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The map label for this gene is iclR [H]

Identifier: 220932468

GI number: 220932468

Start: 1754569

End: 1755336

Strand: Reverse

Name: iclR [H]

Synonym: Hore_16320

Alternate gene names: 220932468

Gene position: 1755336-1754569 (Counterclockwise)

Preceding gene: 220932469

Following gene: 220932467

Centisome position: 68.09

GC content: 43.1

Gene sequence:

>768_bases
TTGAATAGAGGTACTGTAGTCCGTTCCGTGGTAAAAGCCGTTAAAATATATGAAGAACTGGTTAAAGCGGGTAGCCCTTT
GCCCCTCAGCACCCTGAGCAAGAGGGTGGGGATAAATATCAGTACTGTCCACAGGCTGGTAAATACCCTGGTTGATATGG
GGTATGTTGAACAGGATGAAGACGGAAATTATGGCCTCGGGCTCCATTCGTTTGAAATGGCCAATATTATTACCCGTAAA
TTTGATATTAAAAAACTGGTAAGACCCTATTTAAAAGAAATTGTCGAAAGCTGTAATGAGACCTGTAATCTGGTAGTGAT
GGAGAATTATGAAGTGGTTTATTTAGACCAGGTTGAATCTACCAATATGGTCAGGATGTTTGCCAGTGTAGGGAGTAGAG
GGCCAGCCTACTGTACCGGTTCTGGCAAAGCATTGCTGGCCTATCTGGATGAAGATGAAATTGATTACTATCTCAAAGTA
ACTGACTTTAAAAGGTATACTGATAATACCATTACTGAACCCGATGAATTAAAAAAAGAACTGGCTAATATAAGAGAACA
GGGTTATGCCCTTGATCTCGAGGAGATGGAGCTGGGGGTAAGATGTGTGGCCGCTCCCTTTTTTGGTGGTGATGGTGATA
AGCTTCTGGGGGCTATCAGTGTTTCCGGGCCCTGTACCCGGATAACAATGGATTATCTGGAAGATGTCCTGATCCCTCTG
GTAACCGAAAAAGCAGGGCAACTTACTGAAAAACTTACCGTTGCTTAA

Upstream 100 bases:

>100_bases
ATAATTAAAAAAATACTGCCTTATGGTAGTATTTTTTTACCCGATTTTAGTTTCATATAACGAAAAATCATTTTACATAA
TGAAAAAAGGGGTGATTTTT

Downstream 100 bases:

>100_bases
ACCAGGAGTTCAATTTTTAATGGTCTCCTTTTAGAAAATACTGCAGGTGAGGTGATTAAAATGATGCAGGGAAGTCAACC
CGGAGAAAAAATAATGTCAG

Product: Transcriptional regulator IclR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNRGTVVRSVVKAVKIYEELVKAGSPLPLSTLSKRVGINISTVHRLVNTLVDMGYVEQDEDGNYGLGLHSFEMANIITRK
FDIKKLVRPYLKEIVESCNETCNLVVMENYEVVYLDQVESTNMVRMFASVGSRGPAYCTGSGKALLAYLDEDEIDYYLKV
TDFKRYTDNTITEPDELKKELANIREQGYALDLEEMELGVRCVAAPFFGGDGDKLLGAISVSGPCTRITMDYLEDVLIPL
VTEKAGQLTEKLTVA

Sequences:

>Translated_255_residues
MNRGTVVRSVVKAVKIYEELVKAGSPLPLSTLSKRVGINISTVHRLVNTLVDMGYVEQDEDGNYGLGLHSFEMANIITRK
FDIKKLVRPYLKEIVESCNETCNLVVMENYEVVYLDQVESTNMVRMFASVGSRGPAYCTGSGKALLAYLDEDEIDYYLKV
TDFKRYTDNTITEPDELKKELANIREQGYALDLEEMELGVRCVAAPFFGGDGDKLLGAISVSGPCTRITMDYLEDVLIPL
VTEKAGQLTEKLTVA
>Mature_255_residues
MNRGTVVRSVVKAVKIYEELVKAGSPLPLSTLSKRVGINISTVHRLVNTLVDMGYVEQDEDGNYGLGLHSFEMANIITRK
FDIKKLVRPYLKEIVESCNETCNLVVMENYEVVYLDQVESTNMVRMFASVGSRGPAYCTGSGKALLAYLDEDEIDYYLKV
TDFKRYTDNTITEPDELKKELANIREQGYALDLEEMELGVRCVAAPFFGGDGDKLLGAISVSGPCTRITMDYLEDVLIPL
VTEKAGQLTEKLTVA

Specific function: Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyr

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082359, Length=228, Percent_Identity=34.6491228070175, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1786468, Length=224, Percent_Identity=32.1428571428571, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1788131, Length=237, Percent_Identity=33.7552742616034, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786715, Length=221, Percent_Identity=32.1266968325792, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1789999, Length=237, Percent_Identity=32.0675105485232, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788581, Length=232, Percent_Identity=30.1724137931034, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1790752, Length=216, Percent_Identity=30.0925925925926, Blast_Score=100, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28389; Mature: 28389

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRGTVVRSVVKAVKIYEELVKAGSPLPLSTLSKRVGINISTVHRLVNTLVDMGYVEQDE
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
DGNYGLGLHSFEMANIITRKFDIKKLVRPYLKEIVESCNETCNLVVMENYEVVYLDQVES
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEEEECCEEEEEECCCC
TNMVRMFASVGSRGPAYCTGSGKALLAYLDEDEIDYYLKVTDFKRYTDNTITEPDELKKE
CHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCEEEEEEECCHHHCCCCCCCHHHHHHH
LANIREQGYALDLEEMELGVRCVAAPFFGGDGDKLLGAISVSGPCTRITMDYLEDVLIPL
HHHHHHCCCCCCHHHHHHCEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
VTEKAGQLTEKLTVA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRGTVVRSVVKAVKIYEELVKAGSPLPLSTLSKRVGINISTVHRLVNTLVDMGYVEQDE
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
DGNYGLGLHSFEMANIITRKFDIKKLVRPYLKEIVESCNETCNLVVMENYEVVYLDQVES
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEEEECCEEEEEECCCC
TNMVRMFASVGSRGPAYCTGSGKALLAYLDEDEIDYYLKVTDFKRYTDNTITEPDELKKE
CHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCEEEEEEECCHHHCCCCCCCHHHHHHH
LANIREQGYALDLEEMELGVRCVAAPFFGGDGDKLLGAISVSGPCTRITMDYLEDVLIPL
HHHHHHCCCCCCHHHHHHCEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
VTEKAGQLTEKLTVA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185227; 1995431; 8265357; 9278503; 1995429 [H]