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The map label for this gene is purR [H]

Identifier: 220933134

GI number: 220933134

Start: 2514652

End: 2515662

Strand: Reverse

Name: purR [H]

Synonym: Hore_23020

Alternate gene names: 220933134

Gene position: 2515662-2514652 (Counterclockwise)

Preceding gene: 220933139

Following gene: 220933133

Centisome position: 97.58

GC content: 36.99

Gene sequence:

>1011_bases
ATGGCTGTGACTATAAAAGATATTGCCAGGGCTGCTGGTGTGTCAAAGACTACTGTTTCCAGGGCTTTAAATAATAAGGG
TCGAATTAGTCCGGAAACCAGAGAAAAAATCCTTAAAATCGCTGCAAAAATGAATTACCGTCATAATAAAATAGCTACCA
GTTTACGGTCCAATAAATCCATGGTAATTGGTCTAATTTTCCCTGGATTTATGGCAGGCCATTTTTATAGTGAAATTTTT
CATGGCATTGAGGCTTACTGTACTGAAAAAGGCTTTGGGGTCTTAATTGGTTGTTCTGATGGATTAGCTCAAAAAGAGGA
AGAGATAATTAAATTACTTCAGGAACGCAGGGTTGATGGTATTATAATTGCCCCTACCCATGGGGTTGATCTGGATTATT
ATCATCAGTTTAAAAAGGAAAAACTGCCTTTTATCTTTATAGATAAATACATACCGGGTATTGAAGCAGATAGAATAGTT
GTTGATAATAAAAAAGGTGCCTATCTTGCCGTAACTCACCTTATCGAGAGAGGTCATAAAAAGATTGCTTTATTAAGTGG
ACCGGAATACCCCTGTACTACAATTGAAGGAAGATTAGAAGGATATCTTAAGGCTCTTGAAGATAATGGTCTTACATATA
GGAAAATCATAAAAACAGATAAAAATGTTTATAACCAGAGAGAAAGTGGTTATAAGGCAACAAAGGAATTTATAAAAGAT
AATGATGGTGTAACCGCCATCTTTGCTATTAACGATAGTGTCGCTATAGGTGCCATGAGGGCAATCAGGGAGGCTGGACT
TCGAGTCCCGCAGGATATGGCCATTGTTGGCTTTAATGATGATGATATTTCTTTATATATTGAAAATTCACTTACAACTA
TATCTGTTCCCAAATATAAATTAGGGGAAAAGGCCGCTCAACTGATATTAGAACGTATTGAGGGGCAGGCCGATACAGGA
CAGAGGATTATTACCCTGGAACCTGATTTAGTGGTTAGAGATACAACTTAA

Upstream 100 bases:

>100_bases
AAGGAATTTTTATTTTTGTCTAGAAATAATATAAGCGTAGAGTAAAGAGCTATATATTTTTTTAATTGCGGGAACGATAA
CGCATAAAGGATGATTGGTT

Downstream 100 bases:

>100_bases
TTTTTTTCCATAATGCGGGAACGATAACGCAAATTGAATATTTTTTGAAAATATAGATGATTGATTAGTGCCATAAAATT
GATTGAATTTAGCCAATAAA

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MAVTIKDIARAAGVSKTTVSRALNNKGRISPETREKILKIAAKMNYRHNKIATSLRSNKSMVIGLIFPGFMAGHFYSEIF
HGIEAYCTEKGFGVLIGCSDGLAQKEEEIIKLLQERRVDGIIIAPTHGVDLDYYHQFKKEKLPFIFIDKYIPGIEADRIV
VDNKKGAYLAVTHLIERGHKKIALLSGPEYPCTTIEGRLEGYLKALEDNGLTYRKIIKTDKNVYNQRESGYKATKEFIKD
NDGVTAIFAINDSVAIGAMRAIREAGLRVPQDMAIVGFNDDDISLYIENSLTTISVPKYKLGEKAAQLILERIEGQADTG
QRIITLEPDLVVRDTT

Sequences:

>Translated_336_residues
MAVTIKDIARAAGVSKTTVSRALNNKGRISPETREKILKIAAKMNYRHNKIATSLRSNKSMVIGLIFPGFMAGHFYSEIF
HGIEAYCTEKGFGVLIGCSDGLAQKEEEIIKLLQERRVDGIIIAPTHGVDLDYYHQFKKEKLPFIFIDKYIPGIEADRIV
VDNKKGAYLAVTHLIERGHKKIALLSGPEYPCTTIEGRLEGYLKALEDNGLTYRKIIKTDKNVYNQRESGYKATKEFIKD
NDGVTAIFAINDSVAIGAMRAIREAGLRVPQDMAIVGFNDDDISLYIENSLTTISVPKYKLGEKAAQLILERIEGQADTG
QRIITLEPDLVVRDTT
>Mature_335_residues
AVTIKDIARAAGVSKTTVSRALNNKGRISPETREKILKIAAKMNYRHNKIATSLRSNKSMVIGLIFPGFMAGHFYSEIFH
GIEAYCTEKGFGVLIGCSDGLAQKEEEIIKLLQERRVDGIIIAPTHGVDLDYYHQFKKEKLPFIFIDKYIPGIEADRIVV
DNKKGAYLAVTHLIERGHKKIALLSGPEYPCTTIEGRLEGYLKALEDNGLTYRKIIKTDKNVYNQRESGYKATKEFIKDN
DGVTAIFAINDSVAIGAMRAIREAGLRVPQDMAIVGFNDDDISLYIENSLTTISVPKYKLGEKAAQLILERIEGQADTGQ
RIITLEPDLVVRDTT

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=338, Percent_Identity=32.2485207100592, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=32.258064516129, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=30.6547619047619, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1787580, Length=322, Percent_Identity=31.9875776397516, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1788474, Length=319, Percent_Identity=30.0940438871473, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1786540, Length=341, Percent_Identity=32.8445747800587, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1789068, Length=305, Percent_Identity=30.4918032786885, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=30.8474576271186, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1787906, Length=324, Percent_Identity=29.6296296296296, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790715, Length=324, Percent_Identity=27.4691358024691, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI48994940, Length=329, Percent_Identity=27.355623100304, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1786268, Length=314, Percent_Identity=23.2484076433121, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789456, Length=319, Percent_Identity=26.6457680250784, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790689, Length=322, Percent_Identity=22.9813664596273, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37455; Mature: 37324

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTIKDIARAAGVSKTTVSRALNNKGRISPETREKILKIAAKMNYRHNKIATSLRSNKS
CEEEHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
MVIGLIFPGFMAGHFYSEIFHGIEAYCTEKGFGVLIGCSDGLAQKEEEIIKLLQERRVDG
EEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCC
IIIAPTHGVDLDYYHQFKKEKLPFIFIDKYIPGIEADRIVVDNKKGAYLAVTHLIERGHK
EEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHCCCC
KIALLSGPEYPCTTIEGRLEGYLKALEDNGLTYRKIIKTDKNVYNQRESGYKATKEFIKD
EEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHCC
NDGVTAIFAINDSVAIGAMRAIREAGLRVPQDMAIVGFNDDDISLYIENSLTTISVPKYK
CCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCEEEEECCCHH
LGEKAAQLILERIEGQADTGQRIITLEPDLVVRDTT
HHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCC
>Mature Secondary Structure 
AVTIKDIARAAGVSKTTVSRALNNKGRISPETREKILKIAAKMNYRHNKIATSLRSNKS
EEEHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
MVIGLIFPGFMAGHFYSEIFHGIEAYCTEKGFGVLIGCSDGLAQKEEEIIKLLQERRVDG
EEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCC
IIIAPTHGVDLDYYHQFKKEKLPFIFIDKYIPGIEADRIVVDNKKGAYLAVTHLIERGHK
EEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHCCCC
KIALLSGPEYPCTTIEGRLEGYLKALEDNGLTYRKIIKTDKNVYNQRESGYKATKEFIKD
EEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHCC
NDGVTAIFAINDSVAIGAMRAIREAGLRVPQDMAIVGFNDDDISLYIENSLTTISVPKYK
CCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCEEEEECCCHH
LGEKAAQLILERIEGQADTGQRIITLEPDLVVRDTT
HHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA