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The map label for this gene is amiA [H]

Identifier: 221232628

GI number: 221232628

Start: 1851811

End: 1853790

Strand: Reverse

Name: amiA [H]

Synonym: SPN23F_19100

Alternate gene names: 221232628

Gene position: 1853790-1851811 (Counterclockwise)

Preceding gene: 221232629

Following gene: 221232627

Centisome position: 83.45

GC content: 38.94

Gene sequence:

>1980_bases
ATGAAAAAAAATCGTGTATTTGCTACAGCAGGTCTTGTTTTATTAGCAGCAGGTGTACTTGCAGCATGCAGTTCTTCAAA
ATCATCTGATTCATCAGCCCCTAAAGCTTATGGCTATGTTTATACAGCAGACCCAGAAACCTTGGACTACCTGATTTCAA
GTAAAAATAGTACAACAGTAGTGACTTCAAATGGGATTGATGGTTTATTCACTAACGATAATTACGGTAATCTTGCTCCT
GCAGTTGCAGAGGATTGGGAAGTCTCTAAGGATGGTTTGACCTACACTTATAAGATTCGTAAAGGGGTTAAATGGTTTAC
CTCTGATGGAGAAGAATATGCAGAGGTGACGGCTAAAGATTTCGTGAACGGTTTAAAACACGCAGCAGATAAAAAATCAG
AAGCTATGTATTTAGCTGAAAATTCGGTTAAAGGCTTGGCAGATTATCTATCAGGAACTTCAACAGATTTTTCAACAGTT
GGTGTCAAGGCGGTTGATGATTATACGTTACAATACACTTTGAACCAGCCTGAACCGTTCTGGAACTCTAAGTTGACCTA
TTCTATTTTCTGGCCTCTGAATGAAGAATTCGAAACATCAAAAGGAAGCGATTTTGCTAAACCAACAGATCCGACATCCT
TGCTTTATAATGGTCCATTCTTGTTGAAAGGGTTGACTGCAAAATCTTCTGTAGAGTTTGTAAAAAATGAGCAATATTGG
GATAAAGAAAATGTCCACCTAGATACTATCAATCTAGCTTACTATGATGGATCAGATCAGGAGTCGCTAGAGCGTAACTT
CACTAGTGGAGCTTATAGTTATGCCCGTCTTTACCCTACCAGCTCCAACTATTCTAAGGTTGCAGAAGAATACAAGGACA
ATATCTATTACACACAATCAGGCTCTGGGATTGCTGGTCTGGGTGTGAATATTGATCGCCAAAGTTACAACTATACTTCT
AAAACTACAGATTCAGAGAAAGTAGCTACTAAGAAGGCATTGCTTAACAAAGATTTCCGTCAAGCCTTGAATTTTGCTCT
TGATCGCTCAGCTTACTCAGCTCAAATCAATGGTAAAGATGGAGCAGCTTTAGCAGTTCGTAATTTATTTGTAAAACCAG
ACTTTGTTTCAGCTGGTGAGAAGACCTTTGGTGATTTAGTCGCTGCTCAACTTCCTGCTTATGGTGATGAGTGGAAAGGT
GTGAATTTAGCTGATGGGCAGGATGGTTTATTCAATGCTGACAAGGCCAAGGCAGAGTTTGCGAAAGCTAAGAAAGCTTT
AGAAGCAGACGGCGTTCAGTTTCCTATTCATCTGGACGTTCCAGTAGACCAAGCATCAAAAAACTATATATCTCGTATTC
AGTCCTTTAAACAATCTGTAGAAACAGTTCTTGGTGTTGAAAATGTCGTTGTTGATATTCAACAAATGACAAGTGATGAA
TTCCTTAATATTACTTACTATGCTGCCAATGCTTCATCTGAGGATTGGGATGTATCAGGAGGAGTTTCATGGGGGCCAGA
CTATCAAGACCCATCTACTTACCTGGATATTTTAAAAACAACTAGCAGTGAAACTACAAAAACATATTTAGGATTTGATA
ATCCAAATAGCCCTTCAGTAGTTCAAGTTGGTTTGAAAGAATACGATAAATTAGTTGATGAAGCTGCCAGAGAGACAAGC
GACTTGAATGTCCGTTATGAAAAATATGCAGCGGCTCAAGCATGGTTGACAGATAGTTCACTCTTTATTCCTGCTATGGC
TTCTTCTGGTGCAGCACCAGTGCTTTCACGAATTGTTCCATTTACTGGAGCTTCTGCGCAAACAGGCTCTAAGGGGTCAG
ATGTTTACTTCAAATATTTGAAATTACAAGATAAAGTGGTGACTAAGGAAGAGTATGAAAAAGCTCGTGAAAAATGGTTG
AAAGAAAAAGCTGAATCAAATGAGAAAGCTCAAAAAGAATTGGCAAGTCATGTGAAGTAA

Upstream 100 bases:

>100_bases
GCCAACAAGCCTAAATATTCTGAAAATTTGTTTGATTTTTAATGGATAATGTGATATAATGGTTGTCAATCTATAATTCA
CATAAACAGAGGAGTAGAAA

Downstream 100 bases:

