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The map label for this gene is holB [H]

Identifier: 220932868

GI number: 220932868

Start: 2195498

End: 2196478

Strand: Reverse

Name: holB [H]

Synonym: Hore_20360

Alternate gene names: 220932868

Gene position: 2196478-2195498 (Counterclockwise)

Preceding gene: 220932869

Following gene: 220932867

Centisome position: 85.2

GC content: 33.94

Gene sequence:

>981_bases
ATGGGGTTTAATGAAATTATCGGGCAAAATGGGGCTATAAAGATTTTAAAGGAAGAATTAAGGGATAATAGAATAAATCA
TGCCTACCTTTTCAACGGTAGGGATGGGGTTGGGAAAAAACTCCTGGCTTTTCAGTTTGCCAGGGCAATTAATTGCCATA
ATAAAGTAGATGACAGCTGTGGTCAGTGTATGGCCTGTAAAAAAATTGATCATTTTAATCATCCTGATTTAAAATTAATT
GAGCCGGTAAATAATAGTATTAAAATAGACCAGATAAGAAATTTCCAGAAAGAGATAGTTTATAAACCTTATGAAAGCAA
GAAAAAGATATATATAATAGACGGGGCCGATACCATGACTTTAGAAGCAGCCAATAGTCTATTAAAAACACTGGAAGAGC
CACCCCATTATGCAGTTATTATTTTACTGGTAGAAGATTTAAATAAACTGCCATCAACCATAATATCTAGGTGCCAGCAA
ATTCAGCTAAGCAATATTGCCCGGGAAATAATCAAGGAATATCTGATTAATAAAGGTATCGGGGATAAAAAGGCTCATTT
GATTGCTACACTGGCCAGGGGGAGCATTGGTGATGCCCTTAAATTAGTGGAAAAGGAAGATATTCTGTTAATCAGAGAAG
AATTGTTTGATTTTTTGTGTAATTTAACCCATCTCAGGGCAGTTGATATTTTCAATAAAAGCCAGCAAATGGTTGACTGG
TTTCAGCAAGATTTACCTCTGTTTGATTTGATATCTGGCTGGTTTAAAGATATAATATTTTATAATCAGGGAATTAAAGA
TAATATAGTAAATTATGATTTTATTGATAAAATAGAGGTTCAGGCCCGAAATTATAGAACTGAAGAGTTGATCTCTATTA
TTGAATTGATCGGAGAAACCAGGAAATATATAAAAGCAAATGTCAGGAAAGACCTGGCTTTACAGGTTATGTTATTGAAA
ATTAGAGCTAAGAGGGTGTAA

Upstream 100 bases:

>100_bases
TAAGTATTCATATAAAAGAAAAGTACTCATTTAAAAGAAACCAGGCTACAAAAGTTTCAATAGATAGAATATAGTTAATA
ATTACTAAGGGGTATCAAAT

Downstream 100 bases:

>100_bases
TAATTATGTATACAGTAATAGGAGTAAGCTTTAGACAAGCTGGAAAAATATATTATTTTGACCCGGGAGATATTGAACTT
AAAAAGGGTGATCATGTTAT

Product: DNA polymerase III, subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MGFNEIIGQNGAIKILKEELRDNRINHAYLFNGRDGVGKKLLAFQFARAINCHNKVDDSCGQCMACKKIDHFNHPDLKLI
EPVNNSIKIDQIRNFQKEIVYKPYESKKKIYIIDGADTMTLEAANSLLKTLEEPPHYAVIILLVEDLNKLPSTIISRCQQ
IQLSNIAREIIKEYLINKGIGDKKAHLIATLARGSIGDALKLVEKEDILLIREELFDFLCNLTHLRAVDIFNKSQQMVDW
FQQDLPLFDLISGWFKDIIFYNQGIKDNIVNYDFIDKIEVQARNYRTEELISIIELIGETRKYIKANVRKDLALQVMLLK
IRAKRV

Sequences:

>Translated_326_residues
MGFNEIIGQNGAIKILKEELRDNRINHAYLFNGRDGVGKKLLAFQFARAINCHNKVDDSCGQCMACKKIDHFNHPDLKLI
EPVNNSIKIDQIRNFQKEIVYKPYESKKKIYIIDGADTMTLEAANSLLKTLEEPPHYAVIILLVEDLNKLPSTIISRCQQ
IQLSNIAREIIKEYLINKGIGDKKAHLIATLARGSIGDALKLVEKEDILLIREELFDFLCNLTHLRAVDIFNKSQQMVDW
FQQDLPLFDLISGWFKDIIFYNQGIKDNIVNYDFIDKIEVQARNYRTEELISIIELIGETRKYIKANVRKDLALQVMLLK
IRAKRV
>Mature_325_residues
GFNEIIGQNGAIKILKEELRDNRINHAYLFNGRDGVGKKLLAFQFARAINCHNKVDDSCGQCMACKKIDHFNHPDLKLIE
PVNNSIKIDQIRNFQKEIVYKPYESKKKIYIIDGADTMTLEAANSLLKTLEEPPHYAVIILLVEDLNKLPSTIISRCQQI
QLSNIAREIIKEYLINKGIGDKKAHLIATLARGSIGDALKLVEKEDILLIREELFDFLCNLTHLRAVDIFNKSQQMVDWF
QQDLPLFDLISGWFKDIIFYNQGIKDNIVNYDFIDKIEVQARNYRTEELISIIELIGETRKYIKANVRKDLALQVMLLKI
RAKRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=210, Percent_Identity=34.2857142857143, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1787341, Length=189, Percent_Identity=26.984126984127, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37632; Mature: 37501

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFNEIIGQNGAIKILKEELRDNRINHAYLFNGRDGVGKKLLAFQFARAINCHNKVDDSC
CCCHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHH
GQCMACKKIDHFNHPDLKLIEPVNNSIKIDQIRNFQKEIVYKPYESKKKIYIIDGADTMT
HHHHHHHHHCCCCCCCCEEECCCCCCEEHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHH
LEAANSLLKTLEEPPHYAVIILLVEDLNKLPSTIISRCQQIQLSNIAREIIKEYLINKGI
HHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDKKAHLIATLARGSIGDALKLVEKEDILLIREELFDFLCNLTHLRAVDIFNKSQQMVDW
CCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FQQDLPLFDLISGWFKDIIFYNQGIKDNIVNYDFIDKIEVQARNYRTEELISIIELIGET
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
RKYIKANVRKDLALQVMLLKIRAKRV
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GFNEIIGQNGAIKILKEELRDNRINHAYLFNGRDGVGKKLLAFQFARAINCHNKVDDSC
CCHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHH
GQCMACKKIDHFNHPDLKLIEPVNNSIKIDQIRNFQKEIVYKPYESKKKIYIIDGADTMT
HHHHHHHHHCCCCCCCCEEECCCCCCEEHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHH
LEAANSLLKTLEEPPHYAVIILLVEDLNKLPSTIISRCQQIQLSNIAREIIKEYLINKGI
HHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDKKAHLIATLARGSIGDALKLVEKEDILLIREELFDFLCNLTHLRAVDIFNKSQQMVDW
CCCHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FQQDLPLFDLISGWFKDIIFYNQGIKDNIVNYDFIDKIEVQARNYRTEELISIIELIGET
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
RKYIKANVRKDLALQVMLLKIRAKRV
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]