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The map label for this gene is nuoI [H]

Identifier: 221633448

GI number: 221633448

Start: 1457747

End: 1458409

Strand: Reverse

Name: nuoI [H]

Synonym: trd_1470

Alternate gene names: 221633448

Gene position: 1458409-1457747 (Counterclockwise)

Preceding gene: 221633449

Following gene: 221633447

Centisome position: 72.81

GC content: 65.46

Gene sequence:

>663_bases
GTGGGCCCATCGCCTGGCCGGCAGCGCCAGGCGATCGAGCGCGCCAGACGCTGCGCATCGGTCAGAACGTTCCCGGCGGC
GGGTGGGCGGTCTGGTTGGGCAACGGGATCGCGCTCAGCGATGCCTGGCTGCGGTTGGACAGCGCGCCGCTGCGCGGCCG
GGTCGCCTTCCGAACGCCCTGACAGCGATCGCTCGAGGAGGCGCACGATGGTCTTACAGGACATTCGCGGCTTTCTGGTC
ACGCTGCGGCACTTTTTCCAGCGACCAGTGACGATTCAGTATCCCGAAGAGAAACGGGAGCCGGCACCGCGCTTCCGTGG
TTTGCCGGCGCTCCGCTCCGATCCCGAGACCGGTGAAGCGCTCTGTGTCGCCTGTGGGCTGTGCGCTCGCATTTGTCCGA
CGAGCTGCCTGGAGATGCACGTGGTCCCCTCGGAGGAGGGTGATCGGGAACTCGGCGAGTTCATTCTGCGTGCTGGTCGA
TGCATGTTCTGCGGGCTCTGTGCCCAGGTGTGCCCGGTCGATGCGATCACCATGAGTGGGGAATACGAGCAGTCGGTGCT
GGATCGCGAAGGACTCATCTACGTCAAGACAGAACTCGCAGCGATCGGCTCGCAGCGGCCGAGCTGGTGGGAAGCAGATG
GTGGAATGGGCTCCGAGCAATAG

Upstream 100 bases:

>100_bases
CTCGGCTATCGCTGGAACACGCGCCACGCGGTGCAGCTCTTCCAAGAAATCGCCCGCGCTGTGCCCGGCTATCGCGGCAT
CACGTTCCCGCGGCTCGAGC

Downstream 100 bases:

>100_bases
GGATGGGAGGCAGGGAACGATGGCGATCGAGACGGTACGGATCGAGCGCGATTCGCTCGGCGAGGTCGAGGTGCCGGCCG
AGGCGCTCTATGGCGCGCAG

Product: NADH-quinone oxidoreductase subunit i

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MGPSPGRQRQAIERARRCASVRTFPAAGGRSGWATGSRSAMPGCGWTARRCAAGSPSERPDSDRSRRRTMVLQDIRGFLV
TLRHFFQRPVTIQYPEEKREPAPRFRGLPALRSDPETGEALCVACGLCARICPTSCLEMHVVPSEEGDRELGEFILRAGR
CMFCGLCAQVCPVDAITMSGEYEQSVLDREGLIYVKTELAAIGSQRPSWWEADGGMGSEQ

Sequences:

>Translated_220_residues
MGPSPGRQRQAIERARRCASVRTFPAAGGRSGWATGSRSAMPGCGWTARRCAAGSPSERPDSDRSRRRTMVLQDIRGFLV
TLRHFFQRPVTIQYPEEKREPAPRFRGLPALRSDPETGEALCVACGLCARICPTSCLEMHVVPSEEGDRELGEFILRAGR
CMFCGLCAQVCPVDAITMSGEYEQSVLDREGLIYVKTELAAIGSQRPSWWEADGGMGSEQ
>Mature_219_residues
GPSPGRQRQAIERARRCASVRTFPAAGGRSGWATGSRSAMPGCGWTARRCAAGSPSERPDSDRSRRRTMVLQDIRGFLVT
LRHFFQRPVTIQYPEEKREPAPRFRGLPALRSDPETGEALCVACGLCARICPTSCLEMHVVPSEEGDRELGEFILRAGRC
MFCGLCAQVCPVDAITMSGEYEQSVLDREGLIYVKTELAAIGSQRPSWWEADGGMGSEQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=152, Percent_Identity=38.1578947368421, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI1788617, Length=129, Percent_Identity=36.4341085271318, Blast_Score=88, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17555194, Length=151, Percent_Identity=37.0860927152318, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17864306, Length=152, Percent_Identity=38.1578947368421, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 24171; Mature: 24040

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPSPGRQRQAIERARRCASVRTFPAAGGRSGWATGSRSAMPGCGWTARRCAAGSPSERP
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
DSDRSRRRTMVLQDIRGFLVTLRHFFQRPVTIQYPEEKREPAPRFRGLPALRSDPETGEA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCCHHHCCCCCCCCCCCCHHH
LCVACGLCARICPTSCLEMHVVPSEEGDRELGEFILRAGRCMFCGLCAQVCPVDAITMSG
HHHHHHHHHHHCHHHHHEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCEECCC
EYEQSVLDREGLIYVKTELAAIGSQRPSWWEADGGMGSEQ
CHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GPSPGRQRQAIERARRCASVRTFPAAGGRSGWATGSRSAMPGCGWTARRCAAGSPSERP
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
DSDRSRRRTMVLQDIRGFLVTLRHFFQRPVTIQYPEEKREPAPRFRGLPALRSDPETGEA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCCHHHCCCCCCCCCCCCHHH
LCVACGLCARICPTSCLEMHVVPSEEGDRELGEFILRAGRCMFCGLCAQVCPVDAITMSG
HHHHHHHHHHHCHHHHHEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCEECCC
EYEQSVLDREGLIYVKTELAAIGSQRPSWWEADGGMGSEQ
CHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA