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The map label for this gene is glyS [H]

Identifier: 221232199

GI number: 221232199

Start: 1395933

End: 1397969

Strand: Reverse

Name: glyS [H]

Synonym: SPN23F_14390

Alternate gene names: 221232199

Gene position: 1397969-1395933 (Counterclockwise)

Preceding gene: 221232200

Following gene: 221232198

Centisome position: 62.93

GC content: 41.83

Gene sequence:

>2037_bases
ATGACAAAAAACTTATTAGTAGAACTCGGTCTTGAAGAATTACCAGCCTATGTTGTTACGCCAAGTGAAAAACAACTAGG
CGAAAAAATGGCAGCCTTCCTCAAGGGAAAACGCCTGTCTTTTGAAGCCATTCAAACTTTCTCAACACCACGTCGTTTGG
CTGTTCGTGTAACTGGTCTTGCAGACAAACAGTCTGATTTAACAGAAGATTTCAAGGGTCCAGCAAAGAAAATTGCCTTA
GATAGTGATGGAAACTTCACCAAAGCAGCTCAAGGATTTGTCCGTGGGAAAGGTTTGACTGTTGAAGATATCGAATTCCG
TGAAATCAAGGGTGAAGAATATGTCTATGTCACTAAGGAAGAAATTGGTCAAGCAGTTGAAGCCATTGTTCCAGGCATTG
TGGATGTCTTGAAGTCACTGACTTTCCCTGTCAGCATGCACTGGGCGGGAAATAGCTTTGAATACATCCGCCCTGTTCAC
ACTTTAACTGTTCTCTTGGATGAGCAAGAGTTTGACTTGGATTTCCTTGATATCAAGGGAAGTCGTGTGAGTCGTGGCCA
TCGTTTTTTGGGACAAGAAACCAAGATTCAGTCAGCATTGAGCTATGAAGAAGACCTTCGTAAGCAGTTTGTAATCGCAG
ATCCATGTGAACGTGAGCAAATGATTGTTGACCAAATCAAGGAAATTGAGGCAAAACATGGTGTACGTATCGAAATTGAT
GCGGATTTGCTGAATGAAGTCTTGAATTTGGTTGAATACCCAACTGCCTTCATGGGAAGTTTTGATGCTAAATACCTTGA
AGTTCCAGAAGAAGTCTTGGTGACTTCTATGAAGGAACACCAGCGTTACTTTGTTGTTCGTGATCAAGATGGAAAACTCT
TGCCAAACTTCATTTCTGTTCGTAACGGAAACGCAGAGCGTTTGAAAAATGTCATCAAAGGAAATGAAAAAGTCTTGGTA
GCCCGCTTGGAAGACGGAGAATTCTTCTGGCGTGAAGACCAAAAATTGGTGATTTCAGATCTTGTTGAAAAATTAAACAA
TGTCACCTTCCATGAGAAGATTGGTTCCCTTCGTGAACACATGATTCGTACGGGTCAAATCACTGTACTTTTGGCAGAAA
AAGCTAGTTTGTCAGTGGATGAAACAGTTGACCTTGCTCGTGCAGCAGCCATTTACAAGTTTGACTTGTTGACAGGTATG
GTTGGTGAATTTGACGAACTCCAAGGAATTATGGGTGAAAAATACACCCTTCTTGCTGGTGAAACTCCAGCGGTGGCAGC
TGCTATTCGTGAACACTACATGCCTACATCAGCTGAAGGAGAACTTCCAGAGAGCAAGGTCGGCGCAGTTCTAGCCATTG
CAGACAAATTGGATACGATTTTGAGTTTCTTCTCAGTAGGATTGATTCCATCAGGTTCTAATGACCCTTATGCCCTTCGT
CGTGCAACTCAAGGTGTGGTTCGTATCTTGGATGCCTTTGGTTGGCACATTGCTATGGATGAGCTGATTGATAGCCTTTA
TGCATTGAAATTTGACAGTTTGACTTATGAAAATAAAGCAGAGGTTATGGACTTTATCAAGGCTCGTGTTGATAAGATGA
TGGGCTCTACTCCAAAAGATATCAAGGAAGCAGTTCTTGCAGGTTCAAACTTTGTTGTGGCAGATATGTTGGAAGCAGCA
AGTGCTCTCGTAGAAGTAAGCAAGGAAGAAGATTTTAAACCATCTGTTGAATCACTTTCTCGTGCCTTTAACCTGGCCGA
GAAGGCAGAAGGGGTTGCTACGGTTGATTCAGCACTATTTGAGAATGACCAAGAAAAAGCTTTGGCAGAAGCAGTAGAAA
CACTCATTTTATCAGGACCTGCAAGTCAGCAATTGAAACAACTTTTTGCGCTTAGCCCAGTCATTGATGCTTTCTTTGAA
AATACTATGGTAATGGCTGAAGATCAGGCTGTCCGTCAAAATCGTTTGGCAATCTTGTCACAACTAACCAAGAAAGCAGC
TAAGTTTGCTTGTTTTAACCAAATTAACACTAAATAA

Upstream 100 bases:

>100_bases
AGCATTGGATTTCCATACAGACTCACAGAGTTAGTTAGCGACGTCCCCAGTACCTAAGGTGCATATCAAAAAAGATTGAA
GAAAATGTAAAGGAAAAAAC

Downstream 100 bases:

>100_bases
AATTTGATAAACGGACTTTATCTTATTACAAAGGAGAAGAAATGGATCCGAAAAAAATTGCTCGTATCAATGAGCTTGCT
AAAAAGAAAAAAACAGAAGG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAIQTFSTPRRLAVRVTGLADKQSDLTEDFKGPAKKIAL
DSDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVH
TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGQETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKEIEAKHGVRIEID
ADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLV
ARLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIYKFDLLTGM
VGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALR
RATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAA
SALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLILSGPASQQLKQLFALSPVIDAFFE
NTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK

Sequences:

>Translated_678_residues
MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAIQTFSTPRRLAVRVTGLADKQSDLTEDFKGPAKKIAL
DSDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVH
TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGQETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKEIEAKHGVRIEID
ADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLV
ARLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIYKFDLLTGM
VGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALR
RATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAA
SALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLILSGPASQQLKQLFALSPVIDAFFE
NTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK
>Mature_677_residues
TKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAIQTFSTPRRLAVRVTGLADKQSDLTEDFKGPAKKIALD
SDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVHT
LTVLLDEQEFDLDFLDIKGSRVSRGHRFLGQETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKEIEAKHGVRIEIDA
DLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVA
RLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIYKFDLLTGMV
GEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALRR
ATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAAS
ALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLILSGPASQQLKQLFALSPVIDAFFEN
TMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=676, Percent_Identity=35.6508875739645, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- InterPro:   IPR003607 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75440; Mature: 75309

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAIQTFSTPRRLAVRVTGL
CCCCCEEECCHHHCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEECC
ADKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKE
CCCHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHCCCCCEEECCHHHCCCCCCEEEEEHH
EIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVHTLTVLLDEQEFDLDFLDIKG
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHEEEEECCCCCCCEEEECCC
SRVSRGHRFLGQETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKEIEAKHGVRIEID
CHHHCCHHHCCCHHHHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHHHHHHHCCCEEEEC
ADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISV
HHHHHHHHHHHHCCHHHHCCCCCHHHHCCHHHHHHHHHHCCEEEEEECCCCCCCCHHHHC
RNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREH
CCCCHHHHHHHHCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
MIRTGQITVLLAEKASLSVDETVDLARAAAIYKFDLLTGMVGEFDELQGIMGEKYTLLAG
HHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEC
ETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALR
CCHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHH
IKEAVLAGSNFVVADMLEAASALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALF
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
ENDQEKALAEAVETLILSGPASQQLKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILS
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHH
QLTKKAAKFACFNQINTK
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAIQTFSTPRRLAVRVTGL
CCCCEEECCHHHCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEECC
ADKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKE
CCCHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHCCCCCEEECCHHHCCCCCCEEEEEHH
EIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVHTLTVLLDEQEFDLDFLDIKG
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHEEEEECCCCCCCEEEECCC
SRVSRGHRFLGQETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKEIEAKHGVRIEID
CHHHCCHHHCCCHHHHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHHHHHHHCCCEEEEC
ADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISV
HHHHHHHHHHHHCCHHHHCCCCCHHHHCCHHHHHHHHHHCCEEEEEECCCCCCCCHHHHC
RNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREH
CCCCHHHHHHHHCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
MIRTGQITVLLAEKASLSVDETVDLARAAAIYKFDLLTGMVGEFDELQGIMGEKYTLLAG
HHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEC
ETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALR
CCHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHH
IKEAVLAGSNFVVADMLEAASALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALF
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
ENDQEKALAEAVETLILSGPASQQLKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILS
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHH
QLTKKAAKFACFNQINTK
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA