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The map label for this gene is ansB [H]

Identifier: 221633447

GI number: 221633447

Start: 1456312

End: 1457727

Strand: Reverse

Name: ansB [H]

Synonym: trd_1469

Alternate gene names: 221633447

Gene position: 1457727-1456312 (Counterclockwise)

Preceding gene: 221633448

Following gene: 221633446

Centisome position: 72.78

GC content: 64.27

Gene sequence:

>1416_bases
ATGGCGATCGAGACGGTACGGATCGAGCGCGATTCGCTCGGCGAGGTCGAGGTGCCGGCCGAGGCGCTCTATGGCGCGCA
GACCGCCCGAGCGGTCCGCAATTTCCCGATAAGTGGCCTACGCCCGCATCCCGATCTCATCGTGGCGACCGCGCAGGTCA
AGCTCGCAGCGGCTCGCGTGAATCATCGTGCTGGCCGCTTGACCGACGAGCAGGCACGCGCGATCGAGCAGGCCGCGCAG
GAAATCATCGATGGCCTCTGGCACGAGCACTTCGTGGTCGATCCCTATCAGGCGGGTGCCGGGACATCGCACAACATGAA
TACCAACGAGGTCATCGCCAATCGGGCGAACGAGATCCTGGGCGGTCGACGCGGCGAGTACAAGCCAGTGCACCCCAACG
ACCACGTCAACCTCGGTCAATCGTCGAACGACGTCATCCCGACCGCGGGCCGTCTGGCCTTGTTGCGAGCCACTCCGAAG
CTGATCGAGGCATTCCGCAAGCTGGCCGATGCCTTCTGCCAGAAGGCGCAAGAGTTCGGGAATCTGACTAAAACTGGTCG
GACGCACCTGCAAGATGCGGTGCCGATCCGCTTGGGTGACGAGTTCACCGGATACGGGGCGGCCTTGCGGCGAGCAGCCG
ACCGGATCGCTGAGGCGCGCCTCGCGCTCACCGAGATCAATCTGGGAGCGACGGCACTGGGAACCGGGATCAACGCCTTC
CCCGGGTATCGCGCGCAAGTGGCGGAAGAGCTCAGTCGCATCACCGGTTTTCCCCTGCGCCCAGCGCGGAACACGTTCGA
GATCACCCAGAGTCATGCCGACTTCGCACACCTCTCGGGTGCCATCCGCAATGCTGCGCTCGAAGTGATCCGGATCGCCA
GCGATATCCGGCTGCTGGGCTCTGGGCCACGTGCGGGGTTGGGTGAGATCATCCTGCCACCAGTCCAGCCCGGTTCCTCC
ATCATGCCCGGCAAAGTGAACCCGTCGATTGCCGAGATGGTCAACATGGTCGCCTTCCAGGTGGTCGGGTACGATCACGC
AGTCTCGCTCGGTGTCCTCTCCGGGCAGCTCGATCTGAACGTCTGGACGCCGCTCATCGTGTACAACCTGCTCTGGGCGA
CCGATATTCTGACCAACGCTCTGGAGGTTTTCCGGACGCGCTGCGTGGAAGGGATTCAGGCCAACGAGAAACGGCTGGCC
GAGCTCGCGCATGGCAGCATCGCGCTGGCGACGATCTTGACGCCGCGGATCGGCTACCTGCGGGCGGCGGAGATCGCCAA
GAAGGCGTGGGAGACCGGTAAGACCGTGCGCGAGGTGGTCGTCGAGGAAGGAGTGCTTTCGCCCGAGGAGGCGGACGAGA
TTCTCGATCCGGTCAAGATGGCGCTGCCGCAGGATCCGGACGCGAACCAGTGCTGA

Upstream 100 bases:

>100_bases
GTCAAGACAGAACTCGCAGCGATCGGCTCGCAGCGGCCGAGCTGGTGGGAAGCAGATGGTGGAATGGGCTCCGAGCAATA
GGGATGGGAGGCAGGGAACG

Downstream 100 bases:

>100_bases
CGGCGCTGTCTTCGGATACAGTAGGAGCAGGCTGGTTGCTCCTGATCACCGAGGCAAGGTGTTCGTTGCCGGAGCGGCCG
CAGCGGTAGACAGCGGCATG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MAIETVRIERDSLGEVEVPAEALYGAQTARAVRNFPISGLRPHPDLIVATAQVKLAAARVNHRAGRLTDEQARAIEQAAQ
EIIDGLWHEHFVVDPYQAGAGTSHNMNTNEVIANRANEILGGRRGEYKPVHPNDHVNLGQSSNDVIPTAGRLALLRATPK
LIEAFRKLADAFCQKAQEFGNLTKTGRTHLQDAVPIRLGDEFTGYGAALRRAADRIAEARLALTEINLGATALGTGINAF
PGYRAQVAEELSRITGFPLRPARNTFEITQSHADFAHLSGAIRNAALEVIRIASDIRLLGSGPRAGLGEIILPPVQPGSS
IMPGKVNPSIAEMVNMVAFQVVGYDHAVSLGVLSGQLDLNVWTPLIVYNLLWATDILTNALEVFRTRCVEGIQANEKRLA
ELAHGSIALATILTPRIGYLRAAEIAKKAWETGKTVREVVVEEGVLSPEEADEILDPVKMALPQDPDANQC

Sequences:

