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The map label for this gene is dgoA [H]

Identifier: 221633930

GI number: 221633930

Start: 1977016

End: 1978215

Strand: Reverse

Name: dgoA [H]

Synonym: trd_1958

Alternate gene names: 221633930

Gene position: 1978215-1977016 (Counterclockwise)

Preceding gene: 221633931

Following gene: 221633928

Centisome position: 98.76

GC content: 54.67

Gene sequence:

>1200_bases
ATGCGTATCGTAGCGCTGGAAACGCTTCGCCTTAATGAATTTCCAAATCTTCTCTGGGTCCGTCTTCATACGGATAAAGG
ATACGTTGGGATTGGAGAAACGTTTTTCGGTGCACAAGCCGTTGAATCCTATCTCCATGAGTCGGTCGCGCCCTATCTGC
TTGGTCAGGATCCACGCGACATTCAACGACACGCACGTGCGCTCAGGCAATATGTTGGATTCTGCGGAACTGGTGTCGAG
CAGCGCGGTAACTCCGCTATCGACTTAGCACTCTGGGATCTGATTGGTCGCGCTACTGGACAACCGCTATACCAGGCACT
CGGCGGTCGCGTCCGAACGGAGATTCCTGTTTACAACACCTGTGCCGGCTATCGTTACATTCGAGCGCAGCCTCGCCAGG
CAGTCGAGAACTGGGGACTCCCTGAGAACCCTTCTGAGGGCCCCTATGAAGATCTCGAAGCTTTCCTGACACGCCCGGAT
GCCCTGGCACGCGATCTTCTTGCGATGGGGATTCGGGCCATGAAAATATGGCCTTTTGATCCTGAAGCCGAAAGGACGAA
CGGGCAGTTTTTGAGCCTTTCTGATCTTGAGCGCTGCCTGGAACCGTTCCAACGGATACGCGCCACTGTTGGTCAAGAGA
TGGAGCTTCTTGTCGAACTCCACGGGCTCTGGAATCTTCCCATGGCCGTGCGCATTGCTCGGGCACTCGAACCGCTGAAA
CCTCTCTGGATCGAGGATCCCATTCGGAGTGATGATCTTGATGCCCTTGCCACGCTGGCGCGCGTAACACCCATTCCGCT
TGCGTTGAGCGAGACACTCGGACCCCGCTCAGCGTTTCGACAACTCCTTGAGCAGCGCATCCCTGGTTTCATACTGTTCG
ACCTCGGATGGTGCGGCGGGCTGACGGAAGCTCGAGCTATTGCTGCCATGGCTGAAGCTTATCAGCTACCTGTTGCCCCT
CATGACTGCACTGGGCCTATTGTTTTCACTGCTTCCGTACACTTCTCCTTGTCGATACCGAATTCTTTCATTCAGGAATT
CGTGCGAGCCTTCTACTTCGGTTGGTATCAAGAGATTGTCACCAACCTACCACCATTTGCTAACGGCCTCATTTCTGCCC
CTGAAGAAGCGGGACTAGGTACTGACCTGCGTCCGGAACTTTGGGAACGCTCGGACGTCCATATTCGGATCAGTCATTGA

Upstream 100 bases:

>100_bases
ATGGTGACGATCGCTCATCGGATAGCCTCATAGAGGCAATGATCCATGTTATAATATGCTGGACACCAATCATGCTTGGG
AAAGCGAAAGGAAGCTCGAT

Downstream 100 bases:

>100_bases
CGAGACGCCTCATATGGGGAGAGCTTGGGCAACAAAAAGACGCTAAAGCCTGCCCGCTGATACTCCGAACGATTTTCCTG
CCGAATGAGTGGAGGGAGAG

Product: putative galactonate dehydratase protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MRIVALETLRLNEFPNLLWVRLHTDKGYVGIGETFFGAQAVESYLHESVAPYLLGQDPRDIQRHARALRQYVGFCGTGVE
QRGNSAIDLALWDLIGRATGQPLYQALGGRVRTEIPVYNTCAGYRYIRAQPRQAVENWGLPENPSEGPYEDLEAFLTRPD
ALARDLLAMGIRAMKIWPFDPEAERTNGQFLSLSDLERCLEPFQRIRATVGQEMELLVELHGLWNLPMAVRIARALEPLK
PLWIEDPIRSDDLDALATLARVTPIPLALSETLGPRSAFRQLLEQRIPGFILFDLGWCGGLTEARAIAAMAEAYQLPVAP
HDCTGPIVFTASVHFSLSIPNSFIQEFVRAFYFGWYQEIVTNLPPFANGLISAPEEAGLGTDLRPELWERSDVHIRISH

Sequences:

>Translated_399_residues
MRIVALETLRLNEFPNLLWVRLHTDKGYVGIGETFFGAQAVESYLHESVAPYLLGQDPRDIQRHARALRQYVGFCGTGVE
QRGNSAIDLALWDLIGRATGQPLYQALGGRVRTEIPVYNTCAGYRYIRAQPRQAVENWGLPENPSEGPYEDLEAFLTRPD
ALARDLLAMGIRAMKIWPFDPEAERTNGQFLSLSDLERCLEPFQRIRATVGQEMELLVELHGLWNLPMAVRIARALEPLK
PLWIEDPIRSDDLDALATLARVTPIPLALSETLGPRSAFRQLLEQRIPGFILFDLGWCGGLTEARAIAAMAEAYQLPVAP
HDCTGPIVFTASVHFSLSIPNSFIQEFVRAFYFGWYQEIVTNLPPFANGLISAPEEAGLGTDLRPELWERSDVHIRISH
>Mature_399_residues
MRIVALETLRLNEFPNLLWVRLHTDKGYVGIGETFFGAQAVESYLHESVAPYLLGQDPRDIQRHARALRQYVGFCGTGVE
QRGNSAIDLALWDLIGRATGQPLYQALGGRVRTEIPVYNTCAGYRYIRAQPRQAVENWGLPENPSEGPYEDLEAFLTRPD
ALARDLLAMGIRAMKIWPFDPEAERTNGQFLSLSDLERCLEPFQRIRATVGQEMELLVELHGLWNLPMAVRIARALEPLK
PLWIEDPIRSDDLDALATLARVTPIPLALSETLGPRSAFRQLLEQRIPGFILFDLGWCGGLTEARAIAAMAEAYQLPVAP
HDCTGPIVFTASVHFSLSIPNSFIQEFVRAFYFGWYQEIVTNLPPFANGLISAPEEAGLGTDLRPELWERSDVHIRISH

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=412, Percent_Identity=23.3009708737864, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI42544117, Length=425, Percent_Identity=21.8823529411765, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI186972148, Length=341, Percent_Identity=23.4604105571848, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI48994953, Length=395, Percent_Identity=29.1139240506329, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1787864, Length=400, Percent_Identity=25.25, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI226510960, Length=382, Percent_Identity=26.1780104712042, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 44802; Mature: 44802

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVALETLRLNEFPNLLWVRLHTDKGYVGIGETFFGAQAVESYLHESVAPYLLGQDPRD
CEEEEEEHHHHCCCCCEEEEEEECCCCCEECCHHHHHHHHHHHHHHHHHCCHHCCCCHHH
IQRHARALRQYVGFCGTGVEQRGNSAIDLALWDLIGRATGQPLYQALGGRVRTEIPVYNT
HHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHCCCCEEECCCCCCC
CAGYRYIRAQPRQAVENWGLPENPSEGPYEDLEAFLTRPDALARDLLAMGIRAMKIWPFD
CCCCHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCHHHEEECCCC
PEAERTNGQFLSLSDLERCLEPFQRIRATVGQEMELLVELHGLWNLPMAVRIARALEPLK
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
PLWIEDPIRSDDLDALATLARVTPIPLALSETLGPRSAFRQLLEQRIPGFILFDLGWCGG
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCC
LTEARAIAAMAEAYQLPVAPHDCTGPIVFTASVHFSLSIPNSFIQEFVRAFYFGWYQEIV
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHH
TNLPPFANGLISAPEEAGLGTDLRPELWERSDVHIRISH
HCCCCHHHHCCCCCHHCCCCCCCCHHHHCCCCCEEEECC
>Mature Secondary Structure
MRIVALETLRLNEFPNLLWVRLHTDKGYVGIGETFFGAQAVESYLHESVAPYLLGQDPRD
CEEEEEEHHHHCCCCCEEEEEEECCCCCEECCHHHHHHHHHHHHHHHHHCCHHCCCCHHH
IQRHARALRQYVGFCGTGVEQRGNSAIDLALWDLIGRATGQPLYQALGGRVRTEIPVYNT
HHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHCCCCEEECCCCCCC
CAGYRYIRAQPRQAVENWGLPENPSEGPYEDLEAFLTRPDALARDLLAMGIRAMKIWPFD
CCCCHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCHHHEEECCCC
PEAERTNGQFLSLSDLERCLEPFQRIRATVGQEMELLVELHGLWNLPMAVRIARALEPLK
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
PLWIEDPIRSDDLDALATLARVTPIPLALSETLGPRSAFRQLLEQRIPGFILFDLGWCGG
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCC
LTEARAIAAMAEAYQLPVAPHDCTGPIVFTASVHFSLSIPNSFIQEFVRAFYFGWYQEIV
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHH
TNLPPFANGLISAPEEAGLGTDLRPELWERSDVHIRISH
HCCCCHHHHCCCCCHHCCCCCCCCHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA