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The map label for this gene is mdeA [H]

Identifier: 220932791

GI number: 220932791

Start: 2101720

End: 2102913

Strand: Reverse

Name: mdeA [H]

Synonym: Hore_19570

Alternate gene names: 220932791

Gene position: 2102913-2101720 (Counterclockwise)

Preceding gene: 220932792

Following gene: 220932790

Centisome position: 81.57

GC content: 47.74

Gene sequence:

>1194_bases
ATGAATAAAAAGACAAAAAAGCAGTATGGATTTAATACCCTGGCTTTACACCATGGTTATGACCCGGTCCAGGAAGGGAG
CAAATCCAGGGCAGTTCCCATTTACCAGACAACATCCTATATGTTTGATAGTGCTGAACATGCTGCTGGCCTGTTTGCCG
AAGAAGAAGAAGGGTATATTTATACCAGAATTGGGAACCCGACAACTAAAGTTTTTGAAGAAAGGATGGCCGTCCTCGAG
GGAGGGGAGGCCGGGCTGGCAACCTCATCGGGGCAGTCTGCTATTACCCTGACTATACTGACATTGGTCAGCCAGGGGGA
AGAGGTGGTATCATCAAGCTATATTTACGGAGGGACCTATCATCTTCTGGCTGAGAGTCTCCCCCGGTATGGGGTTAAGA
CCAGATTTGTCAAACCTGATGATATAAATGACTGGGAGCAGGCTATAACAGATAAAACCCGGGTTTTTTACCTGGAATCA
CCGGGCAATCCCCGGCTTAATATTGTTGATATTGAGGCTGTATCCAGCCTGGCCCATCAATACGGTATAACTGTAGTTGT
TGATAATACCTTTAATACTCCCTATTTAAGCCAGCCCCTTAAATTGGGGGCTGACATTGTAGTCCATTCTACTACCAAGT
ATATCGGGGGTCATGGTAATTCAATTGGGGGGGTTATTGTTGGAACCCGTGATTTTATCCATAAAGTCCGGACTGAGCTT
TACCGTGATACTGGTCCTGCCATAAGCCCCTTTAATGCCTGGCTTTTCATCCAGGGGTTAGAGACCCTTTCATTGAGAAT
GGAAAAACACTGTAGTAATGCCATGGAGGTTGCCCGGTGGCTCTCCGGAGATGAAAGGGTTGAATGGGTGACTTACCCTG
GCCTTCCTGACCATCCCCGGCATGAACTGGCCAAAAAGCAGCAGCGGGGGTTTGGTGGAATGATTTGTTTCGGGGTTAAA
GGTGGTTATTCAGCGGCCCGGAACCTTATCAACAGGGTGGAACTGTGTTCTCTACTTGCCAATATAGGTGATACCCGCAC
CCTTATTATTCACCCTGCCTCTACCACCCATGAGCAGTTGAGTAGAGAGGAGCAGGAAAAGGCAGGGGTTACCCCTGATT
TAATCAGACTATCGGTAGGAATAGAGGATGTATGGGATATAATTGATGACCTGGATCAGGCCCTGGGGGGGTAG

Upstream 100 bases:

>100_bases
TTTTGACAGAGGGTAATCGGGCAATATTATAGGGGGATTATGGTAAGGAATAAAAAGAGTTGTAACAGGATAATAAGGTA
ATTACAGGGAGGAATTATAA

Downstream 100 bases:

>100_bases
TTTATCTTTCTTTACTGTTTGTCTGGTTTTATTAAAAGCTTATTGTCTATCTGAAAGGTCAGAAGGGAGTAGATAGAATG
TCTTTCAGTGCCTTGAATGA

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNKKTKKQYGFNTLALHHGYDPVQEGSKSRAVPIYQTTSYMFDSAEHAAGLFAEEEEGYIYTRIGNPTTKVFEERMAVLE
GGEAGLATSSGQSAITLTILTLVSQGEEVVSSSYIYGGTYHLLAESLPRYGVKTRFVKPDDINDWEQAITDKTRVFYLES
PGNPRLNIVDIEAVSSLAHQYGITVVVDNTFNTPYLSQPLKLGADIVVHSTTKYIGGHGNSIGGVIVGTRDFIHKVRTEL
YRDTGPAISPFNAWLFIQGLETLSLRMEKHCSNAMEVARWLSGDERVEWVTYPGLPDHPRHELAKKQQRGFGGMICFGVK
GGYSAARNLINRVELCSLLANIGDTRTLIIHPASTTHEQLSREEQEKAGVTPDLIRLSVGIEDVWDIIDDLDQALGG

Sequences:

>Translated_397_residues
MNKKTKKQYGFNTLALHHGYDPVQEGSKSRAVPIYQTTSYMFDSAEHAAGLFAEEEEGYIYTRIGNPTTKVFEERMAVLE
GGEAGLATSSGQSAITLTILTLVSQGEEVVSSSYIYGGTYHLLAESLPRYGVKTRFVKPDDINDWEQAITDKTRVFYLES
PGNPRLNIVDIEAVSSLAHQYGITVVVDNTFNTPYLSQPLKLGADIVVHSTTKYIGGHGNSIGGVIVGTRDFIHKVRTEL
YRDTGPAISPFNAWLFIQGLETLSLRMEKHCSNAMEVARWLSGDERVEWVTYPGLPDHPRHELAKKQQRGFGGMICFGVK
GGYSAARNLINRVELCSLLANIGDTRTLIIHPASTTHEQLSREEQEKAGVTPDLIRLSVGIEDVWDIIDDLDQALGG
>Mature_397_residues
MNKKTKKQYGFNTLALHHGYDPVQEGSKSRAVPIYQTTSYMFDSAEHAAGLFAEEEEGYIYTRIGNPTTKVFEERMAVLE
GGEAGLATSSGQSAITLTILTLVSQGEEVVSSSYIYGGTYHLLAESLPRYGVKTRFVKPDDINDWEQAITDKTRVFYLES
PGNPRLNIVDIEAVSSLAHQYGITVVVDNTFNTPYLSQPLKLGADIVVHSTTKYIGGHGNSIGGVIVGTRDFIHKVRTEL
YRDTGPAISPFNAWLFIQGLETLSLRMEKHCSNAMEVARWLSGDERVEWVTYPGLPDHPRHELAKKQQRGFGGMICFGVK
GGYSAARNLINRVELCSLLANIGDTRTLIIHPASTTHEQLSREEQEKAGVTPDLIRLSVGIEDVWDIIDDLDQALGG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=386, Percent_Identity=38.3419689119171, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI299473759, Length=386, Percent_Identity=35.2331606217617, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI25453487, Length=385, Percent_Identity=33.7662337662338, Blast_Score=192, Evalue=6e-49,
Organism=Escherichia coli, GI1790375, Length=365, Percent_Identity=35.6164383561644, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1789383, Length=366, Percent_Identity=27.8688524590164, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17538055, Length=388, Percent_Identity=37.8865979381443, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=39.0835579514825, Blast_Score=249, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17560056, Length=388, Percent_Identity=38.4020618556701, Blast_Score=249, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=44.4444444444444, Blast_Score=335, Evalue=7e-93,
Organism=Saccharomyces cerevisiae, GI6319307, Length=392, Percent_Identity=39.030612244898, Blast_Score=250, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6321254, Length=390, Percent_Identity=28.974358974359, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6321170, Length=273, Percent_Identity=30.03663003663, Blast_Score=139, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=25.6345177664975, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922554, Length=388, Percent_Identity=37.3711340206186, Blast_Score=261, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43855; Mature: 43855

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKTKKQYGFNTLALHHGYDPVQEGSKSRAVPIYQTTSYMFDSAEHAAGLFAEEEEGYI
CCCCCCHHCCCEEEEEECCCCHHHCCCCCCCEEEEECHHHHHCCHHHHHCCCEECCCCEE
YTRIGNPTTKVFEERMAVLEGGEAGLATSSGQSAITLTILTLVSQGEEVVSSSYIYGGTY
EEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHCCHHHHHCCEEECCHH
HLLAESLPRYGVKTRFVKPDDINDWEQAITDKTRVFYLESPGNPRLNIVDIEAVSSLAHQ
HHHHHHCCCCCCEEEECCCCCCCHHHHHHCCCEEEEEEECCCCCCEEEEEHHHHHHHHHH
YGITVVVDNTFNTPYLSQPLKLGADIVVHSTTKYIGGHGNSIGGVIVGTRDFIHKVRTEL
CCCEEEEECCCCCCCCCCHHHCCCEEEEEECHHHCCCCCCCCCEEEECCHHHHHHHHHHH
YRDTGPAISPFNAWLFIQGLETLSLRMEKHCSNAMEVARWLSGDERVEWVTYPGLPDHPR
HHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCH
HELAKKQQRGFGGMICFGVKGGYSAARNLINRVELCSLLANIGDTRTLIIHPASTTHEQL
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHH
SREEQEKAGVTPDLIRLSVGIEDVWDIIDDLDQALGG
HHHHHHHCCCCHHHEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKKTKKQYGFNTLALHHGYDPVQEGSKSRAVPIYQTTSYMFDSAEHAAGLFAEEEEGYI
CCCCCCHHCCCEEEEEECCCCHHHCCCCCCCEEEEECHHHHHCCHHHHHCCCEECCCCEE
YTRIGNPTTKVFEERMAVLEGGEAGLATSSGQSAITLTILTLVSQGEEVVSSSYIYGGTY
EEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHCCHHHHHCCEEECCHH
HLLAESLPRYGVKTRFVKPDDINDWEQAITDKTRVFYLESPGNPRLNIVDIEAVSSLAHQ
HHHHHHCCCCCCEEEECCCCCCCHHHHHHCCCEEEEEEECCCCCCEEEEEHHHHHHHHHH
YGITVVVDNTFNTPYLSQPLKLGADIVVHSTTKYIGGHGNSIGGVIVGTRDFIHKVRTEL
CCCEEEEECCCCCCCCCCHHHCCCEEEEEECHHHCCCCCCCCCEEEECCHHHHHHHHHHH
YRDTGPAISPFNAWLFIQGLETLSLRMEKHCSNAMEVARWLSGDERVEWVTYPGLPDHPR
HHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCH
HELAKKQQRGFGGMICFGVKGGYSAARNLINRVELCSLLANIGDTRTLIIHPASTTHEQL
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHH
SREEQEKAGVTPDLIRLSVGIEDVWDIIDDLDQALGG
HHHHHHHCCCCHHHEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]