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The map label for this gene is hisS [H]

Identifier: 221633028

GI number: 221633028

Start: 1013222

End: 1014610

Strand: Reverse

Name: hisS [H]

Synonym: trd_1043

Alternate gene names: 221633028

Gene position: 1014610-1013222 (Counterclockwise)

Preceding gene: 221633029

Following gene: 221633027

Centisome position: 50.65

GC content: 62.71

Gene sequence:

>1389_bases
GTGAGAAAGAGGTCGGTAACGATGGGCGACGCAGCAGTACGGCGACCACCGCAAGTGCTGCGGGGAATGCGCGACTTCAC
TCCGCGGCAGATGCTTCTGCGGCAACGAGTCATGGAGGTCCTTCGCCAAGTCTTCGAACGATACGGGTACGACCCGATCG
ATACGCCGCTGCTCGAGTATTACGAAGTCCTTTCAGGAAAGTATGGGGAAGAAGGAGAAAAGCTCCTCTACCGCTTCGTC
GATCAGGGCGGTCGAGAGGTCGGGCTGCGCTACGACCTGACTGTTCCGCTGGCACGGTACGTGGCAGTTCATCGCGCTGA
GTTGACGTTCCCGTTCAAGCGTTACCACATTGGGCCGGTCTTCCGCGCTGAACGGCCACAACGCGGACGGTTTCGCCAGT
TCTGGCAGTGCGATGTCGACGTGGTCGGCACTCGATCGATGATGGCCGATGCAGAAGTACTGTCGGTCTGGATCGATGCG
CTCGGCGCCTTGCGTATTCCCAACGCCGTGATCCACGTCAACCATCGCCAACTCCTGGAGGCTCTCGTCAGAGCGGCTGG
CGTGGCGCCGAGTCTCGCGCTCGGTGTGTTTCGTGCCATCGACAAGCTGGCGAAGATCGGGCTCGACGGTGTACGCGAGG
AACTCCTCCGGATCGGTGTCGAGGCACCGGCGGCTGAGCGGGTGCTGGCGGCGATCGCGCTGCGTGGTGAGCCGGAGTCG
ATCATCCCGCAGCTGCGTGTGATGTTTGCGGGTGATGAGGCAGCTGAACGGGCTGTCAGCGAACTGGCTGAGCTTTTCCG
CTATCTCGAATGGTTCGGGGTGCGACCAGGGAGTGCGATCGTCGATCTCTCGCTCGCCCGGGGGCTGGATTACTATACCG
GACCCGTGGCAGAAGCGATCGTCAGCGAACCAGCGATCGGTTCACTGGGGGGCGCAGGGCGCTATGATCACTTGATCGGA
CTCTTCTTGGGTGAGGAAATTCCCGCGACTGGGGCATCACTCGGCCTCGAGCGCCTGGTAGAGGTCGTCGACGAGTACGG
TTTGCTGCCGACGCCGGCAAGCGTGACGGAGGTGCTGGTCGTTTCGGTCGAAGCAGACCATCGAGCGCAGGCGCTGCGCA
TCGCCCGCGAACTCCGGCAGGCAGGACTCGACACCGAGGTCTATCTGGGTGAGCGGATCGACCTCCGTCGTCAACTCCAA
TATGCCGATCGCAAGGGTGTGCCGGTCGCGGTCATCATCGGGCCGGACGAGATAGCCAATGACATGGTGACAGTGCGCGA
CCTTCGCACGGGAGAGCAAGTTCGAGTTCCACGCGCGGAACTCGTGGCTGCGGTGCGGAAACCGCTGGGCGCCGCTCGTC
AGGGCGAGAGGGGCAGTGCGGATGAGTGA

Upstream 100 bases:

>100_bases
GGATCGTCATCGCCATGCCAGGCTCGACCGGTGCTGTGCGATTGGCCATGGATGAACTCGTTCTTCCCGAACTCGCGCAC
CTCGTCTTCGAGGCACGCAA

Downstream 100 bases:

>100_bases
CTTGACACCGCATCACCGCATCATGGTCGTGGTGGCTCATCCTGACGACATGGAATTCGGGGCTGGTGGAACGGTTGCCC
GCTTGGCCCGGGAGGGAAAG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MRKRSVTMGDAAVRRPPQVLRGMRDFTPRQMLLRQRVMEVLRQVFERYGYDPIDTPLLEYYEVLSGKYGEEGEKLLYRFV
DQGGREVGLRYDLTVPLARYVAVHRAELTFPFKRYHIGPVFRAERPQRGRFRQFWQCDVDVVGTRSMMADAEVLSVWIDA
LGALRIPNAVIHVNHRQLLEALVRAAGVAPSLALGVFRAIDKLAKIGLDGVREELLRIGVEAPAAERVLAAIALRGEPES
IIPQLRVMFAGDEAAERAVSELAELFRYLEWFGVRPGSAIVDLSLARGLDYYTGPVAEAIVSEPAIGSLGGAGRYDHLIG
LFLGEEIPATGASLGLERLVEVVDEYGLLPTPASVTEVLVVSVEADHRAQALRIARELRQAGLDTEVYLGERIDLRRQLQ
YADRKGVPVAVIIGPDEIANDMVTVRDLRTGEQVRVPRAELVAAVRKPLGAARQGERGSADE

Sequences:

>Translated_462_residues
MRKRSVTMGDAAVRRPPQVLRGMRDFTPRQMLLRQRVMEVLRQVFERYGYDPIDTPLLEYYEVLSGKYGEEGEKLLYRFV
DQGGREVGLRYDLTVPLARYVAVHRAELTFPFKRYHIGPVFRAERPQRGRFRQFWQCDVDVVGTRSMMADAEVLSVWIDA
LGALRIPNAVIHVNHRQLLEALVRAAGVAPSLALGVFRAIDKLAKIGLDGVREELLRIGVEAPAAERVLAAIALRGEPES
IIPQLRVMFAGDEAAERAVSELAELFRYLEWFGVRPGSAIVDLSLARGLDYYTGPVAEAIVSEPAIGSLGGAGRYDHLIG
LFLGEEIPATGASLGLERLVEVVDEYGLLPTPASVTEVLVVSVEADHRAQALRIARELRQAGLDTEVYLGERIDLRRQLQ
YADRKGVPVAVIIGPDEIANDMVTVRDLRTGEQVRVPRAELVAAVRKPLGAARQGERGSADE
>Mature_462_residues
MRKRSVTMGDAAVRRPPQVLRGMRDFTPRQMLLRQRVMEVLRQVFERYGYDPIDTPLLEYYEVLSGKYGEEGEKLLYRFV
DQGGREVGLRYDLTVPLARYVAVHRAELTFPFKRYHIGPVFRAERPQRGRFRQFWQCDVDVVGTRSMMADAEVLSVWIDA
LGALRIPNAVIHVNHRQLLEALVRAAGVAPSLALGVFRAIDKLAKIGLDGVREELLRIGVEAPAAERVLAAIALRGEPES
IIPQLRVMFAGDEAAERAVSELAELFRYLEWFGVRPGSAIVDLSLARGLDYYTGPVAEAIVSEPAIGSLGGAGRYDHLIG
LFLGEEIPATGASLGLERLVEVVDEYGLLPTPASVTEVLVVSVEADHRAQALRIARELRQAGLDTEVYLGERIDLRRQLQ
YADRKGVPVAVIIGPDEIANDMVTVRDLRTGEQVRVPRAELVAAVRKPLGAARQGERGSADE

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=450, Percent_Identity=36.6666666666667, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI15029520, Length=457, Percent_Identity=33.9168490153173, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI65287717, Length=476, Percent_Identity=25.2100840336134, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1788861, Length=447, Percent_Identity=30.6487695749441, Blast_Score=155, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI71993693, Length=460, Percent_Identity=32.8260869565217, Blast_Score=247, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI71993686, Length=460, Percent_Identity=32.8260869565217, Blast_Score=247, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6325290, Length=466, Percent_Identity=31.9742489270386, Blast_Score=243, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24643061, Length=475, Percent_Identity=36.6315789473684, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24643059, Length=494, Percent_Identity=35.2226720647773, Blast_Score=257, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 51263; Mature: 51263

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRSVTMGDAAVRRPPQVLRGMRDFTPRQMLLRQRVMEVLRQVFERYGYDPIDTPLLEY
CCCCCCCCCCHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
YEVLSGKYGEEGEKLLYRFVDQGGREVGLRYDLTVPLARYVAVHRAELTFPFKRYHIGPV
HHHHCCCCCCHHHHHHHHHHHCCCCEECCEEECHHHHHHHHHHHHHHCCCCHHHHCCCCC
FRAERPQRGRFRQFWQCDVDVVGTRSMMADAEVLSVWIDALGALRIPNAVIHVNHRQLLE
CCCCCCCCCHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHH
ALVRAAGVAPSLALGVFRAIDKLAKIGLDGVREELLRIGVEAPAAERVLAAIALRGEPES
HHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHH
IIPQLRVMFAGDEAAERAVSELAELFRYLEWFGVRPGSAIVDLSLARGLDYYTGPVAEAI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEHHHHHCCCHHCCHHHHHH
VSEPAIGSLGGAGRYDHLIGLFLGEEIPATGASLGLERLVEVVDEYGLLPTPASVTEVLV
HCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHEEEE
VSVEADHRAQALRIARELRQAGLDTEVYLGERIDLRRQLQYADRKGVPVAVIIGPDEIAN
EEECCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCHHHHC
DMVTVRDLRTGEQVRVPRAELVAAVRKPLGAARQGERGSADE
CCEEHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRKRSVTMGDAAVRRPPQVLRGMRDFTPRQMLLRQRVMEVLRQVFERYGYDPIDTPLLEY
CCCCCCCCCCHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
YEVLSGKYGEEGEKLLYRFVDQGGREVGLRYDLTVPLARYVAVHRAELTFPFKRYHIGPV
HHHHCCCCCCHHHHHHHHHHHCCCCEECCEEECHHHHHHHHHHHHHHCCCCHHHHCCCCC
FRAERPQRGRFRQFWQCDVDVVGTRSMMADAEVLSVWIDALGALRIPNAVIHVNHRQLLE
CCCCCCCCCHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHH
ALVRAAGVAPSLALGVFRAIDKLAKIGLDGVREELLRIGVEAPAAERVLAAIALRGEPES
HHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHH
IIPQLRVMFAGDEAAERAVSELAELFRYLEWFGVRPGSAIVDLSLARGLDYYTGPVAEAI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEHHHHHCCCHHCCHHHHHH
VSEPAIGSLGGAGRYDHLIGLFLGEEIPATGASLGLERLVEVVDEYGLLPTPASVTEVLV
HCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHEEEE
VSVEADHRAQALRIARELRQAGLDTEVYLGERIDLRRQLQYADRKGVPVAVIIGPDEIAN
EEECCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCHHHHC
DMVTVRDLRTGEQVRVPRAELVAAVRKPLGAARQGERGSADE
CCEEHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]