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The map label for this gene is gcvH5 [H]

Identifier: 221632178

GI number: 221632178

Start: 154714

End: 155151

Strand: Reverse

Name: gcvH5 [H]

Synonym: trd_0144

Alternate gene names: 221632178

Gene position: 155151-154714 (Counterclockwise)

Preceding gene: 221632179

Following gene: 221632177

Centisome position: 7.75

GC content: 62.1

Gene sequence:

>438_bases
ATGGCTGAGGTACACGGCTGCTCGATCCCAGAGGATCGGTATTACTGGCCGGAAAAGCATGTGTGGGCCCGCCCCGAGCC
GGATGGGACAGTGACGATCGGCATTACCGACGTCGCGCAACATCTCGCCAAGACGATCATCAGCGTGACACCACGTGGCG
TGGGGCGCAAGGTCCAGCGCGGCAAGAGTGCCGGAACCTTAGAGAGCGGGAAGTGGGTCGGCCCAGTGACCTCACCGATT
TCCGGCGAGATCGTGGCGGTCAACGACGCAGCGATCGCTGATCCCAAACTCCTCAATCAGGACCCGTACGGGGCCGGATG
GTTCGCCCGCCTGCGGCCGGATGACTGGGAAAACGAGTCGAAACTCTTGGTAACCGGTCCGGAGGCCGTCGAAGCCTACC
GGAAGGTCTTGGAACAGGAAGGGATTTCCTGCTCGTGA

Upstream 100 bases:

>100_bases
GGCTTCCTCGACGAGTACGCCTACGAGATTCGCCTGGTGTTGTTCGACCTACTCCTCCACCATATCCGCGGGGAACATGA
AACGGAAGGTGAGGCGAGAG

Downstream 100 bases:

>100_bases
GCTTTGCCGAAACGACTCGCGTCTATCGCGGCTGCGTCATCCCGCTCGACCTGCTCTACGATGTCGAGCGCGACGTCTGG
GTGCGCCGAGAGGGAGAATT

Product: GcvH

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MAEVHGCSIPEDRYYWPEKHVWARPEPDGTVTIGITDVAQHLAKTIISVTPRGVGRKVQRGKSAGTLESGKWVGPVTSPI
SGEIVAVNDAAIADPKLLNQDPYGAGWFARLRPDDWENESKLLVTGPEAVEAYRKVLEQEGISCS

Sequences:

>Translated_145_residues
MAEVHGCSIPEDRYYWPEKHVWARPEPDGTVTIGITDVAQHLAKTIISVTPRGVGRKVQRGKSAGTLESGKWVGPVTSPI
SGEIVAVNDAAIADPKLLNQDPYGAGWFARLRPDDWENESKLLVTGPEAVEAYRKVLEQEGISCS
>Mature_144_residues
AEVHGCSIPEDRYYWPEKHVWARPEPDGTVTIGITDVAQHLAKTIISVTPRGVGRKVQRGKSAGTLESGKWVGPVTSPIS
GEIVAVNDAAIADPKLLNQDPYGAGWFARLRPDDWENESKLLVTGPEAVEAYRKVLEQEGISCS

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=124, Percent_Identity=31.4516129032258, Blast_Score=66, Evalue=9e-12,
Organism=Escherichia coli, GI1789271, Length=126, Percent_Identity=40.4761904761905, Blast_Score=88, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 15793; Mature: 15662

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVHGCSIPEDRYYWPEKHVWARPEPDGTVTIGITDVAQHLAKTIISVTPRGVGRKVQR
CCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHC
GKSAGTLESGKWVGPVTSPISGEIVAVNDAAIADPKLLNQDPYGAGWFARLRPDDWENES
CCCCCCCCCCCEECCCCCCCCCCEEEECCCCCCCCHHCCCCCCCCCEEEEECCCCCCCCC
KLLVTGPEAVEAYRKVLEQEGISCS
EEEEECHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AEVHGCSIPEDRYYWPEKHVWARPEPDGTVTIGITDVAQHLAKTIISVTPRGVGRKVQR
CCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHC
GKSAGTLESGKWVGPVTSPISGEIVAVNDAAIADPKLLNQDPYGAGWFARLRPDDWENES
CCCCCCCCCCCEECCCCCCCCCCEEEECCCCCCCCHHCCCCCCCCCEEEEECCCCCCCCC
KLLVTGPEAVEAYRKVLEQEGISCS
EEEEECHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]