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The map label for this gene is amt [H]

Identifier: 221633091

GI number: 221633091

Start: 1085857

End: 1087149

Strand: Reverse

Name: amt [H]

Synonym: trd_1107

Alternate gene names: 221633091

Gene position: 1087149-1085857 (Counterclockwise)

Preceding gene: 221633092

Following gene: 221633090

Centisome position: 54.28

GC content: 64.04

Gene sequence:

>1293_bases
ATGAGCGAGGGGGCGATCGCCTGGATGCTGGTTGCGACAGCATTGGTCCTCCTGATGACCCCGGCATTAGGGTTTTTCTA
TGGGGGGCTCGTTCGCTCCAAGAACGTTCTCAACACGCTGATGATGAGTTTCGGCGCGGTATCTGTCGTAGGGCTCGCGT
GGGCGTTGCTCGGCTACTCGCTCGCCTTTTCCGCCGGGTGGCCGCTGGTCGGGGGCCTTTCGCACGCGCTGTTGCGTGGG
GTCGACCTAGAAGCGTCCGGCGCGATCCCACACGTGCTGTTCATGGCCTTCCAGGGGACTTTCGCCGTCATCACCGCCGC
ACTCATTTCAGGGGCGCTCGTAGAACGCATGCGCTTTGGCCCTTACCTCGCATTCCTCGGTCTCTGGGCGATCGCGATCT
ATGCACCGATCGCGCATTGGATTTGGGGAGGTGGCTGGCTCGCTGACCTGGGGGCCCTCGATTTTGCAGGTGGAACTGTG
GTGCATATCAATGCTGGTATCGCGGCCGTCGTGGCTGCACTCGTCCTCGGCCGCCGACGCGATTACGGTCGTCAGGCTAT
TCTTCCGCACAATATTCCGTTCGTCTTGCTGGGTGCGATGCTGCTGTGGCTCGGCTGGTTCGGGTTCAACGCGGGGAGTG
CACTCGGGGCGAACGAGATCGCGGGACTCGCTTTCGCGAACACCATGCTGGCGCCGATGGCAACCGCGCTGGTGTGGATG
ATTCTCGACCTCGTGCGGAGCGGCAAGGCGACGGCCGTAGGACTCGCGACGGCGATCGTCGTCGGTCTCGTCGCCATCAC
TCCAGCTGCGGGATTCGTCTCGCCATTGTCGGCGATCGTACTCGGGGCGCTCGCTGCCTTTCCGAGTTATTTCGCGTTGC
TGTGGCGAGCCCGGACGCGACTCGACGATTCGCTCGATGTCTTCGCGGCACACGGTGTCGGTGGGCTGACCGGAGCATTG
TTGACCGGTGTCTTCGCGCAGGAGTCGTGGAACGGAGTCGCGAACGGGCTGCTCTTCGGGAACCCGCTGCAACTGCTGAT
CCAAGCGGTCGCTGTGCTTGCGGTCGTCGCCTATAGTGCAGCTGGCACGTTCCTCATCTTGCGAGCGATCGCACTGGTGA
CGTCGCTCCGCGTTGCGGATGCTGCCGAGCGTGCCGGCTTGGACGTCACCGAGCACGGCGAGCAGGCCTACACGAGTGGA
GAGGGGGCGGTGTTGCTCTTGCCGCTGAGCCGGACGGGCGAGGTGACGGCGCGACCTGGGCCGGTCGTCGAAAAGGTGGG
GGGCAGCGCATGA

Upstream 100 bases:

>100_bases
ATTCGGCGACAGCGCGGACCACCATTGGCGCATCGTATCGATGAGGAGAGGCGGGAGATTCGTCGGTCGCTGCGCTGAGG
CAGCAGGAAGGAGCTCGACG

Downstream 100 bases:

>100_bases
AGCTCATCGTCGCCATCATTCGACCAGAGAAGCTCAACGAGGTTCTGGAAGCGTTGTTCCGTGCAGAGGTGCGTGGGCTG
ACGATTTCGGCTGCCCAAGG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSEGAIAWMLVATALVLLMTPALGFFYGGLVRSKNVLNTLMMSFGAVSVVGLAWALLGYSLAFSAGWPLVGGLSHALLRG
VDLEASGAIPHVLFMAFQGTFAVITAALISGALVERMRFGPYLAFLGLWAIAIYAPIAHWIWGGGWLADLGALDFAGGTV
VHINAGIAAVVAALVLGRRRDYGRQAILPHNIPFVLLGAMLLWLGWFGFNAGSALGANEIAGLAFANTMLAPMATALVWM
ILDLVRSGKATAVGLATAIVVGLVAITPAAGFVSPLSAIVLGALAAFPSYFALLWRARTRLDDSLDVFAAHGVGGLTGAL
LTGVFAQESWNGVANGLLFGNPLQLLIQAVAVLAVVAYSAAGTFLILRAIALVTSLRVADAAERAGLDVTEHGEQAYTSG
EGAVLLLPLSRTGEVTARPGPVVEKVGGSA

Sequences:

