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The map label for this gene is metB [H]

Identifier: 221232237

GI number: 221232237

Start: 1442763

End: 1443857

Strand: Reverse

Name: metB [H]

Synonym: SPN23F_14890

Alternate gene names: 221232237

Gene position: 1443857-1442763 (Counterclockwise)

Preceding gene: 221232238

Following gene: 221232236

Centisome position: 65.0

GC content: 44.11

Gene sequence:

>1095_bases
ATGAGCAAGGAATTACACATTAACACAATTTTAGCACAAGCAGGGATCAAGTCTGATGAGGCAACAGGTGCTTTGGTGAC
ACCACTTCATTTTTCAACGACCTATCAGCATCCAGAGTTTGGTCGATCTACTGGGTTTGACTATACGCGCACTAAAAATC
CAACTCGTAGTAAGGCTGAGGAAGTCTTGGCGGCTATTGAGTCAGCAGACTATGCCCTAGCGACTAGCTCAGGGATGTCA
GCTATTGTACTGGCCTTTAGCGTCTTTCCAGTAGGAAGTAAGGTCTTGGCAGTGCGTGATCTTTACGGTGGTTCTTTTCG
CTGGTTTAACCAAGTGGAGCAGGAAGGTCATTTCCATTTTACCTATGCCAATACAGAAGAAGAGTTGATTGCCGAGTTAG
AAAAGGATGTGGATGTTCTCTATATCGAAACCCCAACCAATCCCTTGATGTTGGAATTTGATATCGAAAAACTAGCAAAA
TTGGCTCATGCTAAGGGTGCCAAAGTGGTGGTGGACAATACCTTCTATAGCCCTATCTACCAACGTCCGATTGAAGATGG
AGCAGATATCGTTCTCCATTCAGCAACCAAGTATCTAGCAGGCCACAATGATGTCTTGGCTGGAGTGGTTGTGACCAATA
GTTTAGAACTATACGAGAAGCTTTTTTACAATCTTAATACAACAGGGGCAGTCTTGTCTCCATTTGACAGCTACCAGTTG
CTTCGTGGTCTCAAGACCTTGTCTCTTCGTATGGAGCGCTCAACAGCTAACGCCCAAGAAGTGGTTGCCTTTTTGAAGGA
TTCTCCAGCAGTTAAGGAAGTTCTCTACACTGGTCGTGGAGGCATGATTTCCTTTAAAGTAGCCGATGAAACACGCATTC
CTCATATTTTGAACAGTCTCAAGGTCTTCTCTTTTGCGGAAAGTTTGGGTGGAGTGGAAAGTCTTATTACTTATCCAACG
ACTCAAACTCATGCTGATATTCCAGCAGAAGTACGCCATTCTTATGGTTTGACAGATGACCTCTTGCGTTTGTCTATTGG
GATTGAGGATGCTAGAGATTTGATTGCAGATTTGCGCCAAGCCTTAGAAGGATAA

Upstream 100 bases:

>100_bases
CTTTTTATATAGTTTGAAACTATAACTAGCTCTTGAAAAGAAGAAAATGAGTTGATGAAAATAAGTGGTACAATAGTTAC
TATAGATTTGGAGGAATTGT

Downstream 100 bases:

>100_bases
GACAAAGATGGGAAAATATGATTTTACAAGCCTGCCCAACCGTTTAGGGCACCATACCTATAAATGGAAAGAAACAGAAA
CGGATAGTGAAGTTCTACCA

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-acetylhomoserine (thiol)-lyase; O-acetylhomoserine sulfhydrylase; OAH sulfhydrylase [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRSTGFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMS
AIVLAFSVFPVGSKVLAVRDLYGGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELYEKLFYNLNTTGAVLSPFDSYQL
LRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLYTGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPT
TQTHADIPAEVRHSYGLTDDLLRLSIGIEDARDLIADLRQALEG

Sequences:

>Translated_364_residues
MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRSTGFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMS
AIVLAFSVFPVGSKVLAVRDLYGGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELYEKLFYNLNTTGAVLSPFDSYQL
LRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLYTGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPT
TQTHADIPAEVRHSYGLTDDLLRLSIGIEDARDLIADLRQALEG
>Mature_363_residues
SKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRSTGFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSA
IVLAFSVFPVGSKVLAVRDLYGGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAKL
AHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELYEKLFYNLNTTGAVLSPFDSYQLL
RGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLYTGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTT
QTHADIPAEVRHSYGLTDDLLRLSIGIEDARDLIADLRQALEG

Specific function: Catalyzes the transformation of O-succinylhomoserine into cystathionine, as well as O-acetylhomoserine and sulfide into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=360, Percent_Identity=40.2777777777778, Blast_Score=251, Evalue=5e-67,
Organism=Homo sapiens, GI299473759, Length=379, Percent_Identity=36.4116094986807, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI25453487, Length=357, Percent_Identity=35.8543417366947, Blast_Score=196, Evalue=2e-50,
Organism=Escherichia coli, GI1790375, Length=370, Percent_Identity=41.8918918918919, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1789383, Length=321, Percent_Identity=25.8566978193146, Blast_Score=104, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17538055, Length=362, Percent_Identity=39.5027624309392, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI17560056, Length=362, Percent_Identity=37.8453038674033, Blast_Score=227, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI71980708, Length=390, Percent_Identity=33.3333333333333, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6319307, Length=367, Percent_Identity=39.5095367847411, Blast_Score=258, Evalue=9e-70,
Organism=Saccharomyces cerevisiae, GI6321254, Length=401, Percent_Identity=31.1720698254364, Blast_Score=186, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=31.4087759815243, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321904, Length=384, Percent_Identity=29.4270833333333, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6321170, Length=335, Percent_Identity=25.0746268656716, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922554, Length=363, Percent_Identity=41.3223140495868, Blast_Score=269, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48; =2.5.1.49 [H]

Molecular weight: Translated: 40104; Mature: 39973

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRSTGFDYTRTKNPTRSKAE
CCCCEEHHHHHHHCCCCCCCCCCEEEECEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
EVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLYGGSFRWFNQVEQEGHFHF
HHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEE
TYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAKLAHAKGAKVVVDNTFYSPIY
EECCCHHHHHHHHHCCCEEEEEECCCCCEEEEECHHHHHHHHHCCCCEEEEECCCCCHHH
QRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELYEKLFYNLNTTGAVLSPFDSYQL
HCCCCCCCCEEEEHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCCCCCCEECCCHHHHH
LRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLYTGRGGMISFKVADETRIPHILNSL
HHHHHHHHHEEHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
KVFSFAESLGGVESLITYPTTQTHADIPAEVRHSYGLTDDLLRLSIGIEDARDLIADLRQ
HHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHH
ALEG
HHCC
>Mature Secondary Structure 
SKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRSTGFDYTRTKNPTRSKAE
CCCEEHHHHHHHCCCCCCCCCCEEEECEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
EVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLYGGSFRWFNQVEQEGHFHF
HHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEE
TYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAKLAHAKGAKVVVDNTFYSPIY
EECCCHHHHHHHHHCCCEEEEEECCCCCEEEEECHHHHHHHHHCCCCEEEEECCCCCHHH
QRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELYEKLFYNLNTTGAVLSPFDSYQL
HCCCCCCCCEEEEHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCCCCCCEECCCHHHHH
LRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLYTGRGGMISFKVADETRIPHILNSL
HHHHHHHHHEEHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
KVFSFAESLGGVESLITYPTTQTHADIPAEVRHSYGLTDDLLRLSIGIEDARDLIADLRQ
HHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHH
ALEG
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]