>100_bases
ATAATTTCTAATAGAACTTTCTCTCCATGAGGAGAAGGTTCTTTTGGGATTTTTAAAGGAAATAATATGAAAAAATATAT
TTTTATGCGTGTTTTGCGGT

Product: extracellular oligopeptide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTADPETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAP
AVAEDWEVSKDGLTYTYKIRKGVKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNGPFLLKGLTAKSSVEFVKNEQYW
DKENVHLDTINLAYYDGSDQESLERNFTSGAYSYARLYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTS
KTTDSEKVATKKALLNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLPAYGDEWKG
VNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYISRIQSFKQSVETVLGVENVVVDIQQMTSDE
FLNITYYAANASSEDWDVSGGVSWGPDYQDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETS
DLNVRYEKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQDKVVTKEEYEKAREKWL
KEKAESNEKAQKELASHVK

Sequences:

>Translated_659_residues
MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTADPETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAP
AVAEDWEVSKDGLTYTYKIRKGVKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNGPFLLKGLTAKSSVEFVKNEQYW
DKENVHLDTINLAYYDGSDQESLERNFTSGAYSYARLYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTS
KTTDSEKVATKKALLNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLPAYGDEWKG
VNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYISRIQSFKQSVETVLGVENVVVDIQQMTSDE
FLNITYYAANASSEDWDVSGGVSWGPDYQDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETS
DLNVRYEKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQDKVVTKEEYEKAREKWL
KEKAESNEKAQKELASHVK
>Mature_659_residues
MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTADPETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAP
AVAEDWEVSKDGLTYTYKIRKGVKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNGPFLLKGLTAKSSVEFVKNEQYW
DKENVHLDTINLAYYDGSDQESLERNFTSGAYSYARLYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTS
KTTDSEKVATKKALLNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLPAYGDEWKG
VNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYISRIQSFKQSVETVLGVENVVVDIQQMTSDE
FLNITYYAANASSEDWDVSGGVSWGPDYQDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETS
DLNVRYEKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQDKVVTKEEYEKAREKWL
KEKAESNEKAQKELASHVK

Specific function: Part of the binding-protein-dependent transport system for oligopeptides; probably an oligopeptide binding protein [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789397, Length=248, Percent_Identity=30.241935483871, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1787495, Length=235, Percent_Identity=28.936170212766, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1789966, Length=532, Percent_Identity=24.6240601503759, Blast_Score=83, Evalue=7e-17,
Organism=Escherichia coli, GI87081878, Length=589, Percent_Identity=22.9202037351443, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 72492; Mature: 72492

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTADPETLDYLISSKNSTTV
CCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCEEE
VTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKGVKWFTSDGEEYAEVTAKD
EECCCCCCEEECCCCCCCCCCHHCCCCCCCCCCEEEEEECCCCEEECCCCHHHHHHHHHH
FVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTVGVKAVDDYTLQYTLNQPEPF
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCHHCCEEEECCEEEEEEECCCCCC
WNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNGPFLLKGLTAKSSVEFVKNEQYW
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCHHHHHHHCCCCC
DKENVHLDTINLAYYDGSDQESLERNFTSGAYSYARLYPTSSNYSKVAEEYKDNIYYTQS
CCCCCEEEEEEEEEECCCCHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEC
GSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKALLNKDFRQALNFALDRSAYSAQINGKD
CCCEEEECEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
GAALAVRNLFVKPDFVSAGEKTFGDLVAAQLPAYGDEWKGVNLADGQDGLFNADKAKAEF
CCEEEEHHHEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
AKAKKALEADGVQFPIHLDVPVDQASKNYISRIQSFKQSVETVLGVENVVVDIQQMTSDE
HHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FLNITYYAANASSEDWDVSGGVSWGPDYQDPSTYLDILKTTSSETTKTYLGFDNPNSPSV
EEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCE
VQVGLKEYDKLVDEAARETSDLNVRYEKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVP
EEECHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHCC
FTGASAQTGSKGSDVYFKYLKLQDKVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK
CCCCCCCCCCCCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTADPETLDYLISSKNSTTV
CCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCEEE
VTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKGVKWFTSDGEEYAEVTAKD
EECCCCCCEEECCCCCCCCCCHHCCCCCCCCCCEEEEEECCCCEEECCCCHHHHHHHHHH
FVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTVGVKAVDDYTLQYTLNQPEPF
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCHHCCEEEECCEEEEEEECCCCCC
WNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNGPFLLKGLTAKSSVEFVKNEQYW
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCHHHHHHHCCCCC
DKENVHLDTINLAYYDGSDQESLERNFTSGAYSYARLYPTSSNYSKVAEEYKDNIYYTQS
CCCCCEEEEEEEEEECCCCHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEC
GSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKALLNKDFRQALNFALDRSAYSAQINGKD
CCCEEEECEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
GAALAVRNLFVKPDFVSAGEKTFGDLVAAQLPAYGDEWKGVNLADGQDGLFNADKAKAEF
CCEEEEHHHEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
AKAKKALEADGVQFPIHLDVPVDQASKNYISRIQSFKQSVETVLGVENVVVDIQQMTSDE
HHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FLNITYYAANASSEDWDVSGGVSWGPDYQDPSTYLDILKTTSSETTKTYLGFDNPNSPSV
EEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCE
VQVGLKEYDKLVDEAARETSDLNVRYEKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVP
EEECHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHCC
FTGASAQTGSKGSDVYFKYLKLQDKVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK
CCCCCCCCCCCCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2352474; 11463916; 2684766; 3208757 [H]