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The map label for this gene is truB [H]

Identifier: 221632777

GI number: 221632777

Start: 758252

End: 759160

Strand: Reverse

Name: truB [H]

Synonym: trd_0770

Alternate gene names: 221632777

Gene position: 759160-758252 (Counterclockwise)

Preceding gene: 221632778

Following gene: 221632776

Centisome position: 37.9

GC content: 62.6

Gene sequence:

>909_bases
GTGCACGGCATACTCCTGGTGGACAAGCCACGCGGTTGGACATCCCACGACGTGGTGCAATGGGTCCGCACGCGCCTCCG
CGTTCAAACGGTCGGTCACGGCGGCACGCTCGACCCGGCCGCCGAAGGGTTGCTCGTTATCGCTGTCGGTATCGCCACGC
GCCTCGTCCGACACGCAGCCGATGCGGACAAAAGCTATGTCGCACACATTGTCCTCGGTACCGGGACGACGAGCGACGAT
CTCGAAGGTGAACCGCTGGCAGCCTCACCGCTATCCCTGTACCGACCCTCGCTCCGAGACGTCCAGTCGGCACTCGCCGA
GTTCCTCGGCGAATACGAACAACAGCCGCCGATCTATTCGGCACTGAAAGTTGGCGGCGTCGCAGCCTACCGGCGTGCCC
GGGCTGGTCAGCCAGTGCCGCTCAAACGACGCGTGGTGCGAGTCAACAGCCTACGTCTCGTGCAGTATTCGTATCCGGAT
CTCATCGTCTCGCTCGACTGTGGGAAGGGATTCTATGTCCGTTCCTTTGCTCGCGACCTCGGTACCCGCTTGGGCACTGG
CGCTTACCTGCACGGCCTCGTCCGCACGCGCGTGGGCCGATTTTCGCTCGCTTCCGCCTGGTCGATCGACGAGTTGAATC
GTGTGCTCACACCATCATCCTGGCCATTGATCGCTCGTCACCCGGATGAGTTCGTTGCGGAACTCCCCGTTTTGTTGGTT
CCTGAACGGGAACTTCGAGCATGGTATCATGGCGCGCCGGTGCGCTGTTCAGCGAGCAGCTCACCTGGCACCACTGCTCG
CGCCTACGCCCCAGATGGGAACTGGATCGGACTCGCTCATTTCGACCCACAAAAGCGATACTGGCAGCCGGTCCTCGTGC
ATCGCGACGGGTCTGGATGGAGAGGCTAG

Upstream 100 bases:

>100_bases
CCTTCCCCCGGGCGAAGCTGCCCGACGATCGTTTCTCGACGACCTCGCAGCACAAGTGAGCGCACTACTCAGCATCGAGC
TTCCATCGGGGTGAGCAACA

Downstream 100 bases:

>100_bases
ACGCCCAATGAGGATCATTCGCTCCTGGAGTGACATTCCGCCTGAGCCTCATGTCGTGACCATCGGCAACTTCGATGGTG
TCCACCGTGGTCACCAGTAC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MHGILLVDKPRGWTSHDVVQWVRTRLRVQTVGHGGTLDPAAEGLLVIAVGIATRLVRHAADADKSYVAHIVLGTGTTSDD
LEGEPLAASPLSLYRPSLRDVQSALAEFLGEYEQQPPIYSALKVGGVAAYRRARAGQPVPLKRRVVRVNSLRLVQYSYPD
LIVSLDCGKGFYVRSFARDLGTRLGTGAYLHGLVRTRVGRFSLASAWSIDELNRVLTPSSWPLIARHPDEFVAELPVLLV
PERELRAWYHGAPVRCSASSSPGTTARAYAPDGNWIGLAHFDPQKRYWQPVLVHRDGSGWRG

Sequences:

>Translated_302_residues
MHGILLVDKPRGWTSHDVVQWVRTRLRVQTVGHGGTLDPAAEGLLVIAVGIATRLVRHAADADKSYVAHIVLGTGTTSDD
LEGEPLAASPLSLYRPSLRDVQSALAEFLGEYEQQPPIYSALKVGGVAAYRRARAGQPVPLKRRVVRVNSLRLVQYSYPD
LIVSLDCGKGFYVRSFARDLGTRLGTGAYLHGLVRTRVGRFSLASAWSIDELNRVLTPSSWPLIARHPDEFVAELPVLLV
PERELRAWYHGAPVRCSASSSPGTTARAYAPDGNWIGLAHFDPQKRYWQPVLVHRDGSGWRG
>Mature_302_residues
MHGILLVDKPRGWTSHDVVQWVRTRLRVQTVGHGGTLDPAAEGLLVIAVGIATRLVRHAADADKSYVAHIVLGTGTTSDD
LEGEPLAASPLSLYRPSLRDVQSALAEFLGEYEQQPPIYSALKVGGVAAYRRARAGQPVPLKRRVVRVNSLRLVQYSYPD
LIVSLDCGKGFYVRSFARDLGTRLGTGAYLHGLVRTRVGRFSLASAWSIDELNRVLTPSSWPLIARHPDEFVAELPVLLV
PERELRAWYHGAPVRCSASSSPGTTARAYAPDGNWIGLAHFDPQKRYWQPVLVHRDGSGWRG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=239, Percent_Identity=30.9623430962343, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI215599015, Length=206, Percent_Identity=31.0679611650485, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4503337, Length=203, Percent_Identity=31.0344827586207, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI2367200, Length=296, Percent_Identity=30.7432432432432, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17553978, Length=199, Percent_Identity=29.6482412060301, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=178, Percent_Identity=32.5842696629214, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=211, Percent_Identity=28.9099526066351, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281364189, Length=199, Percent_Identity=29.6482412060301, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364187, Length=199, Percent_Identity=29.6482412060301, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364185, Length=199, Percent_Identity=29.6482412060301, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364183, Length=199, Percent_Identity=29.6482412060301, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI62471759, Length=199, Percent_Identity=29.6482412060301, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17975520, Length=199, Percent_Identity=29.6482412060301, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33226; Mature: 33226

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGILLVDKPRGWTSHDVVQWVRTRLRVQTVGHGGTLDPAAEGLLVIAVGIATRLVRHAA
CCCEEEEECCCCCCHHHHHHHHHHHHEEEECCCCCCCCHHHCCEEEEHHHHHHHHHHHHH
DADKSYVAHIVLGTGTTSDDLEGEPLAASPLSLYRPSLRDVQSALAEFLGEYEQQPPIYS
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCHHH
ALKVGGVAAYRRARAGQPVPLKRRVVRVNSLRLVQYSYPDLIVSLDCGKGFYVRSFARDL
HHHHHHHHHHHHHHCCCCCCHHHHEEEECCEEEEEEECCCEEEEEECCCCHHHHHHHHHH
GTRLGTGAYLHGLVRTRVGRFSLASAWSIDELNRVLTPSSWPLIARHPDEFVAELPVLLV
HHHCCCCHHHHHHHHHHHCCEEHHCCCCHHHHHHHCCCCCCCEEEECCHHHHHHCCEEEE
PERELRAWYHGAPVRCSASSSPGTTARAYAPDGNWIGLAHFDPQKRYWQPVLVHRDGSGW
CCHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCEEEEEEECCHHHCCCEEEEEECCCCC
RG
CC
>Mature Secondary Structure
MHGILLVDKPRGWTSHDVVQWVRTRLRVQTVGHGGTLDPAAEGLLVIAVGIATRLVRHAA
CCCEEEEECCCCCCHHHHHHHHHHHHEEEECCCCCCCCHHHCCEEEEHHHHHHHHHHHHH
DADKSYVAHIVLGTGTTSDDLEGEPLAASPLSLYRPSLRDVQSALAEFLGEYEQQPPIYS
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCHHH
ALKVGGVAAYRRARAGQPVPLKRRVVRVNSLRLVQYSYPDLIVSLDCGKGFYVRSFARDL
HHHHHHHHHHHHHHCCCCCCHHHHEEEECCEEEEEEECCCEEEEEECCCCHHHHHHHHHH
GTRLGTGAYLHGLVRTRVGRFSLASAWSIDELNRVLTPSSWPLIARHPDEFVAELPVLLV
HHHCCCCHHHHHHHHHHHCCEEHHCCCCHHHHHHHCCCCCCCEEEECCHHHHHHCCEEEE
PERELRAWYHGAPVRCSASSSPGTTARAYAPDGNWIGLAHFDPQKRYWQPVLVHRDGSGW
CCHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCEEEEEEECCHHHCCCEEEEEECCCCC
RG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA