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The map label for this gene is acoL [H]

Identifier: 221231874

GI number: 221231874

Start: 1041703

End: 1043388

Strand: Reverse

Name: acoL [H]

Synonym: SPN23F_10650

Alternate gene names: 221231874

Gene position: 1043388-1041703 (Counterclockwise)

Preceding gene: 221231875

Following gene: 221231873

Centisome position: 46.97

GC content: 42.88

Gene sequence:

>1686_bases
ATGCCAAAAGCCGGCGTGGATATGACAGAAGGACAAATCGTCCAATGGAATAAAAAAGTCGGAGAATTTGTAAAAGAAGG
AGAAATCCTTTTGGAAATCATGACTGATAAAGTCAGCATGGAATTGGAAGCCGAAGAAGATGGGTACTTGATTGCCATTC
TCAAAGGAGATGGTGAAACTGTCCCTGTAACGGAAGTTATCGGTTACCTTGGTGAAGAAAGGGAAAACATCCCAACAGCT
GGAGCAGCATCGCCAGAAGCTAGCCCTGTACCTGTAGCAAGTACCTCAAACGATGATGGTAAGAGCGATGATGCTTTTGA
TATCGTTGTGATTGGTGGAGGTCCTGCTGGTTATGTTGCAGCCATTAAAGCTGCCCAACTCGGCGGTAAGGTTGCCCTTG
TTGAGAAATCTGAACTTGGTGGAACCTGCTTAAACCGTGGATGTATTCCAACCAAGACCTACCTTCATAGCGCTGAAATT
ATTGAAAATATCGGTCATGCTGCAAATCGTGGTATCGTGATTGAAAATCCTAACTTCACTGTTGATATGGAAAAACTTTT
AGAAACTAAATCTAAAGTTGTTAATACTCTTGTTGGTGGTGTTGCAGGACTTCTTCGTAGCTACGGAGTTGCTGTTCATA
AAGGAATTGGTACAATCACTAAAGACAAGAACGTCTTGGTAAATGGTTCTGAATTGCTTGAAACCAAGAAAATCATTCTT
GCTGGTGGTTCAAAAGTCAGCAAGATCAACGTCCCTGGTATGGAATCTCCACTTGTCATGACTAGTGATGACATTCTTGA
AATGAACGAAGTGCCAGAAAGCCTTGTTATCATCGGTGGTGGAGTTGTCGGTATCGAACTCGGTCAGGCCTTCATGACAT
TTGGTTCAAAAGTGACTGTTATCGAAATGATGGACCGTATAGTTCCAGCTATGGATGTGGAAGTTTCTAAGAACCTTCGC
TTGATCCTTGAGCGTAAAGGAATGACCATCTTGACTGGTACTAAACTGCAAGAAATCATTGAGGAAAATGGTCAACTTCG
TATCAAGGTTGAAGGAAAAGACGATATCATCGCAAGCAAAGCTCTTCTTTCAATTGGTCGTATGCCAGACCTTGAAGGTA
TTGGAGAGGTTGAGTTTGAATTGGATCGTGGTTGTATCAAGGTCAACGAATACATGGAAACTTCAGTTCCAGGCATTTAT
GCACCAGGTGACATCAACGGTACTAAGATGTTGGCTCACGCAGCTTTCCGCATGGGTGAAGTTTCCGCTGAAAATGCCCT
TAAAGGAAATCATGCAGTTGCCAAATTGAATTTGACTCCTGCAGCCATCTACACTCTTCCTGAAGTAGCAGCAGTAGGTT
TGACAGAAGAACAAGCCCGTGAGAAATACGATGTTGCCATCGGTAAGTTTAACTTTGCTGCTAACGGTCGTGCTATTGCA
TCTGACGCAGCTCAAGGTTTCGTAAAAGTTATCGCTGATAAGAAATACGGAGAAATCCTTGGTGTACACATTATTGGTCC
TGCAGCCGCAGAATTAATAAACGAAGCATCAAGCATCATCGAAATGGAAATCACTGTTGAGGAAATGCTGAAGACCATCC
ACGGGCACCCAACTTACTCTGAAGTGATGTACGAAGCGTTTGCAGATGTTCTAGGAATGGCCATCCATTCACCTAAGAAA
AAATAA

Upstream 100 bases:

>100_bases
AGAGTTGATTGAAACCCCAATCTCAATGTTGATTTAAGAGCAAGAGTATTCATTTTTAAGATATAGATAAAGGAAGGAAG
ACATGGCCTTAGAAGTAATT

Downstream 100 bases:

>100_bases
AACGAAGATAGATTAGACTGGAAGGTTATTTCCAGTTTCTATCATATAAAGTAAAGGGATATCATGAAATACATTATCAA
TCATTCAAATGACACTGCTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase [H]

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTA
GAASPEASPVPVASTSNDDGKSDDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGTITKDKNVLVNGSELLETKKIIL
AGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLR
LILERKGMTILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMETSVPGIY
APGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIA
SDAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKK
K

Sequences:

>Translated_561_residues
MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTA
GAASPEASPVPVASTSNDDGKSDDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGTITKDKNVLVNGSELLETKKIIL
AGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLR
LILERKGMTILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMETSVPGIY
APGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIA
SDAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKK
K
>Mature_560_residues
PKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAG
AASPEASPVPVASTSNDDGKSDDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEII
ENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGTITKDKNVLVNGSELLETKKIILA
GGSKVSKINVPGMESPLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRL
ILERKGMTILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMETSVPGIYA
PGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIAS
DAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK

Specific function: Has chromate reductase activity [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=38.5106382978723, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI50301238, Length=457, Percent_Identity=26.9146608315098, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI22035672, Length=460, Percent_Identity=29.3478260869565, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI33519430, Length=457, Percent_Identity=24.945295404814, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI33519428, Length=457, Percent_Identity=24.945295404814, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI33519426, Length=457, Percent_Identity=24.945295404814, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI148277065, Length=457, Percent_Identity=24.945295404814, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI148277071, Length=457, Percent_Identity=24.945295404814, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI291045266, Length=461, Percent_Identity=24.9457700650759, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI291045268, Length=452, Percent_Identity=24.3362831858407, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1786307, Length=448, Percent_Identity=38.3928571428571, Blast_Score=279, Evalue=4e-76,
Organism=Escherichia coli, GI87082354, Length=459, Percent_Identity=27.8867102396514, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI87081717, Length=460, Percent_Identity=28.0434782608696, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789915, Length=430, Percent_Identity=27.6744186046512, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1789065, Length=297, Percent_Identity=24.5791245791246, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=473, Percent_Identity=39.5348837209302, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17557007, Length=487, Percent_Identity=27.3100616016427, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=450, Percent_Identity=27.3333333333333, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=450, Percent_Identity=27.3333333333333, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=478, Percent_Identity=24.4769874476987, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=484, Percent_Identity=38.0165289256198, Blast_Score=263, Evalue=8e-71,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=28.9029535864979, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6325166, Length=463, Percent_Identity=28.0777537796976, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324258, Length=79, Percent_Identity=39.2405063291139, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=41.8300653594771, Blast_Score=295, Evalue=8e-80,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=26.3713080168776, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24640551, Length=501, Percent_Identity=24.9500998003992, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24640553, Length=479, Percent_Identity=24.6346555323591, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24640549, Length=465, Percent_Identity=24.5161290322581, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 59669; Mature: 59537

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAILKGDGET
CCCCCCCCCCCEEEEEHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCEEEEEEECCCCE
VPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKSDDAFDIVVIGGGPAGYVA
ECHHHHHHHHCCHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCCHHH
AIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEIIENIGHAANRGIVIENPNFT
HHHHHHCCCEEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHCHHHCCCEEEECCCCE
VDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGTITKDKNVLVNGSELLETKKIIL
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCEECCCCEEECCHHHHHEEEEEE
AGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTFGSKVTV
ECCCEEEEEECCCCCCCEEECCCCHHHHHCCCCEEEEEECCEEEEHHHHHHHHCCCCCHH
IEMMDRIVPAMDVEVSKNLRLILERKGMTILTGTKLQEIIEENGQLRIKVEGKDDIIASK
HHHHHHHCCCCCEEECCCEEEEEEECCCEEEECHHHHHHHHCCCEEEEEECCCCCHHHHH
ALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMETSVPGIYAPGDINGTKMLAHAAFRMGE
HHHHHCCCCCCCCCCCEEEEECCCEEEEHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCC
VSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIA
CCHHHCCCCCCEEEEEECCHHHHHCCCHHHHCCCCHHHHHHHHCEEEEEEEECCCCCEEH
SDAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYS
HHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHEEEEEEHHHHHHHHCCCCCHH
EVMYEAFADVLGMAIHSPKKK
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEAEEDGYLIAILKGDGET
CCCCCCCCCCEEEEEHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCEEEEEEECCCCE
VPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKSDDAFDIVVIGGGPAGYVA
ECHHHHHHHHCCHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCCHHH
AIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEIIENIGHAANRGIVIENPNFT
HHHHHHCCCEEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHCHHHCCCEEEECCCCE
VDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGTITKDKNVLVNGSELLETKKIIL
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCEECCCCEEECCHHHHHEEEEEE
AGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLVIIGGGVVGIELGQAFMTFGSKVTV
ECCCEEEEEECCCCCCCEEECCCCHHHHHCCCCEEEEEECCEEEEHHHHHHHHCCCCCHH
IEMMDRIVPAMDVEVSKNLRLILERKGMTILTGTKLQEIIEENGQLRIKVEGKDDIIASK
HHHHHHHCCCCCEEECCCEEEEEEECCCEEEECHHHHHHHHCCCEEEEEECCCCCHHHHH
ALLSIGRMPDLEGIGEVEFELDRGCIKVNEYMETSVPGIYAPGDINGTKMLAHAAFRMGE
HHHHHCCCCCCCCCCCEEEEECCCEEEEHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCC
VSAENALKGNHAVAKLNLTPAAIYTLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIA
CCHHHCCCCCCEEEEEECCHHHHHCCCHHHHCCCCHHHHHHHHCEEEEEEEECCCCCEEH
SDAAQGFVKVIADKKYGEILGVHIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYS
HHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHEEEEEEHHHHHHHHCCCCCHH
EVMYEAFADVLGMAIHSPKKK
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA