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The map label for this gene is lacZ [H]

Identifier: 220932881

GI number: 220932881

Start: 2211551

End: 2213803

Strand: Reverse

Name: lacZ [H]

Synonym: Hore_20490

Alternate gene names: 220932881

Gene position: 2213803-2211551 (Counterclockwise)

Preceding gene: 220932882

Following gene: 220932880

Centisome position: 85.87

GC content: 42.3

Gene sequence:

>2253_bases
ATGAAACGGAAAATATTAAACTTTAATACGGACTGGTTTTTTCTTGATAAGGATATAGAAGGAGCAAAAGGAATAGATTT
TAGTCAATCAGGGATGGAGAAAGTAAATCTACCTCATCCCAACAGGATTTTACCACACCATTATTTTGAAGAATCTGATT
ATCAGTTTGTTTCCTGGTACAGGCGCCCTTTTTACCTGGAAGAAGAGTATAAAGGGAAAAGGGTGATAGTAGAGTTTGAT
GGAGTAATGACGGTTGCTGAAATATATGTTAATGGGCAGTTTGTGGGTGAGCATAAAGGGGGCTATACTTCTTTTAGTTT
TGATATAACAGATTATCTGCTATCTGGAGAAAATAATCTGCTGGCAGTCAGGGTGGATTCCAGCCAGAGAAAGGATATTC
CCCCGGAAGGCAACCTGGTCGATTACCTTTTGTTTGGAGGTATATACCGGGATGTTAAAATGGTGATAGTAGACCCTGTT
TATATTAACTGGTCTTTTATTGAACTTAAAGATGTAAACCTGGAAGCAGGTGTTATAAAACCCAGGTTTGAGCTCGTAAA
CACAACAGGTAACCGGCAAAAAATAGTCTTAAATAGTCAGGTTATTAATAAAGAAGGTAAGGTTGTGGCAATGGTAGAAT
CCAGACACCTGCTTGAGCCCGGTGTAACCTCTCTGGAGCAGCCTGAGGTCAAGATAAAAGAACCTGAGTTATGGCATCCT
GACCATCCCTATCTCTATCATGTTTATACTGAAGTTAAAGTTGAAGGAAAGCTGGTTGATGATTATAAAACCAGAATCGG
ACTCAGGAAAGTGGAATTTAAAGAGGATGGAAAGTTTTATATCAACGATAAGCCCCTTAAACTCAGGGGACTTAACAGGC
ATCAAATGTTTCCGTATCTAGGTAATGCTATGCCTGACCGGGGCCAGAGGAAGGATGCTGAGATTTTGAAGTATGAACTG
GGGTTAAATTTTGTCCGTTCTTCCCATTACCCGGCTGATTCTTCGTTTTTAGATAAGTGTGATGAAATAGGTTTATTAGT
CCTGGAAGAGATCCCCGGATGGCAGCATATCGGGAACAGGGACTGGCAGGAGTTATCTAAAAGAAATGTTGAAGAGATGA
TAGTCAGAGACCGGAACCATCCCTGTATTTTCCTCTGGGGTGTTAGAATTAATGAATCTCCGGATAACCATGATTTTTAC
CTTGAGACAAATGAAATTGCCCACAGACTGGACAGTACCAGGCCGACCTGTGGGATAAGGAATTTTCAGGATAGTGAGTT
TCTGGAAGATGTATTTACTTATAATGATTTTGAGTTAAATCTCGAAGGAAAAATTAAATTACCTAACCACCAACCATATA
TGATAACCGAATATATGGGTCATATGTATCCAACCAAGGCCTATGATAGTGTCGAAAGGTTAATTAAACACGCTGTCCGG
CACGCCCATATACAGGATAAGCAGTATGGGGTACCTTATCTGGCAGGGGCCTCAGGGTGGTGTGCCTTTGATTATAATAC
CCATGCTGATTTTGGATCAGGTGACAGGGTATGCTATCACGGAGTCTGTGATATGTTCAGGTTACCCAAATTTGCTGCTT
ATTTTTATAAAAGCCAGATAGACCCGGATGTGGAAAAGGTTGTATTTATTGCTCGATACCTGACCCCATCTTTTAATGAG
GATTATGGCGATGAGGTTATTGTTTTTAGTAACTGTGAAGAGGTTGAACTATATGTTGGTGATAAATTAATAACATCAGC
TAGACCAAACCGGGTTGATTACCCCAGTTTACCCCACCCGCCCTTTACCTTTAAAGACTGTACCTGGTGGGAGTGGGGGG
CCAGCACCATTTCCTGCCTGAAAGCGGTCGGTAAAATAGATGGGAAACAGGTTGCCGAGCACACTATTTATCCCTTTGGC
AGGCCGGAGAGGTTAGTATTAAAGCCGGATTACACTAAACTTACGGCAGATGGTGCTGATTGTACCCGGGTTGTGGTTGA
GCTTCAGGATGAGCACGGACAGGTCCTCCATCTGGCCCATCATCCGGTTTTCTTTGAACTGGAAGGGGTGGGGGAACTAA
TTGGAGAAAACCCCTTTAGCCTGGAAGTAGGGAGAGGTGCTGTCTTTATAAGGGCCGGGAGAACTCCAGGGAAAATACAG
CTGACAGGTAAGGTCCAGGGATTACCACCGGTCACAATAGTTGTATCTACTGAACCTCTGGAAGATAAGATAGTACCATT
ACCCAGGAAATAA

Upstream 100 bases:

>100_bases
GTACTTTATTGAAGGTTTAACAATGGGATCCGGCAAATAAAAAACTAGTTTAGGTATTGCAAACAGTGATTTTTGTGGAT
AACAGGGGAGGTTATTTTTA

Downstream 100 bases:

>100_bases
TTGAAAGGCAAGCAGTTAACCGTTTTTTGCGGTTAACTGTTTTTTTATGCTCATTCCTTGAATAACTTGACATTGGGGAC
CTTCTTTGTTAAAATAGCTT

Product: Beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 750; Mature: 750

Protein sequence:

>750_residues
MKRKILNFNTDWFFLDKDIEGAKGIDFSQSGMEKVNLPHPNRILPHHYFEESDYQFVSWYRRPFYLEEEYKGKRVIVEFD
GVMTVAEIYVNGQFVGEHKGGYTSFSFDITDYLLSGENNLLAVRVDSSQRKDIPPEGNLVDYLLFGGIYRDVKMVIVDPV
YINWSFIELKDVNLEAGVIKPRFELVNTTGNRQKIVLNSQVINKEGKVVAMVESRHLLEPGVTSLEQPEVKIKEPELWHP
DHPYLYHVYTEVKVEGKLVDDYKTRIGLRKVEFKEDGKFYINDKPLKLRGLNRHQMFPYLGNAMPDRGQRKDAEILKYEL
GLNFVRSSHYPADSSFLDKCDEIGLLVLEEIPGWQHIGNRDWQELSKRNVEEMIVRDRNHPCIFLWGVRINESPDNHDFY
LETNEIAHRLDSTRPTCGIRNFQDSEFLEDVFTYNDFELNLEGKIKLPNHQPYMITEYMGHMYPTKAYDSVERLIKHAVR
HAHIQDKQYGVPYLAGASGWCAFDYNTHADFGSGDRVCYHGVCDMFRLPKFAAYFYKSQIDPDVEKVVFIARYLTPSFNE
DYGDEVIVFSNCEEVELYVGDKLITSARPNRVDYPSLPHPPFTFKDCTWWEWGASTISCLKAVGKIDGKQVAEHTIYPFG
RPERLVLKPDYTKLTADGADCTRVVVELQDEHGQVLHLAHHPVFFELEGVGELIGENPFSLEVGRGAVFIRAGRTPGKIQ
LTGKVQGLPPVTIVVSTEPLEDKIVPLPRK

Sequences:

>Translated_750_residues
MKRKILNFNTDWFFLDKDIEGAKGIDFSQSGMEKVNLPHPNRILPHHYFEESDYQFVSWYRRPFYLEEEYKGKRVIVEFD
GVMTVAEIYVNGQFVGEHKGGYTSFSFDITDYLLSGENNLLAVRVDSSQRKDIPPEGNLVDYLLFGGIYRDVKMVIVDPV
YINWSFIELKDVNLEAGVIKPRFELVNTTGNRQKIVLNSQVINKEGKVVAMVESRHLLEPGVTSLEQPEVKIKEPELWHP
DHPYLYHVYTEVKVEGKLVDDYKTRIGLRKVEFKEDGKFYINDKPLKLRGLNRHQMFPYLGNAMPDRGQRKDAEILKYEL
GLNFVRSSHYPADSSFLDKCDEIGLLVLEEIPGWQHIGNRDWQELSKRNVEEMIVRDRNHPCIFLWGVRINESPDNHDFY
LETNEIAHRLDSTRPTCGIRNFQDSEFLEDVFTYNDFELNLEGKIKLPNHQPYMITEYMGHMYPTKAYDSVERLIKHAVR
HAHIQDKQYGVPYLAGASGWCAFDYNTHADFGSGDRVCYHGVCDMFRLPKFAAYFYKSQIDPDVEKVVFIARYLTPSFNE
DYGDEVIVFSNCEEVELYVGDKLITSARPNRVDYPSLPHPPFTFKDCTWWEWGASTISCLKAVGKIDGKQVAEHTIYPFG
RPERLVLKPDYTKLTADGADCTRVVVELQDEHGQVLHLAHHPVFFELEGVGELIGENPFSLEVGRGAVFIRAGRTPGKIQ
LTGKVQGLPPVTIVVSTEPLEDKIVPLPRK
>Mature_750_residues
MKRKILNFNTDWFFLDKDIEGAKGIDFSQSGMEKVNLPHPNRILPHHYFEESDYQFVSWYRRPFYLEEEYKGKRVIVEFD
GVMTVAEIYVNGQFVGEHKGGYTSFSFDITDYLLSGENNLLAVRVDSSQRKDIPPEGNLVDYLLFGGIYRDVKMVIVDPV
YINWSFIELKDVNLEAGVIKPRFELVNTTGNRQKIVLNSQVINKEGKVVAMVESRHLLEPGVTSLEQPEVKIKEPELWHP
DHPYLYHVYTEVKVEGKLVDDYKTRIGLRKVEFKEDGKFYINDKPLKLRGLNRHQMFPYLGNAMPDRGQRKDAEILKYEL
GLNFVRSSHYPADSSFLDKCDEIGLLVLEEIPGWQHIGNRDWQELSKRNVEEMIVRDRNHPCIFLWGVRINESPDNHDFY
LETNEIAHRLDSTRPTCGIRNFQDSEFLEDVFTYNDFELNLEGKIKLPNHQPYMITEYMGHMYPTKAYDSVERLIKHAVR
HAHIQDKQYGVPYLAGASGWCAFDYNTHADFGSGDRVCYHGVCDMFRLPKFAAYFYKSQIDPDVEKVVFIARYLTPSFNE
DYGDEVIVFSNCEEVELYVGDKLITSARPNRVDYPSLPHPPFTFKDCTWWEWGASTISCLKAVGKIDGKQVAEHTIYPFG
RPERLVLKPDYTKLTADGADCTRVVVELQDEHGQVLHLAHHPVFFELEGVGELIGENPFSLEVGRGAVFIRAGRTPGKIQ
LTGKVQGLPPVTIVVSTEPLEDKIVPLPRK

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=401, Percent_Identity=25.9351620947631, Blast_Score=110, Evalue=4e-24,
Organism=Escherichia coli, GI48994920, Length=353, Percent_Identity=31.1614730878187, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1787903, Length=463, Percent_Identity=25.4859611231102, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1786539, Length=359, Percent_Identity=25.6267409470752, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17510825, Length=407, Percent_Identity=23.3415233415233, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI62471735, Length=542, Percent_Identity=24.5387453874539, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI19922582, Length=542, Percent_Identity=24.5387453874539, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24655438, Length=542, Percent_Identity=24.5387453874539, Blast_Score=132, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24651745, Length=436, Percent_Identity=25.2293577981651, Blast_Score=116, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 86490; Mature: 86490

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00719 GLYCOSYL_HYDROL_F2_1 ; PS00608 GLYCOSYL_HYDROL_F2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKILNFNTDWFFLDKDIEGAKGIDFSQSGMEKVNLPHPNRILPHHYFEESDYQFVSWY
CCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
RRPFYLEEEYKGKRVIVEFDGVMTVAEIYVNGQFVGEHKGGYTSFSFDITDYLLSGENNL
CCCCEECCCCCCCEEEEEECCCEEEEEEEECCEEEECCCCCEEEEEEEHEEHEECCCCCE
LAVRVDSSQRKDIPPEGNLVDYLLFGGIYRDVKMVIVDPVYINWSFIELKDVNLEAGVIK
EEEEECCCCCCCCCCCCCEEHHHHHCCHHHCEEEEEEEEEEEEEEEEEEEECCEECCEEC
PRFELVNTTGNRQKIVLNSQVINKEGKVVAMVESRHLLEPGVTSLEQPEVKIKEPELWHP
CCEEEEECCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCC
DHPYLYHVYTEVKVEGKLVDDYKTRIGLRKVEFKEDGKFYINDKPLKLRGLNRHQMFPYL
CCCEEEEEEEEEEEECEEECCHHHHCCCEEEEEECCCCEEECCCCEEEECCCCCCCCHHH
GNAMPDRGQRKDAEILKYELGLNFVRSSHYPADSSFLDKCDEIGLLVLEEIPGWQHIGNR
CCCCCCCCCCCCHHHEEEECCCCEEECCCCCCCCHHHHHHHHCCEEEEECCCCHHHCCCC
DWQELSKRNVEEMIVRDRNHPCIFLWGVRINESPDNHDFYLETNEIAHRLDSTRPTCGIR
CHHHHHHCCHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEEHHHHHHHHCCCCCCCCCC
NFQDSEFLEDVFTYNDFELNLEGKIKLPNHQPYMITEYMGHMYPTKAYDSVERLIKHAVR
CCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHHH
HAHIQDKQYGVPYLAGASGWCAFDYNTHADFGSGDRVCYHGVCDMFRLPKFAAYFYKSQI
HCCCCCCCCCCCEEECCCCEEEECCCCCCCCCCCCCEEEHHHHHHHHCHHHHHHHHHCCC
DPDVEKVVFIARYLTPSFNEDYGDEVIVFSNCEEVELYVGDKLITSARPNRVDYPSLPHP
CCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCEEEECCCCCCCCCCCCCCC
PFTFKDCTWWEWGASTISCLKAVGKIDGKQVAEHTIYPFGRPERLVLKPDYTKLTADGAD
CCCCCCCCCEECCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEECCCCCEEECCCCC
CTRVVVELQDEHGQVLHLAHHPVFFELEGVGELIGENPFSLEVGRGAVFIRAGRTPGKIQ
CEEEEEEEECCCCCEEEEECCCEEEEECCHHHHHCCCCCEEEECCCEEEEEECCCCCEEE
LTGKVQGLPPVTIVVSTEPLEDKIVPLPRK
EEEEECCCCCEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKRKILNFNTDWFFLDKDIEGAKGIDFSQSGMEKVNLPHPNRILPHHYFEESDYQFVSWY
CCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
RRPFYLEEEYKGKRVIVEFDGVMTVAEIYVNGQFVGEHKGGYTSFSFDITDYLLSGENNL
CCCCEECCCCCCCEEEEEECCCEEEEEEEECCEEEECCCCCEEEEEEEHEEHEECCCCCE
LAVRVDSSQRKDIPPEGNLVDYLLFGGIYRDVKMVIVDPVYINWSFIELKDVNLEAGVIK
EEEEECCCCCCCCCCCCCEEHHHHHCCHHHCEEEEEEEEEEEEEEEEEEEECCEECCEEC
PRFELVNTTGNRQKIVLNSQVINKEGKVVAMVESRHLLEPGVTSLEQPEVKIKEPELWHP
CCEEEEECCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCC
DHPYLYHVYTEVKVEGKLVDDYKTRIGLRKVEFKEDGKFYINDKPLKLRGLNRHQMFPYL
CCCEEEEEEEEEEEECEEECCHHHHCCCEEEEEECCCCEEECCCCEEEECCCCCCCCHHH
GNAMPDRGQRKDAEILKYELGLNFVRSSHYPADSSFLDKCDEIGLLVLEEIPGWQHIGNR
CCCCCCCCCCCCHHHEEEECCCCEEECCCCCCCCHHHHHHHHCCEEEEECCCCHHHCCCC
DWQELSKRNVEEMIVRDRNHPCIFLWGVRINESPDNHDFYLETNEIAHRLDSTRPTCGIR
CHHHHHHCCHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEEHHHHHHHHCCCCCCCCCC
NFQDSEFLEDVFTYNDFELNLEGKIKLPNHQPYMITEYMGHMYPTKAYDSVERLIKHAVR
CCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHHH
HAHIQDKQYGVPYLAGASGWCAFDYNTHADFGSGDRVCYHGVCDMFRLPKFAAYFYKSQI
HCCCCCCCCCCCEEECCCCEEEECCCCCCCCCCCCCEEEHHHHHHHHCHHHHHHHHHCCC
DPDVEKVVFIARYLTPSFNEDYGDEVIVFSNCEEVELYVGDKLITSARPNRVDYPSLPHP
CCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCEEEECCCCCCCCCCCCCCC
PFTFKDCTWWEWGASTISCLKAVGKIDGKQVAEHTIYPFGRPERLVLKPDYTKLTADGAD
CCCCCCCCCEECCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEECCCCCEEECCCCC
CTRVVVELQDEHGQVLHLAHHPVFFELEGVGELIGENPFSLEVGRGAVFIRAGRTPGKIQ
CEEEEEEEECCCCCEEEEECCCEEEEECCHHHHHCCCCCEEEECCCEEEEEECCCCCEEE
LTGKVQGLPPVTIVVSTEPLEDKIVPLPRK
EEEEECCCCCEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA