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The map label for this gene is leuB [H]

Identifier: 221231948

GI number: 221231948

Start: 1126438

End: 1127475

Strand: Reverse

Name: leuB [H]

Synonym: SPN23F_11520

Alternate gene names: 221231948

Gene position: 1127475-1126438 (Counterclockwise)

Preceding gene: 221231949

Following gene: 221231947

Centisome position: 50.76

GC content: 43.64

Gene sequence:

>1038_bases
ATGGCAAAGAAAATAGTAGCTCTAGTAGGAGACGGAATTGGCCCAGAAATCATGGAGGCTGGTTTAGAAGTTCTGGAGGC
TCTAGCTGAAAAAACAGGTTTTGACTATGAGATTGACAGACGACCGTTCGGAGGTGCAGATATTGATGCAGCAGGACCTC
CCTTACCTGATGAAACCCTTAAGGCAAGTAGGGAAGCAGATGCTATCCTACTAGCAGCTATCGGTAGTCCTCAGTATGAT
GGAGCAGCGGTTCGCCCTGAACAAGGCCTGATGGCTCTCCGTAAGGAACTCAATCTTTACGCTAATATTCGTCCTGTAAA
AATCTTTGACAGTCTCAAGTATTTGTCACCACTCAAACCGGAACGAATTTCTGGTGTAGACTTTGTCGTGGTGCGTGAAT
TGACAGGCGAGATTTACTTTGGAGATCATATTCTTGAAGAGCGCAAAGCGCGTGATATCAACGACTATAGCTATGAGGAA
GTGGAGCGGATTATTCGCAAAGCCTTTGAAATTGCAAGAAATCGCAGAAAAATCGTTACTAGTATCGATAAGCAAAATGT
TCTAGCGACCTCAAAACTCTGGCGGAAAGTAGCTGAGGAAGTCGCACAGGATTTCCCAGATGTAACCTTGGAACACCAGC
TGGTAGACTCAGCTGCTATGCTTATGATTACCAATCCTGCTAAGTTTGATGTTATTGTAACGGAGAATCTTTTTGGAGAT
ATTTTATCTGATGAATCAAGCGTCTTATCTGGTACACTTGAGGTTATGCCATCAGCCAGTCATTCTGAAAATGGACCAAG
TCTCTATGAACCTATTCACGGTTCAGCACCTGATATTGCAGGTCAAGGAATTGCCAATCCTACTTCCATGATTTTATCAG
TTGCCATGATGTTGAGAGATAGTTTCGGACGTTATGAGGATGCAGAGCGTATCAAACATGCTGTTGAGACAAGTCTGGCG
GCAGGAATTTTAACGAGAGATATAGGAGGTCAGGCTTCAACAAAGGAAATGACGGAAGCTATTATTGCAAGGTTATGA

Upstream 100 bases:

>100_bases
CCTATATAAACGCTAATACCTTTGTTCAAAAAGAGAATGCAGGTGAGATGGGACGCAGTGTTTCTTACCACGATATGCCT
AGTGTGTAAAGGAGAAGGCT

Downstream 100 bases:

>100_bases
AGTTAGACGAAAAAATTACTCTAGTCCTTTTGATTTGGAATGTCATCATTTTCTTGATTTATGGCATTGACAAATTCAAG
GCAAGAAGAAGAACTTGGCG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETLKASREADAILLAAIGSPQYD
GAAVRPEQGLMALRKELNLYANIRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGD
ILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKHAVETSLA
AGILTRDIGGQASTKEMTEAIIARL

Sequences:

>Translated_345_residues
MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETLKASREADAILLAAIGSPQYD
GAAVRPEQGLMALRKELNLYANIRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGD
ILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKHAVETSLA
AGILTRDIGGQASTKEMTEAIIARL
>Mature_344_residues
AKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETLKASREADAILLAAIGSPQYDG
AAVRPEQGLMALRKELNLYANIRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEEV
ERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGDI
LSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKHAVETSLAA
GILTRDIGGQASTKEMTEAIIARL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=347, Percent_Identity=34.0057636887608, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI28178816, Length=349, Percent_Identity=28.9398280802292, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI28178821, Length=349, Percent_Identity=28.080229226361, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4758582, Length=257, Percent_Identity=29.9610894941634, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI28178819, Length=186, Percent_Identity=30.1075268817204, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI28178838, Length=230, Percent_Identity=29.5652173913043, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI87081683, Length=352, Percent_Identity=45.1704545454545, Blast_Score=284, Evalue=6e-78,
Organism=Escherichia coli, GI1788101, Length=354, Percent_Identity=35.8757062146893, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1787381, Length=373, Percent_Identity=29.2225201072386, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71986051, Length=348, Percent_Identity=33.6206896551724, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17505779, Length=349, Percent_Identity=30.6590257879656, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=355, Percent_Identity=29.0140845070423, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI25144293, Length=359, Percent_Identity=26.7409470752089, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=352, Percent_Identity=46.5909090909091, Blast_Score=297, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6322097, Length=358, Percent_Identity=37.4301675977654, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324709, Length=347, Percent_Identity=35.7348703170029, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324291, Length=263, Percent_Identity=36.8821292775665, Blast_Score=133, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24643270, Length=350, Percent_Identity=35.1428571428571, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24643268, Length=350, Percent_Identity=35.1428571428571, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24661184, Length=354, Percent_Identity=31.0734463276836, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI161078637, Length=354, Percent_Identity=30.5084745762712, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078635, Length=354, Percent_Identity=30.5084745762712, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078633, Length=354, Percent_Identity=30.5084745762712, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24650122, Length=354, Percent_Identity=30.5084745762712, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078639, Length=351, Percent_Identity=30.7692307692308, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI281362242, Length=346, Percent_Identity=29.4797687861272, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24648872, Length=346, Percent_Identity=29.4797687861272, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI20130355, Length=347, Percent_Identity=24.207492795389, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 37756; Mature: 37624

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00230 MAP1B_NEURAXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETL
CCCCEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCHHHH
KASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYANIRPVKIFDSLKYLSPLKP
HHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
ERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEEVERIIRKAFEIARNRRKIVT
CCCCCCCEEEEEHHCCCEEECHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCCEEEEEEEHHHHHH
ILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPTSMILSVAMMLRD
HHCCCHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SFGRYEDAERIKHAVETSLAAGILTRDIGGQASTKEMTEAIIARL
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFGGADIDAAGPPLPDETL
CCCEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCHHHH
KASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYANIRPVKIFDSLKYLSPLKP
HHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
ERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEEVERIIRKAFEIARNRRKIVT
CCCCCCCEEEEEHHCCCEEECHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSAAMLMITNPAKFDVIVTENLFGD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCCEEEEEEEHHHHHH
ILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSAPDIAGQGIANPTSMILSVAMMLRD
HHCCCHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SFGRYEDAERIKHAVETSLAAGILTRDIGGQASTKEMTEAIIARL
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11544234 [H]