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The map label for this gene is glyA

Identifier: 220932449

GI number: 220932449

Start: 1728695

End: 1729933

Strand: Reverse

Name: glyA

Synonym: Hore_16130

Alternate gene names: 220932449

Gene position: 1729933-1728695 (Counterclockwise)

Preceding gene: 220932450

Following gene: 220932448

Centisome position: 67.1

GC content: 44.63

Gene sequence:

>1239_bases
ATGGAGTTTTTAAAAAAAGTTGACCCGGATATTTTCGGGTTAATCGAGGAAGAGGATCAACGACAGCGCCGGAACATTGA
ACTTATTGCTTCAGAAAATTTTGTAAGTGATGCCGTTATGGAGGCAGCCGGTTCCTGCTTAACCAATAAGTATGCAGAAG
GTTATCCCCATAAGAGGTATTACGGGGGATGTGAGGTAGTCGATAAGGTTGAGGAACTGGCAATAGCCAGGGCCAGGGAG
TTATTCGGAGCGGAGCATGTTAATGTCCAGCCCCACTCAGGGTCTCAGGCCAATCAGGCTGTTTATTTCGCTACTGTACC
TCCCGGAGGGACTATACTGGCCATGGATTTAACCCATGGGGGCCATCTTACCCATGGTAGTCCTGTAAATATGTCCGGCA
AGTATTATAATTTCATCCATTATGGAGTTACCAGAGAAGAGGAGGTAATTGACTTTGATCAGGTCCGGGAACTGGCCAGA
AAGCACCAACCTGATTTAATTGTGGCCGGGGCCAGTGCCTATCCCAGGATAATTGATTTTGAAGTCTTCAGGGAGATTGC
CGATGAGGTTGGGGCTCTGTTTATGGTGGATATGGCCCATATTGCTGGCCTGGTGGCAGCGGGCTTACATCCGAGTCCAG
TACCGGTAGCCGATTTTGTGACAACAACAACCCATAAAACACTGAGGGGAACCAGAGGGGGATTAATTCTCTGTAAAGGG
GAACACGCTAAAAAAATAGATAAGGCTATATTCCCGGGATTACAGGGAGGCCCTCTTCTTCATATTATTGCGGCCAAGGC
AGTAACGTTCAAAGAAGCTCTACAGGATGAATTTAAAGGCTATCAGAAACAGATTGTGAGTAATGCTAAAACTATGGCAG
CTGAATTGAAAAATTATGGGTTCAGACTGGTTTCAGGTGGTACCGATAATCATCTGATGCTGGTTGATCTCACCAATATG
GACATAACTGGAAAGGATGCTGAAACTGCCCTTGATAAAGTGGGTATCACTGTTAATAAAAATACAATACCCTTTGAAAA
GAGGAGTCCCTTTGTTACCAGTGGTATTAGAATTGGGACTCCGGCCGTAACCACCAGGGGTATGAAAGAAAAAGAAATGA
AGTTGATTGCAAAGTTAATATACCAGACCCTTAAGAATATAAATGATGAAGGAAGGCTGACCGAAATCAGGCAAGAAGTT
TACAGGCTTTCTGAAAGGTTTCCAAAGACAGCGGAATAA

Upstream 100 bases:

>100_bases
TTTATATTAACCAGATATATTTTGGGTTACAGGAGGAAAAGTCATAAGGCCAATGTAAAAGGGAGTTAATGTAAAAACCT
TAAAAGGGAGTGATAAAATA

Downstream 100 bases:

>100_bases
CATTATAAGTTACAATTACAATTAATACGAAACGGGAGAGACAGGTAATGAAAGAATATAGAATGGTAGCTATGGATCTG
GATGGAACCCTGATTGATGA

Product: glycine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MEFLKKVDPDIFGLIEEEDQRQRRNIELIASENFVSDAVMEAAGSCLTNKYAEGYPHKRYYGGCEVVDKVEELAIARARE
LFGAEHVNVQPHSGSQANQAVYFATVPPGGTILAMDLTHGGHLTHGSPVNMSGKYYNFIHYGVTREEEVIDFDQVRELAR
KHQPDLIVAGASAYPRIIDFEVFREIADEVGALFMVDMAHIAGLVAAGLHPSPVPVADFVTTTTHKTLRGTRGGLILCKG
EHAKKIDKAIFPGLQGGPLLHIIAAKAVTFKEALQDEFKGYQKQIVSNAKTMAAELKNYGFRLVSGGTDNHLMLVDLTNM
DITGKDAETALDKVGITVNKNTIPFEKRSPFVTSGIRIGTPAVTTRGMKEKEMKLIAKLIYQTLKNINDEGRLTEIRQEV
YRLSERFPKTAE

Sequences:

>Translated_412_residues
MEFLKKVDPDIFGLIEEEDQRQRRNIELIASENFVSDAVMEAAGSCLTNKYAEGYPHKRYYGGCEVVDKVEELAIARARE
LFGAEHVNVQPHSGSQANQAVYFATVPPGGTILAMDLTHGGHLTHGSPVNMSGKYYNFIHYGVTREEEVIDFDQVRELAR
KHQPDLIVAGASAYPRIIDFEVFREIADEVGALFMVDMAHIAGLVAAGLHPSPVPVADFVTTTTHKTLRGTRGGLILCKG
EHAKKIDKAIFPGLQGGPLLHIIAAKAVTFKEALQDEFKGYQKQIVSNAKTMAAELKNYGFRLVSGGTDNHLMLVDLTNM
DITGKDAETALDKVGITVNKNTIPFEKRSPFVTSGIRIGTPAVTTRGMKEKEMKLIAKLIYQTLKNINDEGRLTEIRQEV
YRLSERFPKTAE
>Mature_412_residues
MEFLKKVDPDIFGLIEEEDQRQRRNIELIASENFVSDAVMEAAGSCLTNKYAEGYPHKRYYGGCEVVDKVEELAIARARE
LFGAEHVNVQPHSGSQANQAVYFATVPPGGTILAMDLTHGGHLTHGSPVNMSGKYYNFIHYGVTREEEVIDFDQVRELAR
KHQPDLIVAGASAYPRIIDFEVFREIADEVGALFMVDMAHIAGLVAAGLHPSPVPVADFVTTTTHKTLRGTRGGLILCKG
EHAKKIDKAIFPGLQGGPLLHIIAAKAVTFKEALQDEFKGYQKQIVSNAKTMAAELKNYGFRLVSGGTDNHLMLVDLTNM
DITGKDAETALDKVGITVNKNTIPFEKRSPFVTSGIRIGTPAVTTRGMKEKEMKLIAKLIYQTLKNINDEGRLTEIRQEV
YRLSERFPKTAE

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=452, Percent_Identity=44.4690265486726, Blast_Score=351, Evalue=6e-97,
Organism=Homo sapiens, GI261862350, Length=452, Percent_Identity=44.4690265486726, Blast_Score=351, Evalue=6e-97,
Organism=Homo sapiens, GI261862348, Length=452, Percent_Identity=44.4690265486726, Blast_Score=351, Evalue=6e-97,
Organism=Homo sapiens, GI19923315, Length=452, Percent_Identity=44.4690265486726, Blast_Score=351, Evalue=7e-97,
Organism=Homo sapiens, GI261862346, Length=452, Percent_Identity=43.8053097345133, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI22547186, Length=430, Percent_Identity=44.8837209302326, Blast_Score=334, Evalue=7e-92,
Organism=Homo sapiens, GI22547189, Length=414, Percent_Identity=43.4782608695652, Blast_Score=306, Evalue=2e-83,
Organism=Escherichia coli, GI1788902, Length=404, Percent_Identity=57.4257425742574, Blast_Score=460, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI25144732, Length=408, Percent_Identity=46.8137254901961, Blast_Score=357, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI25144729, Length=408, Percent_Identity=46.8137254901961, Blast_Score=357, Evalue=6e-99,
Organism=Saccharomyces cerevisiae, GI6319739, Length=402, Percent_Identity=47.5124378109453, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323087, Length=401, Percent_Identity=44.6384039900249, Blast_Score=327, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24640005, Length=451, Percent_Identity=44.7893569844789, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI221329721, Length=451, Percent_Identity=44.5676274944568, Blast_Score=373, Evalue=1e-103,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_HALOH (B8CYJ3)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002509357.1
- ProteinModelPortal:   B8CYJ3
- SMR:   B8CYJ3
- GeneID:   7312649
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_16130
- HOGENOM:   HBG301263
- OMA:   PRITSGI
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45546; Mature: 45546

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFLKKVDPDIFGLIEEEDQRQRRNIELIASENFVSDAVMEAAGSCLTNKYAEGYPHKRY
CCHHHHCCCHHEECCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
YGGCEVVDKVEELAIARARELFGAEHVNVQPHSGSQANQAVYFATVPPGGTILAMDLTHG
CCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEEEECCCCCEEEEEEECCC
GHLTHGSPVNMSGKYYNFIHYGVTREEEVIDFDQVRELARKHQPDLIVAGASAYPRIIDF
CCCCCCCCCCCCCCEEEEEEECCCCCHHHCCHHHHHHHHHHCCCCEEEECCCCCCCCCCH
EVFREIADEVGALFMVDMAHIAGLVAAGLHPSPVPVADFVTTTTHKTLRGTRGGLILCKG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECC
EHAKKIDKAIFPGLQGGPLLHIIAAKAVTFKEALQDEFKGYQKQIVSNAKTMAAELKNYG
CHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FRLVSGGTDNHLMLVDLTNMDITGKDAETALDKVGITVNKNTIPFEKRSPFVTSGIRIGT
EEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCEEECCCCCCCCCCCCCEECCEEECC
PAVTTRGMKEKEMKLIAKLIYQTLKNINDEGRLTEIRQEVYRLSERFPKTAE
CCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEFLKKVDPDIFGLIEEEDQRQRRNIELIASENFVSDAVMEAAGSCLTNKYAEGYPHKRY
CCHHHHCCCHHEECCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
YGGCEVVDKVEELAIARARELFGAEHVNVQPHSGSQANQAVYFATVPPGGTILAMDLTHG
CCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEEEECCCCCEEEEEEECCC
GHLTHGSPVNMSGKYYNFIHYGVTREEEVIDFDQVRELARKHQPDLIVAGASAYPRIIDF
CCCCCCCCCCCCCCEEEEEEECCCCCHHHCCHHHHHHHHHHCCCCEEEECCCCCCCCCCH
EVFREIADEVGALFMVDMAHIAGLVAAGLHPSPVPVADFVTTTTHKTLRGTRGGLILCKG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECC
EHAKKIDKAIFPGLQGGPLLHIIAAKAVTFKEALQDEFKGYQKQIVSNAKTMAAELKNYG
CHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FRLVSGGTDNHLMLVDLTNMDITGKDAETALDKVGITVNKNTIPFEKRSPFVTSGIRIGT
EEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCEEECCCCCCCCCCCCCEECCEEECC
PAVTTRGMKEKEMKLIAKLIYQTLKNINDEGRLTEIRQEVYRLSERFPKTAE
CCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA