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The map label for this gene is ispH [H]

Identifier: 221633636

GI number: 221633636

Start: 1659320

End: 1660282

Strand: Reverse

Name: ispH [H]

Synonym: trd_1662

Alternate gene names: 221633636

Gene position: 1660282-1659320 (Counterclockwise)

Preceding gene: 221633637

Following gene: 221633635

Centisome position: 82.89

GC content: 66.56

Gene sequence:

>963_bases
ATGACCGTAGCGACGGGTGAGAAGCGAATCGTCCTCGCGGAAGTCATGGGTTACTGTTGGGGTGTGCGCCGAGCGTTGGA
GATCATCCAGCAGGCTGCCCAAGCTGGCCCGATCGCGACGGTCGGTGACGTGATCCACAATCCGCAAGTCGTCGAACGGC
TGAAGGTCCAGGGGATCGAGCCGGTCAGCTCGGTGAGCGAAGCGCGCGAGCGTGGTTTCCGGCGCGTGGCGATCACGGCG
CACGGGGCGGGACCGCAGCGTGCGGCGGAAGCCCGCGACGCTGGACTGGAGCTGATCGACACGACCTGCCCACTGGTGAC
CAAAGTACAGCGCCTGGCCGAGAAGCTGGTGCGTCAGGGCTACTTTCTCGTCGTCTACGGCGATGCTCACCACCCTGAGG
TGCGCGGCGTGCTCGGTTGGGCCGGAACCTCACGGGCGGTTGCGGCCAAGCATTTGGAGGATTTGCCGTGGAAAGGGCCG
CGCGGCGTGCCGGGTGTCGTGGCGCCGCCGCGTAAGGTCGCGGTGGTGAGTCAAACGACCAAGCAGACAGACGAGTTCCT
GGCCTTCGCGCTCGGCCTGGCCAATTGGGTAGCCCGGCATGGGGGCGAGATCCGGATCGTCAATACCATCTGCCAACCGA
CGTGGGAGCGGCAGGAAGCGTTGCGCGCGCTGGCCCGCGAAGTCGATGTCCTGCTCGCAGTGGGTGGAAAGAAGAGTTCC
AACACGGCGCGCCTCGCCGAAGTCGGCCGCGCCTGTGGTGTTCCCAGCTACCACATCGAGCGACCGGAGGAAATCGACCG
TGCGTGGCTCGAGGGGAAGCGGGTGGTCGGGGTGACGGCTGGTGCCTCGACGCCTGACGAGGTCGTGCTCGCGGTGGTCG
ATCACCTGGCGGCGCTCGGGTACCAGCGCCCCGAACGCTTGTGGCGCTACGAGGCACCGGATCTCGACGATTTCGCCGAA
TAG

Upstream 100 bases:

>100_bases
TATCGGGAATTGGAGGAATGGGCGAGCAGCGGGAGAGCGCTTTTCCCCGGGGGTATACTCGGTGAGAAACGGGGAGCGGC
GCGTTCGCGAGGAGGCGACG

Downstream 100 bases:

>100_bases
CGAAGGGCAGTGCGAGAGCGCTATGGACTGCCGGCGTCGAGTTCCAGGAGTATTCGCGCTCGCAGGTGCTCTCCTGCTCT
ACGTGCTGCTGGCGCTCTGG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTVATGEKRIVLAEVMGYCWGVRRALEIIQQAAQAGPIATVGDVIHNPQVVERLKVQGIEPVSSVSEARERGFRRVAITA
HGAGPQRAAEARDAGLELIDTTCPLVTKVQRLAEKLVRQGYFLVVYGDAHHPEVRGVLGWAGTSRAVAAKHLEDLPWKGP
RGVPGVVAPPRKVAVVSQTTKQTDEFLAFALGLANWVARHGGEIRIVNTICQPTWERQEALRALAREVDVLLAVGGKKSS
NTARLAEVGRACGVPSYHIERPEEIDRAWLEGKRVVGVTAGASTPDEVVLAVVDHLAALGYQRPERLWRYEAPDLDDFAE

Sequences:

>Translated_320_residues
MTVATGEKRIVLAEVMGYCWGVRRALEIIQQAAQAGPIATVGDVIHNPQVVERLKVQGIEPVSSVSEARERGFRRVAITA
HGAGPQRAAEARDAGLELIDTTCPLVTKVQRLAEKLVRQGYFLVVYGDAHHPEVRGVLGWAGTSRAVAAKHLEDLPWKGP
RGVPGVVAPPRKVAVVSQTTKQTDEFLAFALGLANWVARHGGEIRIVNTICQPTWERQEALRALAREVDVLLAVGGKKSS
NTARLAEVGRACGVPSYHIERPEEIDRAWLEGKRVVGVTAGASTPDEVVLAVVDHLAALGYQRPERLWRYEAPDLDDFAE
>Mature_319_residues
TVATGEKRIVLAEVMGYCWGVRRALEIIQQAAQAGPIATVGDVIHNPQVVERLKVQGIEPVSSVSEARERGFRRVAITAH
GAGPQRAAEARDAGLELIDTTCPLVTKVQRLAEKLVRQGYFLVVYGDAHHPEVRGVLGWAGTSRAVAAKHLEDLPWKGPR
GVPGVVAPPRKVAVVSQTTKQTDEFLAFALGLANWVARHGGEIRIVNTICQPTWERQEALRALAREVDVLLAVGGKKSSN
TARLAEVGRACGVPSYHIERPEEIDRAWLEGKRVVGVTAGASTPDEVVLAVVDHLAALGYQRPERLWRYEAPDLDDFAE

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=295, Percent_Identity=34.2372881355932, Blast_Score=144, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 34794; Mature: 34663

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVATGEKRIVLAEVMGYCWGVRRALEIIQQAAQAGPIATVGDVIHNPQVVERLKVQGIE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCCC
PVSSVSEARERGFRRVAITAHGAGPQRAAEARDAGLELIDTTCPLVTKVQRLAEKLVRQG
HHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCC
YFLVVYGDAHHPEVRGVLGWAGTSRAVAAKHLEDLPWKGPRGVPGVVAPPRKVAVVSQTT
CEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHH
KQTDEFLAFALGLANWVARHGGEIRIVNTICQPTWERQEALRALAREVDVLLAVGGKKSS
HHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHEEECCCCCC
NTARLAEVGRACGVPSYHIERPEEIDRAWLEGKRVVGVTAGASTPDEVVLAVVDHLAALG
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHC
YQRPERLWRYEAPDLDDFAE
CCCHHHHHCCCCCCCHHHCC
>Mature Secondary Structure 
TVATGEKRIVLAEVMGYCWGVRRALEIIQQAAQAGPIATVGDVIHNPQVVERLKVQGIE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCCC
PVSSVSEARERGFRRVAITAHGAGPQRAAEARDAGLELIDTTCPLVTKVQRLAEKLVRQG
HHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCC
YFLVVYGDAHHPEVRGVLGWAGTSRAVAAKHLEDLPWKGPRGVPGVVAPPRKVAVVSQTT
CEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHH
KQTDEFLAFALGLANWVARHGGEIRIVNTICQPTWERQEALRALAREVDVLLAVGGKKSS
HHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHEEECCCCCC
NTARLAEVGRACGVPSYHIERPEEIDRAWLEGKRVVGVTAGASTPDEVVLAVVDHLAALG
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHC
YQRPERLWRYEAPDLDDFAE
CCCHHHHHCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA