Click here to switch to the map view.

The map label for this gene is sbcD [H]

Identifier: 221632298

GI number: 221632298

Start: 271657

End: 272916

Strand: Reverse

Name: sbcD [H]

Synonym: trd_0265

Alternate gene names: 221632298

Gene position: 272916-271657 (Counterclockwise)

Preceding gene: 221632299

Following gene: 221632291

Centisome position: 13.63

GC content: 65.79

Gene sequence:

>1260_bases
ATGCTTCGCCTGATCCACTTCGCTGATCTGCATCTGGGCGTCGAGACGCACGGGCAGTTCCGTCCGGATCTCGGCCATAG
CTCCCGCATCCAGGACTTTCTCGACGCCTTCGATCAGATCATCGATGCGGCGATCGAGGAGCGGTTCGATGCCGTCCTTT
TCGCCGGCGATGCGTTCAAACACGCCGAGCCGAGCCCGACCCTTCAGCGCCGCTTTGCCGAGCGCATCCAGCGCCTCCTC
GCTGCGGGAATCCCGATCGTGCTCCTCGTCGGAAACCATGACCGGCCACGCAGCGTGGTGCGTTCGACACCGATCGATAT
CTACGCTGCACTGCGTCTACCCGGCGTCATCGTCGCCAACCGGCCGGAGCGGTTCACCATCGCGACCCCGAGCGGCCCAC
TCCAGGTTGTCGCGCTGCCCTGGATACCACCCCGTCTCCTGCTGGCCGACGATGCGCTTCGCGAGGCCGGCACTCCGGAA
TTGGAGCGTCGCTACAAGGAAGTCATCGCGTCTGCGCTGGCTCCCCTCCTCGCTGCACTCGACGCAGCGACCCCGGCGGT
TTTCCTCGGTCACCTCTCGATGGAAGGCGGACGCTTCGGGAGCGAACGCAGCGTGATCCTCGGTAACGACCCGCTCTTCG
GACTCGACGAGCTCGGGTTGAACAGCGCTCCCATCGATTACGTCGCGCTCGGCCATCTCCATTCGCATCAAGTGCTCCAC
GAGCGGCCACCCGTGGTGTATGCCGGTAGCGTCGAGCGGATCGATTTTGGAGAGGAGCGCGAGGACAAAGGCTACGTCGC
GGTCCGGATCGCCGCTGGACCATACCCGCGCGACGTCAGCTGGGAGTTTCGGCCGCTCCGTACCCGCCCGATGCGCACAT
TCCGTCTCGACATTCTGACCGACACACCGATGGACGAGATCCGCCGCGCACTCGAGCGGCACGCGACGCTGATCCCGGAG
GCGATCGTCCGCGTCACCATCACCACGACCCCAGAGCGCGCGGCAGCCATTCGTCCCCTCGAGGTGCGGCGCTGGCTCAG
CGAGTTGGGCGCCGCACACGTCGCCGGTATCACGGTACAGACCGAACGCGTCGCGCGTCCCCGCGTCGAACTCGAACGGT
CCTCCCGTCACGATCCGGTGAGCCTCTTGGAGCGCTGGGTCAACCTGCATCGTCTTTCGGACCAACTCGCCAAAGCCGTC
ATCCAGCGCGGCCGGGAAATCATCGAGCGCGTCGAGAGTCGCAGCTCGGAGCCGCGCTGA

Upstream 100 bases:

>100_bases
CGCGAGGAGATCCTCGCGTTGGTTCGCGGTTGGGGAAAGACGATGGACGACGAGCGCCATCTGCGGCTGCTCCTCGGCCA
GTGACCTGAAGGCAGAGACG

Downstream 100 bases:

>100_bases
AGCGCTCTAGAGATACGTCCGGAACCAGCGCAACTCGTGCTCGAGGTGCTCGAGCCGGTGACGTGGCCGGCCGCTTCGAG
TCAGGTTGTGACCTTCACCC

Product: putative nuclease SbcCD, D subunit subfamily

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MLRLIHFADLHLGVETHGQFRPDLGHSSRIQDFLDAFDQIIDAAIEERFDAVLFAGDAFKHAEPSPTLQRRFAERIQRLL
AAGIPIVLLVGNHDRPRSVVRSTPIDIYAALRLPGVIVANRPERFTIATPSGPLQVVALPWIPPRLLLADDALREAGTPE
LERRYKEVIASALAPLLAALDAATPAVFLGHLSMEGGRFGSERSVILGNDPLFGLDELGLNSAPIDYVALGHLHSHQVLH
ERPPVVYAGSVERIDFGEEREDKGYVAVRIAAGPYPRDVSWEFRPLRTRPMRTFRLDILTDTPMDEIRRALERHATLIPE
AIVRVTITTTPERAAAIRPLEVRRWLSELGAAHVAGITVQTERVARPRVELERSSRHDPVSLLERWVNLHRLSDQLAKAV
IQRGREIIERVESRSSEPR

Sequences:

>Translated_419_residues
MLRLIHFADLHLGVETHGQFRPDLGHSSRIQDFLDAFDQIIDAAIEERFDAVLFAGDAFKHAEPSPTLQRRFAERIQRLL
AAGIPIVLLVGNHDRPRSVVRSTPIDIYAALRLPGVIVANRPERFTIATPSGPLQVVALPWIPPRLLLADDALREAGTPE
LERRYKEVIASALAPLLAALDAATPAVFLGHLSMEGGRFGSERSVILGNDPLFGLDELGLNSAPIDYVALGHLHSHQVLH
ERPPVVYAGSVERIDFGEEREDKGYVAVRIAAGPYPRDVSWEFRPLRTRPMRTFRLDILTDTPMDEIRRALERHATLIPE
AIVRVTITTTPERAAAIRPLEVRRWLSELGAAHVAGITVQTERVARPRVELERSSRHDPVSLLERWVNLHRLSDQLAKAV
IQRGREIIERVESRSSEPR
>Mature_419_residues
MLRLIHFADLHLGVETHGQFRPDLGHSSRIQDFLDAFDQIIDAAIEERFDAVLFAGDAFKHAEPSPTLQRRFAERIQRLL
AAGIPIVLLVGNHDRPRSVVRSTPIDIYAALRLPGVIVANRPERFTIATPSGPLQVVALPWIPPRLLLADDALREAGTPE
LERRYKEVIASALAPLLAALDAATPAVFLGHLSMEGGRFGSERSVILGNDPLFGLDELGLNSAPIDYVALGHLHSHQVLH
ERPPVVYAGSVERIDFGEEREDKGYVAVRIAAGPYPRDVSWEFRPLRTRPMRTFRLDILTDTPMDEIRRALERHATLIPE
AIVRVTITTTPERAAAIRPLEVRRWLSELGAAHVAGITVQTERVARPRVELERSSRHDPVSLLERWVNLHRLSDQLAKAV
IQRGREIIERVESRSSEPR

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=343, Percent_Identity=26.530612244898, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 46928; Mature: 46928

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLIHFADLHLGVETHGQFRPDLGHSSRIQDFLDAFDQIIDAAIEERFDAVLFAGDAFK
CEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCC
HAEPSPTLQRRFAERIQRLLAAGIPIVLLVGNHDRPRSVVRSTPIDIYAALRLPGVIVAN
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCHHEEEHHHCCEEEEEC
RPERFTIATPSGPLQVVALPWIPPRLLLADDALREAGTPELERRYKEVIASALAPLLAAL
CCCEEEEECCCCCEEEEEECCCCCHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
DAATPAVFLGHLSMEGGRFGSERSVILGNDPLFGLDELGLNSAPIDYVALGHLHSHQVLH
HCCCHHHHHHHEECCCCCCCCCCEEEECCCCCCCHHHCCCCCCCCCCEEECCHHHHHHHH
ERPPVVYAGSVERIDFGEEREDKGYVAVRIAAGPYPRDVSWEFRPLRTRPMRTFRLDILT
CCCCEEEECCEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEC
DTPMDEIRRALERHATLIPEAIVRVTITTTPERAAAIRPLEVRRWLSELGAAHVAGITVQ
CCCHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHCCHHHHHHHHHHHCCHHHEEEEEE
TERVARPRVELERSSRHDPVSLLERWVNLHRLSDQLAKAVIQRGREIIERVESRSSEPR
HHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLRLIHFADLHLGVETHGQFRPDLGHSSRIQDFLDAFDQIIDAAIEERFDAVLFAGDAFK
CEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCC
HAEPSPTLQRRFAERIQRLLAAGIPIVLLVGNHDRPRSVVRSTPIDIYAALRLPGVIVAN
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCHHEEEHHHCCEEEEEC
RPERFTIATPSGPLQVVALPWIPPRLLLADDALREAGTPELERRYKEVIASALAPLLAAL
CCCEEEEECCCCCEEEEEECCCCCHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
DAATPAVFLGHLSMEGGRFGSERSVILGNDPLFGLDELGLNSAPIDYVALGHLHSHQVLH
HCCCHHHHHHHEECCCCCCCCCCEEEECCCCCCCHHHCCCCCCCCCCEEECCHHHHHHHH
ERPPVVYAGSVERIDFGEEREDKGYVAVRIAAGPYPRDVSWEFRPLRTRPMRTFRLDILT
CCCCEEEECCEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEC
DTPMDEIRRALERHATLIPEAIVRVTITTTPERAAAIRPLEVRRWLSELGAAHVAGITVQ
CCCHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHCCHHHHHHHHHHHCCHHHEEEEEE
TERVARPRVELERSSRHDPVSLLERWVNLHRLSDQLAKAVIQRGREIIERVESRSSEPR
HHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9256491 [H]