>Translated_471_residues
MAIETVRIERDSLGEVEVPAEALYGAQTARAVRNFPISGLRPHPDLIVATAQVKLAAARVNHRAGRLTDEQARAIEQAAQ
EIIDGLWHEHFVVDPYQAGAGTSHNMNTNEVIANRANEILGGRRGEYKPVHPNDHVNLGQSSNDVIPTAGRLALLRATPK
LIEAFRKLADAFCQKAQEFGNLTKTGRTHLQDAVPIRLGDEFTGYGAALRRAADRIAEARLALTEINLGATALGTGINAF
PGYRAQVAEELSRITGFPLRPARNTFEITQSHADFAHLSGAIRNAALEVIRIASDIRLLGSGPRAGLGEIILPPVQPGSS
IMPGKVNPSIAEMVNMVAFQVVGYDHAVSLGVLSGQLDLNVWTPLIVYNLLWATDILTNALEVFRTRCVEGIQANEKRLA
ELAHGSIALATILTPRIGYLRAAEIAKKAWETGKTVREVVVEEGVLSPEEADEILDPVKMALPQDPDANQC
>Mature_470_residues
AIETVRIERDSLGEVEVPAEALYGAQTARAVRNFPISGLRPHPDLIVATAQVKLAAARVNHRAGRLTDEQARAIEQAAQE
IIDGLWHEHFVVDPYQAGAGTSHNMNTNEVIANRANEILGGRRGEYKPVHPNDHVNLGQSSNDVIPTAGRLALLRATPKL
IEAFRKLADAFCQKAQEFGNLTKTGRTHLQDAVPIRLGDEFTGYGAALRRAADRIAEARLALTEINLGATALGTGINAFP
GYRAQVAEELSRITGFPLRPARNTFEITQSHADFAHLSGAIRNAALEVIRIASDIRLLGSGPRAGLGEIILPPVQPGSSI
MPGKVNPSIAEMVNMVAFQVVGYDHAVSLGVLSGQLDLNVWTPLIVYNLLWATDILTNALEVFRTRCVEGIQANEKRLAE
LAHGSIALATILTPRIGYLRAAEIAKKAWETGKTVREVVVEEGVLSPEEADEILDPVKMALPQDPDANQC

Specific function: Tricarboxylic acid cycle [C]

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=464, Percent_Identity=45.9051724137931, Blast_Score=384, Evalue=1e-107,
Organism=Escherichia coli, GI1787896, Length=459, Percent_Identity=44.2265795206972, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI87082375, Length=461, Percent_Identity=43.1670281995662, Blast_Score=355, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI17553882, Length=463, Percent_Identity=46.6522678185745, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI32565146, Length=328, Percent_Identity=42.9878048780488, Blast_Score=263, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324993, Length=464, Percent_Identity=44.1810344827586, Blast_Score=382, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24640179, Length=469, Percent_Identity=43.2835820895522, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640177, Length=469, Percent_Identity=43.0703624733475, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=42.6724137931034, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24662684, Length=466, Percent_Identity=42.4892703862661, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24583245, Length=470, Percent_Identity=39.3617021276596, Blast_Score=336, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 50911; Mature: 50779

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIETVRIERDSLGEVEVPAEALYGAQTARAVRNFPISGLRPHPDLIVATAQVKLAAARV
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHH
NHRAGRLTDEQARAIEQAAQEIIDGLWHEHFVVDPYQAGAGTSHNMNTNEVIANRANEIL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCHHCCCCCCCCCCHHHHHHHHHHHHH
GGRRGEYKPVHPNDHVNLGQSSNDVIPTAGRLALLRATPKLIEAFRKLADAFCQKAQEFG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
NLTKTGRTHLQDAVPIRLGDEFTGYGAALRRAADRIAEARLALTEINLGATALGTGINAF
CHHHHCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHCCCCCCC
PGYRAQVAEELSRITGFPLRPARNTFEITQSHADFAHLSGAIRNAALEVIRIASDIRLLG
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
SGPRAGLGEIILPPVQPGSSIMPGKVNPSIAEMVNMVAFQVVGYDHAVSLGVLSGQLDLN
CCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHEEEECCCCCH
VWTPLIVYNLLWATDILTNALEVFRTRCVEGIQANEKRLAELAHGSIALATILTPRIGYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCCHHHH
RAAEIAKKAWETGKTVREVVVEEGVLSPEEADEILDPVKMALPQDPDANQC
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AIETVRIERDSLGEVEVPAEALYGAQTARAVRNFPISGLRPHPDLIVATAQVKLAAARV
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHH
NHRAGRLTDEQARAIEQAAQEIIDGLWHEHFVVDPYQAGAGTSHNMNTNEVIANRANEIL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCHHCCCCCCCCCCHHHHHHHHHHHHH
GGRRGEYKPVHPNDHVNLGQSSNDVIPTAGRLALLRATPKLIEAFRKLADAFCQKAQEFG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
NLTKTGRTHLQDAVPIRLGDEFTGYGAALRRAADRIAEARLALTEINLGATALGTGINAF
CHHHHCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHCCCCCCC
PGYRAQVAEELSRITGFPLRPARNTFEITQSHADFAHLSGAIRNAALEVIRIASDIRLLG
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
SGPRAGLGEIILPPVQPGSSIMPGKVNPSIAEMVNMVAFQVVGYDHAVSLGVLSGQLDLN
CCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHEEEECCCCCH
VWTPLIVYNLLWATDILTNALEVFRTRCVEGIQANEKRLAELAHGSIALATILTPRIGYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCCHHHH
RAAEIAKKAWETGKTVREVVVEEGVLSPEEADEILDPVKMALPQDPDANQC
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]