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The map label for this gene is gcvH2 [H]

Identifier: 221632177

GI number: 221632177

Start: 154253

End: 154717

Strand: Reverse

Name: gcvH2 [H]

Synonym: trd_0143

Alternate gene names: 221632177

Gene position: 154717-154253 (Counterclockwise)

Preceding gene: 221632178

Following gene: 221632176

Centisome position: 7.72

GC content: 66.24

Gene sequence:

>465_bases
GTGAGCTTTGCCGAAACGACTCGCGTCTATCGCGGCTGCGTCATCCCGCTCGACCTGCTCTACGATGTCGAGCGCGACGT
CTGGGTGCGCCGAGAGGGAGAATTGGCCACGCTCGGAATGACCGATCCAGCCCAGACGCGAGCAGGCAAACTGGTGGCCA
TTCGCTTCCGCCGACCCGGCACCATCGTCGAGCGCGGGCGTGCGCTGGCCACGATCGAGAGCGGGAAATGGGTCGGCCCC
TTCCCGGCCCCCTTCCGCTGCGAACTCGTCGAGACGAACCAGGCAGCCTTCGAGCGCGACATCCTGATCGCCAACCGCGA
CCCCTACGGTGAAGGCTGGCTCGTGCGAGTCCGCCCGCTCGCCCCTGAGGAACTCGCTGAACTCGTCACCGGCGAGGAGG
CGTTCACCCGCTATCGTGCGCGGATCGATGCGTTGGATCTCAACTGCTTCCGCTGTGCCGAGTGA

Upstream 100 bases:

>100_bases
GGCCGGATGACTGGGAAAACGAGTCGAAACTCTTGGTAACCGGTCCGGAGGCCGTCGAAGCCTACCGGAAGGTCTTGGAA
CAGGAAGGGATTTCCTGCTC

Downstream 100 bases:

>100_bases
CTGAAAGGAGGAAACGGTGCGTGTCCGCGTCGTCGATCTCGGTCTGGTCGACCCAATCCGCTCCCAGAGTGTCTACCACG
CCGTCGGGTACACCTTCCGA

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MSFAETTRVYRGCVIPLDLLYDVERDVWVRREGELATLGMTDPAQTRAGKLVAIRFRRPGTIVERGRALATIESGKWVGP
FPAPFRCELVETNQAAFERDILIANRDPYGEGWLVRVRPLAPEELAELVTGEEAFTRYRARIDALDLNCFRCAE

Sequences:

>Translated_154_residues
MSFAETTRVYRGCVIPLDLLYDVERDVWVRREGELATLGMTDPAQTRAGKLVAIRFRRPGTIVERGRALATIESGKWVGP
FPAPFRCELVETNQAAFERDILIANRDPYGEGWLVRVRPLAPEELAELVTGEEAFTRYRARIDALDLNCFRCAE
>Mature_153_residues
SFAETTRVYRGCVIPLDLLYDVERDVWVRREGELATLGMTDPAQTRAGKLVAIRFRRPGTIVERGRALATIESGKWVGPF
PAPFRCELVETNQAAFERDILIANRDPYGEGWLVRVRPLAPEELAELVTGEEAFTRYRARIDALDLNCFRCAE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 17474; Mature: 17343

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFAETTRVYRGCVIPLDLLYDVERDVWVRREGELATLGMTDPAQTRAGKLVAIRFRRPG
CCHHHHHHHHCCEEEEHHHEEECCCCEEEEECCCEEEECCCCCCCCCCCCEEEEEECCCC
TIVERGRALATIESGKWVGPFPAPFRCELVETNQAAFERDILIANRDPYGEGWLVRVRPL
CHHHCCCEEEEECCCCEECCCCCCEEEEEEECCCCHHCCEEEEECCCCCCCCEEEEEECC
APEELAELVTGEEAFTRYRARIDALDLNCFRCAE
CHHHHHHHHCCHHHHHHHHHHHEEECCCEEEECC
>Mature Secondary Structure 
SFAETTRVYRGCVIPLDLLYDVERDVWVRREGELATLGMTDPAQTRAGKLVAIRFRRPG
CHHHHHHHHCCEEEEHHHEEECCCCEEEEECCCEEEECCCCCCCCCCCCEEEEEECCCC
TIVERGRALATIESGKWVGPFPAPFRCELVETNQAAFERDILIANRDPYGEGWLVRVRPL
CHHHCCCEEEEECCCCEECCCCCCEEEEEEECCCCHHCCEEEEECCCCCCCCEEEEEECC
APEELAELVTGEEAFTRYRARIDALDLNCFRCAE
CHHHHHHHHCCHHHHHHHHHHHEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]