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The map label for this gene is gcvH2 [H]

Identifier: 221632191

GI number: 221632191

Start: 165375

End: 165857

Strand: Reverse

Name: gcvH2 [H]

Synonym: trd_0157

Alternate gene names: 221632191

Gene position: 165857-165375 (Counterclockwise)

Preceding gene: 221632192

Following gene: 221632190

Centisome position: 8.28

GC content: 64.18

Gene sequence:

>483_bases
ATGAGGGAGACTGCCGGCTACGCGATCGTCCATAGTTGCATCCTGCCACTCGACCTGTACTATCGAGTGGAATCGCACCA
ATGGGTACGGGTGGAAGCGGACGGATCGGTGCGGCTCGGCTACACCGATGTTGCCCAAACGGTCGCTGGTCGCATCCTTC
AGGTGACCTTCCGTCCGACCGGACGCCACTACGCGCGAGACGCCACGGTCGCAGTCGTGGAGAGTGCGAAGTGGCTCGGC
GCCTACCGGACACCGATCGCCGGCGTACTGCTCGAGACCAATACTGCCCTGCTCGAGGATCCGGAGCTGGTCAACCGTAG
TCCCTATCGTCGCGGTTGGCTGGTTCGGCTGGCCCCCGATGACCTCGCGCACGATCTCAGCGAACTCCTGAGTGGCCCAG
CGGCGGTCGCCGCCTACGATGCGTTCATGACGCGCCGCGGGCTCGATGACTGCGTTCATTGCGAGGGGTACGAACTGCCG
TGA

Upstream 100 bases:

>100_bases
ACGGCCTGACGATGGACGACCTCATCGAGGGAGTGCAGATGATCGGCGGTGCGGCGTTCAACCTGATGGCGTTGGAGGCC
GACGCAGTGGTGGCGTTCTG

Downstream 100 bases:

>100_bases
CGCGGATCAGGCTCTTCACACATCCGATCTGCTCTGGTTGCGCGGAAGCGCTCGCGCTGGTGCAGCGCCTGGCTGCCGAA
GAGCCGGAGATTCAGGTAGA

Product: glycine cleavage H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MRETAGYAIVHSCILPLDLYYRVESHQWVRVEADGSVRLGYTDVAQTVAGRILQVTFRPTGRHYARDATVAVVESAKWLG
AYRTPIAGVLLETNTALLEDPELVNRSPYRRGWLVRLAPDDLAHDLSELLSGPAAVAAYDAFMTRRGLDDCVHCEGYELP

Sequences:

>Translated_160_residues
MRETAGYAIVHSCILPLDLYYRVESHQWVRVEADGSVRLGYTDVAQTVAGRILQVTFRPTGRHYARDATVAVVESAKWLG
AYRTPIAGVLLETNTALLEDPELVNRSPYRRGWLVRLAPDDLAHDLSELLSGPAAVAAYDAFMTRRGLDDCVHCEGYELP
>Mature_160_residues
MRETAGYAIVHSCILPLDLYYRVESHQWVRVEADGSVRLGYTDVAQTVAGRILQVTFRPTGRHYARDATVAVVESAKWLG
AYRTPIAGVLLETNTALLEDPELVNRSPYRRGWLVRLAPDDLAHDLSELLSGPAAVAAYDAFMTRRGLDDCVHCEGYELP

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=36.4341085271318, Blast_Score=73, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 17802; Mature: 17802

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRETAGYAIVHSCILPLDLYYRVESHQWVRVEADGSVRLGYTDVAQTVAGRILQVTFRPT
CCCCHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCEEECHHHHHHHHHCEEEEEEECCC
GRHYARDATVAVVESAKWLGAYRTPIAGVLLETNTALLEDPELVNRSPYRRGWLVRLAPD
CCCHHCCHHEEEEHHHHHHHHHCCCCEEEEEECCCCEECCCHHHCCCCCCCCCEEEECCH
DLAHDLSELLSGPAAVAAYDAFMTRRGLDDCVHCEGYELP
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHCCCCCCCCC
>Mature Secondary Structure
MRETAGYAIVHSCILPLDLYYRVESHQWVRVEADGSVRLGYTDVAQTVAGRILQVTFRPT
CCCCHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCEEECHHHHHHHHHCEEEEEEECCC
GRHYARDATVAVVESAKWLGAYRTPIAGVLLETNTALLEDPELVNRSPYRRGWLVRLAPD
CCCHHCCHHEEEEHHHHHHHHHCCCCEEEEEECCCCEECCCHHHCCCCCCCCCEEEECCH
DLAHDLSELLSGPAAVAAYDAFMTRRGLDDCVHCEGYELP
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]