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The map label for this gene is pfkA2 [H]

Identifier: 221632629

GI number: 221632629

Start: 601154

End: 602281

Strand: Reverse

Name: pfkA2 [H]

Synonym: trd_0608

Alternate gene names: 221632629

Gene position: 602281-601154 (Counterclockwise)

Preceding gene: 221632631

Following gene: 221632628

Centisome position: 30.07

GC content: 64.36

Gene sequence:

>1128_bases
ATGGCAGAGAGATCGCTCCGCCGGATCGGCGTTCTCACCGGCGGGGGCGATGCCCCGGGACTCAATGCCGCCTTGCGGGC
CGTCGTTAAGACAGCCATCTTGCGCTACGGTTGGACAGTGATCGGCTTTGAGGACGGGTACGAGGGCGCGATCCTCGGCC
GCGCTCGGCAACTCGGGCTCGACGATGTCCGCGGTTTGCTCGTACGAGGTGGAACCATCCTCGGCGCTTCCAACCGTGCC
AACCCGTTCTTCTTCGCGCATCCTGAACAAGGCGACACCGAGCCGCGTGATCATAGTCGGACCCTGCTCGCCACGCTCGA
GCGCATCGGCATCGACGCCTTGATCGTCATCGGCGGCGAAGGGAGCCTGTCCATCGCGCATCGCCTCAACGAGATGGGAC
TTCGTGTCATCGGCATCCCCAAAACGATCGACAATGACGTCCGGGGCACGCTCCAGACGATCGGCTTCGATACCGCTGTC
ACCACCGCCACCGAGGCGATCGATCGCCTGCATACGACCGCCGAAAGTCACCATCGGGTCATGCTGGTGGAACTCATGGG
CCGGGACGCCGGGTGGATCGCGCTGTCCGCTGGAGTCGCTGGTGGAGCAGATGTGATCCTGATCCCGGAAATCCCGTTCG
TCCTCGACCGCGTCGTCGAGGTGATACGAGGACGACGGCAACGCAGGCGTTCCTTCACCATCGTCGCCGTCGCGGAAGGT
GCTCGGCCACTCGATGGCGAAGCAGTCTTCGAGCGCACTGGCCCGCTCCCCTATCAGCGAAAGTACGGTGGCATTTCGCT
CGTTCTCCAAAAACAGTTGAGTCGCATCCTCCCGACCGAAGTTCGCGCAGTCGTCCTCGGTCATCTCCAGCGCGGAGGTA
CGCCGACACCGACCGACCGTGCGTTGGCGACTGCACTCGGCTCGGCTGCAGTTGACGCACTCGCCGACGATGACGATGGC
GTCATGGTCGCCATCCGCGCGCACCCTGCCGGGCAAGCGACCTGGGGCATCGTACGGGTTCCATTGGCCGAGGTCGCACG
CGGCCCACGGCTGGTCCCTCTCGATCATCCCCTCGTTCGCGCAGCGCGTGGAATCGGAATCAGTTTCGCGCAACAGGACG
AGGAGTGA

Upstream 100 bases:

>100_bases
CGTAGAATGCGCAGGGGCCACGTCGAGCAGCGAAAACCCACCCGTCTCGACGTGAGTATACCCATGTTGCCGAATGCCAC
GATCGAGGAGGTTTACCACG

Downstream 100 bases:

>100_bases
ACGCGTGAGCGACAGGCCTCTACCGACCGGGAAGCTTCCTCCCCAACTCCTGGCTGAATTTCTCGGGAATCTTTCTCCGG
ATCCGAGCGTCCTGCTCGGT

Product: 6-phosphofructokinase 1

Products: NA

Alternate protein names: Phosphofructokinase 2; Phosphohexokinase 2 [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MAERSLRRIGVLTGGGDAPGLNAALRAVVKTAILRYGWTVIGFEDGYEGAILGRARQLGLDDVRGLLVRGGTILGASNRA
NPFFFAHPEQGDTEPRDHSRTLLATLERIGIDALIVIGGEGSLSIAHRLNEMGLRVIGIPKTIDNDVRGTLQTIGFDTAV
TTATEAIDRLHTTAESHHRVMLVELMGRDAGWIALSAGVAGGADVILIPEIPFVLDRVVEVIRGRRQRRRSFTIVAVAEG
ARPLDGEAVFERTGPLPYQRKYGGISLVLQKQLSRILPTEVRAVVLGHLQRGGTPTPTDRALATALGSAAVDALADDDDG
VMVAIRAHPAGQATWGIVRVPLAEVARGPRLVPLDHPLVRAARGIGISFAQQDEE

Sequences:

>Translated_375_residues
MAERSLRRIGVLTGGGDAPGLNAALRAVVKTAILRYGWTVIGFEDGYEGAILGRARQLGLDDVRGLLVRGGTILGASNRA
NPFFFAHPEQGDTEPRDHSRTLLATLERIGIDALIVIGGEGSLSIAHRLNEMGLRVIGIPKTIDNDVRGTLQTIGFDTAV
TTATEAIDRLHTTAESHHRVMLVELMGRDAGWIALSAGVAGGADVILIPEIPFVLDRVVEVIRGRRQRRRSFTIVAVAEG
ARPLDGEAVFERTGPLPYQRKYGGISLVLQKQLSRILPTEVRAVVLGHLQRGGTPTPTDRALATALGSAAVDALADDDDG
VMVAIRAHPAGQATWGIVRVPLAEVARGPRLVPLDHPLVRAARGIGISFAQQDEE
>Mature_374_residues
AERSLRRIGVLTGGGDAPGLNAALRAVVKTAILRYGWTVIGFEDGYEGAILGRARQLGLDDVRGLLVRGGTILGASNRAN
PFFFAHPEQGDTEPRDHSRTLLATLERIGIDALIVIGGEGSLSIAHRLNEMGLRVIGIPKTIDNDVRGTLQTIGFDTAVT
TATEAIDRLHTTAESHHRVMLVELMGRDAGWIALSAGVAGGADVILIPEIPFVLDRVVEVIRGRRQRRRSFTIVAVAEGA
RPLDGEAVFERTGPLPYQRKYGGISLVLQKQLSRILPTEVRAVVLGHLQRGGTPTPTDRALATALGSAAVDALADDDDGV
MVAIRAHPAGQATWGIVRVPLAEVARGPRLVPLDHPLVRAARGIGISFAQQDEE

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=381, Percent_Identity=34.3832020997375, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI11321601, Length=377, Percent_Identity=33.42175066313, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI266453619, Length=369, Percent_Identity=32.2493224932249, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI266453768, Length=369, Percent_Identity=32.2493224932249, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI266453748, Length=369, Percent_Identity=32.2493224932249, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI4505749, Length=369, Percent_Identity=32.2493224932249, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1790350, Length=309, Percent_Identity=42.3948220064725, Blast_Score=215, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI25147584, Length=377, Percent_Identity=30.2387267904509, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17558788, Length=344, Percent_Identity=31.3953488372093, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI72003601, Length=322, Percent_Identity=28.8819875776398, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321679, Length=343, Percent_Identity=36.4431486880466, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6323861, Length=379, Percent_Identity=34.0369393139842, Blast_Score=159, Evalue=7e-40,
Organism=Drosophila melanogaster, GI17647809, Length=365, Percent_Identity=33.1506849315069, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24652337, Length=365, Percent_Identity=33.1506849315069, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI28573326, Length=365, Percent_Identity=33.1506849315069, Blast_Score=154, Evalue=8e-38,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 40026; Mature: 39894

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERSLRRIGVLTGGGDAPGLNAALRAVVKTAILRYGWTVIGFEDGYEGAILGRARQLGL
CCCCCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEHHHHHCCH
DDVRGLLVRGGTILGASNRANPFFFAHPEQGDTEPRDHSRTLLATLERIGIDALIVIGGE
HHHHHHHHCCCEEEECCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCC
GSLSIAHRLNEMGLRVIGIPKTIDNDVRGTLQTIGFDTAVTTATEAIDRLHTTAESHHRV
CCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEE
MLVELMGRDAGWIALSAGVAGGADVILIPEIPFVLDRVVEVIRGRRQRRRSFTIVAVAEG
EEEEECCCCCCEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEECC
ARPLDGEAVFERTGPLPYQRKYGGISLVLQKQLSRILPTEVRAVVLGHLQRGGTPTPTDR
CCCCCCCHHHHHCCCCCCHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHH
ALATALGSAAVDALADDDDGVMVAIRAHPAGQATWGIVRVPLAEVARGPRLVPLDHPLVR
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEECCHHHHCCCCEECCCCCHHHH
AARGIGISFAQQDEE
HHHCCCCEECCCCCC
>Mature Secondary Structure 
AERSLRRIGVLTGGGDAPGLNAALRAVVKTAILRYGWTVIGFEDGYEGAILGRARQLGL
CCCCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEHHHHHCCH
DDVRGLLVRGGTILGASNRANPFFFAHPEQGDTEPRDHSRTLLATLERIGIDALIVIGGE
HHHHHHHHCCCEEEECCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCC
GSLSIAHRLNEMGLRVIGIPKTIDNDVRGTLQTIGFDTAVTTATEAIDRLHTTAESHHRV
CCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEE
MLVELMGRDAGWIALSAGVAGGADVILIPEIPFVLDRVVEVIRGRRQRRRSFTIVAVAEG
EEEEECCCCCCEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEECC
ARPLDGEAVFERTGPLPYQRKYGGISLVLQKQLSRILPTEVRAVVLGHLQRGGTPTPTDR
CCCCCCCHHHHHCCCCCCHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHH
ALATALGSAAVDALADDDDGVMVAIRAHPAGQATWGIVRVPLAEVARGPRLVPLDHPLVR
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEECCHHHHCCCCEECCCCCHHHH
AARGIGISFAQQDEE
HHHCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]