Click here to switch to the map view.

The map label for this gene is malE [H]

Identifier: 220932879

GI number: 220932879

Start: 2208237

End: 2209457

Strand: Reverse

Name: malE [H]

Synonym: Hore_20470

Alternate gene names: 220932879

Gene position: 2209457-2208237 (Counterclockwise)

Preceding gene: 220932880

Following gene: 220932878

Centisome position: 85.7

GC content: 40.38

Gene sequence:

>1221_bases
ATGAAAAAGGTTGCTTTACTTGTTGTTACTTTAATGCTTATTTTTACACTGCCGACCATGGCGGTAACCAAATTAACTAT
CTGGGAAAAATATGATGAGGCTACCCAGGATCCATATTTCCAGAGTTTAGTAGATGAATTTAACAAAACCCATGAAGACA
TTGAAGTTGAAATGCTCCACTATAATACTGAAGATTTGAGGGAAAACTTCCAGAACGCTATGATGGCCGGAGAAGGTCCT
GATGCTACCATCAGTCCCTTTGACCATGTCGGTGTTTTTGCCGTTTTAGGTATTGCAGAGCCAATTACTGATATTTTACC
ACAGGACATTAAAGATGCCCTGGTTGACAATGCTTTACCTGCCATGAGCCTTGATGGAGAAATGTACGGTGTTCCTATTG
ATATGGGTAACCACTTAATGCTACTTTATAATAAGAAATTTGTCAAAGAACCACCCAAAACCTGGGACGAGTTAATTAAA
ATTGCCAAAAAACATACTAAAGACCTGGACGGTGACGGAGTAAATGACCAGTTTGGTCTGGTTTATAACCTGACTGAACC
TTTCTGGTTTATTCCTTTCATGAGTGGTCACGGTGGCTGGGTTATGGACGAAAACCGTCAGCCAACCTTAAATACTGATG
GTGTAGTTAATGCCTTTAAATTTATCAGGGATCTTAAATTTGAACATAAAATTGTCCCTGAAGAATGTGACTATGATATT
GCAGATAGCCTCTTTAAAGAAAATAAAGCCGTATTTCTTATCAATGGTGACTGGTCCTTAAATGGTTATAAAGCAGTAGA
AGGCCTTGATTTTGGTACAGCAGCCATACCTAAATTCAAAGATTATGAGTGGCCTAAACCGATGATGAGTGGTAATGGTT
TCATCATGGCCGAAGGTTTATCTGAAGAAAAGCAGGAGGCCCTCTTTGAATTTATCCGCTTTGTACTGGAGAAAGAAAAC
CAGGTTCGTATGGTAAAAGAACTGAGTATCCTTCCCAGTACCAAAGCTGCCAGAGAAGTAGAGTTTGAAGACCCGATATT
AAGGGGTTCTATTGAACAGCTTAACCATACAAAACCAATGCCTATTGTTCCTGAAATGAGGGCGATCTGGGATGCCCTGA
GAGCACCAATCCAGAATGTTATGAATGGTAGTGCTACTCCTGAAGATGCTGCCAAAGAAGCTCAGGATCTGGCTGAAGAA
GGCGTAGGTGCAATGCATTAA

Upstream 100 bases:

>100_bases
TTCTTTATTCAAACGTTTGCTTCTTTTCTTAATTCTCCATTCAAACGTTTTCTAAAACCATTCAAACGTTTTCCAAAAAA
ATTAAAGGAGGAGTTTGTTA

Downstream 100 bases:

>100_bases
AATAATGTGGGCTTAAGTTAGAGAGGAGTTAACCGGGTTTACCCGGTTAACTCTTCTTTTCAATAAGATGAGAGAATAGG
AAGAAGGGGGTTACTGTGAG

Product: family 1 extracellular solute-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKKVALLVVTLMLIFTLPTMAVTKLTIWEKYDEATQDPYFQSLVDEFNKTHEDIEVEMLHYNTEDLRENFQNAMMAGEGP
DATISPFDHVGVFAVLGIAEPITDILPQDIKDALVDNALPAMSLDGEMYGVPIDMGNHLMLLYNKKFVKEPPKTWDELIK
IAKKHTKDLDGDGVNDQFGLVYNLTEPFWFIPFMSGHGGWVMDENRQPTLNTDGVVNAFKFIRDLKFEHKIVPEECDYDI
ADSLFKENKAVFLINGDWSLNGYKAVEGLDFGTAAIPKFKDYEWPKPMMSGNGFIMAEGLSEEKQEALFEFIRFVLEKEN
QVRMVKELSILPSTKAAREVEFEDPILRGSIEQLNHTKPMPIVPEMRAIWDALRAPIQNVMNGSATPEDAAKEAQDLAEE
GVGAMH

Sequences:

>Translated_406_residues
MKKVALLVVTLMLIFTLPTMAVTKLTIWEKYDEATQDPYFQSLVDEFNKTHEDIEVEMLHYNTEDLRENFQNAMMAGEGP
DATISPFDHVGVFAVLGIAEPITDILPQDIKDALVDNALPAMSLDGEMYGVPIDMGNHLMLLYNKKFVKEPPKTWDELIK
IAKKHTKDLDGDGVNDQFGLVYNLTEPFWFIPFMSGHGGWVMDENRQPTLNTDGVVNAFKFIRDLKFEHKIVPEECDYDI
ADSLFKENKAVFLINGDWSLNGYKAVEGLDFGTAAIPKFKDYEWPKPMMSGNGFIMAEGLSEEKQEALFEFIRFVLEKEN
QVRMVKELSILPSTKAAREVEFEDPILRGSIEQLNHTKPMPIVPEMRAIWDALRAPIQNVMNGSATPEDAAKEAQDLAEE
GVGAMH
>Mature_406_residues
MKKVALLVVTLMLIFTLPTMAVTKLTIWEKYDEATQDPYFQSLVDEFNKTHEDIEVEMLHYNTEDLRENFQNAMMAGEGP
DATISPFDHVGVFAVLGIAEPITDILPQDIKDALVDNALPAMSLDGEMYGVPIDMGNHLMLLYNKKFVKEPPKTWDELIK
IAKKHTKDLDGDGVNDQFGLVYNLTEPFWFIPFMSGHGGWVMDENRQPTLNTDGVVNAFKFIRDLKFEHKIVPEECDYDI
ADSLFKENKAVFLINGDWSLNGYKAVEGLDFGTAAIPKFKDYEWPKPMMSGNGFIMAEGLSEEKQEALFEFIRFVLEKEN
QVRMVKELSILPSTKAAREVEFEDPILRGSIEQLNHTKPMPIVPEMRAIWDALRAPIQNVMNGSATPEDAAKEAQDLAEE
GVGAMH

Specific function: Involved in an abc transport system for maltotriose. Binds maltotriose much more tightly than maltose [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=406, Percent_Identity=26.3546798029557, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45931; Mature: 45931

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVALLVVTLMLIFTLPTMAVTKLTIWEKYDEATQDPYFQSLVDEFNKTHEDIEVEMLH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEE
YNTEDLRENFQNAMMAGEGPDATISPFDHVGVFAVLGIAEPITDILPQDIKDALVDNALP
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
AMSLDGEMYGVPIDMGNHLMLLYNKKFVKEPPKTWDELIKIAKKHTKDLDGDGVNDQFGL
CEECCCCEEEEEECCCCCEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCE
VYNLTEPFWFIPFMSGHGGWVMDENRQPTLNTDGVVNAFKFIRDLKFEHKIVPEECDYDI
EEECCCCEEEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHH
ADSLFKENKAVFLINGDWSLNGYKAVEGLDFGTAAIPKFKDYEWPKPMMSGNGFIMAEGL
HHHHHHCCCEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCC
SEEKQEALFEFIRFVLEKENQVRMVKELSILPSTKAAREVEFEDPILRGSIEQLNHTKPM
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHCCCCCCCHHHCCHHHHCCCCCC
PIVPEMRAIWDALRAPIQNVMNGSATPEDAAKEAQDLAEEGVGAMH
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKVALLVVTLMLIFTLPTMAVTKLTIWEKYDEATQDPYFQSLVDEFNKTHEDIEVEMLH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEE
YNTEDLRENFQNAMMAGEGPDATISPFDHVGVFAVLGIAEPITDILPQDIKDALVDNALP
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
AMSLDGEMYGVPIDMGNHLMLLYNKKFVKEPPKTWDELIKIAKKHTKDLDGDGVNDQFGL
CEECCCCEEEEEECCCCCEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCE
VYNLTEPFWFIPFMSGHGGWVMDENRQPTLNTDGVVNAFKFIRDLKFEHKIVPEECDYDI
EEECCCCEEEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHH
ADSLFKENKAVFLINGDWSLNGYKAVEGLDFGTAAIPKFKDYEWPKPMMSGNGFIMAEGL
HHHHHHCCCEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCC
SEEKQEALFEFIRFVLEKENQVRMVKELSILPSTKAAREVEFEDPILRGSIEQLNHTKPM
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHCCCCCCCHHHCCHHHHCCCCCC
PIVPEMRAIWDALRAPIQNVMNGSATPEDAAKEAQDLAEEGVGAMH
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11162100 [H]