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The map label for this gene is ligT [C]

Identifier: 221632128

GI number: 221632128

Start: 103477

End: 104052

Strand: Reverse

Name: ligT [C]

Synonym: trd_0093

Alternate gene names: 221632128

Gene position: 104052-103477 (Counterclockwise)

Preceding gene: 221632134

Following gene: 221632127

Centisome position: 5.19

GC content: 65.45

Gene sequence:

>576_bases
ATGCGTGAAGAGTGGCGGCTTTTTGCCGCCATACCGGTTCCAGATGATGTGCGCCAGCGGGTCGCCGCGGCTATCGCGCA
TCTGCAAGCACGCGGGTACCGTGCCAAGTGGGTCGATCCGGAGGCAACGCACCTCACTGTCAAGTTCTTCGGATCGGTCG
CGACGGATAGCCTGCCGGACCTCACGCGCGCGCTGCGTAAAAGCGTGACCGGTCTTGCTCGCTTCGAGCTCCAGGTGCAT
GGTGCGGGGGCGTTCCCACACCCACTGCGCCCGCGGGTCCTCTGGCTCGGCATCGATGGGGAGACGGCGCCGCTCGCTCA
GCTCGTCGAGCAGGTCGAGCGTTCCGCTGGTTCCTTTGCTGAAAAGACCGACGAGCGGCCATTCCATCCGCATCTCACGC
TTGCCCGCGTCCGGCCGGAGGACTTGGCGACGCTGCGTGCCCTGCCGCAGGAACTCGAGCGACTCGCTCGATTGCCGCCG
CTCCCGTTCACCGTCGACCGGCTGACGTTGTATCGGAGCGAGCTCTTCCGTTCCGGGCCTCGCTACACCATCATTGAGGA
GTTCCTGCTCCGATGA

Upstream 100 bases:

>100_bases
CAGCCGAGAGCCAGCGCCTACCCCAGCATGCTCAGGCACCACAAAGCTGCGATTGGTATACTGAAAAACGAGAACGATGA
GGCCACGAGGTCGTGCCGCC

Downstream 100 bases:

>100_bases
AATCAGCAAGACACGTTGCGGTCTGCGGCCCGCACGAGGCGACACCGGAGGAACAGGCACTGGCCGAACGCCTGGGAGAA
CTCCTGGCCGTTTCTGGTCT

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MREEWRLFAAIPVPDDVRQRVAAAIAHLQARGYRAKWVDPEATHLTVKFFGSVATDSLPDLTRALRKSVTGLARFELQVH
GAGAFPHPLRPRVLWLGIDGETAPLAQLVEQVERSAGSFAEKTDERPFHPHLTLARVRPEDLATLRALPQELERLARLPP
LPFTVDRLTLYRSELFRSGPRYTIIEEFLLR

Sequences:

>Translated_191_residues
MREEWRLFAAIPVPDDVRQRVAAAIAHLQARGYRAKWVDPEATHLTVKFFGSVATDSLPDLTRALRKSVTGLARFELQVH
GAGAFPHPLRPRVLWLGIDGETAPLAQLVEQVERSAGSFAEKTDERPFHPHLTLARVRPEDLATLRALPQELERLARLPP
LPFTVDRLTLYRSELFRSGPRYTIIEEFLLR
>Mature_191_residues
MREEWRLFAAIPVPDDVRQRVAAAIAHLQARGYRAKWVDPEATHLTVKFFGSVATDSLPDLTRALRKSVTGLARFELQVH
GAGAFPHPLRPRVLWLGIDGETAPLAQLVEQVERSAGSFAEKTDERPFHPHLTLARVRPEDLATLRALPQELERLARLPP
LPFTVDRLTLYRSELFRSGPRYTIIEEFLLR

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 21702; Mature: 21702

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREEWRLFAAIPVPDDVRQRVAAAIAHLQARGYRAKWVDPEATHLTVKFFGSVATDSLPD
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHCCCCHH
LTRALRKSVTGLARFELQVHGAGAFPHPLRPRVLWLGIDGETAPLAQLVEQVERSAGSFA
HHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCHHH
EKTDERPFHPHLTLARVRPEDLATLRALPQELERLARLPPLPFTVDRLTLYRSELFRSGP
HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
RYTIIEEFLLR
CHHHHHHHHCC
>Mature Secondary Structure
MREEWRLFAAIPVPDDVRQRVAAAIAHLQARGYRAKWVDPEATHLTVKFFGSVATDSLPD
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHCCCCHH
LTRALRKSVTGLARFELQVHGAGAFPHPLRPRVLWLGIDGETAPLAQLVEQVERSAGSFA
HHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCHHH
EKTDERPFHPHLTLARVRPEDLATLRALPQELERLARLPPLPFTVDRLTLYRSELFRSGP
HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
RYTIIEEFLLR
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]