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The map label for this gene is rnhB [H]

Identifier: 221633032

GI number: 221633032

Start: 1016707

End: 1017366

Strand: Reverse

Name: rnhB [H]

Synonym: trd_1047

Alternate gene names: 221633032

Gene position: 1017366-1016707 (Counterclockwise)

Preceding gene: 221633033

Following gene: 221633031

Centisome position: 50.79

GC content: 63.79

Gene sequence:

>660_bases
ATGGTCTCGCTCGCCTCGACTCGTCAGCGGCGTTCGGGCCGAGGCGAGCGACCGACGTTCGAACTCGAGCAAGCTCTTTG
GCAAGCGGGCAACCGCTGGGTCGCTGGCGTGGACGAGGTCGGGCGCGGAGCGCTGGCTGGTCCGGTCGTGGCGGCGGCCT
GTATCTTCGAGCCGTCGACTGAGTTACCCGATCAGTTGCGGGATTCGAAGCGGTTGACCGATCCCCAACGACGAGCGCTG
GCTGATTGGGTCCGACAGTATTCGCTGCGCTGGGCCATCGGTGCAGCGTCGCATCGCGAGGTCGACCGACTGGGTATCGT
CCGGGCGACCGCTCTCGCCATGCTGAGGGCGCTCCAGCGTCTCGGTCCTGTCGATCATGTCCTCTACGATGGCTTGCCAC
TGACCGAGTTCGAGCGACTACCGGGAACGGCGGTCGTGCATGGCGACATGCGCTGTGCATCGATCGCCGCTGCATCGATT
TTGGCAAAGGTGATCCGCGACGATCTCATGGCCCGTCTCGCAGTCCGGTATCCTGGCTATGGTTGGGAAGAAAATGCTGG
ATACGGCACACCGTCGCATCGACGTGCGATTCGCGAGCTCGGCTCGACGCCTTTCCACCGGCGATCGTTCCAAGTAAGGA
TGGACACGGACGAGGAGTAG

Upstream 100 bases:

>100_bases
GCGCGCGGCTTTACTACCTGCGTGAGCGAGCAGGCAAGGCGGCTCGCCTGAAGGAGCGCCGTCGCCCCGAAGGTAGCTAC
CTGCGCTGAGGAGCGGAACG

Downstream 100 bases:

>100_bases
TCGGTGGGACGGCAGTGCCTGGGCGAGGCGGGCGAGCGAGCGGCAGCGCGATGGCTCGAAGAGGCGGGTTGGCATGTCCT
GGCTCGCAACTGGCGGTGTC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MVSLASTRQRRSGRGERPTFELEQALWQAGNRWVAGVDEVGRGALAGPVVAAACIFEPSTELPDQLRDSKRLTDPQRRAL
ADWVRQYSLRWAIGAASHREVDRLGIVRATALAMLRALQRLGPVDHVLYDGLPLTEFERLPGTAVVHGDMRCASIAAASI
LAKVIRDDLMARLAVRYPGYGWEENAGYGTPSHRRAIRELGSTPFHRRSFQVRMDTDEE

Sequences:

>Translated_219_residues
MVSLASTRQRRSGRGERPTFELEQALWQAGNRWVAGVDEVGRGALAGPVVAAACIFEPSTELPDQLRDSKRLTDPQRRAL
ADWVRQYSLRWAIGAASHREVDRLGIVRATALAMLRALQRLGPVDHVLYDGLPLTEFERLPGTAVVHGDMRCASIAAASI
LAKVIRDDLMARLAVRYPGYGWEENAGYGTPSHRRAIRELGSTPFHRRSFQVRMDTDEE
>Mature_219_residues
MVSLASTRQRRSGRGERPTFELEQALWQAGNRWVAGVDEVGRGALAGPVVAAACIFEPSTELPDQLRDSKRLTDPQRRAL
ADWVRQYSLRWAIGAASHREVDRLGIVRATALAMLRALQRLGPVDHVLYDGLPLTEFERLPGTAVVHGDMRCASIAAASI
LAKVIRDDLMARLAVRYPGYGWEENAGYGTPSHRRAIRELGSTPFHRRSFQVRMDTDEE

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=182, Percent_Identity=45.0549450549451, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 24401; Mature: 24401

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLASTRQRRSGRGERPTFELEQALWQAGNRWVAGVDEVGRGALAGPVVAAACIFEPST
CCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEECHHHHCCCCHHHHHHHHHHHCCCCC
ELPDQLRDSKRLTDPQRRALADWVRQYSLRWAIGAASHREVDRLGIVRATALAMLRALQR
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LGPVDHVLYDGLPLTEFERLPGTAVVHGDMRCASIAAASILAKVIRDDLMARLAVRYPGY
HCCHHHHHHCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GWEENAGYGTPSHRRAIRELGSTPFHRRSFQVRMDTDEE
CCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MVSLASTRQRRSGRGERPTFELEQALWQAGNRWVAGVDEVGRGALAGPVVAAACIFEPST
CCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEECHHHHCCCCHHHHHHHHHHHCCCCC
ELPDQLRDSKRLTDPQRRALADWVRQYSLRWAIGAASHREVDRLGIVRATALAMLRALQR
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LGPVDHVLYDGLPLTEFERLPGTAVVHGDMRCASIAAASILAKVIRDDLMARLAVRYPGY
HCCHHHHHHCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GWEENAGYGTPSHRRAIRELGSTPFHRRSFQVRMDTDEE
CCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA