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The map label for this gene is purK [H]

Identifier: 221632662

GI number: 221632662

Start: 637583

End: 638692

Strand: Reverse

Name: purK [H]

Synonym: trd_0645

Alternate gene names: 221632662

Gene position: 638692-637583 (Counterclockwise)

Preceding gene: 221632663

Following gene: 221632660

Centisome position: 31.89

GC content: 62.07

Gene sequence:

>1110_bases
ATGCGCATCGGCATTCTCGGCGGAGGGCAACTCGGGCGAATGCTGGCGCTCGCCGGTTATCCGCTCGGTCTGCACTTTCG
CGGTCTCGATCCAGCTCCCGAGGCCCCGCTCGGTCACGTCGCCGAACTGCTCGTGGCCTCCTACGACGACCGCCTTGCCC
TCGAGCAGTTTGCAACCGGGTTGGACTACGTCACGTATGAATTCGAGAACGTCCCGGGCACGACGGCCGAATTCCTGGCG
ACACGGCTGCCGGTTCTGCCGCCACCAGTGGCCCTGTTCCGTGCTCAAGACCGGCTGGAAGAAAAGCAACTGTTCGAATC
GTGTGGTATTCCCGTCCCTCGTTACGCGCCGATCTCGTCGCCCGAGGACTTCGGCCCGGCCATCGAAACTGTACCGTTTC
CAGCTGTTCTCAAGACGCGACGCTTCGGTTACGACGGGCATGGGCAAAAACGCGTGCGCCAGCCTGCAGAACTCCCGCGA
GCCTGGGAAGAGCTGGGTCGTGTCTCCTGCCTGCTCGAGGAGTTCGTACCGTTCGACTACGAAGTCTCGCTCCTTGGGGC
GCGTTCACGCACCGGTGAAATCGCGTTCTACCCACTCGTCGAAAATCGCCATACCGAAGGCATTCTCGCGCTCTCACGCG
CACCGGCCCCACGTGCGAACCGCACCCTACAAGAGCAAGCCGAACGCTCAGTGGCAGCTCTCCTTGAAGAACTGTCTTAC
GTCGGCATCCTGGCCGTCGAGTTCTTCGTCGTCGGCGAGACGCTCCTGGCCAACGAAATGGCACCTCGCGTGCATAACAG
TGGCCACTGGACGATCGAAGGAGCGGAAACGAGTCAGTTCGAGCAGCACCTGCGCGCTATCCTGGGATGGCCCCTCGGCT
CGACGGCGATGCGCGGCTACGCGGCGATGATCAATCTGCTCGGTTCCCTACCACCGATCGAGTCGCTCCTCGCGATTCCC
AATGTGCACGTGCACCTCTACGGGAAAACCCCGCGACCGCGTCGAAAACTGGGTCATGTCACGATCCGGGCCGAGAGCCC
CGAAGAACGGGATGAACTGGTTCGCCTCGTCCTTTCGATCGTTGAATTCCCTCAACCCGTCACGATCTAG

Upstream 100 bases:

>100_bases
ACCCCGAGATCCGCGAGCGTCTTCGCCGTTTCCGAGACGAACAAACGCGCGCAGTTCTGGAGAACCCTGACCCGCGACAG
GCACCGTGAAGGAGCGACCG

Downstream 100 bases:

>100_bases
CAAAACAGGTGGGGCTGAGTGTAGTCTCAGCCCCACCACTCGTTTCACGATCTCATTGCCAGGCAGGTGTCCGCAAGACA
GCACCCTGCGAGGCAGAGCT

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MRIGILGGGQLGRMLALAGYPLGLHFRGLDPAPEAPLGHVAELLVASYDDRLALEQFATGLDYVTYEFENVPGTTAEFLA
TRLPVLPPPVALFRAQDRLEEKQLFESCGIPVPRYAPISSPEDFGPAIETVPFPAVLKTRRFGYDGHGQKRVRQPAELPR
AWEELGRVSCLLEEFVPFDYEVSLLGARSRTGEIAFYPLVENRHTEGILALSRAPAPRANRTLQEQAERSVAALLEELSY
VGILAVEFFVVGETLLANEMAPRVHNSGHWTIEGAETSQFEQHLRAILGWPLGSTAMRGYAAMINLLGSLPPIESLLAIP
NVHVHLYGKTPRPRRKLGHVTIRAESPEERDELVRLVLSIVEFPQPVTI

Sequences:

>Translated_369_residues
MRIGILGGGQLGRMLALAGYPLGLHFRGLDPAPEAPLGHVAELLVASYDDRLALEQFATGLDYVTYEFENVPGTTAEFLA
TRLPVLPPPVALFRAQDRLEEKQLFESCGIPVPRYAPISSPEDFGPAIETVPFPAVLKTRRFGYDGHGQKRVRQPAELPR
AWEELGRVSCLLEEFVPFDYEVSLLGARSRTGEIAFYPLVENRHTEGILALSRAPAPRANRTLQEQAERSVAALLEELSY
VGILAVEFFVVGETLLANEMAPRVHNSGHWTIEGAETSQFEQHLRAILGWPLGSTAMRGYAAMINLLGSLPPIESLLAIP
NVHVHLYGKTPRPRRKLGHVTIRAESPEERDELVRLVLSIVEFPQPVTI
>Mature_369_residues
MRIGILGGGQLGRMLALAGYPLGLHFRGLDPAPEAPLGHVAELLVASYDDRLALEQFATGLDYVTYEFENVPGTTAEFLA
TRLPVLPPPVALFRAQDRLEEKQLFESCGIPVPRYAPISSPEDFGPAIETVPFPAVLKTRRFGYDGHGQKRVRQPAELPR
AWEELGRVSCLLEEFVPFDYEVSLLGARSRTGEIAFYPLVENRHTEGILALSRAPAPRANRTLQEQAERSVAALLEELSY
VGILAVEFFVVGETLLANEMAPRVHNSGHWTIEGAETSQFEQHLRAILGWPLGSTAMRGYAAMINLLGSLPPIESLLAIP
NVHVHLYGKTPRPRRKLGHVTIRAESPEERDELVRLVLSIVEFPQPVTI

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=345, Percent_Identity=39.1304347826087, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1788155, Length=368, Percent_Identity=27.7173913043478, Blast_Score=93, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324702, Length=388, Percent_Identity=34.020618556701, Blast_Score=192, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 40788; Mature: 40788

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGILGGGQLGRMLALAGYPLGLHFRGLDPAPEAPLGHVAELLVASYDDRLALEQFATG
CEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCC
LDYVTYEFENVPGTTAEFLATRLPVLPPPVALFRAQDRLEEKQLFESCGIPVPRYAPISS
CCEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PEDFGPAIETVPFPAVLKTRRFGYDGHGQKRVRQPAELPRAWEELGRVSCLLEEFVPFDY
CHHCCCCHHCCCCHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCE
EVSLLGARSRTGEIAFYPLVENRHTEGILALSRAPAPRANRTLQEQAERSVAALLEELSY
EEEEEECCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VGILAVEFFVVGETLLANEMAPRVHNSGHWTIEGAETSQFEQHLRAILGWPLGSTAMRGY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHH
AAMINLLGSLPPIESLLAIPNVHVHLYGKTPRPRRKLGHVTIRAESPEERDELVRLVLSI
HHHHHHHHCCCCHHHHHHCCCEEEEEEECCCCCHHHHCCEEEEECCCHHHHHHHHHHHHH
VEFPQPVTI
HHCCCCCCC
>Mature Secondary Structure
MRIGILGGGQLGRMLALAGYPLGLHFRGLDPAPEAPLGHVAELLVASYDDRLALEQFATG
CEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCC
LDYVTYEFENVPGTTAEFLATRLPVLPPPVALFRAQDRLEEKQLFESCGIPVPRYAPISS
CCEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PEDFGPAIETVPFPAVLKTRRFGYDGHGQKRVRQPAELPRAWEELGRVSCLLEEFVPFDY
CHHCCCCHHCCCCHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCE
EVSLLGARSRTGEIAFYPLVENRHTEGILALSRAPAPRANRTLQEQAERSVAALLEELSY
EEEEEECCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VGILAVEFFVVGETLLANEMAPRVHNSGHWTIEGAETSQFEQHLRAILGWPLGSTAMRGY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHH
AAMINLLGSLPPIESLLAIPNVHVHLYGKTPRPRRKLGHVTIRAESPEERDELVRLVLSI
HHHHHHHHCCCCHHHHHHCCCEEEEEEECCCCCHHHHCCEEEEECCCHHHHHHHHHHHHH
VEFPQPVTI
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8816818; 10984043 [H]