>Translated_430_residues
MSEGAIAWMLVATALVLLMTPALGFFYGGLVRSKNVLNTLMMSFGAVSVVGLAWALLGYSLAFSAGWPLVGGLSHALLRG
VDLEASGAIPHVLFMAFQGTFAVITAALISGALVERMRFGPYLAFLGLWAIAIYAPIAHWIWGGGWLADLGALDFAGGTV
VHINAGIAAVVAALVLGRRRDYGRQAILPHNIPFVLLGAMLLWLGWFGFNAGSALGANEIAGLAFANTMLAPMATALVWM
ILDLVRSGKATAVGLATAIVVGLVAITPAAGFVSPLSAIVLGALAAFPSYFALLWRARTRLDDSLDVFAAHGVGGLTGAL
LTGVFAQESWNGVANGLLFGNPLQLLIQAVAVLAVVAYSAAGTFLILRAIALVTSLRVADAAERAGLDVTEHGEQAYTSG
EGAVLLLPLSRTGEVTARPGPVVEKVGGSA
>Mature_429_residues
SEGAIAWMLVATALVLLMTPALGFFYGGLVRSKNVLNTLMMSFGAVSVVGLAWALLGYSLAFSAGWPLVGGLSHALLRGV
DLEASGAIPHVLFMAFQGTFAVITAALISGALVERMRFGPYLAFLGLWAIAIYAPIAHWIWGGGWLADLGALDFAGGTVV
HINAGIAAVVAALVLGRRRDYGRQAILPHNIPFVLLGAMLLWLGWFGFNAGSALGANEIAGLAFANTMLAPMATALVWMI
LDLVRSGKATAVGLATAIVVGLVAITPAAGFVSPLSAIVLGALAAFPSYFALLWRARTRLDDSLDVFAAHGVGGLTGALL
TGVFAQESWNGVANGLLFGNPLQLLIQAVAVLAVVAYSAAGTFLILRAIALVTSLRVADAAERAGLDVTEHGEQAYTSGE
GAVLLLPLSRTGEVTARPGPVVEKVGGSA

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=398, Percent_Identity=44.4723618090452, Blast_Score=314, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI17550180, Length=437, Percent_Identity=29.2906178489703, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17550182, Length=441, Percent_Identity=30.3854875283447, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32564042, Length=441, Percent_Identity=28.7981859410431, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI133931100, Length=432, Percent_Identity=27.3148148148148, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6324187, Length=442, Percent_Identity=36.4253393665158, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6325396, Length=415, Percent_Identity=34.6987951807229, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6321559, Length=412, Percent_Identity=33.7378640776699, Blast_Score=220, Evalue=3e-58,
Organism=Drosophila melanogaster, GI161078314, Length=402, Percent_Identity=29.8507462686567, Blast_Score=128, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44315; Mature: 44183

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEGAIAWMLVATALVLLMTPALGFFYGGLVRSKNVLNTLMMSFGAVSVVGLAWALLGYS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFSAGWPLVGGLSHALLRGVDLEASGAIPHVLFMAFQGTFAVITAALISGALVERMRFG
HHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
PYLAFLGLWAIAIYAPIAHWIWGGGWLADLGALDFAGGTVVHINAGIAAVVAALVLGRRR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCC
DYGRQAILPHNIPFVLLGAMLLWLGWFGFNAGSALGANEIAGLAFANTMLAPMATALVWM
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ILDLVRSGKATAVGLATAIVVGLVAITPAAGFVSPLSAIVLGALAAFPSYFALLWRARTR
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDDSLDVFAAHGVGGLTGALLTGVFAQESWNGVANGLLFGNPLQLLIQAVAVLAVVAYSA
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AGTFLILRAIALVTSLRVADAAERAGLDVTEHGEQAYTSGEGAVLLLPLSRTGEVTARPG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHCCCCCCEEEEECCCCCCCCCCCC
PVVEKVGGSA
CHHHHCCCCC
>Mature Secondary Structure 
SEGAIAWMLVATALVLLMTPALGFFYGGLVRSKNVLNTLMMSFGAVSVVGLAWALLGYS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFSAGWPLVGGLSHALLRGVDLEASGAIPHVLFMAFQGTFAVITAALISGALVERMRFG
HHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
PYLAFLGLWAIAIYAPIAHWIWGGGWLADLGALDFAGGTVVHINAGIAAVVAALVLGRRR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCC
DYGRQAILPHNIPFVLLGAMLLWLGWFGFNAGSALGANEIAGLAFANTMLAPMATALVWM
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ILDLVRSGKATAVGLATAIVVGLVAITPAAGFVSPLSAIVLGALAAFPSYFALLWRARTR
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDDSLDVFAAHGVGGLTGALLTGVFAQESWNGVANGLLFGNPLQLLIQAVAVLAVVAYSA
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AGTFLILRAIALVTSLRVADAAERAGLDVTEHGEQAYTSGEGAVLLLPLSRTGEVTARPG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHCCCCCCEEEEECCCCCCCCCCCC
PVVEKVGGSA
CHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]