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The map label for this gene is purH [H]

Identifier: 220933041

GI number: 220933041

Start: 2405175

End: 2406782

Strand: Reverse

Name: purH [H]

Synonym: Hore_22090

Alternate gene names: 220933041

Gene position: 2406782-2405175 (Counterclockwise)

Preceding gene: 220933042

Following gene: 220933034

Centisome position: 93.35

GC content: 45.27

Gene sequence:

>1608_bases
ATGGCTAAAAGAGCCTTATTGAGTGTTTCTGATAAGAGGGGAATAGTGAAAATGGCCCGGGGGCTTGTTGACCTGGGTTA
TGAAATAATATCCACCGGGGGTACGGCCCGTAAACTCCGTGAAGGTGGTCTGGAGGTTATTGATGTCAGCCAGATAACCC
GTTTTCCTGAAATGATGGATGGACGGGTTAAAACCCTTCATCCGGCTATTCACGGTGGTCTTCTGGCTTTAAGGGATAAC
TCCGGTCATATGGAACAGCTTGAAGAGCAGGGGATTAAACCCATTGATATGGTAGTGGTCAACCTTTACCCCTTTGCTGA
GACCATTAGCCGTCCGGGAGTAACCCTGGAGGAAGCCATTGAAAATATTGATATCGGAGGTCCCAGTATGATTAGATCAG
CTGCTAAAAACTATTGTAGTGTTGCTGTAGTGACCGATCCTGATGATTATGATAAAATTCTTACTGAACTTAATGAAAAT
GATGGAGACCTCTCTTTAAAAACAAGGCAAAGGCTGGCAGTTAAGGCTTTTAGAATGACAGCAGAGTATGATACAACTAT
TTTTGATTATCTACAGGGTAGAAATGAACGGGACAAAAAAGAGACCAGTAAGGCTAAAAGCAGTAAAGAAGAAGCAGATA
TTGTTTTCCCGGAAGTTATTAGCTTAAATTATATAAAGAAACAGGATTTGAGGTATGGGGAAAACCCTCATCAGAGGGCT
GTTTTTTACCGGGAAACAAATTTAAAAGAGCCCTCTATTACTACTGCTGAAAAGCTTCATGGCAAGGAGCTTTCCTTTAA
CAATATCAATGATGCTAACGGGGCCCTGGAACTGGTTAAGGAGTTTACCGACCGGCCAGCCGCTGCAGTTATTAAACATG
CCAATCCCTGTGGAATGGCAGTAGCCGATACCCTGGAAGAGGCTTACCGGAAGGCTCATGCCGGGGATCCCCTGTCAGCT
TTTGGTAGTATTGTGGCCTTAAACCGTGAAGTTTCACCAGAAGTAGCCCGGGAGATTGCCGGCAAAGATAAGTTTGTCGA
GGTTGTTATTGCCCCTGACTATATGACGGAGGCTCTCGAAATCCTTAAAAATAGATGGAAAAATGTCAGGCTTTTAAGGA
CAGGAGATCTTTTTATTAACCGGGAGAAGCCTGGCTATGACATGAAGAAAGTTACCGGTGGTTTACTTGTTCAGGAACGG
GACCTTGCTATTATAAATGAGGACGACCTTAAGATAGTTACTGAAGTGAAACCAACTGAAGGACAGTTAAGGGATTTGTT
GTTTGCCTGGAAGGTTGTAAAACATGTTAAGTCCAATGCAATCGTTATGGCTAAAGACGAGATGGTAGTAGGTGTCGGGG
CCGGGCAGATGAGCCGGGTGGATGCCATGATTATTGCCGGTCGTAAGTCAGAAGGAAGGCAGGAAGGCGGGGTTGTTGCT
TCAGATGCTTTCTTCCCCTTCCCGGATGCCATTGAGGAGGCGGCTGCCAGAGGGATTAAGGCTATCATCCAGCCCGGTGG
TTCAATCAGGGATGACCAGGTGATTGAAGCTGCCAATGACCATAAAATTGCCATGGTCTTTACCGGGAGAAGGCATTTCA
GGCATTAA

Upstream 100 bases:

>100_bases
GGAGTGCCTGTGGCACAACCATAATGTTCCTGTACGGTTTTTTTAAATACGGAGTGCCTGTGGCACAACCTTAAATATTA
AAAGGAGGTATATACTGGAT

Downstream 100 bases:

>100_bases
CTTCTAATAATTAGAAAATGTATTAAGCCAGTATATTTTAAAATCCTGAAAATTAGTTAGAACTATACAGTAAGTAAATC
TATAGTCAATTTATATTTAA

Product: IMP cyclohydrolase;phosphoribosylaminoimidazolecarboxamide formyltransferase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MAKRALLSVSDKRGIVKMARGLVDLGYEIISTGGTARKLREGGLEVIDVSQITRFPEMMDGRVKTLHPAIHGGLLALRDN
SGHMEQLEEQGIKPIDMVVVNLYPFAETISRPGVTLEEAIENIDIGGPSMIRSAAKNYCSVAVVTDPDDYDKILTELNEN
DGDLSLKTRQRLAVKAFRMTAEYDTTIFDYLQGRNERDKKETSKAKSSKEEADIVFPEVISLNYIKKQDLRYGENPHQRA
VFYRETNLKEPSITTAEKLHGKELSFNNINDANGALELVKEFTDRPAAAVIKHANPCGMAVADTLEEAYRKAHAGDPLSA
FGSIVALNREVSPEVAREIAGKDKFVEVVIAPDYMTEALEILKNRWKNVRLLRTGDLFINREKPGYDMKKVTGGLLVQER
DLAIINEDDLKIVTEVKPTEGQLRDLLFAWKVVKHVKSNAIVMAKDEMVVGVGAGQMSRVDAMIIAGRKSEGRQEGGVVA
SDAFFPFPDAIEEAAARGIKAIIQPGGSIRDDQVIEAANDHKIAMVFTGRRHFRH

Sequences:

>Translated_535_residues
MAKRALLSVSDKRGIVKMARGLVDLGYEIISTGGTARKLREGGLEVIDVSQITRFPEMMDGRVKTLHPAIHGGLLALRDN
SGHMEQLEEQGIKPIDMVVVNLYPFAETISRPGVTLEEAIENIDIGGPSMIRSAAKNYCSVAVVTDPDDYDKILTELNEN
DGDLSLKTRQRLAVKAFRMTAEYDTTIFDYLQGRNERDKKETSKAKSSKEEADIVFPEVISLNYIKKQDLRYGENPHQRA
VFYRETNLKEPSITTAEKLHGKELSFNNINDANGALELVKEFTDRPAAAVIKHANPCGMAVADTLEEAYRKAHAGDPLSA
FGSIVALNREVSPEVAREIAGKDKFVEVVIAPDYMTEALEILKNRWKNVRLLRTGDLFINREKPGYDMKKVTGGLLVQER
DLAIINEDDLKIVTEVKPTEGQLRDLLFAWKVVKHVKSNAIVMAKDEMVVGVGAGQMSRVDAMIIAGRKSEGRQEGGVVA
SDAFFPFPDAIEEAAARGIKAIIQPGGSIRDDQVIEAANDHKIAMVFTGRRHFRH
>Mature_534_residues
AKRALLSVSDKRGIVKMARGLVDLGYEIISTGGTARKLREGGLEVIDVSQITRFPEMMDGRVKTLHPAIHGGLLALRDNS
GHMEQLEEQGIKPIDMVVVNLYPFAETISRPGVTLEEAIENIDIGGPSMIRSAAKNYCSVAVVTDPDDYDKILTELNEND
GDLSLKTRQRLAVKAFRMTAEYDTTIFDYLQGRNERDKKETSKAKSSKEEADIVFPEVISLNYIKKQDLRYGENPHQRAV
FYRETNLKEPSITTAEKLHGKELSFNNINDANGALELVKEFTDRPAAAVIKHANPCGMAVADTLEEAYRKAHAGDPLSAF
GSIVALNREVSPEVAREIAGKDKFVEVVIAPDYMTEALEILKNRWKNVRLLRTGDLFINREKPGYDMKKVTGGLLVQERD
LAIINEDDLKIVTEVKPTEGQLRDLLFAWKVVKHVKSNAIVMAKDEMVVGVGAGQMSRVDAMIIAGRKSEGRQEGGVVAS
DAFFPFPDAIEEAAARGIKAIIQPGGSIRDDQVIEAANDHKIAMVFTGRRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=496, Percent_Identity=38.508064516129, Blast_Score=282, Evalue=6e-76,
Organism=Escherichia coli, GI1790439, Length=536, Percent_Identity=54.1044776119403, Blast_Score=563, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71985564, Length=528, Percent_Identity=33.7121212121212, Blast_Score=251, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI71985574, Length=355, Percent_Identity=26.4788732394366, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323056, Length=502, Percent_Identity=38.8446215139442, Blast_Score=293, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6323768, Length=531, Percent_Identity=37.0998116760829, Blast_Score=292, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24649832, Length=501, Percent_Identity=37.9241516966068, Blast_Score=283, Evalue=2e-76,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 59284; Mature: 59152

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRALLSVSDKRGIVKMARGLVDLGYEIISTGGTARKLREGGLEVIDVSQITRFPEMMD
CCCCCEEECCCCCCHHHHHHHHHHHCHHHHHCCCHHHHHHHCCCEEEEHHHHHHCHHHHC
GRVKTLHPAIHGGLLALRDNSGHMEQLEEQGIKPIDMVVVNLYPFAETISRPGVTLEEAI
CCHHHCCHHHHCCEEEEECCCCHHHHHHHCCCCEEHEEEEEHHHHHHHHCCCCCCHHHHH
ENIDIGGPSMIRSAAKNYCSVAVVTDPDDYDKILTELNENDGDLSLKTRQRLAVKAFRMT
HHCCCCCHHHHHHHHHCCEEEEEEECCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHH
AEYDTTIFDYLQGRNERDKKETSKAKSSKEEADIVFPEVISLNYIKKQDLRYGENPHQRA
HHCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHCCCCCCCCCCE
VFYRETNLKEPSITTAEKLHGKELSFNNINDANGALELVKEFTDRPAAAVIKHANPCGMA
EEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCHH
VADTLEEAYRKAHAGDPLSAFGSIVALNREVSPEVAREIAGKDKFVEVVIAPDYMTEALE
HHHHHHHHHHHHCCCCCHHHHHHEEEECCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHH
ILKNRWKNVRLLRTGDLFINREKPGYDMKKVTGGLLVQERDLAIINEDDLKIVTEVKPTE
HHHHHCCCEEEEEECCEEEECCCCCCCHHHHCCCEEEEECCEEEECCCCCEEEEECCCCH
GQLRDLLFAWKVVKHVKSNAIVMAKDEMVVGVGAGQMSRVDAMIIAGRKSEGRQEGGVVA
HHHHHHHHHHHHHHHHCCCEEEEEECCEEEEECCCCHHHCEEEEEECCCCCCCCCCCEEE
SDAFFPFPDAIEEAAARGIKAIIQPGGSIRDDQVIEAANDHKIAMVFTGRRHFRH
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEEEEECCHHCCC
>Mature Secondary Structure 
AKRALLSVSDKRGIVKMARGLVDLGYEIISTGGTARKLREGGLEVIDVSQITRFPEMMD
CCCCEEECCCCCCHHHHHHHHHHHCHHHHHCCCHHHHHHHCCCEEEEHHHHHHCHHHHC
GRVKTLHPAIHGGLLALRDNSGHMEQLEEQGIKPIDMVVVNLYPFAETISRPGVTLEEAI
CCHHHCCHHHHCCEEEEECCCCHHHHHHHCCCCEEHEEEEEHHHHHHHHCCCCCCHHHHH
ENIDIGGPSMIRSAAKNYCSVAVVTDPDDYDKILTELNENDGDLSLKTRQRLAVKAFRMT
HHCCCCCHHHHHHHHHCCEEEEEEECCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHH
AEYDTTIFDYLQGRNERDKKETSKAKSSKEEADIVFPEVISLNYIKKQDLRYGENPHQRA
HHCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHCCCCCCCCCCE
VFYRETNLKEPSITTAEKLHGKELSFNNINDANGALELVKEFTDRPAAAVIKHANPCGMA
EEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCHH
VADTLEEAYRKAHAGDPLSAFGSIVALNREVSPEVAREIAGKDKFVEVVIAPDYMTEALE
HHHHHHHHHHHHCCCCCHHHHHHEEEECCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHH
ILKNRWKNVRLLRTGDLFINREKPGYDMKKVTGGLLVQERDLAIINEDDLKIVTEVKPTE
HHHHHCCCEEEEEECCEEEECCCCCCCHHHHCCCEEEEECCEEEECCCCCEEEEECCCCH
GQLRDLLFAWKVVKHVKSNAIVMAKDEMVVGVGAGQMSRVDAMIIAGRKSEGRQEGGVVA
HHHHHHHHHHHHHHHHCCCEEEEEECCEEEEECCCCHHHCEEEEEECCCCCCCCCCCEEE
SDAFFPFPDAIEEAAARGIKAIIQPGGSIRDDQVIEAANDHKIAMVFTGRRHFRH
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA