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The map label for this gene is potD-A [H]

Identifier: 221632154

GI number: 221632154

Start: 125806

End: 126990

Strand: Reverse

Name: potD-A [H]

Synonym: trd_0119

Alternate gene names: 221632154

Gene position: 126990-125806 (Counterclockwise)

Preceding gene: 221632155

Following gene: 221632153

Centisome position: 6.34

GC content: 64.39

Gene sequence:

>1185_bases
ATGGCTGATCGGATCGATGCCCTGCTCCAGTTCCTGGCGCGCCAGCAGCGCCTCCGCCGCCGCGAGCTACTCGGTGGCCT
CCTGGCAGCCGGCACGCTCGCTGCCTGCCGGCAGGCGGCCACTCCGACACCGACTCCGGCCCCAGCCGCGACACCGACGC
CTGCTGCCCAGGCGCCGGCGGGTGAATTCGCGGTTCCTGGCTATGACGATCCCAACCGCTGGAAGGGCCGCAGTATCGTC
GTCACCTCCTGGGGCGGCGCTCTGCAGGATGCCCTGCGCAAGACGATCTACCAGCCCTTCTCGCGTCTCACCGGCTGTCA
GATCGTCGAAGATACGACCGACGAGGCCAAGCTGCGAACCATGGTCGAGTCCGGCACTGTCGAGTGGGACGTGGTCGATG
TCGGGACCGAATCGGTCATCCCGATGGGTCGCCTCAACCTGCTGGAGCCACTCGACTACAGCAAGATCGATACGACCGAT
ATCTTTCCGGAACTCGTGCTCGAACACGGCGTCGGTTACTTCTATTACTCGACGTGTCTCGCCTACCGGAAGGACAAGTT
CCCGGACAAGCCGCCCCAGAGCTGGGCCGATTTCTGGGATGTGCAAGGTTTCCCTGGGCCGCGTGCATTCCAGAAGTACA
TCCAGTGGGGACCGCTCGAGGCAGCCCTCCTGGCCGACGGAGTACCGATCGACCAACTCTACCCGCCGGATGTCGACCGT
GCCTTCCGTGCACTCGACCGCCTCAAGCCACACGTGACTGTCTGGTGGGAGGCGGGTGCCCAACCGGCTCAACTCCTCAC
CGATGGCGAGGTCTATCTCACCGATGCCTGGATCGCCCGCATCCAGTTCTTGATCGAGCAAGGTTCCACGGTCCTCGGTT
ACACGTGGAACCAGGGACGGCTCTCGAGCGACTCGCTGGTCATCCCGCGCGGCTCCAAGAACGTGGATGTCGCCCACGAC
TTCATCAACTTCGCCTTGCGTCCGGAGGTACAGCGGGCCTTCGCGCTGGCCTATCCGGACGGCCCAGCCAACAAGCGGGC
GTTCGAAGCCTTGCCCGCAGAACGCGTGGCCGTGTTGCCGAGTGCGCCACAGAACAAGGCGCTCCAGGTCTATCCGGACT
ATCAGTGGTGGGCCGATCACCTCGACGAGGTCGTCGAGCGCTTCAATAGCTGGATCGCTCGCTAG

Upstream 100 bases:

>100_bases
GCCGCTTCCAGGAACCTGGCAAAGTGCGCGATGCCATCGCGCTTCCTTTCCCGACCCGTTGAGAGCACTGTGCATGGCTC
AGACGAGGAGGAGGTGTACC

Downstream 100 bases:

>100_bases
CACCATCGTCCAGCGCTGCAGGTACGCCGGACACCACCTGGCTTCGGTGTCCGGCGTACCGCTGGCTGGAACGGATCCAC
CTACGGGGTCGATACACCGC

Product: putative transport system periplasmic protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MADRIDALLQFLARQQRLRRRELLGGLLAAGTLAACRQAATPTPTPAPAATPTPAAQAPAGEFAVPGYDDPNRWKGRSIV
VTSWGGALQDALRKTIYQPFSRLTGCQIVEDTTDEAKLRTMVESGTVEWDVVDVGTESVIPMGRLNLLEPLDYSKIDTTD
IFPELVLEHGVGYFYYSTCLAYRKDKFPDKPPQSWADFWDVQGFPGPRAFQKYIQWGPLEAALLADGVPIDQLYPPDVDR
AFRALDRLKPHVTVWWEAGAQPAQLLTDGEVYLTDAWIARIQFLIEQGSTVLGYTWNQGRLSSDSLVIPRGSKNVDVAHD
FINFALRPEVQRAFALAYPDGPANKRAFEALPAERVAVLPSAPQNKALQVYPDYQWWADHLDEVVERFNSWIAR

