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The map label for this gene is thiF [H]

Identifier: 221633544

GI number: 221633544

Start: 1552795

End: 1553826

Strand: Reverse

Name: thiF [H]

Synonym: trd_1568

Alternate gene names: 221633544

Gene position: 1553826-1552795 (Counterclockwise)

Preceding gene: 221633546

Following gene: 221633543

Centisome position: 77.57

GC content: 66.38

Gene sequence:

>1032_bases
ATGACCAGCGACGAGTCAGCAGAGCGCCATCACCGTCAGATCCTTCTCCCGGTCATCGGACGAGCTGGCCAATCCCGCAT
CCGAGCGGGACGCGTCTGCGTCCTCGGGCTGGGGGCACTCGGGAGTCACACGACCATGCTGCTCGCCCGAGCGGGTGTCG
GCTTCCTCCGGCTGGTCGATCGCGACGTGGTCGAGTGGACGAACCTGCAGCGTCAGGCGCTCTACGAGGAAGAGGACGTG
CGGGCCGCGCTCCCGAAGGCGGTCGCGGCCGCGCGAGCCGTCCAGCGGATCGATCAGGCGATCGCGGTGGAGCCGGTTGT
CCTCGATGTCGATGCGGCATCCATCACTGGGCTAATTGCCGACGTCGATGTCGTGGTCGATGGGAGCGACAACTTCGAGC
TACGCTACCTCTTGAACGATGCAGCGCTGGAATTGGGGAGACCCTGGGTCTACGGTGGCGTGATCGGGACACATGGCTCC
ACGATGACGATCGTTCCCGACGAGACAGCCTGCCTTCGCTGCCTGTTCCCCGATCCGCCAGCACCAGGCAGCGTTCCCAC
CTGTGACACGGTCGGTGTGCTCGGCCCCGCTGCGGCGCTGGTCGGTGCCCTGCAAGCTAGCGAGACGCTCAAGCTCCTCG
TGGGCGACCGGGCCGCTCGCAACCCTGGCCTGCTCTCCATCGATCTCTGGAAGGTGGAGATCGCGCGCATCGCCGTGGAG
CGCCGGTCGGATTGCCCGGCTTGTGGCCGAGGCGAGCGTGCCTTCCTCCGCCAGCAGGTGGCGAATCGCATCGTCTCCCT
GTGCGGACGCGATGCCGTCCAGGTCGTCGTGCGACCCGCCGTCTCGCTCGATCTGGCAGCGCTCGCTGAGCGCCTGTCGG
CACTCGGGTCGCTCCAGCATAACCCGTACCTGATACGATTCATGGTGGAGGGTTATGAGTTGACGGTCTTCCCGGACGGA
CGAGCCATCGTGCGTGGGACGACCGATCCTGCGATTGCCCGCGTGCTCTACGCGCGGTACATCGGGATGTGA

Upstream 100 bases:

>100_bases
CGGGCCGCTGCTCCTCGGACGCACGGGAGCAGTGGCCAGCCCGCTTGGGACGAGCCGGATGGCAACTGTACCATATATAT
CGAGAGGGCGGGAACACGCG

Downstream 100 bases:

>100_bases
CGGGGATGATGAGTTATTTGGACAACGCCACGACCAGCTGGCCCAAACCGGATGTCGTGCGCGAAACGCTCGCTGGCTTC
CTGGCGACGGCGAGCGGTGC

Product: putative molybdopterin biosynthesis MoeB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MTSDESAERHHRQILLPVIGRAGQSRIRAGRVCVLGLGALGSHTTMLLARAGVGFLRLVDRDVVEWTNLQRQALYEEEDV
RAALPKAVAAARAVQRIDQAIAVEPVVLDVDAASITGLIADVDVVVDGSDNFELRYLLNDAALELGRPWVYGGVIGTHGS
TMTIVPDETACLRCLFPDPPAPGSVPTCDTVGVLGPAAALVGALQASETLKLLVGDRAARNPGLLSIDLWKVEIARIAVE
RRSDCPACGRGERAFLRQQVANRIVSLCGRDAVQVVVRPAVSLDLAALAERLSALGSLQHNPYLIRFMVEGYELTVFPDG
RAIVRGTTDPAIARVLYARYIGM

Sequences:

>Translated_343_residues
MTSDESAERHHRQILLPVIGRAGQSRIRAGRVCVLGLGALGSHTTMLLARAGVGFLRLVDRDVVEWTNLQRQALYEEEDV
RAALPKAVAAARAVQRIDQAIAVEPVVLDVDAASITGLIADVDVVVDGSDNFELRYLLNDAALELGRPWVYGGVIGTHGS
TMTIVPDETACLRCLFPDPPAPGSVPTCDTVGVLGPAAALVGALQASETLKLLVGDRAARNPGLLSIDLWKVEIARIAVE
RRSDCPACGRGERAFLRQQVANRIVSLCGRDAVQVVVRPAVSLDLAALAERLSALGSLQHNPYLIRFMVEGYELTVFPDG
RAIVRGTTDPAIARVLYARYIGM
>Mature_342_residues
TSDESAERHHRQILLPVIGRAGQSRIRAGRVCVLGLGALGSHTTMLLARAGVGFLRLVDRDVVEWTNLQRQALYEEEDVR
AALPKAVAAARAVQRIDQAIAVEPVVLDVDAASITGLIADVDVVVDGSDNFELRYLLNDAALELGRPWVYGGVIGTHGST
MTIVPDETACLRCLFPDPPAPGSVPTCDTVGVLGPAAALVGALQASETLKLLVGDRAARNPGLLSIDLWKVEIARIAVER
RSDCPACGRGERAFLRQQVANRIVSLCGRDAVQVVVRPAVSLDLAALAERLSALGSLQHNPYLIRFMVEGYELTVFPDGR
AIVRGTTDPAIARVLYARYIGM

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=243, Percent_Identity=38.6831275720165, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1787048, Length=252, Percent_Identity=34.5238095238095, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI87082356, Length=248, Percent_Identity=34.6774193548387, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17540406, Length=249, Percent_Identity=32.5301204819277, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321903, Length=253, Percent_Identity=32.0158102766798, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320598, Length=176, Percent_Identity=25.5681818181818, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325323, Length=171, Percent_Identity=30.9941520467836, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24582879, Length=215, Percent_Identity=36.7441860465116, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 36782; Mature: 36651

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDESAERHHRQILLPVIGRAGQSRIRAGRVCVLGLGALGSHTTMLLARAGVGFLRLVD
CCCCCHHHHHHHEEHHHHHCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHH
RDVVEWTNLQRQALYEEEDVRAALPKAVAAARAVQRIDQAIAVEPVVLDVDAASITGLIA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHEEE
DVDVVVDGSDNFELRYLLNDAALELGRPWVYGGVIGTHGSTMTIVPDETACLRCLFPDPP
EEEEEEECCCCEEEEEEECHHHHHHCCCEEEECEEECCCCEEEEECCCCCEEEEECCCCC
APGSVPTCDTVGVLGPAAALVGALQASETLKLLVGDRAARNPGLLSIDLWKVEIARIAVE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEHHHHHHHHHHHH
RRSDCPACGRGERAFLRQQVANRIVSLCGRDAVQVVVRPAVSLDLAALAERLSALGSLQH
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NPYLIRFMVEGYELTVFPDGRAIVRGTTDPAIARVLYARYIGM
CCEEEEEEEECEEEEEECCCCEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSDESAERHHRQILLPVIGRAGQSRIRAGRVCVLGLGALGSHTTMLLARAGVGFLRLVD
CCCCHHHHHHHEEHHHHHCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHH
RDVVEWTNLQRQALYEEEDVRAALPKAVAAARAVQRIDQAIAVEPVVLDVDAASITGLIA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHEEE
DVDVVVDGSDNFELRYLLNDAALELGRPWVYGGVIGTHGSTMTIVPDETACLRCLFPDPP
EEEEEEECCCCEEEEEEECHHHHHHCCCEEEECEEECCCCEEEEECCCCCEEEEECCCCC
APGSVPTCDTVGVLGPAAALVGALQASETLKLLVGDRAARNPGLLSIDLWKVEIARIAVE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEHHHHHHHHHHHH
RRSDCPACGRGERAFLRQQVANRIVSLCGRDAVQVVVRPAVSLDLAALAERLSALGSLQH
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NPYLIRFMVEGYELTVFPDGRAIVRGTTDPAIARVLYARYIGM
CCEEEEEEEECEEEEEECCCCEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]