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The map label for this gene is spsK [H]

Identifier: 220932571

GI number: 220932571

Start: 1853728

End: 1854591

Strand: Reverse

Name: spsK [H]

Synonym: Hore_17350

Alternate gene names: 220932571

Gene position: 1854591-1853728 (Counterclockwise)

Preceding gene: 220932572

Following gene: 220932570

Centisome position: 71.94

GC content: 39.35

Gene sequence:

>864_bases
TTGAAAGTATTGGTGACCGGAGACAAGAAATTCTGCAGGACTTTTGTTAAGAAACTGGGTACCAGATACCGGGTTATTTT
TTTAGGAAGAGATGACCTTGATATTACAAATACCCTGGAAGTGGAAAAAAAGATACTTGATGTCTATCCTGATATAGTTA
TTCACTGTCTGGCCCTGGATGATATTGAGGCCTGTGAACGTGATAAAGCAATGGCCTTTCAGATTAATTCTTACGGTGCC
CAGAATGTTGCCATTGCTTCCAGTATGTGTGAGGCTAAACTTGTTTACATAAGCACCAGTCATGTTTTCAGTGGCAGGCT
TAATAGACCTTACCGGGAGTCAGATGAGCTTCAACCGGTAAATGTTTACGGTTTATCTAAACTACTTGCTGAAAATTTTA
TTAAAGAAATAAACAGGAAATTTTTTATTGTAAGGACTGGCTGGCTTTACGGGCCGGGCCGGGATTCTCTTATAGGCAAA
TTGATTGAAACTTCAAAGCAGGAAAAAACAGTTGAGCTTCCCGGTGACAGATATGATACTCCTACCTTTATTGGAGATCT
GGCCCATATTATTGGACGGTTAATGACTACTGATTTTTATGGTACCTATCATATAACAAATAGTGGCTACTGTAGCCTGT
ATAGCTGGGGCCTGGAAGTAGTCAGGGCATTGAAATTAAATGTAAATATTAAAGAGGTAACCCTGGAACACCAGTCTGGT
CTGGTTAGAATACCGGAATATACAGTTCTTGATAATAACCTGGTAGCTAATAAACTTGGTATCAGGCCCAGGGGCTGGAG
GAAGGCCCTGAATTCTTACCTGGTTGATTATTTAATATATAATTCTATAAACAGTGTATCTTAA

Upstream 100 bases:

>100_bases
TGAATTATTTTATGATGGCAGTAAAAAGATGGGATAAATTCACCAAATTCCCCCCGGTATCATAAGATAATTTTAGCATG
GGTATTAGGGGGGACTGGTT

Downstream 100 bases:

>100_bases
CCATATCTTCATCAGTTTTAAATCAGGGTCTTTTATTATTGATTTTTTTGTGGGCAAAACCGGGCTATGACTACAATACA
ATTTTATATTATAAAATTTT

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKVLVTGDKKFCRTFVKKLGTRYRVIFLGRDDLDITNTLEVEKKILDVYPDIVIHCLALDDIEACERDKAMAFQINSYGA
QNVAIASSMCEAKLVYISTSHVFSGRLNRPYRESDELQPVNVYGLSKLLAENFIKEINRKFFIVRTGWLYGPGRDSLIGK
LIETSKQEKTVELPGDRYDTPTFIGDLAHIIGRLMTTDFYGTYHITNSGYCSLYSWGLEVVRALKLNVNIKEVTLEHQSG
LVRIPEYTVLDNNLVANKLGIRPRGWRKALNSYLVDYLIYNSINSVS

Sequences:

>Translated_287_residues
MKVLVTGDKKFCRTFVKKLGTRYRVIFLGRDDLDITNTLEVEKKILDVYPDIVIHCLALDDIEACERDKAMAFQINSYGA
QNVAIASSMCEAKLVYISTSHVFSGRLNRPYRESDELQPVNVYGLSKLLAENFIKEINRKFFIVRTGWLYGPGRDSLIGK
LIETSKQEKTVELPGDRYDTPTFIGDLAHIIGRLMTTDFYGTYHITNSGYCSLYSWGLEVVRALKLNVNIKEVTLEHQSG
LVRIPEYTVLDNNLVANKLGIRPRGWRKALNSYLVDYLIYNSINSVS
>Mature_287_residues
MKVLVTGDKKFCRTFVKKLGTRYRVIFLGRDDLDITNTLEVEKKILDVYPDIVIHCLALDDIEACERDKAMAFQINSYGA
QNVAIASSMCEAKLVYISTSHVFSGRLNRPYRESDELQPVNVYGLSKLLAENFIKEINRKFFIVRTGWLYGPGRDSLIGK
LIETSKQEKTVELPGDRYDTPTFIGDLAHIIGRLMTTDFYGTYHITNSGYCSLYSWGLEVVRALKLNVNIKEVTLEHQSG
LVRIPEYTVLDNNLVANKLGIRPRGWRKALNSYLVDYLIYNSINSVS

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=301, Percent_Identity=25.2491694352159, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI33519455, Length=301, Percent_Identity=25.2491694352159, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1788352, Length=189, Percent_Identity=28.042328042328, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32696; Mature: 32696

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVTGDKKFCRTFVKKLGTRYRVIFLGRDDLDITNTLEVEKKILDVYPDIVIHCLALD
CEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEHHHCC
DIEACERDKAMAFQINSYGAQNVAIASSMCEAKLVYISTSHVFSGRLNRPYRESDELQPV
CHHHHCCCCEEEEEEECCCCCHHHHHHHHCCEEEEEEEECCEECCCCCCCCCCCCCCCCC
NVYGLSKLLAENFIKEINRKFFIVRTGWLYGPGRDSLIGKLIETSKQEKTVELPGDRYDT
HHHHHHHHHHHHHHHHCCCEEEEEEECEEECCCHHHHHHHHHHCCCCCCEEECCCCCCCC
PTFIGDLAHIIGRLMTTDFYGTYHITNSGYCSLYSWGLEVVRALKLNVNIKEVTLEHQSG
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEHEEEEECCCC
LVRIPEYTVLDNNLVANKLGIRPRGWRKALNSYLVDYLIYNSINSVS
EEECCCEEEECCCEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVLVTGDKKFCRTFVKKLGTRYRVIFLGRDDLDITNTLEVEKKILDVYPDIVIHCLALD
CEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEHHHCC
DIEACERDKAMAFQINSYGAQNVAIASSMCEAKLVYISTSHVFSGRLNRPYRESDELQPV
CHHHHCCCCEEEEEEECCCCCHHHHHHHHCCEEEEEEEECCEECCCCCCCCCCCCCCCCC
NVYGLSKLLAENFIKEINRKFFIVRTGWLYGPGRDSLIGKLIETSKQEKTVELPGDRYDT
HHHHHHHHHHHHHHHHCCCEEEEEEECEEECCCHHHHHHHHHHCCCCCCEEECCCCCCCC
PTFIGDLAHIIGRLMTTDFYGTYHITNSGYCSLYSWGLEVVRALKLNVNIKEVTLEHQSG
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEHEEEEECCCC
LVRIPEYTVLDNNLVANKLGIRPRGWRKALNSYLVDYLIYNSINSVS
EEECCCEEEECCCEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]