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The map label for this gene is glnA [H]

Identifier: 220932961

GI number: 220932961

Start: 2317814

End: 2319139

Strand: Reverse

Name: glnA [H]

Synonym: Hore_21290

Alternate gene names: 220932961

Gene position: 2319139-2317814 (Counterclockwise)

Preceding gene: 220932962

Following gene: 220932960

Centisome position: 89.95

GC content: 43.44

Gene sequence:

>1326_bases
ATGTCATTAACCAGGCAGGAAGTATTAAAGAAGGCAGAAGAGCTGAATGTAAAATTTATTCGACTCCAGTTTGTTGATAT
TTTAGGGACGATTAAAAATGTGGCTATTACTGTAGAACAGCTACCGGATGCCCTGGACGGGAAAATAATGTTTGATGGAT
CTTCAATAGAGGGTTTTACCAGGATTCAGGAATCAGATATGTACTTAAAACCGGATTATGATACCTTTACTATTTTCCCC
TGGCGTCCCCGTGAAGGTGCTGTAGCCAGATTGATGTGTGATATCTATACCCCGGACGGAGAGCCCTTTGCCGGATGTCC
CCGGAGCACCCTTAAAAAAGTTATTGATGAGGCCCGGGAGATGGGATATGAAATGTTTGCCGGACCGGAGCCTGAGTTTT
TTCTGTTTGAGAGGGATGAAAAGGGCCATCCAACAACTATTACCAATGATAAAGGTGGTTATTTTGATTTGTCACCCCTG
GATATGGGGGAGAATGCCAGGCGGGATATTGTACTGGCCCTCGAACAGATGGGATTCGATGTTGAGGCTTCTCATCATGA
AGTTGCTCCCGGCCAGCATGAAATTGACTTTAAATATACTCCAGTTCTAAGGACAGCTGATAACATAACCACTTTTAAAT
TTGTTACCAAAGCTATTGCCCTCAACCATAATTTACATGCCACTTTTATGCCGAAACCCATTTTTGGTGAAAATGGATCC
GGAATGCATGTCCACCAGTCCCTGTTCAAGGATGGGGAAAATGCTTTTTATGAACCTGATGATGAACTGGGATTATCAAA
GATAGCCTACCATTATATTGGAGGTGTTTTAAAACACGCTCCCGCCATAACTGCTATAACTAATCCAACTATTAATTCCT
ATAAAAGACTTGTTCCTGGCTACGAAGCCCCGGTTTATATCTCCTGGTCTGCCCAGAACCGGAGTGCCCTGATCAGGGTG
CCATCAGCCCGGGGTAATGGTACCAGAATTGAGCTCAGAAATCCCGATCCCTCGGCCAACCCCTACCTGGCCATGGCTGT
AATGTTAAAAGCCGGTCTGGATGGTATAAAAAACGAGATAGATCCGGGAGAGCAGACCCTTGATGATATTTTCTCCATGT
CAGATGAAGAGCGAAAGGCAAGGGGAATTGAAAGCCTGCCAGGTAATATAATGGAGGCTGTCAATAACCTGAAAGAAGAT
AAGTTAATAAAAGAAACTCTGGGAGAACATATATTTAATCATTTTGTGGAAGCTAAAGAAATAGAATGGGCTGTTTATCG
AACCCAGGTTCATCAGTGGGAACTGGAGCAATACCTATCTTTCTAA

Upstream 100 bases:

>100_bases
AGCTTAACAAAAATCCCCTGTCATCACTCTATCCTGTTTCTAACAGGTATAAGCTAAATAAGATTCTTGGAAAAAAGAAA
GAATTAAAGGAGGAAAAATA

Downstream 100 bases:

>100_bases
AATATACGACCAGGGAGGGTAAGTTATGCCTGACAGAAGAATAGTTAAAGAGTTGAGGAATTTAGAACCGGGGAATCTGT
CAACCCGTACTTTATCATAT

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSLTRQEVLKKAEELNVKFIRLQFVDILGTIKNVAITVEQLPDALDGKIMFDGSSIEGFTRIQESDMYLKPDYDTFTIFP
WRPREGAVARLMCDIYTPDGEPFAGCPRSTLKKVIDEAREMGYEMFAGPEPEFFLFERDEKGHPTTITNDKGGYFDLSPL
DMGENARRDIVLALEQMGFDVEASHHEVAPGQHEIDFKYTPVLRTADNITTFKFVTKAIALNHNLHATFMPKPIFGENGS
GMHVHQSLFKDGENAFYEPDDELGLSKIAYHYIGGVLKHAPAITAITNPTINSYKRLVPGYEAPVYISWSAQNRSALIRV
PSARGNGTRIELRNPDPSANPYLAMAVMLKAGLDGIKNEIDPGEQTLDDIFSMSDEERKARGIESLPGNIMEAVNNLKED
KLIKETLGEHIFNHFVEAKEIEWAVYRTQVHQWELEQYLSF

Sequences:

>Translated_441_residues
MSLTRQEVLKKAEELNVKFIRLQFVDILGTIKNVAITVEQLPDALDGKIMFDGSSIEGFTRIQESDMYLKPDYDTFTIFP
WRPREGAVARLMCDIYTPDGEPFAGCPRSTLKKVIDEAREMGYEMFAGPEPEFFLFERDEKGHPTTITNDKGGYFDLSPL
DMGENARRDIVLALEQMGFDVEASHHEVAPGQHEIDFKYTPVLRTADNITTFKFVTKAIALNHNLHATFMPKPIFGENGS
GMHVHQSLFKDGENAFYEPDDELGLSKIAYHYIGGVLKHAPAITAITNPTINSYKRLVPGYEAPVYISWSAQNRSALIRV
PSARGNGTRIELRNPDPSANPYLAMAVMLKAGLDGIKNEIDPGEQTLDDIFSMSDEERKARGIESLPGNIMEAVNNLKED
KLIKETLGEHIFNHFVEAKEIEWAVYRTQVHQWELEQYLSF
>Mature_440_residues
SLTRQEVLKKAEELNVKFIRLQFVDILGTIKNVAITVEQLPDALDGKIMFDGSSIEGFTRIQESDMYLKPDYDTFTIFPW
RPREGAVARLMCDIYTPDGEPFAGCPRSTLKKVIDEAREMGYEMFAGPEPEFFLFERDEKGHPTTITNDKGGYFDLSPLD
MGENARRDIVLALEQMGFDVEASHHEVAPGQHEIDFKYTPVLRTADNITTFKFVTKAIALNHNLHATFMPKPIFGENGSG
MHVHQSLFKDGENAFYEPDDELGLSKIAYHYIGGVLKHAPAITAITNPTINSYKRLVPGYEAPVYISWSAQNRSALIRVP
SARGNGTRIELRNPDPSANPYLAMAVMLKAGLDGIKNEIDPGEQTLDDIFSMSDEERKARGIESLPGNIMEAVNNLKEDK
LIKETLGEHIFNHFVEAKEIEWAVYRTQVHQWELEQYLSF

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=364, Percent_Identity=26.6483516483516, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1790301, Length=477, Percent_Identity=41.2997903563941, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI87081870, Length=367, Percent_Identity=26.9754768392371, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI115532978, Length=323, Percent_Identity=26.0061919504644, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115532980, Length=323, Percent_Identity=26.0061919504644, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 49830; Mature: 49699

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTRQEVLKKAEELNVKFIRLQFVDILGTIKNVAITVEQLPDALDGKIMFDGSSIEGFT
CCCCHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHEEEHHHCCCCCCCEEEEECCCCCHHH
RIQESDMYLKPDYDTFTIFPWRPREGAVARLMCDIYTPDGEPFAGCPRSTLKKVIDEARE
EEECCCCEECCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
MGYEMFAGPEPEFFLFERDEKGHPTTITNDKGGYFDLSPLDMGENARRDIVLALEQMGFD
CCCCHHCCCCCCEEEEECCCCCCCCEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHCCC
VEASHHEVAPGQHEIDFKYTPVLRTADNITTFKFVTKAIALNHNLHATFMPKPIFGENGS
CCCCCCCCCCCCEECCEEECCCEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
GMHVHQSLFKDGENAFYEPDDELGLSKIAYHYIGGVLKHAPAITAITNPTINSYKRLVPG
CCHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCC
YEAPVYISWSAQNRSALIRVPSARGNGTRIELRNPDPSANPYLAMAVMLKAGLDGIKNEI
CCCCEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCHHHHHHCC
DPGEQTLDDIFSMSDEERKARGIESLPGNIMEAVNNLKEDKLIKETLGEHIFNHFVEAKE
CCCHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEWAVYRTQVHQWELEQYLSF
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLTRQEVLKKAEELNVKFIRLQFVDILGTIKNVAITVEQLPDALDGKIMFDGSSIEGFT
CCCHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHEEEHHHCCCCCCCEEEEECCCCCHHH
RIQESDMYLKPDYDTFTIFPWRPREGAVARLMCDIYTPDGEPFAGCPRSTLKKVIDEARE
EEECCCCEECCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
MGYEMFAGPEPEFFLFERDEKGHPTTITNDKGGYFDLSPLDMGENARRDIVLALEQMGFD
CCCCHHCCCCCCEEEEECCCCCCCCEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHCCC
VEASHHEVAPGQHEIDFKYTPVLRTADNITTFKFVTKAIALNHNLHATFMPKPIFGENGS
CCCCCCCCCCCCEECCEEECCCEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
GMHVHQSLFKDGENAFYEPDDELGLSKIAYHYIGGVLKHAPAITAITNPTINSYKRLVPG
CCHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCC
YEAPVYISWSAQNRSALIRVPSARGNGTRIELRNPDPSANPYLAMAVMLKAGLDGIKNEI
CCCCEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCHHHHHHCC
DPGEQTLDDIFSMSDEERKARGIESLPGNIMEAVNNLKEDKLIKETLGEHIFNHFVEAKE
CCCHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEWAVYRTQVHQWELEQYLSF
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2891680; 1981087 [H]