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The map label for this gene is hyb0 [C]

Identifier: 221633851

GI number: 221633851

Start: 1887363

End: 1888403

Strand: Reverse

Name: hyb0 [C]

Synonym: trd_1878

Alternate gene names: 221633851

Gene position: 1888403-1887363 (Counterclockwise)

Preceding gene: 221633854

Following gene: 221633850

Centisome position: 94.28

GC content: 65.13

Gene sequence:

>1041_bases
ATGACTGCCCGCATGACGGAAAGTGTGCCGGAGGTTCACATTCTCTGGATCAACGCCGGGCTGAGTTGCGACGGTGACTC
CGTGGCCCTCACAGCGGCGACCCAGCCGACGATCGAGGAGATCGCGCTTGGTGCCCTGCCCGGGTTGCCACGCGTGCATC
TCCACTGGCCTCTGATCGACTACGCAACGGGCGAGGACTTCCTCTCCTGGTTCTTCCGGGCCGAGCGCGGCGAGCTCGAG
CCCTTCATCCTCGTCGTGGAAGGCTCCATTCCCAACGAGGCAGTGAAGCGCGAGGGATACTGGTGCGGCTTCGGCAATGA
CCCCGCCACCGGACAACCGATCACGACGAGCGAGTGGCTCGACCGGCTGGCACCGAAGGCCTGGGCGGTCGTGGCCGCTG
GGACCTGCGCGACCTATGGCGGCATCCACGCCATGGCCGGCAACCCGACCGGAGCGATGGGCGTCCCCGACTATCTCGGC
TGGAACTGGCGCTCCAAGGCTGGCATCCCGGTCGTCTGTGTGCCGGGATGCCCGATCCACCCTGACAACATGGCCGAGAC
GCTCCTCTATCTCCTCTACCAAGCAGCTGGACGCGCTCCCATGATCCCGCTCGACGAGGCACTCCGTCCCAAGTGGCTCT
TCGGCGCGACGGTCCACCAGGGCTGTGACCGAGCGGGTTACTACGAGGAAGGAGACTTCGCCACCGAGTATGGCTCGCCG
AAGTGCCTGGTGAAGCTCGGCTGTTGGGGTCCGGTGGTGAAGTGCAACGTGCCGAAGCGTGGCTGGATCAACGGGATCGG
GGGTTGTCCGAACGTGGGCGGTATCTGCATCGCCTGCACCATGCCGGGGTTCCCCGACAAGTTCATGCCCTTCATGGATG
CCCCACCGGGTAGCGTCGTGTCCGGGACGCTGAGCCAAGTCTACGGTACCGTGATCCGCGGTCTCCGGCAGATCACCTTA
CAGACCGTCGATAAGGAGCCGAAGTGGCGCCGCAAGGGGAGCGAGCTGGCCACCGGGTACGAGAAGACTCGCTATGACTA
G

Upstream 100 bases:

>100_bases
ACGAGGCTCACCGCTGTGGCATCCCGATTGACAAGCAGGAATGTGACTGATATCATATCAGGCAACCAGACCACCATCGG
TCCAATAAGGGAGGTGCCTC

Downstream 100 bases:

>100_bases
GCAGCCGGCACCTCAGGCTTGACAGGGAAGGAGCGGACCGATGGCAGAGGCAGGCACGACCCGAGAACTCGTCGAGATGT
CCTGGGACCCGATCACCCGC

Product: nickel-dependent hydrogenase small subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MTARMTESVPEVHILWINAGLSCDGDSVALTAATQPTIEEIALGALPGLPRVHLHWPLIDYATGEDFLSWFFRAERGELE
PFILVVEGSIPNEAVKREGYWCGFGNDPATGQPITTSEWLDRLAPKAWAVVAAGTCATYGGIHAMAGNPTGAMGVPDYLG
WNWRSKAGIPVVCVPGCPIHPDNMAETLLYLLYQAAGRAPMIPLDEALRPKWLFGATVHQGCDRAGYYEEGDFATEYGSP
KCLVKLGCWGPVVKCNVPKRGWINGIGGCPNVGGICIACTMPGFPDKFMPFMDAPPGSVVSGTLSQVYGTVIRGLRQITL
QTVDKEPKWRRKGSELATGYEKTRYD

Sequences:

>Translated_346_residues
MTARMTESVPEVHILWINAGLSCDGDSVALTAATQPTIEEIALGALPGLPRVHLHWPLIDYATGEDFLSWFFRAERGELE
PFILVVEGSIPNEAVKREGYWCGFGNDPATGQPITTSEWLDRLAPKAWAVVAAGTCATYGGIHAMAGNPTGAMGVPDYLG
WNWRSKAGIPVVCVPGCPIHPDNMAETLLYLLYQAAGRAPMIPLDEALRPKWLFGATVHQGCDRAGYYEEGDFATEYGSP
KCLVKLGCWGPVVKCNVPKRGWINGIGGCPNVGGICIACTMPGFPDKFMPFMDAPPGSVVSGTLSQVYGTVIRGLRQITL
QTVDKEPKWRRKGSELATGYEKTRYD
>Mature_345_residues
TARMTESVPEVHILWINAGLSCDGDSVALTAATQPTIEEIALGALPGLPRVHLHWPLIDYATGEDFLSWFFRAERGELEP
FILVVEGSIPNEAVKREGYWCGFGNDPATGQPITTSEWLDRLAPKAWAVVAAGTCATYGGIHAMAGNPTGAMGVPDYLGW
NWRSKAGIPVVCVPGCPIHPDNMAETLLYLLYQAAGRAPMIPLDEALRPKWLFGATVHQGCDRAGYYEEGDFATEYGSPK
CLVKLGCWGPVVKCNVPKRGWINGIGGCPNVGGICIACTMPGFPDKFMPFMDAPPGSVVSGTLSQVYGTVIRGLRQITLQ
TVDKEPKWRRKGSELATGYEKTRYD

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=257, Percent_Identity=31.1284046692607, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787206, Length=288, Percent_Identity=26.7361111111111, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 37492; Mature: 37361

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARMTESVPEVHILWINAGLSCDGDSVALTAATQPTIEEIALGALPGLPRVHLHWPLID
CCCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCEEEEECCEEE
YATGEDFLSWFFRAERGELEPFILVVEGSIPNEAVKREGYWCGFGNDPATGQPITTSEWL
CCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCEEEECCCCCCCCCCCCHHHHH
DRLAPKAWAVVAAGTCATYGGIHAMAGNPTGAMGVPDYLGWNWRSKAGIPVVCVPGCPIH
HHHCCCCEEEEECCCHHHHCCEEEECCCCCCCCCCCHHHCCCCCCCCCCCEEECCCCCCC
PDNMAETLLYLLYQAAGRAPMIPLDEALRPKWLFGATVHQGCDRAGYYEEGDFATEYGSP
CCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEECCHHHHCCCCCCCCCCCCEECCCCCC
KCLVKLGCWGPVVKCNVPKRGWINGIGGCPNVGGICIACTMPGFPDKFMPFMDAPPGSVV
EEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCCCCCHHH
SGTLSQVYGTVIRGLRQITLQTVDKEPKWRRKGSELATGYEKTRYD
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHCCCHHHCCC
>Mature Secondary Structure 
TARMTESVPEVHILWINAGLSCDGDSVALTAATQPTIEEIALGALPGLPRVHLHWPLID
CCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCEEEEECCEEE
YATGEDFLSWFFRAERGELEPFILVVEGSIPNEAVKREGYWCGFGNDPATGQPITTSEWL
CCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCEEEECCCCCCCCCCCCHHHHH
DRLAPKAWAVVAAGTCATYGGIHAMAGNPTGAMGVPDYLGWNWRSKAGIPVVCVPGCPIH
HHHCCCCEEEEECCCHHHHCCEEEECCCCCCCCCCCHHHCCCCCCCCCCCEEECCCCCCC
PDNMAETLLYLLYQAAGRAPMIPLDEALRPKWLFGATVHQGCDRAGYYEEGDFATEYGSP
CCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEECCHHHHCCCCCCCCCCCCEECCCCCC
KCLVKLGCWGPVVKCNVPKRGWINGIGGCPNVGGICIACTMPGFPDKFMPFMDAPPGSVV
EEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCCCCCHHH
SGTLSQVYGTVIRGLRQITLQTVDKEPKWRRKGSELATGYEKTRYD
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]