Click here to switch to the map view.

The map label for this gene is opuAB [H]

Identifier: 220932982

GI number: 220932982

Start: 2339530

End: 2340348

Strand: Reverse

Name: opuAB [H]

Synonym: Hore_21500

Alternate gene names: 220932982

Gene position: 2340348-2339530 (Counterclockwise)

Preceding gene: 220932983

Following gene: 220932981

Centisome position: 90.78

GC content: 41.39

Gene sequence:

>819_bases
ATGTTTAGAATACCAGTTGGAAATCTCTTTGAACAGGTGGTAGAGTGGTTAAGTGAAAATGCTACCATAATATTTGATGT
TATCTCAGCCATTATAGATTTTTTCGTAGTAAATATTCAGAACCTGTTGTTTGTGGTACATCCTGTGGCAATAATTATTA
TCCTGGCAGCTCTGGCCTGGTATTTAGCCGGTCGGGGAGTAGCCATATTTACTATAGCTGGCTTTTTGATGATAATGGGC
ATGGACCTCTGGACCCAGACAATAGAAACCCTGTCCATGATTTTAACATCGGGGATAGTAGCTTTAAGTGTCGGGGTTTT
AATGGGTATCCTGGCTGCCAGAAATGATTATGTGGATAGAATCATAAGACCAGTTCTGGATTTTATGCAGACCATGCCGA
GTTTTGTCTACCTCATACCTGCGGTAATCTTTTTTGGCATGGAGATGGTTCCCGGAGTGATTGCTACAGTTATTTTCTCT
ATGCCACCTGCTATCAGGTTAACAAATCTTGGTATCAGGCAGGTTGATAAAGAGGTTATTGAAGCTGCGAGGGCCTTTGG
ATCGACCCCGAAGCAGATACTCTTCAAAGTCCAGTTACCACTGGCGGTACCAACAATAATGGCTGGAATTAACCAGACGA
TTATGCTATCACTATCTATGGTTGTAATTGCCTCAATGATTGGAGCCGGTGGTTTAGGTGGAATTGTATTAAGGGGTATT
ACCCAGCTGAAAATAGGTCTGGGATTTGAAGGTGGTCTTGCCGTTGTTATTCTGGCTATATTTCTTGATAGATTAACCCA
GAGTTTAAAAGAACTATAA

Upstream 100 bases:

>100_bases
ACTAAATATCCTGTTGCTGTCGTAAATGATGATAATCGTTTAACTGGAATAATAGTCAGGATTTCAGTCTTATCTGCCCT
GGCAGAAGGAGGGGAAGAAG

Downstream 100 bases:

>100_bases
GGGAGTTAAAAAAAACGATAAGAACATAAGGAGGTATAAGAAATGTTTAAAAAAAGATTATTGATTTTTATTGCTATAAG
TCTTAGCTTACTTCTATTAA

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MFRIPVGNLFEQVVEWLSENATIIFDVISAIIDFFVVNIQNLLFVVHPVAIIIILAALAWYLAGRGVAIFTIAGFLMIMG
MDLWTQTIETLSMILTSGIVALSVGVLMGILAARNDYVDRIIRPVLDFMQTMPSFVYLIPAVIFFGMEMVPGVIATVIFS
MPPAIRLTNLGIRQVDKEVIEAARAFGSTPKQILFKVQLPLAVPTIMAGINQTIMLSLSMVVIASMIGAGGLGGIVLRGI
TQLKIGLGFEGGLAVVILAIFLDRLTQSLKEL

Sequences:

>Translated_272_residues
MFRIPVGNLFEQVVEWLSENATIIFDVISAIIDFFVVNIQNLLFVVHPVAIIIILAALAWYLAGRGVAIFTIAGFLMIMG
MDLWTQTIETLSMILTSGIVALSVGVLMGILAARNDYVDRIIRPVLDFMQTMPSFVYLIPAVIFFGMEMVPGVIATVIFS
MPPAIRLTNLGIRQVDKEVIEAARAFGSTPKQILFKVQLPLAVPTIMAGINQTIMLSLSMVVIASMIGAGGLGGIVLRGI
TQLKIGLGFEGGLAVVILAIFLDRLTQSLKEL
>Mature_272_residues
MFRIPVGNLFEQVVEWLSENATIIFDVISAIIDFFVVNIQNLLFVVHPVAIIIILAALAWYLAGRGVAIFTIAGFLMIMG
MDLWTQTIETLSMILTSGIVALSVGVLMGILAARNDYVDRIIRPVLDFMQTMPSFVYLIPAVIFFGMEMVPGVIATVIFS
MPPAIRLTNLGIRQVDKEVIEAARAFGSTPKQILFKVQLPLAVPTIMAGINQTIMLSLSMVVIASMIGAGGLGGIVLRGI
TQLKIGLGFEGGLAVVILAIFLDRLTQSLKEL

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=268, Percent_Identity=47.3880597014925, Blast_Score=234, Evalue=3e-63,
Organism=Escherichia coli, GI1788451, Length=193, Percent_Identity=30.5699481865285, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788449, Length=179, Percent_Identity=30.1675977653631, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29495; Mature: 29495

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]