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The map label for this gene is ydjE [H]

Identifier: 220932830

GI number: 220932830

Start: 2152552

End: 2153523

Strand: Reverse

Name: ydjE [H]

Synonym: Hore_19960

Alternate gene names: 220932830

Gene position: 2153523-2152552 (Counterclockwise)

Preceding gene: 220932831

Following gene: 220932829

Centisome position: 83.53

GC content: 42.9

Gene sequence:

>972_bases
GTGCCAGAAGTTATAACCATGGGAGAAGCTTTAATTGACTTTGTACCCCGCCAGGTAAATTGTGCCCTCCATGAGGTAAG
TGATTTTCACCGGGCGGCCGGTGGGGCGCCAGCTAATGTAGCGGTCGGGGTGGCCCGCCTGGGGGTGTCTGCCGGTTTTA
TGGGAAAGGTTGGTGATGATGCTTTTGGTTATTTTTTAAAAAAGACTCTTGAGGATAATAAGGTCAATACCTCCCAGATG
GTTTTGACTGAAGAAGCCATGACAACCCTGGCTTTTGTCTCCTTGAGGGGTGATGGGGAACGGGATTTTGCTTTTTACAG
GAAACCCGGGGCTGACATGCTTTACCGGGTTGAAGAGGTAGATTTTGACTATCTGGAGGGGAGTCATATATTCCATTTTG
GTTCTATTTCATTGATTACTGATCCGTCAAAAACGACTACCCTTAAACTTATAAAACAGGCCCGTAGTAAAGGGGTTACT
GTTTCCTTTGACCCTAATATTAGACCTCCTCTCTGGGGTTCTCTTGAGGAAGCGGTAAAACAAATTAATCAGGTTATACC
AGAGGCAGATATTCTAAAAATAAATGAAGAGGAGTTAAAGGTACTGACAGGGTTGAATGATTTAAAAGAGGAAAGTATAC
TTAAGGCCTGTCAGGAGTTTTATCAAAAGGGGCCTGAGCTAATAATTGTAACCCTGGGGGGAGAAGGCTGTTTTTATTAT
TCATCAGCTGGTTATGGAATGGTAGAAGGATTTAAGACAAAAGCTATAGATACTACCGGAGCCGGGGACAGTTTTGTGGC
TGCAGTATTAAGCAGTCTGGTGAAAGCTGATCTTACTAACCTACAAGGCTTAAGTACAAAAAAACTTGAAGAAATATTAA
CCTTTGCCAATAAAGTTGCCTCTATTGTTGTTACCAGGAATGGAGGAATTCCATCCCTTCCCTTTATTGATGAAGTTGAA
GGAGGTAGTTAA

Upstream 100 bases:

>100_bases
AGTATAAAAGGGAGGTAGTAATACTCCCTTTTATTTTGTTTATGATTGTTTCTATAGGTTATCTTTGATAAAAAATGATT
TATACCGGGGAGTGATTATT

Downstream 100 bases:

>100_bases
TTAATCAGACCTTCTTAATTACTGGATTATTACATGGGTTTACAAAATGACTGCTTCCAAACTGGCAGTCATTTTTTTAA
TTTAGGTTTTTTTCATAATT

Product: PfkB domain-containing protein

Products: ADP; D-fructose 6-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MPEVITMGEALIDFVPRQVNCALHEVSDFHRAAGGAPANVAVGVARLGVSAGFMGKVGDDAFGYFLKKTLEDNKVNTSQM
VLTEEAMTTLAFVSLRGDGERDFAFYRKPGADMLYRVEEVDFDYLEGSHIFHFGSISLITDPSKTTTLKLIKQARSKGVT
VSFDPNIRPPLWGSLEEAVKQINQVIPEADILKINEEELKVLTGLNDLKEESILKACQEFYQKGPELIIVTLGGEGCFYY
SSAGYGMVEGFKTKAIDTTGAGDSFVAAVLSSLVKADLTNLQGLSTKKLEEILTFANKVASIVVTRNGGIPSLPFIDEVE
GGS

Sequences:

>Translated_323_residues
MPEVITMGEALIDFVPRQVNCALHEVSDFHRAAGGAPANVAVGVARLGVSAGFMGKVGDDAFGYFLKKTLEDNKVNTSQM
VLTEEAMTTLAFVSLRGDGERDFAFYRKPGADMLYRVEEVDFDYLEGSHIFHFGSISLITDPSKTTTLKLIKQARSKGVT
VSFDPNIRPPLWGSLEEAVKQINQVIPEADILKINEEELKVLTGLNDLKEESILKACQEFYQKGPELIIVTLGGEGCFYY
SSAGYGMVEGFKTKAIDTTGAGDSFVAAVLSSLVKADLTNLQGLSTKKLEEILTFANKVASIVVTRNGGIPSLPFIDEVE
GGS
>Mature_322_residues
PEVITMGEALIDFVPRQVNCALHEVSDFHRAAGGAPANVAVGVARLGVSAGFMGKVGDDAFGYFLKKTLEDNKVNTSQMV
LTEEAMTTLAFVSLRGDGERDFAFYRKPGADMLYRVEEVDFDYLEGSHIFHFGSISLITDPSKTTTLKLIKQARSKGVTV
SFDPNIRPPLWGSLEEAVKQINQVIPEADILKINEEELKVLTGLNDLKEESILKACQEFYQKGPELIIVTLGGEGCFYYS
SAGYGMVEGFKTKAIDTTGAGDSFVAAVLSSLVKADLTNLQGLSTKKLEEILTFANKVASIVVTRNGGIPSLPFIDEVEG
GS

Specific function: Ribose metabolism; first step. [C]

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Homo sapiens, GI11545855, Length=285, Percent_Identity=29.4736842105263, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1790193, Length=294, Percent_Identity=25.1700680272109, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082281, Length=287, Percent_Identity=26.4808362369338, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082343, Length=299, Percent_Identity=29.4314381270903, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081971, Length=315, Percent_Identity=26.984126984127, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788417, Length=276, Percent_Identity=22.8260869565217, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17532905, Length=298, Percent_Identity=27.8523489932886, Blast_Score=91, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.4

Molecular weight: Translated: 34964; Mature: 34833

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEVITMGEALIDFVPRQVNCALHEVSDFHRAAGGAPANVAVGVARLGVSAGFMGKVGDD
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCH
AFGYFLKKTLEDNKVNTSQMVLTEEAMTTLAFVSLRGDGERDFAFYRKPGADMLYRVEEV
HHHHHHHHHHCCCCCCHHHEEHHHHHHHHHHEEEECCCCCCCCEEEECCCHHHHEEHHHC
DFDYLEGSHIFHFGSISLITDPSKTTTLKLIKQARSKGVTVSFDPNIRPPLWGSLEEAVK
CCCCCCCCEEEEECCEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHH
QINQVIPEADILKINEEELKVLTGLNDLKEESILKACQEFYQKGPELIIVTLGGEGCFYY
HHHHHCCCCCEEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEE
SSAGYGMVEGFKTKAIDTTGAGDSFVAAVLSSLVKADLTNLQGLSTKKLEEILTFANKVA
CCCCCCHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SIVVTRNGGIPSLPFIDEVEGGS
EEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PEVITMGEALIDFVPRQVNCALHEVSDFHRAAGGAPANVAVGVARLGVSAGFMGKVGDD
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCH
AFGYFLKKTLEDNKVNTSQMVLTEEAMTTLAFVSLRGDGERDFAFYRKPGADMLYRVEEV
HHHHHHHHHHCCCCCCHHHEEHHHHHHHHHHEEEECCCCCCCCEEEECCCHHHHEEHHHC
DFDYLEGSHIFHFGSISLITDPSKTTTLKLIKQARSKGVTVSFDPNIRPPLWGSLEEAVK
CCCCCCCCEEEEECCEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHH
QINQVIPEADILKINEEELKVLTGLNDLKEESILKACQEFYQKGPELIIVTLGGEGCFYY
HHHHHCCCCCEEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEE
SSAGYGMVEGFKTKAIDTTGAGDSFVAAVLSSLVKADLTNLQGLSTKKLEEILTFANKVA
CCCCCCHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SIVVTRNGGIPSLPFIDEVEGGS
EEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-fructose

Specific reaction: ATP + D-fructose = ADP + D-fructose 6-phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9455482; 9384377 [H]