVRADFFLEHVRAHGTHFCTYLLGTDMEMTTPGGYRLMNWE
TGYGDWVARPDFATLRRIPWLEGTALVLADAVDEETGELIPVAPRTILRRQVERAAAMGFRIMMASELEFYLLKDSYDEA
YEKGYRGLHPYGWYNEDYHLFQGTKAEPIYRQIRNLMTAAGIPIEFSKGEAAAGQHEINIHYAEALESADRAVLFKHGVK
EIAYLNGVAVTFMAKPHHTWTGSSSHIHLSIWDSAGERNLFYEADGQPYHMSKLMQHALAGLIAHARELSLFVASNVNSY
KRYAVASWAPVNVVWGRDNRTCGFRIVGHGQSLRVESRLPGADANPYLAYAAMIAAALDGIERELPLPPEYRGNAYEARD
VVRVPRTLAEAMVEFERSELARRAFGELVVEHYVNMARVEQETFDAVVTDWELYRYFERG

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=435, Percent_Identity=27.3563218390805, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI87081870, Length=439, Percent_Identity=31.4350797266515, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1790301, Length=437, Percent_Identity=26.3157894736842, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52177; Mature: 52046

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHTTGLLTLDELEHLVASGEIDTVINALCDMQGRLMGKRVRADFFLEHVRAHGTHFCT
CCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
YLLGTDMEMTTPGGYRLMNWETGYGDWVARPDFATLRRIPWLEGTALVLADAVDEETGEL
HHHCCCCEEECCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCE
IPVAPRTILRRQVERAAAMGFRIMMASELEFYLLKDSYDEAYEKGYRGLHPYGWYNEDYH
EECCHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCEE
LFQGTKAEPIYRQIRNLMTAAGIPIEFSKGEAAAGQHEINIHYAEALESADRAVLFKHGV
EECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEHHHHHCCCCHHHHHHHHH
KEIAYLNGVAVTFMAKPHHTWTGSSSHIHLSIWDSAGERNLFYEADGQPYHMSKLMQHAL
HHHHHHCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHH
AGLIAHARELSLFVASNVNSYKRYAVASWAPVNVVWGRDNRTCGFRIVGHGQSLRVESRL
HHHHHHHHHHEEEEECCCCHHHHEEEECCCCEEEEECCCCCCEEEEEEECCCEEEECCCC
PGADANPYLAYAAMIAAALDGIERELPLPPEYRGNAYEARDVVRVPRTLAEAMVEFERSE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LARRAFGELVVEHYVNMARVEQETFDAVVTDWELYRYFERG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEHTTGLLTLDELEHLVASGEIDTVINALCDMQGRLMGKRVRADFFLEHVRAHGTHFCT
CCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
YLLGTDMEMTTPGGYRLMNWETGYGDWVARPDFATLRRIPWLEGTALVLADAVDEETGEL
HHHCCCCEEECCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCE
IPVAPRTILRRQVERAAAMGFRIMMASELEFYLLKDSYDEAYEKGYRGLHPYGWYNEDYH
EECCHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCEE
LFQGTKAEPIYRQIRNLMTAAGIPIEFSKGEAAAGQHEINIHYAEALESADRAVLFKHGV
EECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEHHHHHCCCCHHHHHHHHH
KEIAYLNGVAVTFMAKPHHTWTGSSSHIHLSIWDSAGERNLFYEADGQPYHMSKLMQHAL
HHHHHHCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHH
AGLIAHARELSLFVASNVNSYKRYAVASWAPVNVVWGRDNRTCGFRIVGHGQSLRVESRL
HHHHHHHHHHEEEEECCCCHHHHEEEECCCCEEEEECCCCCCEEEEEEECCCEEEECCCC
PGADANPYLAYAAMIAAALDGIERELPLPPEYRGNAYEARDVVRVPRTLAEAMVEFERSE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LARRAFGELVVEHYVNMARVEQETFDAVVTDWELYRYFERG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]