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The map label for this gene is tilS/hprT [H]

Identifier: 221632326

GI number: 221632326

Start: 298788

End: 300875

Strand: Reverse

Name: tilS/hprT [H]

Synonym: trd_0295

Alternate gene names: 221632326

Gene position: 300875-298788 (Counterclockwise)

Preceding gene: 221632327

Following gene: 221632325

Centisome position: 15.02

GC content: 63.51

Gene sequence:

>2088_bases
GTGGTTCGAATCCGCCCACCGGCACCGAACGGAGCGGCGCAAGTTGCGCCGCTTTCGCTTTTGGGCGAGAATCACTCGAG
CGACTCGCCGATATGGGGGCGCACGCTCGTGAGACGCTCCAATTCGGCCATCAGTGCAGTCCTACGCGTCGTCCGTACGG
CGTTGGAAGAAGCAGGCCTGTCAGGGCACGATCGGATCATCGTGGGATTCTCGGGTGGACCGGATTCCCTCGGCCTGCTC
TGGAGCCTCCAGCACCTGGCCCACCGAGGGCTCGCTCCGACACCGATCCCTGTCCATATCGATCATGGCCTGCAATCGAG
CTCCAGCACAATGGCCGAGCGCGCTATCGCCTTGGGCAGCGAACTCGGCCTGGTGGTACGAGTCGTGCAAGTCAATGTTT
CTCTCTGGCCTGAGTACAAGGAGGGCGGGTTGGAGGCAGGAGCGCGAGCCGCTCGCTACGCCGCACTCGCTCAGGTCGCA
CGGGCGGAGGGAACATCTTGGATCACGGTCGGCCATACGCGTGACGATCAAGCAGAAACGGTACTGCTGCGCCTGCTCCG
TGGTGCTGGGCTGGACGGCCTCGCGGCGATGCGACCGGTCACCGAACTCCGCATTCCACTCGACCCACTGCACCGCGAGT
TCGCGTCCATCCGCTTGATCCGTCCGCTCCTCAGGATTCGACGATCAGTCGTACGAGCAGCTGTGGAATTACTCGGCCTC
CAACCGCTGGAAGACCCCACGAATCTGCTCCTCTCGTACGAGCGCAATGCGATTCGCCGACGAGTCCTTCCGATACTGGA
GCAACTCCGCCCCGGTACGACGGACGCGCTCGCGCAGGTCGCAGAGCAGTTGCGCGAAGATGCGGAATTTCTCCAGCGGG
TAGCCCGGGAAACCTCGGCACAGGTTTTCCGACCTGAGGCCGACTTCTTGATCCTCGATCGGGCGGCGTTTCGCGCGCTG
GACCCGGCTCTGCAACGGCGGATTCTGCAAACAGCGCTGCGCGAGCACTGGGATCCGACCTGGAGCTTGAGTCGTGAGCG
GCTGGTAGCCCTCCGAAAAGCACTCGTCGAGGGCCAGACGGGGCGAGCGATCGAACTCGGCCATGGTCTCGTCGCTGTGA
TCAGCTACACTGAAGCCGTTCTCGGTCCGGGCAACCGGATCGAGCAATACCTGCGGAACCGCTCCGGGCGTCCGCTCATC
CAGCCTGGTAGTGTCCTGCCGCTCCGCAGCGGCACGACTCTCGCGCTGGAGAACGAGTGGGAGCTCGCGATCCTGCGCGC
GCCCGCCGAGGGATGGGTCGTGCGCACCCGGCGTCCTGGTGACCGCCTGGTCCGTCCGGGCCAGCCGGTTCCGGTTCGCC
TTCAGGACTGGTTGGTCAACGAGAAAGTTCCGAGCTATCTTCGCGACTGGCTACCGCTGGTAGCCAGTGACGGGATCGTG
CACTGGATTCCTGGCCTGCAAGGCGAGCGCTACCGCTCTCCAGACGGTACCGTCGTGCTCGAACTGGTTCGCAAGGGAGG
AGTACGAGGCGTGAGCGAAACAGCACAGGCTCCCAGCGGAGAACTCGAGCGGGTCCTCATCGATGAAGCGACGCTGCAGC
GGCGAGTGGCCGAACTCGGTGAGGAAATCGCCCGCGCTTACCAGGGAAAGCGCCCTGTGCTCATCGGCGTTTTGACGGGA
GCATTCGTCTTCATGGCTGATTTGGTTCGGCACCTTCCCATCGACCTCGACATCGACTTCATGGCTGTTTCCAGCTACGG
ACAGGCTACCGTCACATCCGGTGTCGTCCGCATCATCAAGGATCTCGATCGCCCGATCGAGGGACGAGACGTTCTCCTCG
TCGAAGACATCATCGATAGCGGTCTGACCCTGCAATATCTTCTGGACGTTCTCCAGCGCCGCAACCCGCGCAGCCTGCGC
GTCGTTGTCCTCCTGCGCAAGCAGAAGCCAGGTGCCGTCAGTGTTCGAGCGGACTGGGTCGGCTTCGACATTCCGGACGA
GTTCGTGGTCGGCTACGGCCTCGATGCAGCGGGGAGGTTCCGCAATCTCCCCTTCATCGCCGTCTATCGGAACGCAGCGC
AAGGGTGA

Upstream 100 bases:

>100_bases
GGAAACTTGACGGCTGCCGAGCGGGAATGCTAGACTTCGACGCGGGAAGTGGGCCGGTGTAGCTCAACGGTAGAGCAGGG
GACTCATAAGCCCTTGGTTG

Downstream 100 bases:

>100_bases
TTTCCGGTCGCCTACCCGATTACGACTGGCAGCCGGTCAGCTGCGCTCCGAGGATGCTCGTTCCTGCAAAGGAGCTGAGC
GATTGAACCGAGCCCATCCG

Product: putative bifunctional protein tilS/hprT

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 695; Mature: 695

Protein sequence:

>695_residues
MVRIRPPAPNGAAQVAPLSLLGENHSSDSPIWGRTLVRRSNSAISAVLRVVRTALEEAGLSGHDRIIVGFSGGPDSLGLL
WSLQHLAHRGLAPTPIPVHIDHGLQSSSSTMAERAIALGSELGLVVRVVQVNVSLWPEYKEGGLEAGARAARYAALAQVA
RAEGTSWITVGHTRDDQAETVLLRLLRGAGLDGLAAMRPVTELRIPLDPLHREFASIRLIRPLLRIRRSVVRAAVELLGL
QPLEDPTNLLLSYERNAIRRRVLPILEQLRPGTTDALAQVAEQLREDAEFLQRVARETSAQVFRPEADFLILDRAAFRAL
DPALQRRILQTALREHWDPTWSLSRERLVALRKALVEGQTGRAIELGHGLVAVISYTEAVLGPGNRIEQYLRNRSGRPLI
QPGSVLPLRSGTTLALENEWELAILRAPAEGWVVRTRRPGDRLVRPGQPVPVRLQDWLVNEKVPSYLRDWLPLVASDGIV
HWIPGLQGERYRSPDGTVVLELVRKGGVRGVSETAQAPSGELERVLIDEATLQRRVAELGEEIARAYQGKRPVLIGVLTG
AFVFMADLVRHLPIDLDIDFMAVSSYGQATVTSGVVRIIKDLDRPIEGRDVLLVEDIIDSGLTLQYLLDVLQRRNPRSLR
VVVLLRKQKPGAVSVRADWVGFDIPDEFVVGYGLDAAGRFRNLPFIAVYRNAAQG

Sequences:

>Translated_695_residues
MVRIRPPAPNGAAQVAPLSLLGENHSSDSPIWGRTLVRRSNSAISAVLRVVRTALEEAGLSGHDRIIVGFSGGPDSLGLL
WSLQHLAHRGLAPTPIPVHIDHGLQSSSSTMAERAIALGSELGLVVRVVQVNVSLWPEYKEGGLEAGARAARYAALAQVA
RAEGTSWITVGHTRDDQAETVLLRLLRGAGLDGLAAMRPVTELRIPLDPLHREFASIRLIRPLLRIRRSVVRAAVELLGL
QPLEDPTNLLLSYERNAIRRRVLPILEQLRPGTTDALAQVAEQLREDAEFLQRVARETSAQVFRPEADFLILDRAAFRAL
DPALQRRILQTALREHWDPTWSLSRERLVALRKALVEGQTGRAIELGHGLVAVISYTEAVLGPGNRIEQYLRNRSGRPLI
QPGSVLPLRSGTTLALENEWELAILRAPAEGWVVRTRRPGDRLVRPGQPVPVRLQDWLVNEKVPSYLRDWLPLVASDGIV
HWIPGLQGERYRSPDGTVVLELVRKGGVRGVSETAQAPSGELERVLIDEATLQRRVAELGEEIARAYQGKRPVLIGVLTG
AFVFMADLVRHLPIDLDIDFMAVSSYGQATVTSGVVRIIKDLDRPIEGRDVLLVEDIIDSGLTLQYLLDVLQRRNPRSLR
VVVLLRKQKPGAVSVRADWVGFDIPDEFVVGYGLDAAGRFRNLPFIAVYRNAAQG
>Mature_695_residues
MVRIRPPAPNGAAQVAPLSLLGENHSSDSPIWGRTLVRRSNSAISAVLRVVRTALEEAGLSGHDRIIVGFSGGPDSLGLL
WSLQHLAHRGLAPTPIPVHIDHGLQSSSSTMAERAIALGSELGLVVRVVQVNVSLWPEYKEGGLEAGARAARYAALAQVA
RAEGTSWITVGHTRDDQAETVLLRLLRGAGLDGLAAMRPVTELRIPLDPLHREFASIRLIRPLLRIRRSVVRAAVELLGL
QPLEDPTNLLLSYERNAIRRRVLPILEQLRPGTTDALAQVAEQLREDAEFLQRVARETSAQVFRPEADFLILDRAAFRAL
DPALQRRILQTALREHWDPTWSLSRERLVALRKALVEGQTGRAIELGHGLVAVISYTEAVLGPGNRIEQYLRNRSGRPLI
QPGSVLPLRSGTTLALENEWELAILRAPAEGWVVRTRRPGDRLVRPGQPVPVRLQDWLVNEKVPSYLRDWLPLVASDGIV
HWIPGLQGERYRSPDGTVVLELVRKGGVRGVSETAQAPSGELERVLIDEATLQRRVAELGEEIARAYQGKRPVLIGVLTG
AFVFMADLVRHLPIDLDIDFMAVSSYGQATVTSGVVRIIKDLDRPIEGRDVLLVEDIIDSGLTLQYLLDVLQRRNPRSLR
VVVLLRKQKPGAVSVRADWVGFDIPDEFVVGYGLDAAGRFRNLPFIAVYRNAAQG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=177, Percent_Identity=36.1581920903955, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI9910262, Length=179, Percent_Identity=33.5195530726257, Blast_Score=99, Evalue=2e-20,
Organism=Escherichia coli, GI87081689, Length=165, Percent_Identity=49.6969696969697, Blast_Score=162, Evalue=9e-41,
Organism=Escherichia coli, GI1786386, Length=221, Percent_Identity=37.5565610859729, Blast_Score=95, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17510817, Length=184, Percent_Identity=36.4130434782609, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 76624; Mature: 76624

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRIRPPAPNGAAQVAPLSLLGENHSSDSPIWGRTLVRRSNSAISAVLRVVRTALEEAGL
CEEECCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
SGHDRIIVGFSGGPDSLGLLWSLQHLAHRGLAPTPIPVHIDHGLQSSSSTMAERAIALGS
CCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCC
ELGLVVRVVQVNVSLWPEYKEGGLEAGARAARYAALAQVARAEGTSWITVGHTRDDQAET
HHHHEEEEEEECCEECCCHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
VLLRLLRGAGLDGLAAMRPVTELRIPLDPLHREFASIRLIRPLLRIRRSVVRAAVELLGL
HHHHHHHCCCCCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPLEDPTNLLLSYERNAIRRRVLPILEQLRPGTTDALAQVAEQLREDAEFLQRVARETSA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVFRPEADFLILDRAAFRALDPALQRRILQTALREHWDPTWSLSRERLVALRKALVEGQT
HHCCCCCCEEEECHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
GRAIELGHGLVAVISYTEAVLGPGNRIEQYLRNRSGRPLIQPGSVLPLRSGTTLALENEW
CCEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEECCCC
ELAILRAPAEGWVVRTRRPGDRLVRPGQPVPVRLQDWLVNEKVPSYLRDWLPLVASDGIV
EEEEEECCCCCCEEEECCCCHHCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCEE
HWIPGLQGERYRSPDGTVVLELVRKGGVRGVSETAQAPSGELERVLIDEATLQRRVAELG
EECCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
EEIARAYQGKRPVLIGVLTGAFVFMADLVRHLPIDLDIDFMAVSSYGQATVTSGVVRIIK
HHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
DLDRPIEGRDVLLVEDIIDSGLTLQYLLDVLQRRNPRSLRVVVLLRKQKPGAVSVRADWV
HHCCCCCCCCEEEEHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEEEEE
GFDIPDEFVVGYGLDAAGRFRNLPFIAVYRNAAQG
CCCCCHHHHEECCCCCCCHHCCCCEEEEECCCCCC
>Mature Secondary Structure
MVRIRPPAPNGAAQVAPLSLLGENHSSDSPIWGRTLVRRSNSAISAVLRVVRTALEEAGL
CEEECCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
SGHDRIIVGFSGGPDSLGLLWSLQHLAHRGLAPTPIPVHIDHGLQSSSSTMAERAIALGS
CCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCC
ELGLVVRVVQVNVSLWPEYKEGGLEAGARAARYAALAQVARAEGTSWITVGHTRDDQAET
HHHHEEEEEEECCEECCCHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
VLLRLLRGAGLDGLAAMRPVTELRIPLDPLHREFASIRLIRPLLRIRRSVVRAAVELLGL
HHHHHHHCCCCCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPLEDPTNLLLSYERNAIRRRVLPILEQLRPGTTDALAQVAEQLREDAEFLQRVARETSA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVFRPEADFLILDRAAFRALDPALQRRILQTALREHWDPTWSLSRERLVALRKALVEGQT
HHCCCCCCEEEECHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
GRAIELGHGLVAVISYTEAVLGPGNRIEQYLRNRSGRPLIQPGSVLPLRSGTTLALENEW
CCEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEECCCC
ELAILRAPAEGWVVRTRRPGDRLVRPGQPVPVRLQDWLVNEKVPSYLRDWLPLVASDGIV
EEEEEECCCCCCEEEECCCCHHCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCEE
HWIPGLQGERYRSPDGTVVLELVRKGGVRGVSETAQAPSGELERVLIDEATLQRRVAELG
EECCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
EEIARAYQGKRPVLIGVLTGAFVFMADLVRHLPIDLDIDFMAVSSYGQATVTSGVVRIIK
HHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
DLDRPIEGRDVLLVEDIIDSGLTLQYLLDVLQRRNPRSLRVVVLLRKQKPGAVSVRADWV
HHCCCCCCCCEEEEHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEEEEE
GFDIPDEFVVGYGLDAAGRFRNLPFIAVYRNAAQG
CCCCCHHHHEECCCCCCCHHCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]