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The map label for this gene is mrpD [H]

Identifier: 221635778

GI number: 221635778

Start: 357776

End: 359293

Strand: Reverse

Name: mrpD [H]

Synonym: trd_A0372

Alternate gene names: 221635778

Gene position: 359293-357776 (Counterclockwise)

Preceding gene: 221635779

Following gene: 221635777

Centisome position: 39.15

GC content: 62.91

Gene sequence:

>1518_bases
ATGGGTCTCCTCCTGAGTCTCCCGCTCCTGCTACCGCTCGGCTCGAGCCTCCTCCTCTTCGTGACTCGCCACAACCGTCG
AGCGAGCCACTTCGTGGCTATCACGAGCGCAACCGTTCTCCTCGTGCTCGTCAGCGGGTTGACCCTGCAGGTGGCGCGGG
ATGGACTCCTCGTCGTCGCGATCGGTTCTTGGCCACCGCCCTACGGAATCGTCTTGGTGGCCGATTTGCTCAGCGTACTC
ATGCTGCTCGTCTGCACGCTCGTCGCCTGGGCGACCGTGCTCTATTCCACCGCCTGGATCGATCCCGCCCGCGAGCGCCT
CGGGTACTATCCGCTCATTTTCGCCTTGCTCTTCGGCGTCAACGGCACGTTCCTCACTGGCGACATCTTCAACTTGTACG
TCTGGTTCGAGATCCTGCTCATGTCCTCGTTCGTGTTGCTCGTCCTCGGTGCCGAGCGAGGGCAGGTCGAAGGTGGACTC
AAGTACGTCGCCTTGAACCTGCTCGCCTCCGCCTTTTTCCTGAGTGCGGTCGGCGTCCTCTACGGCGTGACTGGCACGCT
CAACTTCGCCCACCTGAGCCGAGCGGTACTCGGCATCGACCCGCTCGTGCTGGATCTTCTCGCTGTGCTCTTCGGCATCG
CCTTCGGCATCAAGGCCGCGGTCTTCCCGCTCTTCTTCTGGCTCCCTGCTTCCTATCACACGCCACCGGTGCCGGTCTCC
GCACTCTTCGCTGGTCTCCTGACCAAGGTCGGTGTGTACTCGCTCATTCGCATCTTCACGCTCCTCTTCGTTCACGATCC
GGGTTGGTCGCATCGTCTCCTCTTGGTCTGCGCCGCCCTGACCATGCTGACCGGTGTGCTGGGCGCATTGGCCCAGCAGG
ATGTCCGTCGCATCCTCTCCTTTCATATCGTCAGCCAGATCGGCTACATGATCTTCGGCCTTGCGCTGCTGACCCCGCTC
GGTCTCGCCAGCAGCGTCTTCTATCTGGTCCACCACATCGTGGTGAAGACGAACCTGTTTCTCATCGGCGGCCTGATCGA
ACGACTCGGCGGGAGCAGCCGGCTCAGCGACCTCGGCGCCCTCTACCGTGCTCGTCCCTGGCTCGGACTCCTCTTTTTAG
TTCCCGCGTTCTCCTTGGCCGGTCTTCCACCGCTCTCCGGCTTCCTGGCCAAGCTGCTCGTCATCCGCGCTGGACTGGCC
GCGGGTGCCTACTGGGCGACTGCCATCGCCGTCATCGTGAGCTTGTTCACTCTTCTCTCCATGCTCAAGATCTGGAACGA
GGCTTTCTGGAAACCGCGACCGGTCGAGGATCCGCCTCGCCAGCCACTCTCAACCCTCCTCCTGCTGCCAGTCCTCGTGC
TCGCGGCAGTGACGGTCCTGCTCGGCACCGCCAGTGAGCCGCTCCTGGTTTTGGCGACTCGCGCAGCAGACCAACTGCTC
GATCCTCAGATCTACCAGCGAGCCGTGCTCGGCCAGCTCGGATCACCAGCACTCGCATCGATCGGAGGTGCACCGTGA

Upstream 100 bases:

>100_bases
TGACGGCGACTGACCGGCTCGGTGAGGTACCGGCGCCCGCTCATCGCCCGATCACGGCAGACGAGGAGGACGGCTTGGTT
CGGGGAGAGGAGGAGCGCGC

Downstream 100 bases:

>100_bases
CGCTGTTCCTGCTCAATCTTCTGCTCGCCCTCGTCTGGGCTATGGCAGTCGGCCGCTTCACCGTTCCCCAACTGCTACTC
GGCTACGTGCTCGGATTTCT

Product: Na(+)/H(+) antiporter subunit D (Multiple resistance and pH homeostasis protein D) (Mrp complex subunit D)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MGLLLSLPLLLPLGSSLLLFVTRHNRRASHFVAITSATVLLVLVSGLTLQVARDGLLVVAIGSWPPPYGIVLVADLLSVL
MLLVCTLVAWATVLYSTAWIDPARERLGYYPLIFALLFGVNGTFLTGDIFNLYVWFEILLMSSFVLLVLGAERGQVEGGL
KYVALNLLASAFFLSAVGVLYGVTGTLNFAHLSRAVLGIDPLVLDLLAVLFGIAFGIKAAVFPLFFWLPASYHTPPVPVS
ALFAGLLTKVGVYSLIRIFTLLFVHDPGWSHRLLLVCAALTMLTGVLGALAQQDVRRILSFHIVSQIGYMIFGLALLTPL
GLASSVFYLVHHIVVKTNLFLIGGLIERLGGSSRLSDLGALYRARPWLGLLFLVPAFSLAGLPPLSGFLAKLLVIRAGLA
AGAYWATAIAVIVSLFTLLSMLKIWNEAFWKPRPVEDPPRQPLSTLLLLPVLVLAAVTVLLGTASEPLLVLATRAADQLL
DPQIYQRAVLGQLGSPALASIGGAP

