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The map label for this gene is dgoD [H]

Identifier: 221633918

GI number: 221633918

Start: 1963834

End: 1965027

Strand: Reverse

Name: dgoD [H]

Synonym: trd_1945

Alternate gene names: 221633918

Gene position: 1965027-1963834 (Counterclockwise)

Preceding gene: 221633919

Following gene: 221633917

Centisome position: 98.1

GC content: 61.73

Gene sequence:

>1194_bases
GTGCGGATTGTTGATTTCCGCTGTGCAGTCATCGCGGGAAGCCCGGTCGTCCGGATCGTGACAGACGAGGGGATCGACGG
CTACGGCCAGGTCGAGTCGACGAAGCCGTATGTAAAACCCCAGCTTCTGCTCTTTCGCGACCACCTTCTCGGCGAGGATC
CCACCGATGTCGAGCGAGTCATGCGGAAGATCAGGCGCCTGGGCTCATTCAAGCCGTGGGGAAGCGCCGTCAGCGCGATC
GAGATGGCTCTCTGGGACATTACCGGCAAAGCCTACGGGATCCCCGTCTACAAGCTCCTCGGTGGCAAGGTCCGCGATCG
CGTCCGTGTCTACAACGGCGGGGTACGCCAGCCACTGCGCGGATACGCTCCCGAGGACTTCGTCGAGCATGTCGCGTTCA
TGAAGCGTGCCCGCGAGTGCTTCAGTATCGTCAAAGTGGCACTGGGCTACCACGGGCCGATGCTGAACGCGATCCCCGGC
GCCCGATATGGCGAGCCGGTGAGCGGTGCCGCTCCCTACCTCAACCGAGGGCCTCTCACCGAACGCGGCCTGCGTCATCT
CGTAGCCTGCGTCGAAGCGATGAAGGCGGAACTCGGCGACGAGATCGGGCTCGCACTCGACTGCGGCCCCGGTTGGACCG
TTCCGGACGCGATCCGCTTTGCGCGAGCTATCGAGCACTTGAACGTCCTTTGGCTCGAAGACTTGATCACCGGTGACTAT
ACGCCGTACGTCCTTGCTGACCTCTACCGCGAGGTGACAACCCAGACGACCGTGCCGATCCATACCGGTGAGCAGATCTA
TCTCCGGCAGAACTTCGTCGAGCTCATCGAGCGGCATGCGGTCCGCGTGATCGGCCCGGACCCAGCGGATGTCGGCGGCC
TGGCGGAACTCAAGTGGATCGCCGAATATGCCGACCTTCACGGTCTCCTCATCGCACCACACGGGATCTTCAATGGACTT
ATTGGTCTTGCTGCGCTGGTTCACGTCTGCGCGACCTTGCCGGATAACTTCATCGCCTTCGAGTATCCGGTCGCCAAGCC
GGACTGGTGGTATAACATCGTTGTCGGCTTGCCTGATCCGATCGTCGTCGATGGCTGCATCACAGTCTGGGATCGGCCAG
GTCTCGGTGTGCACTTCGATACCGAGAAGGCGAGCCGGTATCTCGCTGAGGAAGACCAAGGCTTCTTCGATTGA

Upstream 100 bases:

>100_bases
TCTGATCGCCAAAGCTCATCTGTTCCCAGCGCTGTGTTCTAGCCCAAAACTCTTCGTGTAGACTCTTACGAGGAGCGCGT
CGAGATCACGAGGTGGAACT

Downstream 100 bases:

>100_bases
CTCGGCTGTATGGCTGTAAGGGGAGAGTGATGGTCGTCAACTCCGCTATTCCCGACATCGAGCGACCGCCGCGCGAGCTA
CTCGACGCGCTCCAGGCCGA

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MRIVDFRCAVIAGSPVVRIVTDEGIDGYGQVESTKPYVKPQLLLFRDHLLGEDPTDVERVMRKIRRLGSFKPWGSAVSAI
EMALWDITGKAYGIPVYKLLGGKVRDRVRVYNGGVRQPLRGYAPEDFVEHVAFMKRARECFSIVKVALGYHGPMLNAIPG
ARYGEPVSGAAPYLNRGPLTERGLRHLVACVEAMKAELGDEIGLALDCGPGWTVPDAIRFARAIEHLNVLWLEDLITGDY
TPYVLADLYREVTTQTTVPIHTGEQIYLRQNFVELIERHAVRVIGPDPADVGGLAELKWIAEYADLHGLLIAPHGIFNGL
IGLAALVHVCATLPDNFIAFEYPVAKPDWWYNIVVGLPDPIVVDGCITVWDRPGLGVHFDTEKASRYLAEEDQGFFD

Sequences:

>Translated_397_residues
MRIVDFRCAVIAGSPVVRIVTDEGIDGYGQVESTKPYVKPQLLLFRDHLLGEDPTDVERVMRKIRRLGSFKPWGSAVSAI
EMALWDITGKAYGIPVYKLLGGKVRDRVRVYNGGVRQPLRGYAPEDFVEHVAFMKRARECFSIVKVALGYHGPMLNAIPG
ARYGEPVSGAAPYLNRGPLTERGLRHLVACVEAMKAELGDEIGLALDCGPGWTVPDAIRFARAIEHLNVLWLEDLITGDY
TPYVLADLYREVTTQTTVPIHTGEQIYLRQNFVELIERHAVRVIGPDPADVGGLAELKWIAEYADLHGLLIAPHGIFNGL
IGLAALVHVCATLPDNFIAFEYPVAKPDWWYNIVVGLPDPIVVDGCITVWDRPGLGVHFDTEKASRYLAEEDQGFFD
>Mature_397_residues
MRIVDFRCAVIAGSPVVRIVTDEGIDGYGQVESTKPYVKPQLLLFRDHLLGEDPTDVERVMRKIRRLGSFKPWGSAVSAI
EMALWDITGKAYGIPVYKLLGGKVRDRVRVYNGGVRQPLRGYAPEDFVEHVAFMKRARECFSIVKVALGYHGPMLNAIPG
ARYGEPVSGAAPYLNRGPLTERGLRHLVACVEAMKAELGDEIGLALDCGPGWTVPDAIRFARAIEHLNVLWLEDLITGDY
TPYVLADLYREVTTQTTVPIHTGEQIYLRQNFVELIERHAVRVIGPDPADVGGLAELKWIAEYADLHGLLIAPHGIFNGL
IGLAALVHVCATLPDNFIAFEYPVAKPDWWYNIVVGLPDPIVVDGCITVWDRPGLGVHFDTEKASRYLAEEDQGFFD

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=383, Percent_Identity=25.3263707571802, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787864, Length=389, Percent_Identity=26.4781491002571, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 44043; Mature: 44043

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVDFRCAVIAGSPVVRIVTDEGIDGYGQVESTKPYVKPQLLLFRDHLLGEDPTDVERV
CEEEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
MRKIRRLGSFKPWGSAVSAIEMALWDITGKAYGIPVYKLLGGKVRDRVRVYNGGVRQPLR
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCC
GYAPEDFVEHVAFMKRARECFSIVKVALGYHGPMLNAIPGARYGEPVSGAAPYLNRGPLT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCH
ERGLRHLVACVEAMKAELGDEIGLALDCGPGWTVPDAIRFARAIEHLNVLWLEDLITGDY
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TPYVLADLYREVTTQTTVPIHTGEQIYLRQNFVELIERHAVRVIGPDPADVGGLAELKWI
CHHHHHHHHHHHHCCCEEEEECCCEEEHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHH
AEYADLHGLLIAPHGIFNGLIGLAALVHVCATLPDNFIAFEYPVAKPDWWYNIVVGLPDP
HHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCC
IVVDGCITVWDRPGLGVHFDTEKASRYLAEEDQGFFD
EEECCEEEEECCCCCCEEECHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRIVDFRCAVIAGSPVVRIVTDEGIDGYGQVESTKPYVKPQLLLFRDHLLGEDPTDVERV
CEEEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
MRKIRRLGSFKPWGSAVSAIEMALWDITGKAYGIPVYKLLGGKVRDRVRVYNGGVRQPLR
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCC
GYAPEDFVEHVAFMKRARECFSIVKVALGYHGPMLNAIPGARYGEPVSGAAPYLNRGPLT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCH
ERGLRHLVACVEAMKAELGDEIGLALDCGPGWTVPDAIRFARAIEHLNVLWLEDLITGDY
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TPYVLADLYREVTTQTTVPIHTGEQIYLRQNFVELIERHAVRVIGPDPADVGGLAELKWI
CHHHHHHHHHHHHCCCEEEEECCCEEEHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHH
AEYADLHGLLIAPHGIFNGLIGLAALVHVCATLPDNFIAFEYPVAKPDWWYNIVVGLPDP
HHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCC
IVVDGCITVWDRPGLGVHFDTEKASRYLAEEDQGFFD
EEECCEEEEECCCCCCEEECHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA