BacMap

Definition	Thermomicrobium roseum DSM 5159 plasmid unnamed, complete sequence.
Accession	NC_011961
Length	917,738

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The map label for this gene is fabG [C]

Identifier: 221635636

GI number: 221635636

Start: 218608

End: 219423

Strand: Reverse

Name: fabG [C]

Synonym: trd_A0230

Alternate gene names: 221635636

Gene position: 219423-218608 (Counterclockwise)

Preceding gene: 221635637

Following gene: 221635635

Centisome position: 23.91

GC content: 64.71

Gene sequence:

>816_bases
ATGGTCGAAGAACGATGGCTCGCGGGAATGGTCGCCCTCGTCACTGGAGCGGGACGCGGCGAGGACGGTGGCGGCGGAGC
TGGGATCGCCCGCCAACTCGCCCGGCGCGGTGCCCGCATCGCCGTGAACGACATTTCGGAAGAATTCGCCATGGCTACTG
TCGAGCAGATCCGCCAGCTTGGTCGCGAGGCGATCGCTGTCGTCGGCGATGTCTCCGACCCGAACGACGCCGATCGGATG
GTCGATGAAGTCGTCCGCCATTTCGGTCGGATCGACATTCTCGTCAACAACGCGGCGATCAGTGGTCTCATCCCAGCGGT
CGAGCGCCTCCCCAACGAGATTTGGCACCACCAGCTCGCCGTCGACCTCGATGGCCCCTTCTACATGAGTCGTGCTGCGC
TCCGTTACATGATTCCCCAGCGCTTCGGCCGCATCGTCAACATTTCCTCCTTCGCCGCTGTCCGTACCGGCTTCGTCGGC
GGCGTCACCTACACCACCGCGAAGACGGGGCTGCTCGGGCTCACCCGGCAGATGGCCTACGAGGTCGCCCAGTATGGCAT
CACAGTCAACGCGCTGATGCCGACCGGTCTCCTCAACCCGCGCGTGTTGCGTCTGCGCCCCGACTGGGTCCAAGGTGAAG
GCCCGCATCGCGCGATCCACCCCGAGGAGGAACTGGGCTGGATCGTCGCCTATCTGTGCCACCCCAACATGACGGCGATT
TCCGGTGCGGCGATCCCAGTCGATCGCGGTGTCTCCAACGGCATCGGCGACTTCGCTGAACTGAAGCGACGGAGCGGCAA
GGATACCGGAGACTGA

Upstream 100 bases:

>100_bases
TTCTCGCTGACGCGCTGGACTCTCCACTCCGCCATCTGAGCACGGACCACTCGAGTCCGATCGAGAACGCATCGGGTAAA
GAGAAAGGAGCACGTGGATC

Downstream 100 bases:

>100_bases
CGTGTCGTCCAGCAGTGGGCACGACGAACTCTCACGGGAAAGGAGCCGATCGTGATGATGATGTTCGGACAACTCTGCGA
TCTCAGTGGGCTCGTCGCAT

Product: 3-oxoacyl-[acyl-carrier-protein] reductase (3-ketoacyl-acyl carrier protein reductase)

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MVEERWLAGMVALVTGAGRGEDGGGGAGIARQLARRGARIAVNDISEEFAMATVEQIRQLGREAIAVVGDVSDPNDADRM
VDEVVRHFGRIDILVNNAAISGLIPAVERLPNEIWHHQLAVDLDGPFYMSRAALRYMIPQRFGRIVNISSFAAVRTGFVG
GVTYTTAKTGLLGLTRQMAYEVAQYGITVNALMPTGLLNPRVLRLRPDWVQGEGPHRAIHPEEELGWIVAYLCHPNMTAI
SGAAIPVDRGVSNGIGDFAELKRRSGKDTGD

Sequences:

>Translated_271_residues
MVEERWLAGMVALVTGAGRGEDGGGGAGIARQLARRGARIAVNDISEEFAMATVEQIRQLGREAIAVVGDVSDPNDADRM
VDEVVRHFGRIDILVNNAAISGLIPAVERLPNEIWHHQLAVDLDGPFYMSRAALRYMIPQRFGRIVNISSFAAVRTGFVG
GVTYTTAKTGLLGLTRQMAYEVAQYGITVNALMPTGLLNPRVLRLRPDWVQGEGPHRAIHPEEELGWIVAYLCHPNMTAI
SGAAIPVDRGVSNGIGDFAELKRRSGKDTGD
>Mature_271_residues
MVEERWLAGMVALVTGAGRGEDGGGGAGIARQLARRGARIAVNDISEEFAMATVEQIRQLGREAIAVVGDVSDPNDADRM
VDEVVRHFGRIDILVNNAAISGLIPAVERLPNEIWHHQLAVDLDGPFYMSRAALRYMIPQRFGRIVNISSFAAVRTGFVG
GVTYTTAKTGLLGLTRQMAYEVAQYGITVNALMPTGLLNPRVLRLRPDWVQGEGPHRAIHPEEELGWIVAYLCHPNMTAI
SGAAIPVDRGVSNGIGDFAELKRRSGKDTGD

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=252, Percent_Identity=31.3492063492063, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI5031737, Length=247, Percent_Identity=29.9595141700405, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI4504505, Length=235, Percent_Identity=33.6170212765957, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI19923817, Length=258, Percent_Identity=27.5193798449612, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI40254992, Length=243, Percent_Identity=31.2757201646091, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI33667109, Length=188, Percent_Identity=32.4468085106383, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI126723191, Length=186, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI15277342, Length=259, Percent_Identity=27.4131274131274, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI209862901, Length=257, Percent_Identity=27.6264591439689, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI19923983, Length=257, Percent_Identity=27.6264591439689, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4503301, Length=241, Percent_Identity=28.2157676348548, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI31542939, Length=235, Percent_Identity=29.3617021276596, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI59889578, Length=185, Percent_Identity=28.6486486486486, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI66933014, Length=232, Percent_Identity=24.5689655172414, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI40254986, Length=200, Percent_Identity=29.5, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI126723750, Length=257, Percent_Identity=27.2373540856031, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI10190704, Length=269, Percent_Identity=27.8810408921933, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI40807363, Length=194, Percent_Identity=29.3814432989691, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787335, Length=247, Percent_Identity=32.7935222672065, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789208, Length=206, Percent_Identity=35.9223300970874, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87082100, Length=255, Percent_Identity=31.7647058823529, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790717, Length=251, Percent_Identity=31.0756972111554, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788459, Length=246, Percent_Identity=31.3008130081301, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787905, Length=248, Percent_Identity=27.8225806451613, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082160, Length=191, Percent_Identity=30.8900523560209, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1789057, Length=223, Percent_Identity=32.7354260089686, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI2367175, Length=250, Percent_Identity=30.4, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789378, Length=185, Percent_Identity=30.2702702702703, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367365, Length=245, Percent_Identity=29.3877551020408, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788891, Length=237, Percent_Identity=27.4261603375527, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17568967, Length=219, Percent_Identity=35.6164383561644, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17561402, Length=259, Percent_Identity=25.8687258687259, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17555706, Length=254, Percent_Identity=28.740157480315, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17564282, Length=194, Percent_Identity=30.4123711340206, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI193203117, Length=177, Percent_Identity=31.0734463276836, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193203115, Length=175, Percent_Identity=31.4285714285714, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562910, Length=263, Percent_Identity=27.3764258555133, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=253, Percent_Identity=28.8537549407115, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560676, Length=255, Percent_Identity=25.4901960784314, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562904, Length=257, Percent_Identity=27.2373540856031, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71982365, Length=196, Percent_Identity=31.1224489795918, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560150, Length=265, Percent_Identity=27.9245283018868, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115534694, Length=251, Percent_Identity=29.0836653386454, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17562908, Length=288, Percent_Identity=24.6527777777778, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17560220, Length=266, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17567345, Length=211, Percent_Identity=27.4881516587678, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17536025, Length=272, Percent_Identity=28.3088235294118, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17544670, Length=218, Percent_Identity=27.5229357798165, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17551412, Length=198, Percent_Identity=28.2828282828283, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=194, Percent_Identity=28.3505154639175, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71997099, Length=206, Percent_Identity=30.5825242718447, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17536651, Length=244, Percent_Identity=25, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=131, Percent_Identity=29.0076335877863, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=267, Percent_Identity=27.7153558052434, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562990, Length=253, Percent_Identity=28.0632411067194, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562906, Length=196, Percent_Identity=25.5102040816327, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538480, Length=199, Percent_Identity=27.6381909547739, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI72000259, Length=194, Percent_Identity=25.7731958762887, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=270, Percent_Identity=31.1111111111111, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI18859837, Length=239, Percent_Identity=35.9832635983264, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24639444, Length=255, Percent_Identity=32.9411764705882, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21355319, Length=247, Percent_Identity=28.3400809716599, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI23397609, Length=257, Percent_Identity=33.0739299610895, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24644339, Length=256, Percent_Identity=27.734375, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21357673, Length=204, Percent_Identity=32.3529411764706, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355631, Length=182, Percent_Identity=31.3186813186813, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24649181, Length=197, Percent_Identity=33.502538071066, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24640492, Length=202, Percent_Identity=30.1980198019802, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21357041, Length=250, Percent_Identity=28, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 29323; Mature: 29323

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEERWLAGMVALVTGAGRGEDGGGGAGIARQLARRGARIAVNDISEEFAMATVEQIRQL
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHH
GREAIAVVGDVSDPNDADRMVDEVVRHFGRIDILVNNAAISGLIPAVERLPNEIWHHQLA
HHHHEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHEEE
VDLDGPFYMSRAALRYMIPQRFGRIVNISSFAAVRTGFVGGVTYTTAKTGLLGLTRQMAY
EECCCCHHHHHHHHHHHHHHHCCCEECHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
EVAQYGITVNALMPTGLLNPRVLRLRPDWVQGEGPHRAIHPEEELGWIVAYLCHPNMTAI
HHHHHCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHEEEEEECCCCHHC
SGAAIPVDRGVSNGIGDFAELKRRSGKDTGD
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVEERWLAGMVALVTGAGRGEDGGGGAGIARQLARRGARIAVNDISEEFAMATVEQIRQL
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHH
GREAIAVVGDVSDPNDADRMVDEVVRHFGRIDILVNNAAISGLIPAVERLPNEIWHHQLA
HHHHEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHEEE
VDLDGPFYMSRAALRYMIPQRFGRIVNISSFAAVRTGFVGGVTYTTAKTGLLGLTRQMAY
EECCCCHHHHHHHHHHHHHHHCCCEECHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
EVAQYGITVNALMPTGLLNPRVLRLRPDWVQGEGPHRAIHPEEELGWIVAYLCHPNMTAI
HHHHHCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHEEEEEECCCCHHC
SGAAIPVDRGVSNGIGDFAELKRRSGKDTGD
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]