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The map label for this gene is purD [H]

Identifier: 220933141

GI number: 220933141

Start: 2521541

End: 2522818

Strand: Reverse

Name: purD [H]

Synonym: Hore_23090

Alternate gene names: 220933141

Gene position: 2522818-2521541 (Counterclockwise)

Preceding gene: 220933142

Following gene: 220933140

Centisome position: 97.85

GC content: 46.48

Gene sequence:

>1278_bases
ATGAAAATTTTAGTTGTAGGAAGTGGGGGTAGGGAGCATGCCCTGGTCTGGAAGTTATACCAGAGTGAACGTGTTGAGCG
GATATTTGTGGCCCCCGGTAATGACGGCATGGCCGAGCTGGCTGAGATTGTCAACATTAAAAGTAATGATATTAAGGGAC
TGGCTGACTGGGCTGAAGAAAACGGTATTGATCTTACGGTAGTCGGTCCTGAAGAACCCCTGGTTAACGGAATTGTCGAT
GAGTTTGAGTCCCGGGGCCTCCGTATTTTTGGGCCTGATAAAAAGGCAGCCCGGATTGAGGGGAGTAAGGTCTTTGCTAA
AAGTATTTTAAAGAAATACAATATCCCTACTGCTGAATTTGAGGTATTTACTACCTTTGAGGAGGCCCTGGCCTATTTAA
AACAGGCCGAATATCCCCTGGTAATTAAGGCTGAAGGGCTGGCTGCCGGGAAAGGTGTTTTTATTGCCACTGACAAAGAA
AAGGCCTGTCAGGCTGTGGAGAAAATCATGGAAAAAAGGATTTTCGGTGATGCTGGTCAGAGGATAGTGGTTGAAGATTT
TCTGGAAGGTGAGGAAGTCTCGGTACTGGCCCTGACTGACGGTAAGACCATAATACCCATGGTACCGGCCCAGGACCATA
AACCGGTTTTTGACGGTGATGAGGGCCCCAATACCGGGGGGATGGGGGCGTATTCCCCGGTTCCCTTTGTCGGGTCTGAC
CTCCAGCACGAGGTATATGAAAACATCCTGGAACCGACCGTCAGGGCCCTGAATAAAGAGGGTATAAAATATAAGGGAAT
TCTCTATGCCGGATTGATTTTGACAGATTCAGGCCCCAAAGTGCTGGAGTTTAATGCCCGCTTTGGCGACCCCGAGACCC
AGGTTATAATACCCAGGCTTAAAACAGATCTTGTTGATTTAATTGATGCTGTTATTGATGAAAAACTTGAAGGTAAAAGG
CTTGACTGGGATGACAGGGCAGCCGTGTGTGTGGTTCTGGCATCAGGGGGTTATCCCTTTGATTATGATACCGGATACCG
TATCAAAGGACTGGAACAGCTGGCCCAGTATGATAATTTCCTTATCTTTCATGCCGGGACTAAAATGGAAGACGGCCATT
TTGTAACCGATGGTGGCCGGGTTATGGGAATAACCGTGCTTGGTAATGGATTAATAGATGCTATAAATCAGGTATATCAG
GCTGTAGAGGATATATATTTTGAGGATATGCATTACCGGACCGATATTGGCTACAAGGCTATTGTTGATGATTATTAA

Upstream 100 bases:

>100_bases
ATGTGAAGTTAATAGAGGATATGGTTAAGGTTCCGGTAACCATTATCGGGGTTGGTCCTGGTCGAAAAGAAGCGATAAGG
AGAGAGAAGGAGGTTAATTA

Downstream 100 bases:

>100_bases
GCATATATAACTCCTGGTGGATGACTAAAACTAATTATAAGGAACCCACAAATCTATAAGTGAGTACAAAAAGTGAGTTC
AAAAAAGTATATTTTTGAAG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKILVVGSGGREHALVWKLYQSERVERIFVAPGNDGMAELAEIVNIKSNDIKGLADWAEENGIDLTVVGPEEPLVNGIVD
EFESRGLRIFGPDKKAARIEGSKVFAKSILKKYNIPTAEFEVFTTFEEALAYLKQAEYPLVIKAEGLAAGKGVFIATDKE
KACQAVEKIMEKRIFGDAGQRIVVEDFLEGEEVSVLALTDGKTIIPMVPAQDHKPVFDGDEGPNTGGMGAYSPVPFVGSD
LQHEVYENILEPTVRALNKEGIKYKGILYAGLILTDSGPKVLEFNARFGDPETQVIIPRLKTDLVDLIDAVIDEKLEGKR
LDWDDRAAVCVVLASGGYPFDYDTGYRIKGLEQLAQYDNFLIFHAGTKMEDGHFVTDGGRVMGITVLGNGLIDAINQVYQ
AVEDIYFEDMHYRTDIGYKAIVDDY

Sequences:

>Translated_425_residues
MKILVVGSGGREHALVWKLYQSERVERIFVAPGNDGMAELAEIVNIKSNDIKGLADWAEENGIDLTVVGPEEPLVNGIVD
EFESRGLRIFGPDKKAARIEGSKVFAKSILKKYNIPTAEFEVFTTFEEALAYLKQAEYPLVIKAEGLAAGKGVFIATDKE
KACQAVEKIMEKRIFGDAGQRIVVEDFLEGEEVSVLALTDGKTIIPMVPAQDHKPVFDGDEGPNTGGMGAYSPVPFVGSD
LQHEVYENILEPTVRALNKEGIKYKGILYAGLILTDSGPKVLEFNARFGDPETQVIIPRLKTDLVDLIDAVIDEKLEGKR
LDWDDRAAVCVVLASGGYPFDYDTGYRIKGLEQLAQYDNFLIFHAGTKMEDGHFVTDGGRVMGITVLGNGLIDAINQVYQ
AVEDIYFEDMHYRTDIGYKAIVDDY
>Mature_425_residues
MKILVVGSGGREHALVWKLYQSERVERIFVAPGNDGMAELAEIVNIKSNDIKGLADWAEENGIDLTVVGPEEPLVNGIVD
EFESRGLRIFGPDKKAARIEGSKVFAKSILKKYNIPTAEFEVFTTFEEALAYLKQAEYPLVIKAEGLAAGKGVFIATDKE
KACQAVEKIMEKRIFGDAGQRIVVEDFLEGEEVSVLALTDGKTIIPMVPAQDHKPVFDGDEGPNTGGMGAYSPVPFVGSD
LQHEVYENILEPTVRALNKEGIKYKGILYAGLILTDSGPKVLEFNARFGDPETQVIIPRLKTDLVDLIDAVIDEKLEGKR
LDWDDRAAVCVVLASGGYPFDYDTGYRIKGLEQLAQYDNFLIFHAGTKMEDGHFVTDGGRVMGITVLGNGLIDAINQVYQ
AVEDIYFEDMHYRTDIGYKAIVDDY

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=49.2924528301887, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=49.2924528301887, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=49.2924528301887, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=49.2924528301887, Blast_Score=423, Evalue=1e-118,
Organism=Escherichia coli, GI1790438, Length=425, Percent_Identity=52, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=40.661938534279, Blast_Score=313, Evalue=2e-85,
Organism=Saccharomyces cerevisiae, GI6321203, Length=439, Percent_Identity=42.8246013667426, Blast_Score=340, Evalue=2e-94,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=46.8531468531469, Blast_Score=354, Evalue=5e-98,
Organism=Drosophila melanogaster, GI24582400, Length=429, Percent_Identity=46.8531468531469, Blast_Score=353, Evalue=1e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46885; Mature: 46885

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVVGSGGREHALVWKLYQSERVERIFVAPGNDGMAELAEIVNIKSNDIKGLADWAEE
CEEEEEECCCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHC
NGIDLTVVGPEEPLVNGIVDEFESRGLRIFGPDKKAARIEGSKVFAKSILKKYNIPTAEF
CCCEEEEECCCCHHHHHHHHHHHHCCEEEECCCCCCEEECCHHHHHHHHHHHCCCCCCCE
EVFTTFEEALAYLKQAEYPLVIKAEGLAAGKGVFIATDKEKACQAVEKIMEKRIFGDAGQ
EEEHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCC
RIVVEDFLEGEEVSVLALTDGKTIIPMVPAQDHKPVFDGDEGPNTGGMGAYSPVPFVGSD
EEEEHHHCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LQHEVYENILEPTVRALNKEGIKYKGILYAGLILTDSGPKVLEFNARFGDPETQVIIPRL
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCEEEEECCCCCCCCEEEECHH
KTDLVDLIDAVIDEKLEGKRLDWDDRAAVCVVLASGGYPFDYDTGYRIKGLEQLAQYDNF
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEHHHHHHHHHCCE
LIFHAGTKMEDGHFVTDGGRVMGITVLGNGLIDAINQVYQAVEDIYFEDMHYRTDIGYKA
EEEECCCCCCCCEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCEEECCCCCEE
IVDDY
ECCCC
>Mature Secondary Structure
MKILVVGSGGREHALVWKLYQSERVERIFVAPGNDGMAELAEIVNIKSNDIKGLADWAEE
CEEEEEECCCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHC
NGIDLTVVGPEEPLVNGIVDEFESRGLRIFGPDKKAARIEGSKVFAKSILKKYNIPTAEF
CCCEEEEECCCCHHHHHHHHHHHHCCEEEECCCCCCEEECCHHHHHHHHHHHCCCCCCCE
EVFTTFEEALAYLKQAEYPLVIKAEGLAAGKGVFIATDKEKACQAVEKIMEKRIFGDAGQ
EEEHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCC
RIVVEDFLEGEEVSVLALTDGKTIIPMVPAQDHKPVFDGDEGPNTGGMGAYSPVPFVGSD
EEEEHHHCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LQHEVYENILEPTVRALNKEGIKYKGILYAGLILTDSGPKVLEFNARFGDPETQVIIPRL
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCEEEEECCCCCCCCEEEECHH
KTDLVDLIDAVIDEKLEGKRLDWDDRAAVCVVLASGGYPFDYDTGYRIKGLEQLAQYDNF
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEHHHHHHHHHCCE
LIFHAGTKMEDGHFVTDGGRVMGITVLGNGLIDAINQVYQAVEDIYFEDMHYRTDIGYKA
EEEECCCCCCCCEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCEEECCCCCEE
IVDDY
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]