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The map label for this gene is ytwF [H]

Identifier: 221231017

GI number: 221231017

Start: 83311

End: 83607

Strand: Reverse

Name: ytwF [H]

Synonym: SPN23F_00850

Alternate gene names: 221231017

Gene position: 83607-83311 (Counterclockwise)

Preceding gene: 221231031

Following gene: 221231016

Centisome position: 3.76

GC content: 43.77

Gene sequence:

>297_bases
ATGGAAACTAGTATCAGCATGGCTGACTTTTATGGAAAATACCAAAATGAAAATCTAGAACTTATCGATGTCCGTGAAGC
ACATGAATTCCAAGCAGGACATGCACCAGGTGCCAAAAATCTTCCATTAAGTACCTTAGAGCAAGGCTACAAAGAACTCA
AACCTGACCATGAATACTATGTCATTTGTCAAGGAGGAGTCCGCTCTGCATCTACCTGCCAGTTTCTCAGCTCCCAAGGC
CTCACCGTTACCAACGTAGAAGGTGGTATGAATGCTTGGCCCGGTCAAGTAGAATAA

Upstream 100 bases:

>100_bases
TATATCGCAGAGCCTATCCTCAAGCAAGCAGGATTTACCGCCCAAAACCTTGACGGCGCTTATTCACTATACAAAATGGT
TAACCCAGAAGGAGTAGAAT

Downstream 100 bases:

>100_bases
TCAAGAGGAGTTGGGCAAAAACTCAATTTCAGGAAAAGATGAAGTAATACTCAATGAAAATCAAAGTGCAAACTAGGAAG
TTAGCCGCAGGCTGCTCAAA

Product: hypothetical protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 98; Mature: 98

Protein sequence:

>98_residues
METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLSTLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQG
LTVTNVEGGMNAWPGQVE

Sequences:

>Translated_98_residues
METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLSTLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQG
LTVTNVEGGMNAWPGQVE
>Mature_98_residues
METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLSTLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQG
LTVTNVEGGMNAWPGQVE

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 10813; Mature: 10813

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLSTLEQGYKELKPDHEYY
CCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEE
VICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE
EEECCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLSTLEQGYKELKPDHEYY
CCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEE
VICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE
EEECCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]