BacMap

Definition	Thermomicrobium roseum DSM 5159 chromosome, complete genome.
Accession	NC_011959
Length	2,003,006

Click here to switch to the map view.

The map label for this gene is nodG [H]

Identifier: 221632327

GI number: 221632327

Start: 301208

End: 302023

Strand: Reverse

Name: nodG [H]

Synonym: trd_0297

Alternate gene names: 221632327

Gene position: 302023-301208 (Counterclockwise)

Preceding gene: 221632328

Following gene: 221632326

Centisome position: 15.08

GC content: 63.24

Gene sequence:

>816_bases
ATGGGACGACTCACAGGACTCGTCGCCCTCGTGACCGGAGCTGGGGGCGGAGAAATGGGAGGTGGCGGCGCAGGGATCGC
ACGCGTTCTCGCTCGGGAGGGAGCGATCGTCGCCGTCAATGATCTTCATGAGGACTACGCGCGAGCGACCGTGGATCAGA
TCACCCGGAACGGTGGACAATCCTTCGCGCTCGCTGGCGACGTTTCCGATCCACGGCAAGCGAACGAGCTGGTCTCGTCG
GTCATCGCACGCTGTGGACGACTGGACATCCTCGTCAATGACGCGGCGCGCTTGGGGCGGACTCCCGCCGTCGAGCGGAT
GTCGGACGAGGAGTGGCACAGCTACATCGCGGTCAACCTCTCGGGACCCTTCTACATGAGCCGAGCAGCCATCCCCCACA
TGATGCAGCGCGAATATGGCCGAATCATCAATATCGCTTCGCTCGCTGCCCTCCGGGCGAGTCTCAACGGGGGAGCAGGG
TATACCTCGTCCAAGCGAGGATTGCTCGGTCTGACCCGGCAGCTCGCTGCCGAGTTCTATACGTACAACATCACCGTCAA
CGCGGTGCTGCCTGCTGGGATCTTGAGCGGTCGCTTGCGCGCTGAGATGGGAGAACACGCCGAAGCGCTCGTCGCAACGC
GCCGTTGGGCGTTACCAGACGATATCGGACAGATCTGCGCCTACCTGGCGAGTCCGGAAGCACACCTCATCTCGGCGAGC
GTCATCGTCGTTCCGGGCGTCAACGCCACCGGCCTGGGCGACTTCGCTCAGTATCGTGCGATGGCTCGCCAGATCGGCAA
GGATATCGGTGAGTAG

Upstream 100 bases:

>100_bases
TCACCGGTGCGGCGATTCCGATCGATGGTGCGGTTTCCGTCGTACCAGGTGGCCGTACCGATCCAGCGTCTGCCTTCGCG
TGAAGAGAGGAGCAGGTGCG

Downstream 100 bases:

>100_bases
CTCCCGGGCCACGGGGGATGCTTTTCCTCCTTGGCCGTACTGTCCTTCGAGACAGTCAGGCGCACAGGCAACCTCACCAT
TGGTCACGAAGGCAGCAGCC

Product: short chain dehydrogenase/reductase family oxidoreductase

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MGRLTGLVALVTGAGGGEMGGGGAGIARVLAREGAIVAVNDLHEDYARATVDQITRNGGQSFALAGDVSDPRQANELVSS
VIARCGRLDILVNDAARLGRTPAVERMSDEEWHSYIAVNLSGPFYMSRAAIPHMMQREYGRIINIASLAALRASLNGGAG
YTSSKRGLLGLTRQLAAEFYTYNITVNAVLPAGILSGRLRAEMGEHAEALVATRRWALPDDIGQICAYLASPEAHLISAS
VIVVPGVNATGLGDFAQYRAMARQIGKDIGE

Sequences:

>Translated_271_residues
MGRLTGLVALVTGAGGGEMGGGGAGIARVLAREGAIVAVNDLHEDYARATVDQITRNGGQSFALAGDVSDPRQANELVSS
VIARCGRLDILVNDAARLGRTPAVERMSDEEWHSYIAVNLSGPFYMSRAAIPHMMQREYGRIINIASLAALRASLNGGAG
YTSSKRGLLGLTRQLAAEFYTYNITVNAVLPAGILSGRLRAEMGEHAEALVATRRWALPDDIGQICAYLASPEAHLISAS
VIVVPGVNATGLGDFAQYRAMARQIGKDIGE
>Mature_270_residues
GRLTGLVALVTGAGGGEMGGGGAGIARVLAREGAIVAVNDLHEDYARATVDQITRNGGQSFALAGDVSDPRQANELVSSV
IARCGRLDILVNDAARLGRTPAVERMSDEEWHSYIAVNLSGPFYMSRAAIPHMMQREYGRIINIASLAALRASLNGGAGY
TSSKRGLLGLTRQLAAEFYTYNITVNAVLPAGILSGRLRAEMGEHAEALVATRRWALPDDIGQICAYLASPEAHLISASV
IVVPGVNATGLGDFAQYRAMARQIGKDIGE

Specific function: Proposed to modify Nod factor fatty acyl chain [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4504505, Length=198, Percent_Identity=37.3737373737374, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI32483357, Length=252, Percent_Identity=31.7460317460317, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI15277342, Length=255, Percent_Identity=31.3725490196078, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI19923817, Length=259, Percent_Identity=25.8687258687259, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI66933014, Length=257, Percent_Identity=27.6264591439689, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI5031737, Length=261, Percent_Identity=31.0344827586207, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI40254992, Length=226, Percent_Identity=28.3185840707965, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI33667109, Length=197, Percent_Identity=34.010152284264, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI126723191, Length=190, Percent_Identity=32.6315789473684, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI126723750, Length=255, Percent_Identity=28.6274509803922, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI59889578, Length=235, Percent_Identity=28.0851063829787, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI31542939, Length=219, Percent_Identity=28.7671232876712, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI10190704, Length=272, Percent_Identity=30.5147058823529, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI87082100, Length=264, Percent_Identity=31.8181818181818, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1789208, Length=250, Percent_Identity=34, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787335, Length=247, Percent_Identity=36.0323886639676, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1787905, Length=248, Percent_Identity=33.0645161290323, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790717, Length=248, Percent_Identity=27.8225806451613, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788459, Length=245, Percent_Identity=28.1632653061224, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87082160, Length=248, Percent_Identity=27.0161290322581, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI2367175, Length=250, Percent_Identity=29.6, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789378, Length=248, Percent_Identity=29.4354838709677, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787820, Length=246, Percent_Identity=26.8292682926829, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI2367365, Length=248, Percent_Identity=29.0322580645161, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789057, Length=250, Percent_Identity=28, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786812, Length=193, Percent_Identity=29.0155440414508, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17568967, Length=198, Percent_Identity=34.3434343434343, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17561402, Length=257, Percent_Identity=31.1284046692607, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17562910, Length=264, Percent_Identity=28.7878787878788, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17555706, Length=264, Percent_Identity=29.9242424242424, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560676, Length=248, Percent_Identity=27.8225806451613, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17562906, Length=262, Percent_Identity=27.8625954198473, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17562908, Length=286, Percent_Identity=27.972027972028, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17560332, Length=291, Percent_Identity=28.1786941580756, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17564282, Length=192, Percent_Identity=28.125, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=254, Percent_Identity=29.9212598425197, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560150, Length=261, Percent_Identity=24.904214559387, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193203117, Length=208, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193203115, Length=190, Percent_Identity=27.8947368421053, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=248, Percent_Identity=25.8064516129032, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI193204405, Length=263, Percent_Identity=26.2357414448669, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI72000259, Length=260, Percent_Identity=25, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=271, Percent_Identity=26.5682656826568, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17536025, Length=269, Percent_Identity=27.8810408921933, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=240, Percent_Identity=30, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562990, Length=268, Percent_Identity=26.1194029850746, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17508895, Length=169, Percent_Identity=29.585798816568, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17536651, Length=269, Percent_Identity=26.3940520446097, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508651, Length=236, Percent_Identity=25.4237288135593, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=193, Percent_Identity=37.3056994818653, Blast_Score=112, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24639444, Length=252, Percent_Identity=30.1587301587302, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI18859837, Length=198, Percent_Identity=34.8484848484849, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI23397609, Length=257, Percent_Identity=27.6264591439689, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355319, Length=252, Percent_Identity=26.1904761904762, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21357041, Length=246, Percent_Identity=27.6422764227642, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571526, Length=256, Percent_Identity=28.125, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24644339, Length=254, Percent_Identity=27.9527559055118, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21357673, Length=200, Percent_Identity=28.5, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21355631, Length=179, Percent_Identity=31.2849162011173, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24640492, Length=200, Percent_Identity=27, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011283
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 28535; Mature: 28404

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLTGLVALVTGAGGGEMGGGGAGIARVLAREGAIVAVNDLHEDYARATVDQITRNGGQ
CCCHHEEEHEEECCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCC
SFALAGDVSDPRQANELVSSVIARCGRLDILVNDAARLGRTPAVERMSDEEWHSYIAVNL
EEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHCCCCCHHEEEEEEE
SGPFYMSRAAIPHMMQREYGRIINIASLAALRASLNGGAGYTSSKRGLLGLTRQLAAEFY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHE
TYNITVNAVLPAGILSGRLRAEMGEHAEALVATRRWALPDDIGQICAYLASPEAHLISAS
EEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHEEEEE
VIVVPGVNATGLGDFAQYRAMARQIGKDIGE
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GRLTGLVALVTGAGGGEMGGGGAGIARVLAREGAIVAVNDLHEDYARATVDQITRNGGQ
CCHHEEEHEEECCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCC
SFALAGDVSDPRQANELVSSVIARCGRLDILVNDAARLGRTPAVERMSDEEWHSYIAVNL
EEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHCCCCCHHEEEEEEE
SGPFYMSRAAIPHMMQREYGRIINIASLAALRASLNGGAGYTSSKRGLLGLTRQLAAEFY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHE
TYNITVNAVLPAGILSGRLRAEMGEHAEALVATRRWALPDDIGQICAYLASPEAHLISAS
EEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHEEEEE
VIVVPGVNATGLGDFAQYRAMARQIGKDIGE
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1538694; 2243795 [H]