Sequences:

>Translated_505_residues
MGLLLSLPLLLPLGSSLLLFVTRHNRRASHFVAITSATVLLVLVSGLTLQVARDGLLVVAIGSWPPPYGIVLVADLLSVL
MLLVCTLVAWATVLYSTAWIDPARERLGYYPLIFALLFGVNGTFLTGDIFNLYVWFEILLMSSFVLLVLGAERGQVEGGL
KYVALNLLASAFFLSAVGVLYGVTGTLNFAHLSRAVLGIDPLVLDLLAVLFGIAFGIKAAVFPLFFWLPASYHTPPVPVS
ALFAGLLTKVGVYSLIRIFTLLFVHDPGWSHRLLLVCAALTMLTGVLGALAQQDVRRILSFHIVSQIGYMIFGLALLTPL
GLASSVFYLVHHIVVKTNLFLIGGLIERLGGSSRLSDLGALYRARPWLGLLFLVPAFSLAGLPPLSGFLAKLLVIRAGLA
AGAYWATAIAVIVSLFTLLSMLKIWNEAFWKPRPVEDPPRQPLSTLLLLPVLVLAAVTVLLGTASEPLLVLATRAADQLL
DPQIYQRAVLGQLGSPALASIGGAP
>Mature_504_residues
GLLLSLPLLLPLGSSLLLFVTRHNRRASHFVAITSATVLLVLVSGLTLQVARDGLLVVAIGSWPPPYGIVLVADLLSVLM
LLVCTLVAWATVLYSTAWIDPARERLGYYPLIFALLFGVNGTFLTGDIFNLYVWFEILLMSSFVLLVLGAERGQVEGGLK
YVALNLLASAFFLSAVGVLYGVTGTLNFAHLSRAVLGIDPLVLDLLAVLFGIAFGIKAAVFPLFFWLPASYHTPPVPVSA
LFAGLLTKVGVYSLIRIFTLLFVHDPGWSHRLLLVCAALTMLTGVLGALAQQDVRRILSFHIVSQIGYMIFGLALLTPLG
LASSVFYLVHHIVVKTNLFLIGGLIERLGGSSRLSDLGALYRARPWLGLLFLVPAFSLAGLPPLSGFLAKLLVIRAGLAA
GAYWATAIAVIVSLFTLLSMLKIWNEAFWKPRPVEDPPRQPLSTLLLLPVLVLAAVTVLLGTASEPLLVLATRAADQLLD
PQIYQRAVLGQLGSPALASIGGAP

Specific function: Mrp complex is a Na(+)/H(+) antiporter involved in Na(+) excretion and Na(+) resistance [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=405, Percent_Identity=23.9506172839506, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1788827, Length=441, Percent_Identity=26.0770975056689, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1788829, Length=348, Percent_Identity=30.4597701149425, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1788831, Length=405, Percent_Identity=25.4320987654321, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788613, Length=373, Percent_Identity=26.0053619302949, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI145693160, Length=332, Percent_Identity=26.8072289156627, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788614, Length=348, Percent_Identity=28.735632183908, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI2367154, Length=292, Percent_Identity=27.7397260273973, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 54152; Mature: 54021

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLLSLPLLLPLGSSLLLFVTRHNRRASHFVAITSATVLLVLVSGLTLQVARDGLLVVA
CCHHHHHHHHHHCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHEEECCCEEEEE
IGSWPPPYGIVLVADLLSVLMLLVCTLVAWATVLYSTAWIDPARERLGYYPLIFALLFGV
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHCC
NGTFLTGDIFNLYVWFEILLMSSFVLLVLGAERGQVEGGLKYVALNLLASAFFLSAVGVL
CCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YGVTGTLNFAHLSRAVLGIDPLVLDLLAVLFGIAFGIKAAVFPLFFWLPASYHTPPVPVS
HHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
ALFAGLLTKVGVYSLIRIFTLLFVHDPGWSHRLLLVCAALTMLTGVLGALAQQDVRRILS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHIVSQIGYMIFGLALLTPLGLASSVFYLVHHIVVKTNLFLIGGLIERLGGSSRLSDLGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LYRARPWLGLLFLVPAFSLAGLPPLSGFLAKLLVIRAGLAAGAYWATAIAVIVSLFTLLS
HHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLKIWNEAFWKPRPVEDPPRQPLSTLLLLPVLVLAAVTVLLGTASEPLLVLATRAADQLL
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHC
DPQIYQRAVLGQLGSPALASIGGAP
CHHHHHHHHHHCCCCCHHHHCCCCC
>Mature Secondary Structure 
GLLLSLPLLLPLGSSLLLFVTRHNRRASHFVAITSATVLLVLVSGLTLQVARDGLLVVA
CHHHHHHHHHHCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHEEECCCEEEEE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CHHHHHHHHHHCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]