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The map label for this gene is rfaQ [H]

Identifier: 220932556

GI number: 220932556

Start: 1836032

End: 1837114

Strand: Reverse

Name: rfaQ [H]

Synonym: Hore_17200

Alternate gene names: 220932556

Gene position: 1837114-1836032 (Counterclockwise)

Preceding gene: 220932557

Following gene: 220932555

Centisome position: 71.26

GC content: 44.6

Gene sequence:

>1083_bases
GTGAAGATCCTTGTTTTAATTCTATCAGCTCTGGGTGATGCCCTGTTTACGACACCGGCACTCAGGGCTTTAAGGAACGG
TTATCCGGATGCCAGAATAGATGCCCTGGTCTGGGAAGATAATAAAACTATTCTGGAAGGCAACCCCAACCTGGATAGGC
TGATAGTCAGTAAAGGGAAAGGAAAGCTTATCTCCGATGTTCAGAGCTTAAGGGAGGAGGTTTACGACCTGGCCATTGGA
TTATCCAATGCAGGCAGTTATATAGCATATCTGGTTAATGCCAGAAAAAGGCTGGGTTTCAAGGGTTCTGAAATAGGGTG
GATGTATGACTTCAATGTACCCGATGATAGAAACATCCATGCCGTCGATTACTGCCTCGAGATTGTAAAGGTGGCAGGGG
GTAAACCCGATATAAATCCTCACCTCGAGGTTGCAATAAATGACGAACAACGTCGGTCTGTGGCTGAATTTATGAAAAAA
AAGGGTATTATAACTTCTTTACCCCTGGTGGCTATTCACCCTGGAGGTAAATATTTCTCCTTAAAGCGCTGGCCAGTGGA
TAAATTTTCTGAGTTGGTTAAGGTCCTGGATAAAATGATACCCCTTCAGGTTGTTCTGGTCGGAGGGCCTGATGATAGAG
AACTTGCCACCCGGATTATTGACCCTGATTATAATTATAACCGTAAACCTGTAATTGTTGCCGGAGAGTTATCGGTTAAG
GAAACTACGGCCCTGCTGGAACAGGTTGATTTGTTTATTGGAAATGATTCTGCTCCCCAGCATATGGCCTCAGCTGTGGG
GGTACCGGTGGTTTCTCTGTTTGGCCCTACCAATCCGGCAAACTTTTATCCATATGGGACTGAACACATTATAATTCGCT
CTGACCTCTCCTGTAGCCCCTGTTTTTCCTGGTTGGGGGATTTAAAACAATACCTGCCTGAATATTTACCAGAATGGGTA
ACTAAATGTAAGGGTAAGTGTATGGAGGAAATAGAGGTAGCCGATGTTGTCAGAGCGGCCCGGCAGTTATTAGTTAACAA
AAGGTCAGGTTATAAACTTATTTCAACTGGGGGTAGTGTCTAA

Upstream 100 bases:

>100_bases
AATAGTGTTAGGGAGATATAGAGCACGTTAGTGAGTTATAAAGTACCAGTAAAACATAAATCAGGGGTACCAGGATTTAT
CCAGAAAGCGGGGTGAAAAT

Downstream 100 bases:

>100_bases
ATGAAAATCGGTTTTTTTACTGACAGCTATAAACCATATATGAGTGGTGTGGTTAAGTCAATTGAGACCTTTACAGAACA
GCTACAGAAAAAGGGACATG

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKILVLILSALGDALFTTPALRALRNGYPDARIDALVWEDNKTILEGNPNLDRLIVSKGKGKLISDVQSLREEVYDLAIG
LSNAGSYIAYLVNARKRLGFKGSEIGWMYDFNVPDDRNIHAVDYCLEIVKVAGGKPDINPHLEVAINDEQRRSVAEFMKK
KGIITSLPLVAIHPGGKYFSLKRWPVDKFSELVKVLDKMIPLQVVLVGGPDDRELATRIIDPDYNYNRKPVIVAGELSVK
ETTALLEQVDLFIGNDSAPQHMASAVGVPVVSLFGPTNPANFYPYGTEHIIIRSDLSCSPCFSWLGDLKQYLPEYLPEWV
TKCKGKCMEEIEVADVVRAARQLLVNKRSGYKLISTGGSV

Sequences:

>Translated_360_residues
MKILVLILSALGDALFTTPALRALRNGYPDARIDALVWEDNKTILEGNPNLDRLIVSKGKGKLISDVQSLREEVYDLAIG
LSNAGSYIAYLVNARKRLGFKGSEIGWMYDFNVPDDRNIHAVDYCLEIVKVAGGKPDINPHLEVAINDEQRRSVAEFMKK
KGIITSLPLVAIHPGGKYFSLKRWPVDKFSELVKVLDKMIPLQVVLVGGPDDRELATRIIDPDYNYNRKPVIVAGELSVK
ETTALLEQVDLFIGNDSAPQHMASAVGVPVVSLFGPTNPANFYPYGTEHIIIRSDLSCSPCFSWLGDLKQYLPEYLPEWV
TKCKGKCMEEIEVADVVRAARQLLVNKRSGYKLISTGGSV
>Mature_360_residues
MKILVLILSALGDALFTTPALRALRNGYPDARIDALVWEDNKTILEGNPNLDRLIVSKGKGKLISDVQSLREEVYDLAIG
LSNAGSYIAYLVNARKRLGFKGSEIGWMYDFNVPDDRNIHAVDYCLEIVKVAGGKPDINPHLEVAINDEQRRSVAEFMKK
KGIITSLPLVAIHPGGKYFSLKRWPVDKFSELVKVLDKMIPLQVVLVGGPDDRELATRIIDPDYNYNRKPVIVAGELSVK
ETTALLEQVDLFIGNDSAPQHMASAVGVPVVSLFGPTNPANFYPYGTEHIIIRSDLSCSPCFSWLGDLKQYLPEYLPEWV
TKCKGKCMEEIEVADVVRAARQLLVNKRSGYKLISTGGSV

Specific function: Catalyzes heptose transfer to the lipopolysaccharide core. It transfers a heptose, called heptose(III), to the heptose(II) of the inner core [H]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790062, Length=297, Percent_Identity=32.3232323232323, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790050, Length=315, Percent_Identity=28.2539682539683, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011916 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 39902; Mature: 39902

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVLILSALGDALFTTPALRALRNGYPDARIDALVWEDNKTILEGNPNLDRLIVSKGK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEECCCCCCCEEEECCC
GKLISDVQSLREEVYDLAIGLSNAGSYIAYLVNARKRLGFKGSEIGWMYDFNVPDDRNIH
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCH
AVDYCLEIVKVAGGKPDINPHLEVAINDEQRRSVAEFMKKKGIITSLPLVAIHPGGKYFS
HHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEECCEEEECCCCCEEE
LKRWPVDKFSELVKVLDKMIPLQVVLVGGPDDRELATRIIDPDYNYNRKPVIVAGELSVK
EECCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCEEEECCCCHH
ETTALLEQVDLFIGNDSAPQHMASAVGVPVVSLFGPTNPANFYPYGTEHIIIRSDLSCSP
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHH
CFSWLGDLKQYLPEYLPEWVTKCKGKCMEEIEVADVVRAARQLLVNKRSGYKLISTGGSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
>Mature Secondary Structure
MKILVLILSALGDALFTTPALRALRNGYPDARIDALVWEDNKTILEGNPNLDRLIVSKGK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEECCCCCCCEEEECCC
GKLISDVQSLREEVYDLAIGLSNAGSYIAYLVNARKRLGFKGSEIGWMYDFNVPDDRNIH
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCH
AVDYCLEIVKVAGGKPDINPHLEVAINDEQRRSVAEFMKKKGIITSLPLVAIHPGGKYFS
HHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEECCEEEECCCCCEEE
LKRWPVDKFSELVKVLDKMIPLQVVLVGGPDDRELATRIIDPDYNYNRKPVIVAGELSVK
EECCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCEEEECCCCHH
ETTALLEQVDLFIGNDSAPQHMASAVGVPVVSLFGPTNPANFYPYGTEHIIIRSDLSCSP
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHH
CFSWLGDLKQYLPEYLPEWVTKCKGKCMEEIEVADVVRAARQLLVNKRSGYKLISTGGSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1732225; 1447141; 8041620; 9278503 [H]