Sequences:

>Translated_394_residues
MADRIDALLQFLARQQRLRRRELLGGLLAAGTLAACRQAATPTPTPAPAATPTPAAQAPAGEFAVPGYDDPNRWKGRSIV
VTSWGGALQDALRKTIYQPFSRLTGCQIVEDTTDEAKLRTMVESGTVEWDVVDVGTESVIPMGRLNLLEPLDYSKIDTTD
IFPELVLEHGVGYFYYSTCLAYRKDKFPDKPPQSWADFWDVQGFPGPRAFQKYIQWGPLEAALLADGVPIDQLYPPDVDR
AFRALDRLKPHVTVWWEAGAQPAQLLTDGEVYLTDAWIARIQFLIEQGSTVLGYTWNQGRLSSDSLVIPRGSKNVDVAHD
FINFALRPEVQRAFALAYPDGPANKRAFEALPAERVAVLPSAPQNKALQVYPDYQWWADHLDEVVERFNSWIAR
>Mature_393_residues
ADRIDALLQFLARQQRLRRRELLGGLLAAGTLAACRQAATPTPTPAPAATPTPAAQAPAGEFAVPGYDDPNRWKGRSIVV
TSWGGALQDALRKTIYQPFSRLTGCQIVEDTTDEAKLRTMVESGTVEWDVVDVGTESVIPMGRLNLLEPLDYSKIDTTDI
FPELVLEHGVGYFYYSTCLAYRKDKFPDKPPQSWADFWDVQGFPGPRAFQKYIQWGPLEAALLADGVPIDQLYPPDVDRA
FRALDRLKPHVTVWWEAGAQPAQLLTDGEVYLTDAWIARIQFLIEQGSTVLGYTWNQGRLSSDSLVIPRGSKNVDVAHDF
INFALRPEVQRAFALAYPDGPANKRAFEALPAERVAVLPSAPQNKALQVYPDYQWWADHLDEVVERFNSWIAR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=261, Percent_Identity=25.6704980842912, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 44014; Mature: 43883

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRIDALLQFLARQQRLRRRELLGGLLAAGTLAACRQAATPTPTPAPAATPTPAAQAPA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GEFAVPGYDDPNRWKGRSIVVTSWGGALQDALRKTIYQPFSRLTGCQIVEDTTDEAKLRT
CCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHH
MVESGTVEWDVVDVGTESVIPMGRLNLLEPLDYSKIDTTDIFPELVLEHGVGYFYYSTCL
HHHCCCEEEEEEECCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
AYRKDKFPDKPPQSWADFWDVQGFPGPRAFQKYIQWGPLEAALLADGVPIDQLYPPDVDR
HHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCHHH
AFRALDRLKPHVTVWWEAGAQPAQLLTDGEVYLTDAWIARIQFLIEQGSTVLGYTWNQGR
HHHHHHHCCCCEEEEECCCCCHHHHCCCCCEEEHHHHHHHHHHHHHCCCEEEEEEECCCC
LSSDSLVIPRGSKNVDVAHDFINFALRPEVQRAFALAYPDGPANKRAFEALPAERVAVLP
CCCCCEEECCCCCCCHHHHHHHHHHCCHHHHHHEEEECCCCCCCCHHHHHCCHHHEEECC
SAPQNKALQVYPDYQWWADHLDEVVERFNSWIAR
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADRIDALLQFLARQQRLRRRELLGGLLAAGTLAACRQAATPTPTPAPAATPTPAAQAPA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GEFAVPGYDDPNRWKGRSIVVTSWGGALQDALRKTIYQPFSRLTGCQIVEDTTDEAKLRT
CCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHH
MVESGTVEWDVVDVGTESVIPMGRLNLLEPLDYSKIDTTDIFPELVLEHGVGYFYYSTCL
HHHCCCEEEEEEECCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
AYRKDKFPDKPPQSWADFWDVQGFPGPRAFQKYIQWGPLEAALLADGVPIDQLYPPDVDR
HHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCHHH
AFRALDRLKPHVTVWWEAGAQPAQLLTDGEVYLTDAWIARIQFLIEQGSTVLGYTWNQGR
HHHHHHHCCCCEEEEECCCCCHHHHCCCCCEEEHHHHHHHHHHHHHCCCEEEEEEECCCC
LSSDSLVIPRGSKNVDVAHDFINFALRPEVQRAFALAYPDGPANKRAFEALPAERVAVLP
CCCCCEEECCCCCCCHHHHHHHHHHCCHHHHHHEEEECCCCCCCCHHHHHCCHHHEEECC
SAPQNKALQVYPDYQWWADHLDEVVERFNSWIAR
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]